Effect Of Catechol Research Articles

Effect of catechol and disodium EDTA on melanin content of hyphal and sclerotial walls of Sclerotium rolfsii sacc. and the role of melanin in the susceptibility of these walls to β-(1 → 3) glucanase and chitinase

Cell walls prepared from hyphae and from the outer layers of sclerotia of Sclerotium rolfsii Sacc. grown in the presence or absence of catechol which induces melanin deposition in the hyphal walls or disodiumethylene-diaminetetracetic acid (Na 2EDTA) which prevents melanin formation by the sclerotia, were compared for their susceptibility to β-(1 → 3) glucanase and chitinasc. A bacterial β-(1 → 3) endo-glucanase solubilized 49.5% of insoluble lammarin, 25.2 and 6.3% of the total dry weight of ordinary and melanin-containing hyphal walls respectively, and 5.9 and 10.3% of the total dry weight of ordinary and melanin-free sclerotial walls respectively, after 24 hr of incubation at 37°C. It was found that 2.2, 0.7, 0.8 and 1.3% of the total dry weight were solubilized by chitinase after 2 hr from ordinary hyphal walls, melanin-containing hyphal walls, ordinary slecrotial walls and melanin-free sclerotial walls, respectively. When added to the reaction mixtures, melanin extracted from catechol-grown hyphae and from sclerotial walls of S. rolfsii did not affect the activity of chitinase and glucanase. It was concluded that the susceptibility of various preparations of S. rolfsii walls to glucanase and chitinase is dependent upon both their physical structure and their melanin content.

The effect of phenolic compounds on growth, polygalacturonase production and activity of Fusarium oxysporum f. vasinfectum

The effect of catechol, phloroglucinol, gallic acid, phloretin and catechin on the growth ofFusarium oxysporum f.vasinfectum, on the ability of the fungus to produce polygalacturonase (PG) and on the activity of this enzyme has been investigated.

Further studies on the effect of catechol on the isolated guinea-pig ileum.

AbstractCatechol in doses of 0.1–2 mg added to the isolated guinea‐pig ileum in a 15 ml bath usually induced a contraction of the strip. Atropine and paralyzing doses of cocaine and hexamethonium abolished this contraction. BOL and phenbenzamine had no effect on contractions due to catechol. Catechol in non‐stimulating doses reduced the inhibition caused by adrenaline and noradrenaline. It is suggested that catechol like nicotine stimulates the gut through the mediation of acetylcholine and stimulation of the intramural ganglia.

The Effect of Catechol on the Isolated Guinea‐pig Ileum

AbstractCatechol in doses of 0.5‐5 mg, added to the isolated guinea‐pig ileum in a 15 ml bath, was generally found to induce contraction of the strip. Atropine and paralysing doses of nicotine abolished this contraction. Catechol in doses of 5–10 mg added to the organ bath had a relaxing action on the ileum. The probable way of stimulation via the intestinal ganglia is discussed.

Polyphenoloxidase and the Respiration of Ivy Leaves

SUMMARY 1. Polyphenoloxidase is present in ivy leaves but not in those of Aucuba or Euonymus. 2. Respiration of intact ivy leaves was markedly stimulated by catechol (R.Q. approximately = i), gallic acid, caffeic acid, and dihydroxyphenylalanine. The stimulation was not related to injury as far as could be detected and was not followed by inhibition. The extra oxygen consumption represents a many times repeated oxidation of the amount of catechol supplied. 3. The effect of catechol on the respiration of Aucuba and Euonymus leaves was very small. 4. Cupferron and phenylthiourea, which inhibit polyphenoloxidase in vitro, nevertheless increased respiration when administered to leaves through the petiole. On the other hand, when applied by infiltration, cupferron did cause inhibition, but in Aucuba and Euonymus as much as in ivy.