Effect Of Aqueous Ethanol Extract Research Articles

Aqueous Ethanolic Extract of Inonotus obliquus Ameliorates Polycystic Ovarian Syndrome by Modulating Oxidative Stress and Downregulating TNF-α and Interleukin-6

Polycystic ovarian syndrome (PCOS) is a complex metabolic and endocrine disorder with multifactorial etiology and complex pathogenesis. These intrinsic physiological changes cause anovulation, infertility, and miscarriage in women and exacerbate their chances of becoming hyperlipidemic and diabetic. Inonotus obliquus has been used traditionally for infertility problems. The present study aimed to investigate the therapeutic effects of aqueous ethanolic extract of Inonotus obliquus (AEIO) in female rats with letrozole-induced PCOS, along with the determination of its possible mechanism. HPLC analysis revealed the presence of gallic acid, chlorogenic acid, rutin, p-coumaric, benzoic acid, quercetin, salicylic acid, and kaempferol. Thirty female albino rats were acquired and divided into two groups (5 + 25) to induce PCOS. Letrozole (1 mg/kg) was used to induce the disease for 7 weeks (25 rats) except for the normal control (5 rats). The disease was confirmed by vaginal smear cytology, weight gain, and endocrinopathy. After disease induction, rats were divided into five groups (five rats in each group; disease control, metformin 20 mg/kg, AEIO 100, 300, and 500 mg/kg). After completion of the study, the animals were euthanized under the influence of anesthesia (chloroform). Ovaries were removed for histopathology, the liver was evaluated for oxidative stress biomarkers, and blood samples were collected for biochemical evaluation. Ovarian histopathology showed an abnormal architecture with cystic follicles and abnormal granulosa cells. Interestingly, treatment with AEIO restored normal ovarian histology with primary, growing, and developing follicles. After conducting a hormonal analysis, it was found that the induction of PCOS led to a significant increase (p < 0.001) in luteinizing hormone (LH), insulin, and testosterone levels, while the levels of follicle-stimulating hormone (FSH) decreased. However, treatment with AEIO at doses of 100–500 mg/kg restored these levels to normal. PCOS induction also resulted in oxidative stress and lipid peroxidation by significantly decreasing antioxidant enzymatic markers (SOD, CAT, and GSH) and increasing levels of lipid peroxidation enzymatic markers (MDA). AEIO restored the levels of superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH), and reduced the level of malondialdehyde (MDA). In conclusion, antioxidant phytochemicals (gallic acid, chlorogenic acid, rutin, p-coumaric, benzoic acid, quercetin, salicylic acid, and kaempferol) rich extract alleviated PCOS symptomatology through modulating oxidative stress markers and eliminating ovarian low-grade inflammation by downregulating the expression of NF-κB associated TNF-α and IL-6.

Anti-ulcer activity of aqueous ethanol extract of Rheum spiciforme and its fractions in animal model

Helicobacter pylori infection, pepsin, ischemia, hypoxia, smoking, alcohol intake, hydrochloric acid, and nonsteroidal anti-inflammatory drugs are the causal factors of ulceration. In third-world countries, approximately one in two individuals has signs of gastric ulceration. Extensive literature suggested that plant-derived drugs hold the potential in the treatment of peptic ulcer, and Rheum spiciforme (family: Polygonaceae) has been known in the folk medicine for possessing medicinal effect on ulceration. To explore further along this line, the current study was designed to investigate the effects of aqueous ethanol extract of R. spiciforme roots and its fractions on peptic ulcer and to identify bioactive compounds responsible for mitigating this pathological condition. Animal model of ethanol-induced gastric ulcer was utilized in this study. The animals were pre-treated with crude plant extract, given at doses 125, 250, and 500 mg/kg, and then orally administered with butanol, aqueous, and dichloromethane fractions at doses of 250 mg/kg for 7 days. Following the treatments, a significant decrease in ulcerative lesions, ulcer index, ulcer severity score, volume, free acidity, and total acidity of gastric juice as well as a marked increase of gastric pH in the groups treated with plant extract at doses of 250 and 500 mg/kg were noted. Moreover, butanol fraction has been shown to produce effects equipotent to that of the reference drug omeprazole. Liquid chromatography-mass spectrometry analyses show that the plant extract contains emodin, aloe-emodin, and quercetin. In conclusion, this study demonstrated that the aqueous ethanol extract of R. spiciforme and its butanol fraction exhibited gastro-protective effect.

Effect of Aqueous Ethanolic Extract of Andrographis paniculata and Adathoda vasica and Role of Trace Elements in Type 2 Diabetic Rat Model

Effect of Aqueous Ethanolic Extract of Andrographis paniculata and Adathoda vasica and Role of Trace Elements in Type 2 Diabetic Rat Model

Hypolipidemic and antioxidant effects of aqueous ethanolic extract from Moringa peregrina leaves on high-fat diet-induced fatty liver disease in rabbits

Hypolipidemic and antioxidant effects of aqueous ethanolic extract from Moringa peregrina leaves on high-fat diet-induced fatty liver disease in rabbits

Effects of aqueous ethanolic ficus deltoidea var. kunstleri extract on lipid profile and inflammation in high cholesterol diet-induced atherosclerotic rabbits

Background and Aims: Ficus deltoidea var. kunstleri (FDK), a natural product has been reported to exhibit atheroprotective properties in vitro. However, its in vivo atheroprotective effects are unknown. This study aims to investigate the atheroprotective effects of aqueous ethanolic extract of FDK on high cholesterol diet (HCD)-induced early and established atherosclerosis in rabbits.

Cocoyam (Colocasia esculenta) modulates some parameters of testosterone propionate-induced rat model of benign prostatic hyperplasia

The increased global prevalence of benign prostatic hyperplasia (BPH) and the promising potentials of functional foods in ameliorating it led to this study which reported the effect of aqueous ethanol extract of cocoyam (Colocasia esculenta) tuber on some biochemical indices in testosterone propionate (TP) induced benign prostatic hyperplasia (BPH) rats. Thirty male albino rats were randomly assigned into 6 groups of 5 rats each. Group 1 (negative control) received 3 mg/kg of TP and normal saline, group 2 (positive control) received 3 mg/kg of TP and 5 mg/kg of finasteride; groups 3, 4, and 6 rats received 3 mg/kg of TP and 100, 200 and 400 mg/kg of ethanol extracts of cocoyam respectively while group 5 (normal control) received olive oil + normal saline. The study lasted for 28 days. The negative control had increased prostate weight (p < 0.05), decreased body weight gain, prostatic superoxide dismutase, catalase and glutathione concentrations; no differences (p > 0.05) in the serum total cholesterol, triacylglycerol, Very Low Density Lipoprotein, High Density Lipoprotein, Low Density Lipoprotein concentration but increased (p < 0.05) prostate levels of interleukin 10, prostate specific antigen, testosterone, total proteins and malondialdehyde relative to the normal control. Finasteride or the C. esculenta tuber extract modulated most of these parameters as corroborated by histology of the prostate. The percentage yield of the C. esculenta tuber extract was 1.56% and 23 phenolic compounds were characterized in the tuber. The study showed the potentials of C. esculenta tuber in the management of BPH.

Antiulcerogenic effect of Cuphea ignea extract against ethanol-induced gastric ulcer in rats

BackgroundCuphea ignea is one of the herbal resources belonging to Lythraceae family. Some species of this family have been used traditionally in South and Central America’s folk medicine for treating stomach disorders. Therefore, the present study was performed to evaluate the gastropreventive effect of aqueous ethanolic extract of C. ignea aerial parts on ethanol-induced gastric ulcer.MethodsGastric ulcers were induced in Sprague Dawley rats using one oral dose of absolute ethanol (1.5 mL/rat). The C. ignea aerial parts extract at doses of 250 and 500 mg/kg body weight and ranitidine (a reference drug) at a dose of 30 mg/kg body weight were orally administrated daily for 7 days before ulcer induction. One hour after ethanol administration blood samples were collected and then stomachs of sacrificed rats were subjected to biochemical, macroscopic and microscopic studies.ResultsOral administration of C. ignea extract significantly attenuated gastric ulcer as revealed by significant reduction in the gastric ulcer index and volume of gastric juice while significantly increased preventive percentage, gastric pH value and pepsin activity. Pre-treatment of C. ignea extract markedly improved the serum level of TNF-α, the gastric MPO activity and NO content. Furthermore, C. ignea pre-treatment significantly increased the gastric levels of enzymatic and non- enzymatic antioxidants namely CAT, SOD, GSH-Px, and GSH with concomitant reduction in MDA level compared with those in the ethanol group. These results were further supported by histopathological findings which revealed the curing effect of C. ignea on the hemorrhagic shock induced by ethanol toxicity.ConclusionsC. ignea extract showed a potential gastroprotective effect on ethanol-induced gastric ulcer, and its effect may be mediated through suppression of oxidative stress and gastric inflammation.

WOUND HEALING ACTIVITY OF THE WHOLE PLANT OF MOMORDICA CHARANTIA LINN. IN RATS

The aim of the present study was to explore the effect of wound healing activity of the whole plant of Momordica charantia Linn. in rats. The effect of aqueous ethanolic extract of Momordica charantia Linn. (Family; Cucurbetaceae) was evaluated on resutured incision, granuloma pouch and excision wound models in rats. The wound healing activity was measured by the rate of wound contraction, skin breaking strength, weight of the granulation tissue, and collagen content. The concentrated ethyl alcohol extract was subject to fractionation by using petroleum ether (40-60o V/V), solvent ether, ethyl acetate, butanol, butanone, ethanol and methanol in succession. All the fractions were screened for wound healing property. The results of pharmacological activity led to the conclusion that the petroleum ether fraction exhibited more significant wound healing promotion property followed by solvent ether and butanone, from all the parameters studied. Ethyl acetate, ethanol and methanol fractions were least effective.

Effect of aqueous ethanol extract of Dialium guineense leaf on diclofenac-induced oxidative stress and hepatorenal injuries in Wistar rats

Drug-induced liver and kidney injuries are threats to public health worldwide, but there are claims that medicinal plants may provide remedy. Protective effect of Dialium guineense leaf extract (DGE) against diclofenac (DF)-induced oxidative injuries to the liver and kidney was evaluated in rats. Extract was prepared by maceration of pulverized sample in a mixture of water and ethanol, filtration, and evaporation to dryness. Eighteen rats were divided into three groups with six rats per group. Group I rats received normal saline, group II rats received 10 mg/kg DF by intramuscular route for 7 days while group III rats were treated with 250 mg/kg DGE orally for 28 days and given DF as group II rats. At the end of treatment, blood was collected from each rat and the serum was separated and used for spectrophotometric estimations of biomarkers of hepatorenal injuries. Liver and kidneys were excised from euthanized rats, homogenized in phosphate buffer and the supernatants used for assays of biomarkers of oxidative injuries. There was a significant (p < 0.01) increase in levels of ALT, AST, GGT, MDA, creatinine, and urea, and significant (p < 0.01) decrease in SOD, CAT, GPx, GST, GSH, and G6Pase of DF-intoxicated rats when compared with normal control. Pretreatment of DF-intoxicated rats with DGE significantly (p < 0.01) reduced the levels of ALT, AST, GGT, MDA, creatinine, and urea, and significantly (p < 0.01) elevated the levels of SOD, CAT, GPx, GST, GSH, and G6Pase compared with DF control. These findings showed that DGE may protect against DF-induced oxidative stress and hepatorenal injuries in rats.

Hypoglycemic and antioxidant activities of Caesalpinia ferrea Martius leaf extract in streptozotocin-induced diabetic rats

ObjectiveTo evaluate the antidiabetic and antioxidant effects of aqueous ethanolic extract of Caesalpinia ferrea (C. ferrea) leaf in normal and streptozotocin (STZ) induced diabetic rats. MethodsMale Sprague-Dawley rats divided into 6 groups of 6 rats each were assigned into diabetic and non-diabetic groups. Diabetes was induced in rats by single intraperitoneal administration of STZ (65 mg/kg body weight). C. ferrea extract at the doses of 250 and 500 mg/kg body weight was orally administered to both diabetic and non-diabetic animals for a period of 30 days. After completion of experimental duration serum, liver and pancreas were used for evaluating biochemical and histopathological changes. ResultsOral administration of C. ferrea leaf extract significantly reduced elevated serum glucose, α-amylase, liver function levels and significantly increased serum insulin, total protein and body weight as well as improved lipid profile due to diabetes. Furthermore, the treatment resulted in a marked increase in glutathione peroxidase, superoxide dismutase, catalase and reduced glutathione, and diminished levels of lipid peroxidation in liver and pancreas of diabetic rats. Histopathological studies demonstrated the reduction in the pancreas and liver damage and confirmed the biochemical findings. ConclusionsFrom the present study, it can be concluded that the C. ferrea leaf extract effectively improved hyperglycaemia while inhibiting the progression of oxidative stress in STZ-induced diabetic rats. Hence, it can be used in the management of diabetes mellitus.

Protective effect of Artemisia annua L. extract against galactose-induced oxidative stress in mice.

Artemisia annua L. (also called qinghao) has been well known as a source of antimalarial drug artemisinins. In addition, the herb was reported to have in vitro antioxidative activity. The present study investigated the protective effect of aqueous ethanol extract of Qinghao (AA extract) against D-galactose-induced oxidative stress in C57BL/6J mice. Feeding AA extract-containing diet lowered serum levels of malondialdehyde and 8-OH-dG that are biomarkers for lipid peroxidation and DNA damage, respectively. Furthermore, AA extract feeding enhanced the activity of NQO1, a typical antioxidant marker enzyme, in tissues such as kidney, stomach, small intestine, and large intestine. In conclusion, AA extract was found to have antioxidative activity in mouse model.

Protective potential of Tamarindus indica against gentamicin-induced nephrotoxicity

Context: Gentamicin is an antibiotic that is effective against Gram-negative microorganisms. However, its clinical applications are often limited due to nephrotoxic effects.Objective: This study investigated the protective effects of aqueous-ethanol extract of Tamarindus indica L. (Leguminosae) fruits against gentamicin-induced renal toxicity.Materials and methods: A daily dose of 200 mg/kg of 70% aqueous-ethanol extract derived from T. indica was employed in male rabbits as a co-therapy with gentamicin (80 mg/kg) for a period of three weeks. Serum and urinary renal function parameters and histological assessments were carried out and compared with one way analysis of variance (Graphpad prism version 5.00, Graphpad Software, San Diego, CA).Results: The results showed that gentamicin-treated animals had significantly elevated blood urea nitrogen (54.1 ± 2.6 mg/dl), serum creatinine (4.0 ± 0.1 mg/dl), serum uric acid (2.3 ± 0.1 mg/dl) and urinary protein excretion (3.8 ± 0.3 mg/dl) with a fall in body weight (10 ± 1%), creatinine clearance (0.7 ± 0.09 ml/min), serum potassium (3.4 ± 0.1 mEq/l), serum calcium (7.6 ± 0.2 mg/dl), urinary volume (126 ± 9 ml/24 h) and urinary lactate dehydrogenase secretion (103.1 ± 4.2 U/l). However, animals treated by co-therapy with gentamicin and T. indica had significantly improved renal structure and function.Discussion and conclusion: Co-therapy of 200 mg/kg/d of T. indica for a period of three weeks successfully prevented functional and morphological derangements caused by gentamicin as assessed by different renal function parameters and histological examinations.

Acute to Subchronic Toxicity and Reproductive Effects of Aqueous Ethanolic Extract of Rhizomes of Lasia spinosa Thw. in Male Rats

Acute to Subchronic Toxicity and Reproductive Effects of Aqueous Ethanolic Extract of Rhizomes of Lasia spinosa Thw. in Male Rats

Aqueous ethanolic extract of Cochlospermum planchonii rhizome enhances spermatogenesis in male albino rats

Cochlospermum planchonii has numerous therapeutic benefits and is widely used in folklore medicine of many African countries. This study was designed to investigate the effects of aqueous ethanolic extract of Cochlospermum planchonii root on sperm characteristics of albino rats. Animals were assigned into four groups comprising a control, which received only distilled water and treatment groups at the doses of 100, 200 and 400 mg/kg body weight daily for 30 days, respectively. The aqueous ethanolic extract of C. planchonii did significantly increase (P<0.05) the weights of the testes and accessory sex organs. Sperm count and sperm motility of rats given the extract increased (P<0.05) relative to the control. Percentages of abnormal sperm cells remained unchanged. The results of this study indicate that aqueous ethanolic extract of Cochlospermum planchonii increased spermatogenesis in the male rats. Keywords: Cochlospermum planchonii , sperm characteristics, reproduction, Wistar rats

Antihyperglycaemic and antihyperlipidaemic effects of aqueous ethanol extract of Tapinanthus globiferus leaves and Treculia africana root bark and their mixture on alloxan diabetic rats

Antihyperglycaemic and antihyperlipidaemic effects of aqueous ethanol extract of Tapinanthus globiferus leaves and Treculia africana root bark and their mixture on alloxan diabetic rats

Effect of aqueous-ethanolic extract of Nigella sativa on cisplatin-induced nephrotoxicity in rats

Effect of aqueous-ethanolic extract of Nigella sativa on cisplatin-induced nephrotoxicity in rats

Anti-Diabetic Effects of Aqueous Ethanolic Extract of Hibiscus rosasinensis L. on Streptozotocin-Induced Diabetic Rats and the Possible Morphologic Changes in the Liver and Kidney

Anti-Diabetic Effects of Aqueous Ethanolic Extract of Hibiscus rosasinensis L. on Streptozotocin-Induced Diabetic Rats and the Possible Morphologic Changes in the Liver and Kidney

Effect of Aqueous-Ethanolic Extract from Rosa damascena on Guinea Pig Isolated Heart

Objective(s) In the present study, the effects of aqueous-ethanolic extract from Rosa damascena on heart rate and contractility were examined. Materials and Methods Isolated guinea-pig hearts were perfused through aorta in a Langendorff mode. Heart rate (HR) and contractility were determined in the presence of four concentrations of the extract (0.1, 0.2, 0.4 and 1.0 mg %) and isoprenaline (1, 10, 100 nM and 1 µM) in comparison with baseline values in the presence and absence of propranolol (n= 10 for each group). Results Both isoprenaline and the extract caused increase in heart rate and contractility (P< 0.05 to P< 0.001). The percent increased in HR due to the final concentration of isoprenaline in the absence of propranolol was significantly greater than that of the extract (P< 0.01). Propranolol caused significant reduction in both HR and contractility (P< 0.05 for both) but this effect was significantly reversed by isoprenaline and the extract (P< 0.05 to P< 0.001). The percent increased in heart contractility due to the final concentration of the extract in the absence and presence of propranolol was significantly greater than that of isoprenaline (P< 0.05 for both cases). There was significant correlation between both HR and heart contractility with concentration of isoprenaline and the extract (P< 0.05 to P< 0.001). Conclusion In conclusion this study showed a relatively potent inotropic and chornotropic effect for Rosa damascena on isolated guinea-pig heart.

Ameliorating reactive oxygen species-induced in vitro lipid peroxidation in brain, liver, mitochondria and DNA damage by Zingiber officinale Roscoe.

Iron is an essential nutrient for a number of cellular activities. However, excess cellular iron can be toxic by producing reactive oxygen species (ROS) such as superoxide anion (O(2) (-)) and hydroxyl radical (HO(·)) that damage proteins, lipids and DNA. Mutagenic and genotoxic end products of lipid peroxidation can induce the decline of mitochondrial respiration and are associated with various human ailments including aging, neurodegenerative disorders, cancer etc. Zingiber officinale Roscoe (ginger) is a widely used spice around the world. The protective effect of aqueous ethanol extract of Z. officinale against ROS-induced in vitro lipid peroxidation and DNA damage was evaluated in this study. The lipid peroxidation was induced by hydroxyl radical generated from Fenton's reaction in rat liver and brain homogenates and mitochondrial fraction (isolated from rat liver). The DNA protection was evaluated using H(2)O(2)-induced changes in pBR-322 plasmid and Fenton reaction-induced DNA fragmentation in rat liver. The results indicated that Z. officinale significantly (P<0.001) protected the lipid peroxidation in all the tissue homogenate/mitochondria. The extract at 2 and 0.5 mg/ml could protect 92 % of the lipid peroxidation in brain homogenate and liver mitochondria respectively. The percent inhibition of lipid peroxidation at 1mg/ml of Z. officinale in the liver homogenate was 94 %. However, the extract could partially alleviate the DNA damage. The protective mechanism can be correlated to the radical scavenging property of Z. officinale. The results of the study suggest the possible nutraceutical role of Z. officinale against the oxidative stress induced human ailments.

The effect of aqueous ethanolic extract of Stachytarpheta cayennensis on the histology of the liver and fasting blood sugar of non-diabetic and diabetic wistar rats

The effect of aqueous ethanolic extract of Stachytarpheta cayennensis on the histology of the liver and fasting blood sugar of non-diabetic and diabetic wistar rats