This study performed a questionnaire survey among a total of 209 middle-aged women in their 40-65. The data were collected through convenience sampling. With the collected data, the effects of daily stress, depression and self-esteem on quality of life were investigated. For this, t-test, ANOVA and post-hoc test were conducted and correlation analysis was carried out to examine how the above three variables are correlated to each other. In addition, multiple regression analysis was applied to investigate the factors affecting quality of life and the study results found the following: First, daily stress showed a statistically significant correlation with age, marital status, family type, health condition, menopausal status, social activity and religion. Second, in depression, a significant correlation was found with health condition and social activity. Third, self-esteem was correlated with age, marital status, family type, health condition, social activity and religion with statistical significance. Fourth, in quality of life, a statistically significant correlation was observed with age, marital status, income, family type, health condition, social activity and religion. Fifth, quality of life showed a statistically significant correlation with age, marital status, income, family type, health condition, social activity and religion, Sixth, in correlations among the quality of life, depress, self-esteem and daily stress, quality of life was higher as daily stress and self-esteem increased while depression was lower. This study has confirmed that daily stress, depression and self-esteem have an influence on the quality of life in middle-aged women. Further studies are needed on the development of a more structured model with the factors affecting the quality of life in middle-aged women.