Effects Of Combinations Research Articles

Effects of Nutraceutical Compositions Containing Rhizoma Gastrodiae or Lipoic Acid in an In Vitro Induced Neuropathic Pain Model.

Peripheral neuropathy is caused by a malfunction in the axons and myelin sheaths of peripheral nerves and motor and sensory neurons. In this context, nonpharmacological treatments with antioxidant potential have attracted much attention due to the issues that some conventional pharmaceutical therapy can generate. Most of these treatments contain lipoic acid, but issues have emerged regarding its use. Considering this, the present study evaluated the beneficial effects of nutraceuticals based on Gastrodiae elata dry extract 10:1 or lipoic acid in combination with other substances (such as citicholine, B vitamins, and acetyl L-carnitine). To assess the combination's absorption and biodistribution and exclude cytotoxicity, its bioavailability was first examined in a 3D intestinal barrier model that replicated oral ingestion. Subsequently, a 3D model of nerve tissue was constructed to investigate the impacts of the new combination on the significant pathways dysregulated in peripheral neuropathy. Our findings show that the novel combination outperformed in initial pain relief response and in recovering the mechanism of nerve healing following Schwann cell injury by successfully crossing the gut barrier and reaching the target site. This article describes a potential alternative nutraceutical approach supporting the effectiveness of combinations with Gastrodiae elata extract in decreasing neuropathy and regulating pain pathways.

Enhancing Energy Efficiency in IoT-NDN via Parameter Optimization

The IoT encompasses objects, sensors, and everyday items not typically considered computers. IoT devices are subject to severe energy, memory, and computation power constraints. Employing NDN for the IoT is a recent approach to accommodate these issues. To gain a deeper insight into how different network parameters affect energy consumption, analyzing a range of parameters using hyperparameter optimization seems reasonable. The experiments from this work’s ndnSIM-based hyperparameter setup indicate that the data packet size has the most significant impact on energy consumption, followed by the caching scheme, caching strategy, and finally, the forwarding strategy. The energy footprint of these parameters is orders of magnitude apart. Surprisingly, the packet request sequence influences the caching parameters’ energy footprint more than the graph size and topology. Regarding energy consumption, the results indicate that data compression may be more relevant than expected, and caching may be more significant than the forwarding strategy. The framework for ndnSIM developed in this work can be used to simulate NDN networks more efficiently. Furthermore, the work presents a valuable basis for further research on the effect of specific parameter combinations not examined before.

Synergistic potential of α-Phellandrene combined with conventional antifungal agents and its mechanism against antibiotic resistant Candida albicans

Candida albicans is resistant to various antifungal drugs, this presents a significant problem on a global scale. This study investigates a novel approach on the potential fungicidal effects of α-Phellandrene combinations with fluconazole and amphotericin B against antibiotic resistant C. albicans. The agar well diffusion experiment was used to measure the anti-candida activity of α-Phellandrene which exhibited a zone of inhibition of 24 ± 0.5 mm and 22 ± 0.5 mm against the C. albicans cells (MTCC277 and ATCC90028), respectively. Additionally, the fungicidal minimum inhibitory concentration (MIC) ranged 0.0312–0.0156 mg/ml (w/v) against C. albicans strains. It was determined to have powerful and efficient antifungal action against Candida cells. Further, the synergistic potential was evaluated by employing a time kill assay and a checkerboard technique, respectively, which revealed after 16 h, the colony count of C. albicans cells ATCC90028 (2.56 ± 0.33) and MTCC277 (2.53 ± 0.33) dropped by a log10 when treated with a combination of α-Phellandrene and Fluconazole and α-Phellandrene and amphotericin B exhibited synergy against both C. albicans strains ATCC90028 and MTCC277 (2.42 ± 0.28 and 2.00 ± 0.21) log10 reduction in colony count, respectively, Additionally, 16–624-fold increase in the antifungal efficacy of clinical medicines, with total cell death occurring after 16 h. α-Phellandrene and antifungal drugs were tested in combination with the osmoprotectant test, ergosterol test and FESEM observations to determine their modes of action. In the era of multidrug-resistant diseases antibiotic resistance can be curtailed in its tracks with the help of combination treatments that allow for lower drug doses.

Effect of Organic and Inorganic Fertilizers on Growth, Nutrient Uptake and Yield of Okra (Abelmoschus esculentus L. Moench) in Bundelkhand

The present investigation was carried out at Organic Research Farm, Department of Horticulture, Institute of Agricultural Sciences, Bundelkhand University, Karguanji, Jhansi, Uttar Pradesh during the Kharif-2023 with a view to identify the effects of different combinations of organic and inorganic fertilizers and its role in growth, yield and quality of Okra variety Pusa A4. The experiment was laid in Randomized block design with 9 treatments and 3 replications with different combination in RDF and application of organic nutrition. Under this experiment, overall, 9 treatment was taken T0 Control (water spray), T1 (Ash 100%), T2 (MOP 100%), T3 (MOP 50% + Ash 50%), T4 (Presmud 5 t/ha), T6 (Presmud 1.5 t/ha), T7 (MOP 50% + Presmud 50%) and T8 (Presmud 50% + Ash 50%). From the above experimental finding it may be concluded that the treatment T2 (MOP 100%) was found to be best in the terms of growth yield and quality of Okra. The highest net return was found in the T2 (MOP 100%) with (Rs 111378/ha) and the highest B: C ratio was found in the same with 2.11.

A deepfake-based study on facial expressiveness and social outcomes

Artificial intelligence (AI)-generated media is used in entertainment, art, education, and marketing. AI-generated faces or facial expressions using deepfake-based technologies might also contribute to nonverbal behavior studies. As a proof of concept, in this research, we test the replicability of past results regarding the positive effects of facial expressiveness (e.g., gazing, nodding, and smiling) on social outcomes. Because the facial expressions when listening to someone (i.e., nonverbal immediacy) encompass several behaviors that are typically related (i.e., smiling while nodding), the effect of combinations of these behaviors is challenging to study. We thus test the effect of facial expressiveness (gazing, nodding, and smiling vs. none) on first impression judgements in a job interview setting using AI-generated experimental video material. We measure how competent, warm, and favorably independent observers (n = 823) perceive the targets (n = 159) showing AI-generated facial expressions. Our results replicate past research showing that the more targets are facially expressive, the more they are perceived favorably. Apart from supporting evidence of the importance of facial expressiveness for conveying a positive impression, we show the benefits of relying on AI-generated experimental video material for the study of nonverbal behavior.

Effects of sensory combination on crispness and prediction of sensory evaluation value by Gaussian process regression.

Crispness contributes to the pleasantness and enjoyment of eating foods and is popular with people of wide ages in many countries. Hence, a quantitative evaluation method for crispness is required for food companies developing new food products. In this study, the effects of different sensory combinations on crispness were investigated through sensory evaluation, and a Gaussian process regression model was used to predict the evaluation values of crispness. First, four crispness descriptors in Japanese were selected, and sensory evaluations were conducted with ten participants using commercially available snack foods under three different sensory combinations of force, vibration, and sound to confirm the effects of the three senses. An instrumental system also measured force, vibration, and sound for snack foods under the same conditions. The Gaussian process regression model determined the relationship between the sensory and measurement data and predicted the sensory evaluation values from the measurement data. Cross-validation verified that the Gaussian process regression model accurately predicted the food texture evaluation values from the measurement data even in conditions with different sensory components.

Impact of repeated fumigant applications on soil properties, crop yield, and microbial communities in a plastic-mulched tomato production system

Pre-plant soil fumigation is widely applied to control nematodes, soil-borne fungal pathogens, and weeds in vegetable crops. However, most of the research evaluating the effect of fumigants on crop yield and soil microbial communities has been done on single compounds despite growers mainly applying fumigant combinations. We studied the effect of different fumigant combinations (chloropicrin, 1,3-dichloropropene, and metam potassium) on soil properties, crop yield, and the soil bacterial and fungal microbiome for two consecutive years in a plastic-mulched tomato production system in Florida (United States). While combinations of fumigants did not improve plant productivity more than the individual application of these products, application of fumigants with >60 % chloropicrin did significantly increase yield. Fumigant combinations had no significant effect on bacterial diversity, but fumigants with >35 % chloropicrin reduced soil fungal diversity and induced temporary changes in the soil bacterial and fungal community composition. These changes included short-term increases in the relative abundance of Firmicutes and Ascomycota, as well as decreases in other bacterial and fungal taxa. Repeated fumigation reduced network complexity and the relative abundance of several predicted bacterial functions and fungal guilds, particularly after fumigation and at end of harvest (3-months post fumigation). A structural equation model (SEM) showed fumigants not only directly impact crop yield, but they can also indirectly determine variations in plant productivity through effects on the soil microbiome. Overall, this study increases our understanding of the environmental and agricultural impacts of fumigants in a plastic-mulched tomato production system.

Research on different compound combinations of Realgar-Indigo naturalis formula to reverse acute promyelocytic leukemia arsenic resistance by regulating autophagy through mTOR pathway

Ethnopharmacological relevanceIn China, the Chinese patent drug Realgar-Indigo naturalis Formula (RIF) is utilized for the therapy of acute promyelocytic leukemia (APL). Comprising four traditional Chinese herb—Realgar, Indigo naturalis, Salvia miltiorrhiza, and Pseudostellaria heterophylla—it notably includes tetra-arsenic tetra-sulfide, indirubin, tanshinone IIa, and total saponins of Radix Pseudostellariae as its primary active components. Due to its arsenic content, RIF distinctly contributes to the therapy for APL. However, the challenge of arsenic resistance in APL patients complicates the clinical use of arsenic agents. Interestingly, RIF demonstrates a high remission rate in APL patients, suggesting that its efficacy is not significantly compromised by arsenic resistance. Yet, the current state of research on RIF's ability to reverse arsenic resistance remains unclear. Aim of the studyTo investigate the mechanism of different combinations of the compound of RIF in reversing arsenic resistance in APL. Materials and methodsThe present study utilized the arsenic-resistant HL60-PMLA216V-RARα cell line to investigate the effects of various RIF compounds, namely tetra-arsenic tetra-sulfide (A), indirubin (I), tanshinone IIa (T), and total saponins of Radix Pseudostellariae (S). The assessment of cell viability, observation of cell morphology, and evaluation of cell apoptosis were performed. Furthermore, the mitochondrial membrane potential, changes in the levels of PMLA216V-RARα, apoptosis-related factors, and the PI3K/AKT/mTOR pathway were examined, along with autophagy in all experimental groups. Meanwhile, we observed the changes about autophagy after blocking the PI3K or mTOR pathway. ResultsTanshinone IIa, indirubin and total saponins of Radix Pseudostellariae could enhance the effect of tetra-arsenic tetra-sulfide down-regulating PMLA216V-RARα, and the mechanism was suggested to be related to inhibiting mTOR pathway to activate autophagy. ConclusionsWe illustrated that the synergistic effect of different compound combinations of RIF can regulate autophagy through the mTOR pathway, enhance cell apoptosis, and degrade arsenic-resistant PMLA216V-RARα.

Therapeutic effects of rehabilitation technologies in patients with functional asthenia

Relevance: determining the therapeutic effects of a complex of pathogenetically determined rehabilitation technologies in patients with functional asthenia is an urgent scientific and practical task. The aim of the study was to determine the therapeutic effects of combinations of rehabilitation technologies in patients with functional asthenia. Methods: 24 patients aged 29.3 (21.5–35.0) years with functional asthenia (ICD-10 code: R53) were studied. The patients were prescribed a set of physical exercises, a course of audiovisual polysensory relaxation, carbon dioxide baths, climatotherapy, clinical nutrition, and psychotherapy. The total duration of the course was 30 days. Clinical, instrumental, psychophysical, and integral methods of assessing the condition of patients were used. Results: an improvement in clinical status indicators and a decrease in signs of asthenia with an improvement in mood, well-being, cheerfulness, and self-confidence were noted. The rehabilitation course caused a pronounced oxygen capacity of tissues, an increase in adaptation reserves, an improvement in vegetative regulation and the level of somatic health, and a significant increase in physical performance in patients with functional asthenia. Conclusion: а complex of physical exercises, audiovisual polysensory relaxation, carbon dioxide baths, climatotherapy, clinical nutrition, and psychotherapy forms pronounced actoprotective and psychocorrective therapeutic effects in patients with functional asthenia.

Efficacy of flossing and mouth rinsing regimens on plaque and gingivitis: a randomized clinical trial

BackgroundTo investigate the effects of combinations of mechanical (brushing and flossing) and chemotherapeutic regimens which included essential oils (EO) non-alcohol and alcohol-containing mouthrinses compared to brushing only in the prevention and reduction of plaque, gingivitis, and gingival bleeding.MethodsThis was a randomized, virtually supervised, examiner blind, controlled clinical trial. Following informed consent and screening, subjects (N = 270) with gingivitis were randomly assigned to one of the following regimens: (1) Brush Only (B, n = 54); (2) Brush/Rinse (EO alcohol-containing mouthrinse) (BA, n = 54); (3) Brush/Rinse (EO non-alcohol containing mouthrinse) (BZ, n = 54); (4) Brush/Floss (BF, n = 54); (5) Brush/Floss/Rinse (EO non-alcohol containing mouthrinse) (BFZ, n = 54). Unflavored waxed dental floss (REACH unflavored waxed dental floss), and fluoridated toothpaste (Colgate Cavity Protection) were used. Examinations included oral hard and soft tissue, plaque, gingivitis, gingival bleeding, probing depth and bleeding on probing.ResultsAfter 12 weeks, both BA and BZ and the BFZ group were superior in reducing interproximal plaque (30.8%, 18.2%, 16.0%, respectively), gingivitis (39.0%, 36.9%, 36.1%, respectively), and bleeding (67.8%, 73.6%, 79.8%, respectively) compared to B. The BF group did not provide significant reductions in interproximal plaque but did reduce interproximal gingivitis (5.1%, p = 0.041) at Week 4 and bleeding at Weeks 4 and 12 (34.6%, 31.4%, p < 0.001 respectively) compared to B. The BFZ group did not significantly reduce interproximal plaque, gingivitis or bleeding compared to BZ.ConclusionsThis study demonstrated that the addition of EO non-alcohol containing mouthrinse to the manual toothbrushing and flossing regimen further reduces plaque, gingivitis and bleeding showing that addition of EO mouthrinses (alcohol or non-alcohol containing) to the oral hygiene regimen provides sustained reductions in plaque to help maintain gingival health after a dental prophylaxis. Dental professional recommendation of the addition of an EO non-alcohol containing mouthrinse to daily oral hygiene routines of brushing or brushing and flossing should be considered to aid supragingival plaque control and improve gingivitis prevention.Study registry numberNCT05600231.

Efficacy of herbicides and reductant at different levels to control invasive tall reed (Phragmites karka) in oil palm plantations

Controlling Phragmites karka, a highly invasive weed, is critical for peat areas since this species is a significantand hard-to control weed in oil palm plantations. Herbicides are one of the most effective and efficient tools for controlling P. karka, and their use is increasing due to their ability to provide rapid and long-term results. However, herbicides must be applied responsibly and carefully to minimize any environmental impacts. The study included an evaluation of the effectiveness of several combinations of herbicides on P. karka in an oil palm plantation and a comparison of their efficiency and cost. Results showed that combination of 1.8 L/ha glyphosate, 0.045 L/ha topramezone and 0.3 L/ha reductant Y (G7) has a promising result in controlling P. karka (weed mortality of 97% until 8 WAA and 12% regrowth until 12 WAA) and was more cost-effective compared to estate practice, reducing cost up to 0.43% per hectare because of the use of pesticide reductant. The use of reductant technology in this treatment also showed an effectiveness in reducing application frequency. This study demonstrates that herbicides can effectively and efficiently control P. karka in oil palm plantations, especially in peat land. Reductant can be one of the solutions to reduce the use of herbicides without lessening the efficacy, affordably, and sustainably.

Reaping what you sow: A multilevel investigation of allocation from a pool of workers to teams.

Organizations commonly face the task of allocating workers to mutually exclusive teams from finite worker pools-a process called seeding. The approach an organization takes to seeding affects within-team and between-team distributions of performance or other outcomes. Substantial prior research explains the effects of combinations on team performance, but little is known about between-team combinations. I extend prior theory to a higher level of analysis, elaborating on the nature and function of between-team combinations on organization-level performance. I use a simulation method to identify seeding approaches that can maximize organizational outcomes in various contexts. Results uncover conditions under which the seeding approach is irrelevant to outcomes, instances where random assignment outperforms intentional seeding, and instances where particular approaches produce the most favorable outcomes. I discuss the implications of multilevel combinations for theory and practice. (PsycInfo Database Record (c) 2023 APA, all rights reserved).

Flow velocity and light intensity combination is important for Microcystis aeruginosa physical suppression.

The cyanobacterial response to flow velocity or light intensity deviates from the combined effect of both factors. The responses of Microcystis aeruginosa to different combinations of flow velocities and light intensities were tested. Growth (OD730 and protein), stress (catalase, ascorbate peroxidase, and glutathione peroxidase), and photosynthetic ability (chlorophyll-a and fluorescence) parameters of M.aeruginosa were measured to evaluate the effects of different combinations. Exposure to different flow velocity-light combinations significantly affected the growth and physiology of M.aeruginosa. Flow velocities of 0.4m s-1 showed a prominent influence on most of the measured parameters compared with no flow velocity or higher flow velocity conditions. The 1.2-m s-1 flow velocity and high light intensity (1200 μmol m-2 s-1 ) exposure caused a significant elevation in oxidative stress. Lower velocities are beneficial for M.aeruginosa at light stress, whereas extreme velocities are adverse and elevate the stress. Two categories of light-velocity combinations were identified as preferred and extreme categories, depending on whether they suppressed or supported M.aeruginosa growth. In controlling cyanobacteria blooms using flow or high-intensity light, it is imperative to consider the interaction of these two factors, as their combined effects can significantly vary the stress levels in cyanobacteria. A new system, designed to minimize mechanical damage on M.aeruginosa, was used to generate flow velocities. Additionally, the combined effects of flow velocities and light intensities have been considered for the first time. PRACTITIONER POINTS: Flow velocity can influence the effect of light on Microcystis aeruginosa. High light exposure effect on Microcystis aeruginosa can be reduced by low flow velocity. High flow velocity and high light exposure increase the stress on Microcystis aeruginosa. Different light intensities and flow velocity combinations changed Microcystis aeruginosa stress physiology.

Repurposing proteasome inhibitors for improved treatment of triple-negative breast cancer

Triple-negative breast cancer (TNBC) is associated with poor prognosis and limited treatment options due to the lack of important receptors (estrogen receptor [ER], progesterone receptor [PR], and human epidermal growth factor receptor 2 [HER2]) used for targeted therapy. However, high-throughput in vitro drug screening of cell lines is a powerful tool for identifying effective drugs for a disease. Here, we determine the intrinsic chemosensitivity of TNBC cell lines to proteasome inhibitors (PIs), thereby identifying potentially potent 2-drug combinations for TNBC. Eight TNBC cell lines (BT-549, CAL-148, HCC1806, HCC38, HCC70, MDA-MB-436, MDA-MB-453, and MDA-MB-468) and two controls (MCF-10A and MCF-7) were first exposed to 18 drugs (11 PIs and 7 clinically relevant chemotherapeutic agents) as monotherapy, followed by prediction of potent 2-drug combinations using the IDACombo pipeline. The synergistic effects of the 2-drug combinations were evaluated with SynergyFinder in four TNBC cell lines (CAL-148, HCC1806, HCC38, and MDA-MB-468) and three controls (BT-474, MCF-7, and T47D) in vitro, followed by further evaluation of tumor regression in zebrafish tumor models established using HCC1806 and MCF-7 cells. Monotherapy identified nine effective drugs (bortezomib, carfilzomib, cisplatin, delanzomib, docetaxel, epoxomicin, MLN-2238, MLN-9708, and nedaplatin) across all cell lines. PIs (e.g., bortezomib, delanzomib, and epoxomicin) were highly potent drugs in TNBC cells, of which bortezomib and delanzomib inhibited the chymotrypsin-like activity of the 20 S proteasome by 100% at 10 µM. Moreover, several potent 2-drug combinations (e.g., bortezomib+nedaplatin and epoxomicin+epirubicin) that killed virtually 100% of cells were also identified. Although HCC1806- and MCF-7-derived xenografts treated with bortezomib+nedaplatin and carboplatin+paclitaxel were smaller, HCC1806 cells frequently metastasized to the trunk region. Taken together, we show that PIs used in combination with platinum agents or topoisomerase inhibitors exhibit increased efficiency with almost 100% inhibition in TNBC cell lines, indicating that PIs are therefore promising compounds to use as combination therapy for TNBC.

Fucosylation inhibitor 6-alkynylfucose enhances the ATRA-induced differentiation effect on acute promyelocytic leukemia cells

For acute promyelocytic leukemia (APL), differentiation therapy with all-trans retinoic acid (ATRA) is well established. However, the narrow application and tolerance development of ATRA remain to be improved. In this study, we investigated the effects of combinations of glycosylation inhibitors with ATRA to achieve better efficiency than ATRA alone. We found that the combination of fucosylation inhibitor 6-alkynylfucose (6AF) and ATRA had an additional effect on cell differentiation, as revealed by expression changes in two differentiation markers, CD11b and CD11c, and significant morphological changes in NB4 APL and HL-60 acute myeloid leukemia (AML) cells. In AAL lectin blot analyses, ATRA or 6AF alone could decrease fucosylation, while their combination decreased fucosylation more efficiently. To clarify the molecular mechanism for the 6AF effect on ATRA-induced differentiation, we performed microarray analyses using NB4 cells. In a pathway analysis using DAVID software, we found that the C-type lectin receptor (CLR) signaling pathway was enriched with high significance. In real-time PCR analyses using NB4 and HL-60 cells, FcεRIγ, CLEC6A, CLEC7A, CASP1, IL-1β, and EGR3, as components of the CLR pathway, as well as CD45 and AKT3 were upregulated by 6AF in ATRA-induced differentiation. Taken together, the present findings suggest that the CLR signaling pathway is involved in the 6AF effect on ATRA-induced differentiation.

Strategic capital taxation, tradable emission permits and global pollution

AbstractThe paper considers a two‐country model with international capital mobility and production‐generated cross‐border pollution. It examines the effectiveness of alternative policy combinations consisting of capital taxes and nationally or internationally tradable emissions permits in reducing global pollution. The internationally mobile capital is taxed according to three different rules, namely, capital tax exemptions, capital tax credits and capital tax deductions. Our key result is that, under certain conditions, the lowest Nash equilibrium level of global pollution is achieved with a policy mix combining either capital tax exemptions or capital tax credits with internationally, rather than nationally, tradable emission permits.

In Vitro Combinatorial Activity of Direct Acting Antivirals and Monoclonal Antibodies against the Ancestral B.1 and BQ.1.1 SARS-CoV-2 Viral Variants.

Combination antiviral therapy may be helpful in the treatment of SARS-CoV-2 infection; however, no clinical trial data are available, and combined use of direct-acting antivirals (DAA) and monoclonal antibodies (mAb) has been reported only anecdotally. To assess the cooperative effects of dual drug combinations in vitro, we used a VERO E6 cell-based in vitro system with the ancestral B.1 or the highly divergent BQ.1.1 virus to test pairwise combinations of the licensed DAA, including nirmatrelvir (NRM), remdesivir (RDV) and the active metabolite of molnupiravir (EIDD-1931) as well the combination of RDV with four licensed mAbs (sotrovimab, bebtelovimab, cilgavimab, tixagevimab; tested only with the susceptible B.1 virus). According to SynergyFinder 3.0 summary and weighted scores, all the combinations had an additive effect. Within DAA/DAA combinations, paired scores with the B.1 and BQ.1.1 variants were comparable. In the post hoc analysis weighting synergy by concentrations, several cases of highly synergistic scores were detected at specific drug concentrations, both for DAA/DAA and for RDV/mAb combinations. This was supported by in vitro confirmation experiments showing a more than a linear shift of a drug-effective concentration (IC50) at increasing concentrations of the companion drug, although the effect was prominent with DAA/DAA combinations and minimal or null with RDV/mAb combinations. These results support the cooperative effects of dual drug combinations in vitro, which should be further investigated in animal models before introduction into the clinic.

Object aroused emotion analysis network for image sentiment analysis

From cognitive psychology, objects are closely related to emotions, and inherently possess the ability to arouse human emotions. Hence, fully utilizing the relationships between objects and emotions can help achieve more accurate visual emotion recognition. In this paper, we propose a novel object aroused emotion analysis network to realize image sentiment classification by investigating the interactions between objects and emotions. To quantify the various emotion potencies of each object, a novel object emotion distribution module is proposed to explore the mapping among objects and emotions, and quantitatively demonstrate how various objects arouse different emotions. An object emotion-modeling mapping module is proposed to analyze the effect of objects and object combinations with emotion information on image sentiment; this module maps common objects to emotion dimensions and improves image sentiment classification with abundant object combination information. Then, we fuse the object-emotion mapping relation and multi-model features using BiGRU, thus realizing more accurate emotion recognition. Extensive experiments on widely used emotion datasets prove that our proposed method achieves excellent performance and outperforms most state-of-the-art image sentiment classification methods.

Integrated use of NPK chemical fertilizers and bio-stimulants improved soil fertility, fruit yield, quality and net returns in pomegranate (Punica granatum L.)

Bio-stimulants are gaining importance for elevating the stagnated yield level resulting from continuous use of chemical fertilizers (CF) in many crops, however, their use in pomegranate have not been fully explored. Two years of field experiments were conducted on a 5-years old pomegranate cv. Bhagwa orchard to investigate the effects of different combinations of commercial bio-stimulant products namely, microbial-based product A containing consortium of Rhizophagus spp. (5 kg ha−1), seaweed extract-based granular formulation D (30 kg ha−1) and liquid formulation B (625 and 1250 ml ha−1) through soil application as well as wettable granular formulation, C (1 and 2 g l−1) and liquid formulation, E (1 ml l−1) through foliar sprays along with chemical fertilizers on soil fertility, trees’ nutrition, fruit yield and quality of pomegranate. Results showed that integrated use of CF and bio-stimulants significantly increased the number of bisexual flowers. However, the combination of microbial-based product A (5 kg ha−1), seaweed extract based liquid formulation B (625 ml ha−1) in soil and foliar spary of seaweed extract based wettable granular formulation C (1–2 g l−1) with CF resulted in production of the highest fruit yield (increased by 41.97–51.61%) with 23.08–33.38% increase in the exportable grade fruit and improved quality attributes viz. ascorbic acid, anthocyanin, non-reducing sugar, protein, and mineral content of fruits through enhancement in soil fertility and trees’ micronutrient status compared to only CF. These doses combinations of bio-stimulants along with CF gave rise to the highest benefit–cost ratio of 2.10–2.34:1 and hence can be recommended for sustainable pomegranate cultivation.

Co-exposure of carbon black and cadmium induces autophagy and inflammation in human bronchial epithelial cells via PERK pathway

Objective: To investigate the effects of carbon black and cadmium (Cd) combined exposure on autophagy and inflammatory response mediated by protein kinase R-like endoplasmic reticulum kinase (PERK) pathway in human bronchial epithelial (16HBE) cells. Methods: In January 2022, human bronchial epithelial (16HBE) cells were resuscitated and cultured. Carbon black nanoparticles (CBNPs) were oxidized to adsorb Cd ions to construct "CBNPs-Cd" complexes. CCK-8 assay was used to detect the effects of different concentrations and time combinations of CBNPs and Cd on the viability of 16HBE cells. The subsequent dose groups were exposed to 2 μg/ml Cd, 100 μg/ml CBNPs, 100 μg/ml CBNPs+2 μg/ml Cd for 24 h. The number of autophagosomes and autolysosomes was detected by transmission electron microscopy. Western blotting was used to detect the protein expressions of PERK, eukaryotic initiation factor 2α (eIf2α), activating transcription factor 4 (ATF4), sequestosome 1 (SQSTM1/P62), and microtubule-associated protein 1 light chain 3 (LC3). After PERK gene was silenced by siRNA technology, the changes of autophagy marker proteins P62 and LC3 were detected, and the expressions of inflammatory factors interleukin-6 (IL6) and interleukin-8 (IL8) were detected by fluorescence quantitative PCR technique. One-way ANOVA analysis was used to compare three groups or more. LSD test was used for comparison between two groups. Factorial analysis was used for multivariate component analysis. Results: There was no significant change in cell viability of 16HBE after 24 h exposure to CBNPs and Cd alone or combined (P>0.05). Compared with the control group, the expressions of P62 and LC3 in 16HBE cells were significantly increased in the CBNPs and Cd alone/combined exposure group (P<0.05), and the number of autophagosomes and autophagolysosomes in the combined exposure group was increased compared with other groups. Compared with the control group, CBNPs and Cd alone exposure group had no significant effects on p-PERK/PERK and p-eIf2α/eIf2α protein expression (P>0.05). However, the protein expressions of p-PERK/PERK and p-eIf2α/eIf2α and ATF4 were all increased in the combined exposure group (P<0.05), and the levels of IL6 and IL8 in 16HBE cells in the combined exposure group of CBNPs and Cd were significantly higher than those in the control group (P<0.05). The levels of LC3 protein, IL6 and IL8 were decreased in the CBNPs-Cd combined exposure group after knockdown of PERK gene (P<0.05). The results of factorial analysis showed that exposure to CBNPs and Cd had significant effects on the expression of P62, LC3 and IL6 (P<0.05), but the interaction between the two chemicals had no statistical significance (P>0.05) . Conclusion: CBNPs-Cd combined exposure may inhibit autophagy and increase inflammation in human bronchial epithelial cells through activation of PERK-eIf2α-ATF4 pathway.