Effect Of Aloe Vera Extract Research Articles

Synergetic Effects of Aloe Vera Extract with Trimethylglycine for Targeted Aquaporin 3 Regulation and Long-Term Skin Hydration.

Aquaporin 3 (AQP3) channels are tetrameric membrane-bound channels that facilitate the transport of water and other small solutes across cell membranes in the skin. Decreased AQP3 expression is associated with skin dryness, skin aging, psoriasis, and delayed wound healing. Thus, our study focused on a novel combination based on Aloe barbadensis leaf extract and trimethylglycine for targeted AQP3 regulation in skin keratinocytes and deep skin moisturization. Firstly, a dose-finding cytotoxicity assay of the selected substances was performed with a 2,5-diphenyl-2H-tetrazolium bromide (MTT) indicator on HaCaT cells. The substances' ability to increase the amount of AQP3 in keratinocytes was evaluated in a keratinocyte cell culture by means of ELISA. Additionally, the deep skin hydration effect was confirmed in clinical research with healthy volunteers. According to the results, the maximum tolerated doses providing viability at 70% (MTDs) values for Aloe barbadensis leaf extract and trimethylglycine were 24.50% and 39.00%, respectively. Following the research and development, a complex based on Aloe barbadensis leaf extract and trimethylglycine in a 1:1 mass ratio exhibited a good cytotoxicity profile, with an MTDs value of 37.90%. Furthermore, it was shown that the combination had a clear synergetic effect and significantly increased AQP3 by up to 380% compared to the negative control and glyceryl glucoside (p < 0.001). It was clinically confirmed that the developed shower gel containing Aloe barbadensis leaf extract and trimethylglycine safely improved skin hydration after one use and over 28 days. Thus, this novel plant-based combination has promising potential for AQP3 regulation in the skin epidermis and a role in the development of dermatological drugs for the treatment of skin xerosis and atopic-related conditions.

Effect of Aloe Vera Extract in Inhibit of Candida albicans on Cured Acrylic Resin Plates

Introduction: Removable dentures are either whole or partial. Fixed dentures are firmly affixed to the patient's teeth. Dentures reduce functional problems caused by tooth loss, but many individuals don't realize how crucial cleaning them is. Long-term denture use can hinder the tongue and saliva's cleaning of the dental mucosa. Thus, Candida albicans cling better to denture bases and primary agen in candidiasis oral. Aloe vera leaves contain six antimicrobial compounds that prevent the growth of various fungi, bacteria, and viruses, including Candida albicans and Staphylococcus pyogen. Objective: Knowing the effect of aloe vera extract (Aloe vera) with a concentration. Methods: The research method is True Experimental Laboratory, which uses a research design in post-test-only control groups. The Kruskal-Wallis test was used to analyze and interpret the research data results, and the Mann-Whitney U test was used as a follow-up analysis. Results: The normality test results showed a p-value of 0.033 in the control group of aquades smaller than 0.05. This proves that the control equates are generally not distributed because the p-value is smaller than 0.05 (p-value 0. The p-value 0.05 was obtained in the control group, proving that the control group is normally distributed. In the homogeneity test, a p-value of 0.000, smaller than 0.05, is obtained. This proves that the test data results in the control and treatment groups are not homogeneous. Conclus inhibits the development of Candida albicans in heat-cured acrylic resin.

Evaluation the Inhibitory Effect of Aloe Vera Aqueous and Alcoholic Extracts on Salivary Streptococcus Mutans In Comparison to Chlorohexidine Gluconate

diabetic properties. The aim of this study (an in vitro study) was to evaluate the effect of Aloe Vera alcoholic and water aqueous extract in different concentration (18%, 28%, 38%, and 48%) on the viable count and inhibition zone of streptococcus mutans and compared with chlorhexidine 2%. In this study the isolation and identification of streptococcus mutans was performed from saliva of individual, aged under 65 years old, these bacteria was isolated, purified and diagnosed according to morphological characteristic features and biochemical test. The effect of Aloe Vera extracts in different concentrations were tested on the isolated species by using agar diffusion technique and the effect of different concentrations of aloe vera on viable count of streptococcus mutans.Results: In vitro experiments, statistically reduction in the viable count of streptococcus mutans was recorded at all concentration of alcoholic Aloe Vera extract and increase in the concentration lead to increase in the reduction of viable count of streptococcus mutans while in this study showed significant reduction in viable count of streptococcus mutans at higher concentration of water extract of Aloe Vera at 38% and 48%.The diameter of inhibition zone of streptococcus mutans were highly significant at all concentration of alcoholic extract in comparison to water extract

Effect of Aloe vera extract on some parameter liver functions changes and complete blood count induced by Azathioprine in male rats

The present study aimed to reveal the biological vitality of the immune suppressant medicine azathioprine (AZA), which has been used to treat immunological illnesses like acute lymphoblastic leukemia (ALL), inflammatory bowel disease, and organ transplants, successfully lowers immune reactivity for liver and bone marrow. The goal of the current study was to evaluate the preventive effects of Aloe Vera extract in treating hematological and biochemical side effects brought on by azathioprine. Have been selected There were four groups of adult male rats. A mixture of azathioprine (50 mg/kg) and Aloe Vera (500 mg/kg) was given to group 4 in addition saline that group 1 received. Group 2 received azathioprine (50 mg/kg) orally for during a 30 days period. The findings show that azathioprine significantly reduced blood test results (RBC , WBC , PCV). While AST and ALT levels significantly increased in the biochemical test in contrast to the control group , Conclusions the Hepatotoxicity, or liver damage from immunosuppressive medications like azathioprine, is a possible side effect and Due to the damage that has happened to the hepatic tissue, the enzymes that assess the activities of the liver are raised.

The Effect of Aloe Vera Extract in Wound Healing of Buccal Mucosa in White Rats: An In Vivo Study

The Effect of Aloe Vera Extract in Wound Healing of Buccal Mucosa in White Rats: An In Vivo Study

Effect of aloe vera on the proliferation phase of oral mucosal wound healing in rats

Aloe vera is known as a medicinal plant containing an active substance acemannan thought to play a role in accelerating wound healing. This study aims to determine the effect of aloe vera extract on the proliferation phase of oral mucosa wound healing in rats by looking at epithelial thickening, epithelial gaps closure, growth of new blood vessels, and connective tissue growth. This research is a pure experimental study with a post-test only control group design. On the left buccal mucosa of the mouth of 24 white Wistar rats (n = 24), an incision was made by scalpel with 10 mm length and 2.5 mm depth. The rats were divided into 4 groups: groups 1 and 2 as controls, while groups 3 and 4 were applied with aloe vera extract made by maceration technique. Wound tissue was observed histologically with Hematoxylin-Eosin staining under a microscope with an image analyzer for measuring the thickness of epithelium. The analysis of epithelial gap width used morphometry, and the number of capillaries was counted manually. Connective tissue thickness was measured with Image-J software. These were carried out on days 7 and 14 after treatment and the data were analyzed with the independent t-test and Mann-Whitney test. The results showed that aloe vera extract had no significant effect on epithelial thickening on day 7 (p = 0.701) nor on day 14 (p = 0.639), but a significant effect was shown on epithelial gaps closing (p = 0.049), connective tissue thickening (p = 0.004), and capillary increase on day 7 (p = 0.049). It could be concluded that aloe vera extract could potentially accelerate the epithelial gaps closure, connective tissue thickening, and capillary increase during the proliferation phase of the oral mucosa wound healing.

Effect of Aloe Vera Extract and Second Line Anti-Tuberculosis Drugs on Mycobacterium Tuberculosis Strain-H37Rv

The present study was undertaken to examine the direct effect of second line anti-tuberculosis drugs Ethionamide (ETH), Para amino salicylic acid (PAS), Aloe vera on Mycobacterium tuberculosis (MTB) strain H37Rv ATCC No- 27294. It is found that Aloe vera does not interfere with single or in the combination of both ETH and PAS showing the bioenhancer activity. In vitro study of Aloe vera observed that the extract inhibited the growth of H37Rv strains. The present results will pave new avenues to find a new medicine that possesses Aloe vera alone or in combination with drugs to combat H37Rv strains controlling tuberculosis.

Effect of Aloe vera extract in post-burn skin repair in rats

Background: Burn injury is a global health problem that is most often caused by heat. A burn injury can cause high morbidity and mortality and requires high cost. Therefore, the use of plants as an herbal medicine has the potential to be developed in Indonesia. Aloe vera contains various active ingredients that help the wound healing process, such as glucomannan and acemannan which have the effects on the proliferation of macrophages, fibroblasts, and re-epithelialization. This study aimed to determine the effect of Aloe vera extract in repairing post-burn skin in rats that were analyzed from the number of macrophages, fibroblasts, and epidermal thickness. Methods: This is an experimental study with a posttest-only control group design using 54 Rattus norvegicus Wistar strain rats. The sampling method was simple random sampling consisting of 3 groups, i.e., I. standard group, which were normal rats; II. negative control group, which were given second-degree burns and treated with gel base (without Aloe vera extract); III. treatment groups, which were given second-degree burns and treated with Aloe vera extract gel. Each group was subdivided into three smaller groups (n = 6) according to the time the lesions were evaluated. Skin tissue samplings were carried out on days 3, 14, and 21 after injury to observe the number of macrophages, fibroblasts, and epidermal thickness. Results: There were significant differences in the mean number of macrophages, number of fibroblasts, and epidermal thickness in all groups (p&lt;0.05). Conclusion: Based on the result of the study, Aloe vera extract could fasten the healing process of 2nd degree burn wound in rats.

Effect of Aloe Vera Extract on Some Parameter Complete Blood Count and Liver Functions Induced by Azathioprine in Changes Male Rats

Effect of Aloe Vera Extract on Some Parameter Complete Blood Count and Liver Functions Induced by Azathioprine in Changes Male Rats

UJI EFEKTIFITAS Aloe vera TERHADAP PERTUMBUHAN Salmonella typhi SECARA IN VITRO

Aloe vera has many health benefits, containing saponins, aloin anthraquinone complex, barbaloin, isobarbaloin, athranol, aloemodin, cinnamic acid, chrysophanic acid, reistanol and essential oils. The aim of the study was to determine the effect of Aloe vera extract on the growth of Salmonella typhi bacteria. Research is experimental. Research Methods with Kirby Bouer. Concentrations were 100%, 70% and 50% with chloramphenicol control on Muller Hinton media. The results of the study based on the growth inhibition zone of Salmonella typhi with a minimum concentration of 50% have shown the ability to inhibit bacterial growth with the results of inhibition zones of each concentration of 50% of the inhibition zone being 7 ± 0.66 mm, 70% : 8.4 ± 0.38 and 100% : 12 ± 0.65. Inhibition zone of control : 22.58 ± 0.28. Aloe vera has the ability to inhibit the growth of Salmonella typhi in vitro

Effects of Aloe vera extract on growth, viability, ultrastructure and expression of mRNA for antioxidant enzymes in bovine secondary follicles cultured in vitro

This study aimed to investigate the effects of Aloe vera extract on follicular growth, viability, ultrastructure, and mRNA levels for superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase 1 (GPX1) and peroxiredoxin 6 (PRDX6) in bovine secondary follicles cultured in vitro. To this end, secondary follicles were mechanically isolated from the ovarian cortex and cultured at 38.5 °C, with 5% CO2 in air, for 18 days in TCM-199+ alone or supplemented with 2.5%, 5.0%, 10.0% and 20.0% Aloe vera extract. Follicular growth, morphology and antrum formation were evaluated every 6 days, while ultrastructure was evaluated at the end of culture. Analysis of viability was performed by calcein-AM and ethidium homodimer-1, while mRNA levels for SOD, CAT, GPX1 and PRDX6 were evaluated by real-time PCR at the end of culture. The results show that follicles cultured with 2.5% Aloe vera had increased the rate of antrum formation, while 2.5% and 5.0% Aloe vera improved follicular viability rate. Follicles cultured with 2.5% and 10.0% Aloe vera increased the levels of mRNA for SOD and GPX1 respectively, but the levels of CAT were reduced in follicles cultured with 2.5%, 5.0%, 10.0% and 20.0%. Additionally, follicles cultured with 2.5% of Aloe vera had their ultrastructure well preserved, while those cultured with 5.0%, 10.0% and 20.0% exhibited increased oocyte vacuolization and damaged organelles. In conclusion, 2.5% Aloe vera increases antrum formation, viability and expression of mRNA for SOD in cultured secondary follicles, but higher concentrations of Aloe vera have negative effects on follicular ultrastructure.

The Effect of Aloe Vera Extract on Blood Glucose Levels in Streptozotocin-Induced Rats

Aloevera can protect and restore the function of damaged pancreatic beta cells. The content of aloe vera can work like insulin, and lower blood glucose even though all beta cells have degenerated. The aim of the study was to find out the effect of aloe vera extract on the regulation of blood glucose levels in rats induced by streptozotocin. The study was true experimental randomized pre-post test control group design. The sampling technique used simple random sampling consisting of 20 wistar strain rats divided into 4 groups, namely the placebo control group, treatment group with a dose of 250 mg/dl, a dose of 350 mg/dl, and a dose of 1000 mg/dl. Rats were given STZ injection on day 8 after acclimatization. The independent variable was aloe vera extract. The dependent variable was blood glucose levels. The intervention of giving aloe vera extract was given for 9 days. Data were analyzed by Paired t Test and ANOVA. The results showed that there was an effect of aloe vera extract at a dose of 350 mg/kgBW on blood glucose levels with a paired t test of 0.022. The ANOVA results showed that aloe vera extract at a dose of 350 mg/kgBW had the highest effectiveness with a p value of 0.05. The chromium, alprogen, and flavonoid in aloe vera will improve the beta function of the pancreas in producing the hormone insulin. Damage to the islets of Langerhans cells in the pancreas will be inhibited and restore the sensitivity of the insulin receptor cells.methods that are attractive to high school students and carried out consistently.

Effect of Aloe vera extract in post-burn skin repair in rats.

Background: Burn injury is a global health problem that is most often caused by heat. A burn injury can cause high morbidity and mortality and requires high cost. Therefore, the use of plants as an herbal medicine has the potential to be developed in Indonesia. Aloe vera contains various active ingredients that help the wound healing process, such as glucomannan and acemannan which have the effects on the proliferation of macrophages, fibroblasts, and re-epithelialization. This study aimed to determine the effect of Aloe vera extract in repairing post-burn skin in rats that were analyzed from the number of macrophages, fibroblasts, and epidermal thickness. Methods: This is an experimental study with a posttest-only control group design using 54 Rattus norvegicus Wistar strain rats. The sampling method was simple random sampling consisting of 3 groups, i.e., I. standard group, which were normal rats; II. negative control group, which were given second-degree burns and treated with gel base (without Aloe vera extract); III. treatment groups, which were given second-degree burns and treated with Aloe vera extract gel. Each group was subdivided into three smaller groups (n = 6) according to the time the lesions were evaluated. Skin tissue samplings were carried out on days 3, 14, and 21 after injury to observe the number of macrophages, fibroblasts, and epidermal thickness. Results: There were significant differences in the mean number of macrophages, number of fibroblasts, and epidermal thickness in all groups ( p<0.05). Conclusion: Aloe vera extract could accelerate the healing process of burns in rats.

Effect of Aloe Vera Extract as Green Corrosion Inhibitor on Medium Carbon Steel in Sulphuric Acid Environment

Medium carbon steel is widely consumed by various industrial sectors due to its attractive set of mechanical properties and low cost, but it experiences deterioration when exposed to a corrosive environment. In the present study, Aloe Vera plant extract was studied as a green corrosion inhibitor for medium carbon steel in an acidic medium. The presence of inhibitive compounds in Aloe Vera plant extract was determined by FTIR. Moreover, the inhibition efficiency was determined through gravimetric analysis and electrochemical analysis. The results show that the Aloe Vera plant extract provided inhibition efficiency of more than 90% in both gravimetric and electrochemical analyses. Furthermore, the shift in polarization curves depicts that this plant extract is a mixed type inhibitor acting as an anodic and cathodic inhibitor. Overall, Aloe Vera plant extract provides excellent corrosion inhibition to medium carbon steel in the H2SO4 environment and can be used as a green corrosion inhibitor for mitigating internal corrosion of pipelines and storage tanks.

Effect of Aloe vera extract in post-burn skin repair in rats.

Background: Burn injury is a global health problem that is most often caused by heat. Burn injury can cause high morbidity and mortality and requires high cost. Therefore, the use of plants as herbal medicine has the potential to be developed in Indonesia. Aloe vera contains various active ingredients that help the wound healing process, such as glucomannan and acemannan which have the effects on the proliferation of macrophages, and fibroblasts, and re-epithelialization. This study aimed to determine the effect of Aloe vera extract in repairing post-burn skin in rats that was analyzed from the number of macrophages and fibroblasts, and epidermal thickness. Methods: This is an experimental study with a posttest-only control group design using 54 Rattus norvegicus Wistar strain rats. The sampling method was simple random sampling consisting of 3 groups, i.e., I. standard group, which were normal rats; II. negative control group, which were given second-degree burns and treated with gel base (without Aloe vera extract); III. treatment groups, which were given second-degree burns and treated with Aloe vera extract gel. Each group was subdivided into three smaller groups (n = 6) according to the time the lesions were evaluated. Skin tissue samplings were carried out on day 3, 14, and 21 after injury to observe the number of macrophages and fibroblasts, and epidermal thickness. Results: There were significant differences in the mean number of macrophages, number of fibroblasts, and epidermal thickness in all groups ( p<0.05). Conclusion: Aloe vera extract gel could accelerate the healing process of burns in rats.

In vitro anti-obesity effect of Aloe vera extract through transcription factors and lipolysis-associated genes

In vitro anti-obesity effect of Aloe vera extract through transcription factors and lipolysis-associated genes

Anti-inflammatory Effect of Aloe vera Extract on Inflammatory Cytokines of Rats Fed with a High-Fat Diet (HFD)

Background: Cytokines are glycoprotein compounds with an important role in inducing and regulating inflammation. Objectives: The present study aimed to measure the effect of Aloe vera alcoholic extracts on inflammatory cytokines in rats fed with a high-fat diet. Methods: Forty adult male Wistar rats were purchased and randomly categorized into five groups, including two control groups (control and control fed a high-fat diet (HFD) and three experimental groups (high-fat diet + 150 mg/kg Aloe vera, high fat diet + 300 mg/kg Aloe vera, and high-fat diet + 600 mg/kg Aloe vera). The rats in the experimental groups received high-fat emulsion and three doses of Aloe vera for 60 days in the form of gavage. Following blood sampling, serum concentrations of tumor necrosis factor-alpha (TNF-α), transforming growth factor-beta (TGF-β), interleukin 6 (IL-6), and interferon-γ (INF-γ) were measured. Statistical analysis was administered using SPSS-20 software by ANOVA test. Mean comparisons were conducted via Duncan’s multiple range test at the 0.05 level of significance. Results: The findings showed that a high-fat diet (HFD) could increase the concentrations of inflammatory factors TNF-α and TGF-β (P &lt; 0.05). Moreover, an increase in the concentration of inflammatory factor IL-6 was observed at P &lt; 0.01. No significant effects were observed in the mean concentration of INF-γ in the study groups. The use of Aloe vera gel extract considerably reduced inflammatory factors TNF-α, TGF-β, and IL-6 in the Aloe vera extract-receiving groups. Conclusions: In general, the results of the present study revealed that Aloe vera alcoholic extract reduced inflammatory factors in the rats fed with a high-fat diet.

Antifungal Effect of Aloe Vera Extract on Otomycosis: A Review

Otomycosis is a common disease that can cause a variety of complications, including involvement of the inner ear and, in rare cases, death. Otomycosis management can be difficult and requires close monitoring. There is currently no standardized treatment regimen for otomycosis, which opens up new treatment options such as the use of herbal medicine. Aloe barbadensis miller, also known as Aloe vera, is one of over 400 Aloe vera species and a member of the Liliaceae family. Several studies have shown that Aloe vera extract has antifungal properties. The purpose of this literature review is to identify the components of Aloe vera as well as the mechanism of action in the treatment of otomycosis. The articles chosen were all published in PubMed within the last six years. The review included 7 articles that were obtained.

Aloe vera increases mRNA expression of antioxidant enzymes in cryopreserved bovine ovarian tissue and promotes follicular growth and survival after in vitro culture

The aims of the present study were to evaluate the effects of Aloe vera extract on expression of mRNA for antioxidant enzymes in bovine ovarian tissue after vitrification, as well as on follicular morphology, viability, activation and extracellular matrix in cultured ovarian tissues that had been previously vitrified. Fragments from bovine ovarian cortical tissue were cryopreserved in a vitrification solution alone or supplemented with two concentrations of Aloe vera (10 or 50%). After thawing, the cryopreserved tissues were analyzed by histological techniques, as well as the levels of mRNA for SOD, CAT, PRDX6 and GPX1 were investigated. Furthermore, cryopreserved fragments were then culture in vitro in α-MEM for 6 days. Histological evaluation of cultured tissues was performed to determine the percentages of normal and developing follicles. The results showed that, after vitrification, the presence of Aloe vera in both concentrations was able to maintain percentages of collagen fibers similar to fresh tissues (P < 0.05). Aloe vera in both concentrations significantly increased mRNA levels for PRDX6 and GPX1 in cryopreserved tissues, while 10% Aloe vera increased mRNA levels for SOD (P < 0.05). In parallel, after in vitro culture, fragments vitrified in the presence of 10% Aloe vera had significantly higher levels of morphologically healthy follicles when compared to tissue that were vitrified without Aloe vera. In fragments vitrified with Aloe vera, the rate of developing follicles was significantly higher than in tissues vitrified without Aloe vera. Tissues vitrified with 10% Aloe vera and cultured in vitro maintained percentages of collagen fibers similar to fresh tissues. In conclusion, 10% Aloe vera increases the expression of mRNA for PRDX6, GPX1 and SOD in vitrified ovarian tissues, maintains follicular survival and promotes activation and development of follicles after in vitro culture of vitrified bovine ovarian tissue.

Performance of Quails with Aloe vera Extract and Acid cheese whey Supplementation

A study was conducted to evaluate the effects of Aloe vera extract and Acid cheese whey on the growth and productivity of quails. A total of 300 quails distributed to 5 treatments with four replications using Complete Randomized Design (CRD). This was a four-month study starting from growing to laying period of quails. Treatment Levels were; T1= 0mL AVE and ACW, T2=15mL AVE, T3=25mL AVE, T4=15mL ACW, and T5=25mL ACW per gallon of water. Results revealed no significant differences on final weight, gain weight, feed consumption, and water consumption except on feed conversion ratio which is significantly different among treatments. Numerically, birds with AVE and ACW supplementation showed higher performance on the parameters studied. Results suggests that Aloe vera extract and Acid Cheese Whey can be used to increase growth and egg production performance in quails. KEY WORDS: T. gigantea leaf meal, Aloe extract, acid cheese whey, quail, egg qualities