Effects Of AGEs Research Articles

Advanced glycation end products impair bone marrow mesenchymal stem cells osteogenesis in periodontitis with diabetes via FTO-mediated N6-methyladenosine modification of sclerostin

BackgroundDiabetes mellitus (DM) and periodontitis are two prevalent diseases with mutual influence. Accumulation of advanced glycation end products (AGEs) in hyperglycemia may impair cell function and worsen periodontal conditions. N6-methyladenosine (m6A) is an important post-transcriptional modification in RNAs that regulates cell fate determinant and progression of diseases. However, whether m6A methylation participates in the process of periodontitis with diabetes is unclear. Thus, we aimed to investigate the effects of AGEs on bone marrow mesenchymal stem cells (BMSCs), elucidate the m6A modification mechanism in diabetes-associated periodontitis.MethodsPeriodontitis with diabetes were established by high-fat diet/streptozotocin injection and silk ligation. M6A modifications in alveolar bone were demonstrated by RNA immunoprecipitation sequence. BMSCs treated with AGEs, fat mass and obesity associated (FTO) protein knockdown and sclerostin (SOST) interference were evaluated by quantitative polymerase chain reaction, western blot, immunofluorescence, alkaline phosphatase and Alizarin red S staining.ResultsDiabetes damaged alveolar bone regeneration was validated in vivo. In vitro experiments showed AGEs inhibited BMSCs osteogenesis and influenced the FTO expression and m6A level in total RNA. FTO knockdown increased the m6A levels and reversed the AGE-induced inhibition of BMSCs differentiation. Mechanically, FTO regulated m6A modification on SOST transcripts, and AGEs affected the binding of FTO to SOST transcripts. FTO knockdown accelerated the degradation of SOST mRNA in presence of AGEs. Interference with SOST expression in AGE-treated BMSCs partially rescued the osteogenesis by activating Wnt Signaling.ConclusionsAGEs impaired BMSCs osteogenesis by regulating SOST in an m6A-dependent manner, presenting a promising method for bone regeneration treatment of periodontitis with diabetes.

Different modulation of STING/TBK1/IRF3 signaling by advanced glycation end products

Advanced glycation end products (AGEs) are a heterogeneous group of compounds that are non-enzymatically produced by reactions between carbonyl compounds and proteins. Many types of AGEs are produced according to the type or concentration of the reacting carbonyl compound. We have previously demonstrated that a glycolaldehyde-derived AGE suppresses stimulator of interferon gene (STING)/TANK-binding kinase 1 (TBK1)/interferon regulatory transcription factor 3 (IRF3), which is a component of the innate immune system. In this report, we investigated the effects of AGEs prepared by several carbonyl compounds on STING/TBK1/IRF3 signaling. AGEs used in the present study were numbered based on the carbonyl compound type: AGE1, derived from glucose; AGE2, derived from glyceraldehyde; AGE3, derived from glycolaldehyde; AGE4, derived from methylglyoxal; and AGE5, derived from glyoxal. AGEs derived from aldehyde (AGE2 and AGE3) and dicarbonyl compounds (AGE4 and AGE5) suppressed cyclic GMP-AMP (cGAMP)-induced activation of STING/TBK1/IRF3 signaling, with different suppression efficiencies observed. Lysine modification by carbonyl compounds was related to the efficiency of the suppressive effect on STING/TBK1/IRF3 signaling. Among the AGEs used, only AGE1 enhanced cGAMP-induced activation of STING/TBK1/IRF3 signaling. Enhancing the modulation of STING/TBK1/IRF3 signaling by AGE1 was mediated by toll-like receptor 4. These results indicated that modulation of STING/TBK1/IRF3 signaling by prepared AGEs is dependent on the type and concentration of the carbonyl compound present. Modulating STING/TBK1/IRF3 signaling by AGEs may involve modification of lysine residues in proteins.

Experimental and numerical study on the degradation law of mechanical properties of stress-corrosion steel wire for bridge cables

In this paper, the stress corrosion and monotonic tensile test of corroded steel wire were carried out. The effects of different corrosion ages and stress levels on failure modes, stress-strain curve and mechanical properties of corroded steel wire were discussed. Then, a finite element model of steel wire considering real surface morphology was established, and the surface stress distribution and the failure of steel wire under different corrosion degrees were compared. The relationship between stress triaxiality and critical equivalent plastic strain to different corrosion parameters was given. Finally, the degradation model of mechanical properties of corroded steel wires was established. The results showed that the failure patterns of steel wires with different degrees of corrosion were mainly divided into three types: cup cone shape, milling cutter shape and splitting shape. The shear lip zone of fracture section basically disappeared with the increase of stress corrosion, and the location of fiber area was transferred from the inside to the surface of steel wire. The decrease in strength and elastic modulus of the specimen under stress corrosion was four times and three times greater than that under non-stress corrosion, respectively. In addition, the crack position of specimen changed from the inside of section to the location of corrosion pit with the increase of corrosion degree. The fracture equivalent plastic strain decreased gradually, while the stress triaxiality increased. When the degree of corrosion reached 19.53%, the fracture equivalent plastic strain decreased by 55.41% from 0.314 to 0.140, and the stress triaxiality increased by 23.53% from 0.390 to 0.510. The influence of uniform thickness loss on the stress triaxiality was less than that of pit depth-width ratio. The larger the depth-width ratio, the larger the maximum stress triaxiality corresponding to the failure element. The constitutive model proposed in this paper could reflect the degradation law of mechanical properties of corroded steel wires.

Effect of ages on yield and quality of essential oil of betel leaf (Piper betle L.): Antioxidant activity, GC-MS and SEM analysis

In the present study, essential oil was extracted from the different ages (15, 30, 45 and 60 days) of betel leaves and evaluated their yield and quality of the essential oil. The effect of ages on percentage yield, chemical composition, morphological and physical properties, as well as antioxidant activity of essential oil was assessed and compared. The essential oil yield content of various ages of betel leaves, such as 15, 30, 45 and 60 days, is found to be 0.65%, 0.60%, 0.50% and 0.45%, respectively. Despite this, chemical compositions of extracted essential oil from various ages were analyzed by GC-MS analysis, and it was found that a total number of 55–65 volatile bioactive compounds are present, accounting for 97.78–98.88% of the extracted essential oil composition. The major bioactive compounds found in the all-ages of betel leaf essential oil are indanol (13.51–24.70%), elemene (4.36–10.84%) and eugenol (41.14%). Furthermore, 60 days old betel leaves essential oil exhibited higher antioxidant activity (87.01%) as compared to the 15 days younger betel leaves (84.05%). Moreover, SEM analysis was used to conduct a morphological investigation to examine the extraction mechanism of different stages of betel leaves. Therefore, obtained bioactive compounds from different ages of betel leaf essential oil can be used for various biological activities, food and pharmaceutical industries. In light of the findings, it is recommended that early-stage or younger leaves release higher essential oil while older leaves possess excellent antioxidant activity.

Advanced glycation end products induce nucleus pulposus cell apoptosis by upregulating TXNIP via inhibiting glycolysis pathway in intervertebral disc degeneration.

Accumulation of advanced glycation end products (AGEs) causes apoptosis in human nucleus pulposus cells (NPCs), contributing to intervertebral disc degeneration (IVDD). The purpose of this study was to determine the roles of thioredoxin-interacting protein (TXNIP) in the mechanisms underlying AGE-induced apoptosis of NPCs. TXNIP was silenced or overexpressed in HNPCs exposed to AGEs. Glycolysis was assessed using extracellular acidification rate (ECAR), ATP level, GLUT1, and GLUT4 measurements. AGEs, TXNIP, GLUT1, and GLUT4 levels in IVDD patients were measured as well. In NPCs, AGEs reduced cell viability, induced apoptosis, inhibited glycolysis, and increased TXNIP expression. Silencing TXNIP compromised the effects of AGEs on cell viability, apoptosis, and glycolysis in NPCs. Furthermore, TXNIP overexpression resulted in decreased cell viability, increased apoptotic cells, and glycolysis suppression. Furthermore, co-treatment with a glycolysis inhibitor improved TXNIP silencing's suppressive effects on AGE-induced cell injury in NPCs. In IVDD patients with Pfirrmann Grades II-V, increasing trends in AGEs and TXNIP were observed, while decreasing trends in GLUT1 and GLUT4. AGE levels had positive correlations with TXNIP levels. Both AGE and TXNIP levels correlated negatively with GLUT1 and GLUT4. Our study indicates that TXNIP plays a role in mediating AGE-induced cell injury through suppressing glycolysis. The accumulation of AGEs, the upregulation of TXNIP, and the downregulation of GLUT1 and GLUT4 are all linked to the progression of IVDD.

AGEs promote atherosclerosis by increasing LDL transcytosis across endothelial cells via RAGE/NF-κB/Caveolin-1 pathway

ObjectiveTo elucidate the mechanism whereby advanced glycation end products (AGEs) accelerate atherosclerosis (AS) and to explore novel therapeutic strategies for atherosclerotic cardiovascular disease.Methods and resultsThe effect of AGEs on low-density lipoprotein (LDL) transcytosis across endothelial cells (ECs) was assessed using an in vitro model of LDL transcytosis. We observed that AGEs activated the receptor for advanced glycation end products (RAGE) on the surface of ECs and consequently upregulated Caveolin-1, which in turn increased caveolae-mediated LDL transcytosis and accelerated AS progression. Our molecular assessment revealed that AGEs activate the RAGE-NF-κB signaling, which then recruits the NF-κB subunit p65 to the RAGE promoter and consequently enhances RAGE transcription, thereby forming a positive feedback loop between the NF-κB signaling and RAGE expression. Increased NF-κB signaling ultimately upregulated Caveolin-1, promoting LDL transcytosis, and inhibition of RAGE suppressed AGE-induced LDL transcytosis. In ApoE−/− mice on a high-fat diet, atherosclerotic plaque formation was accelerated by AGEs but suppressed by EC-specific knockdown of RAGE.ConclusionAGEs accelerate the development of diabetes-related AS by increasing the LDL transcytosis in ECs through the activation of the RAGE/NF-κB/Caveolin-1 axis, which may be targeted to prevent or treat diabetic macrovascular complications.

Cathepsin D Attenuates the Proliferation of Vascular Smooth Muscle Cells Induced by the AGE/RAGE Pathway by Suppressing the ERK Signal.

In this study, we aimed to clarify the role and mechanism by which Cathepsin D (CTSD) mediates the advanced glycation end products (AGEs)-induced proliferation of vascular smooth muscle cells (VSMCs). We conducted a Western blotting assay and co-immunoprecipitation assay to detect the expression of target proteins and the interaction between different proteins. Cell Counting Kit-8 (CCK-8) assay and 5- ethynyl-2'-deoxyuridine (EdU) were used to evaluate the proliferation. AGEs significantly promoted phenotypic switching and proliferation of VSMCs in a concentration-dependent manner. This effect of AGEs was accompanied by inhibition of CTSD. Both the proliferation of VSMCs and inhibition of CTSD induced by AGEs could be attenuated by the specific inhibitor of the receptor for advanced glycation end products (RAGE), FPS-ZM1. Overexpression of CTSD significantly alleviated these effects of AGEs on VSMCs. The mechanism of CTSD action in VSMCs was also explored. Overexpression of CTSD reduced the activation of p-ERK caused by AGEs. By contrast, the knockdown of CTSD, elicited using a plasmid containing short hairpin RNA (shRNA) against CTSD, further increased the activation of p-ERK compared to AGEs alone. Additionally, co-immunoprecipitation studies revealed an endogenous interaction between CTSD, a protease, and p-ERK, its potential substrate. It has been demonstrated that CTSD downregulates the level of phosphorylated ERK by degrading its target, and this interaction plays a critical role in the proliferation of VSMCs induced by the AGE/RAGE axis. These results provide a novel insight into the prevention and treatment of vascular complications in diabetes.

Electroencephalographic features of the developing brain in 72 dogs under xylazine sedation: a visual and statistical analysis.

Electroencephalogram (EEG) is a neurophysiological test, which is widely used in human medicine for epilepsy diagnosis and other neurological disorders. For an adequate interpretation, it is necessary to know the electroencephalogram features for different stages of development. Despite the growing interest in its implementation in veterinary medicine, standardized descriptions of the EEG features of the different stages of brain development in dogs are restricted to studies with limited number of dogs and limited age groups. In this research, the electroencephalographic recording of 72 dogs of different breeds and ages was carried out under xylazine sedation to determine tracing characteristics by visual analysis and through statistical analysis of power spectrum. To establish the EEG features of recordings, 3 essential aspects were selected: (a) the presence or absence of slow waves of 4 to 6-7 Hz; (b) the comparison of the electrical activity recorded in the temporal and dorsal cortex channels; and (c) the visual increase of the alpha activity. Visual analysis on both reference and bipolar montage was performed by the authors and additionally blindly corroborated by two human neurophysiologists. The results allowed us to differentiate 5 age groups: 0-5, 6-11, 12-17, 18-23, and >24 months. Statistical analysis of the power spectrum was performed by analysis of variance (ANOVA) with a completely randomized design (CRD) under factorial arrangement by observing the effect of ages, channels and electroencephalographic rhythms on relative power. The results obtained matched those observed in the visual analysis. According to our results, the characteristics of the EEG corresponding to the adult animal begin to appear at 12 months of age but stabilize after 24 months of age. In this case, the evident differences in the processes of development and maturation of the neopallium and the rhinencephalon play a determining role. Our results differ from those obtained by other authors, probably due to the addition of a deep electrode that facilitates the recording of temporal cortical activity and its deeper rhinencephalic connections.

Global, regional, and national burden of asthma and its attributable risk factors from 1990 to 2019: a systematic analysis for the Global Burden of Disease Study 2019

BackgroundThe burden of asthma in terms of premature death or reduced quality of life remains a huge issue. It is of great importance to evaluate asthma burden geographically and time trends from 1990 to 2019 and to assess the contributions of age, period, and cohort effects at global level.MethodsAsthma prevalence, deaths, and disability adjusted life years (DALYs) as well as risk-attributable burden were collected from the Global Burden of Diseases, Injuries, and Risk Factors Study (GBD) 2019 database and were compared by age and sex. The Smoothing Splines models were used to estimate the relationship between asthma DALYs and the sociodemographic index (SDI). The Age-Period-Cohort model was used to determine effects of ages, periods, and birth cohorts on disease rates.ResultsBetween 1990 and 2019, the declines were 24.05% (95% uncertainty interval [UI] − 27.24 to − 20.82) in age-standardized asthma prevalence, 51.3% (− 59.08 to − 43.71) in mortality, and 42.55% (− 48.48 to − 36.61) in DALYs rate. However, the burden of asthma continued to rise, with an estimated 262.41 million prevalent cases globally (95% UI 224.05 to 309.45). Asthma caused greater DALYs in females than in males among people aged 20 years and older. The lowest age-standardized DALYs rate was observed at a SDI of approximately 0.70. The Longitudinal age curves showed an approximate W-shaped pattern for asthma prevalence and a likely J-shaped pattern for asthma mortality. The period effect on prevalence and mortality of asthma decreased from 1990 to 2019. Compared with the 1955–1959 birth cohort, the prevalence relative risk (RR) of asthma was highest in the 1905–1909 birth cohort, whereas the mortality RR continued to decline. At the global level, the percentages of high body-mass index, occupational asthmagens, and smoking contributing to DALYs due to asthma were 16.94%, 8.82%, and 9.87%, respectively.ConclusionsAlthough the age-standardized rates of asthma burden declined in the past 30 years, the overall burden of asthma remains severe. High body mass index becomes the most important risk factor for DALYs due to asthma at the global level.

Advanced glycation end products promote the progression of chronic kidney diseases by targeting calpain 6.

Advanced glycation end products (AGEs) are produced by glycosylation or oxidation of proteins and lipids and are tightly involved in the chronic kidney disease (CKD) process. Calpain 6 (CAPN6) is a non-classical calpain that has been reported to be overexpressed in CKD. This study aimed to explore the effects of AGEs in CKD progress and their correlation with CAPN6. AGEs production was measured using ELISA. The CCK-8 assay was used to test cell proliferation. mRNA and protein levels were tested using qRT-PCR and western blot. The progress of glycolysis was tested by calculating the ATP and ECAR content in HK-2 cells. The expression of AGEs and CAPN6 was significantly increased in patients with CKD3, CKD4, and CKD5. AGEs treatment inhibited cell proliferation and glycolysis and accelerated apoptosis. Additionally, CAPN6 knockdown effectively reversed the effects of AGEs in HK-2 cells. In addition, overexpressed CAPN6 played similar role to AGEs, which suppressed cell proliferation and glycolysis and facilitated apoptosis. Moreover, the administration of 2-DG, a glycolysis inhibitor, counteracted the effects of CAPN6 silencing in HK-2 cells. Mechanistically, CAPN6 interacts with NF-κB and PDTC reduced CAPN6 expression in HK-2 cells. This investigation revealed that AGEs facilitate CKD development in vitro by modulating the expression of CAPN6.

Pharmacoepidemiologic study of association between apparent treatment resistant hypertension, cardiovascular disease and interaction effect by sex and age

A limited number of studies have been conducted to test the magnitudes of the association between apparent treatment resistant hypertension (aTRH) and risk of cardiovascular disease (CVD). To investigate the association between aTRH and risk of CVD and examine whether sex and age modify this association. We applied an observational analysis study design using data from the United States Antihypertensive and Lipid-Lowering Treatment to Prevent Heart Attack Trial (ALLHAT). ALLHAT recruited participants (n = 25516) from 625 primary care settings throughout the United States, Canada, Puerto Rico, and United States Virgin Islands, aged 55 and older with hypertension and at least one additional risk factor for heart disease. aTRH was assessed from the year 2 visit. CVD event was defined as one of the following from the year 2 follow-up visit: Fatal or non-fatal myocardial infarction, coronary revascularization, angina, stroke, heart failure, or peripheral artery disease. Cox proportional hazards regression was used to examine the effect of aTRH on CVD risk. Potential modifications of sex and age on this association were examined on the multiplicative scale by interaction term and additive scale by joint effects and relative excess risk for interaction. Of the total study participants (n = 25516), 5030 experienced a CVD event during a mean of 4.7 years follow-up. aTRH was associated with a 30% increase in risk of CVD compared to non-aTRH [hazards ratio (HR) = 1.3, 95%CI: 1.19-1.42]. Sex and age modified this relationship on both multiplicative and additive scales independently. Stratified by sex, aTRH was associated with a 64% increase in risk of CVD (HR = 1.64, 95%CI: 1.43-1.88) in women, and a 13% increase in risk of CVD (HR = 1.13, 95%CI: 1.01-1.27) in men. Stratified by age, aTRH had a stronger impact on the risk of CVD in participants aged < 65 (HR = 1.53, 95%CI: 1.32-1.77) than it did in those aged ≥ 65 (HR = 1.18, 95%CI: 1.05-1.32). Significant two-way interactions of sex and aTRH, and age and aTRH on risk of CVD were observed (P < 0.05). The observed joint effect of aTRH and ages ≥ 65 years (HR = 1.85, 95%CI: 1.22-2.48) in males was less than what was expected for both additive and multiplicative models (HR = 4.10, 95%CI: 3.63-4.57 and 4.88, 95%CI: 3.66-6.31), although three-way interaction of sex, age, and aTRH on the risk of CVD and coronary heart disease did not reach a statistical significance (P > 0.05). aTRH was significantly associated with an increased risk of CVD and this association was modified by both sex and age. Further studies are warranted to test these mechanisms.

Advanced glycation end products inhibit the osteogenic differentiation potential of adipose-derived stem cells in mice through autophagy

BackgroundDiabetes mellitus (DM) microenvironment will accelerate the accumulation of Advanced glycation end products (AGEs), adipose-derived stem cells (ASCs) have poor osteogenesis in the DM microenvironment. Studies suggest autophagy plays a vital role in osteogenesis, but the mechanism of the altered osteogenic potential of ASCs has not been elucidated. Bone tissue engineering by ASCs is widely used in the treatment of bone defects with diabetic osteoporosis (DOP). Therefore, it is meaningful to explore the effect of AGEs on the osteogenic differentiation potential of ASCs and its potential mechanism for the repair of bone defects in DOP. Materials and methodsASCs in C57BL/6 mice were isolated, cultured, then treated with AGEs, subsequently, cell viability and proliferation were detected through Cell Counting Kit 8 assay. 3-Methyladenine (3-MA), an autophagic inhibitor used to inhibit autophagic levels. Rapamycin (Rapa), an autophagy activator that further activated autophagy levels by inhibiting mTOR.The osteogenesis and autophagy changes of ASCs were analyzed by flow cytometry, qPCR, western blot, immunofluorescence, alkaline phosphatase (ALP) and alizarin red staining. ResultsAGEs reduced the autophagy level and osteogenic potential of ASCs. After 3-MA reduced autophagy, the osteogenic potential of ASCs also decreased. AGEs co-treatment with 3-MA, the levels of osteogenesis and autophagy reduced more significantly. When autophagy was activated by Rapa, it was found that it could rescue the reduced osteogenic potential of AGEs. ConclusionsAGEs reduce the osteogenic differentiation potential of ASCs through autophagy, and may provide a reference for the treatment of bone defects with diabetes osteoporosis.

Effects of Different Donor Ages on the Growth of Cutting Seedlings Propagated from Ancient Platycladus orientalis.

The effects of tree age on the growth of cutting seedlings propagated from ancient trees have been an important issue in plant breeding and cultivation. In order to understand seedling growth and stress resistance stability, phenotypic measurements, physiological assays, and high-throughput transcriptome sequencing were performed on sown seedlings propagated from 5-year-old donors and cutting seedlings propagated from 5-, 300-, and 700-year-old Platycladus orientalis donors. In this study, the growth of cutting seedlings propagated from ancient trees was significantly slower; the soluble sugar and chlorophyll contents gradually decreased with the increase in the age of donors, and the flavonoid and total phenolic contents of sown seedlings were higher than those of cutting seedlings. Enrichment analysis of differential genes showed that plant hormone signal transduction, the plant-pathogen interaction, and the flavone and flavonol biosynthesis pathways were significantly up-regulated with the increasing age of cutting seedlings propagated from 300- and 700-year-old donors. A total of 104,764 differentially expressed genes were calculated using weighted gene co-expression network analysis, and 8 gene modules were obtained. Further, 10 hub genes in the blue module were identified, which revealed that the expression levels of JAZ, FLS, RPM1/RPS3, CML, and RPS2 increased with the increase in tree age. The results demonstrated that the age of the donors seriously affected the growth of P. orientalis cutting seedlings and that cutting propagation can preserve the resistance of ancient trees. The results of this study provide important insights into the effects of age on asexually propagated seedlings, reveal potential molecular mechanisms, and contribute to an improvement in the level of breeding and conservation of ancient germplasm resources of P. orientalis trees.

Effects of Donor Ages and Propagation Methods on Seedling Growth of Platycladus orientalis (L.) Franco in Winter.

To evaluate the effects of donor ages on growth and stress resistance of 6-year-old seedlings propagated from 5-, 2000-, and 3000-year-old Platycladus orientalis donors with grafting, cutting, and seed sowing, growth indicators and physiological and transcriptomic analyses were performed in 6-year-old seedlings in winter. Results showed that basal stem diameters and plant heights of seedlings of the three propagation methods decreased with the age of the donors, and the sown seedlings were the thickest and tallest. The contents of soluble sugar, chlorophyll, and free fatty acid in apical leaves of the three propagation methods were negatively correlated with donor ages in winter, while the opposite was true for flavonoid and total phenolic. The contents of flavonoid, total phenolic, and free fatty acid in cutting seedlings were highest in the seedlings propagated in the three methods in winter. KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis of differentially expressed genes showed phenylpropanoid biosynthesis and fatty acid metabolism pathways, and their expression levels were up-regulated in apical leaves from 6-year-old seedlings propagated from 3000-year-old P. orientalis donors. In addition, hub genes analysis presented that C4H, OMT1, CCR2, PAL, PRX52, ACP1, AtPDAT2, and FAD3 were up-regulated in cutting seedlings, and the gene expression levels decreased in seedlings propagated from 2000- and 3000-year-old donors. These findings demonstrate the resistance stability of cuttings of P. orientalis and provide insights into the regulatory mechanisms of seedlings of P. orientalis propagated from donors at different ages in different propagation methods against low-temperature stress.

Effects of Host and Parasitoid Ages on Parasitism Efficiency of Dirhinus giffardii (Silvestri)1 against Zeugodacus cucurbitae2

Pesticides threaten the environment and increase amounts of resistance by pests. Ecologically friendly biological control of pests is being considered in scientific and political spheres. Fruit flies cause huge economic losses to more than 81 fruit and vegetable species. They are major pests in tropical and subtropical areas. This study used pupae of melon fly, Zeugodacus cucurbitae (Coquillett), as a host and Dirhinus giffardii (Silvestri) as the parasitoid, the biological control agent. During host-parasitoid interaction at different conditions of exposure, the rate of parasitism of a host by D. giffardii differed significantly in time. Least parasitism was of 1-day-old pupae (13.41 ± 1.10) exposed for 6 hours. Greatest percentage of parasitism was to 3-day-old pupae (43.5 ± 1.12) exposed for 24 hours. Few pupae younger than 3 days old were parasitized. Parasitoid emergence rate from pupae increased from 6 hours (21.49 ± 1.46), to 12 hours (28.30 ± 2.00), and 24 hours (33.93 ± 0.66). Percentage of emergence of 3-day-old pupae was 28.40 ± 0.59 at 6 hours, 29.51 ± 0.45 at 12 hours, and 34.11 ± 0.224 at 24 hours. To maximize in vivo production of parasitoids, 5-day-old female D. giffardii should be provided pupae ≤3 days old. Results contribute to further understanding host-parasitoid interaction important for sustainable and integrated pest management practices throughout the world.

Evaluation of Cancer disease for the period (1995-2005) in Tikrit teaching hospital

In this study we evaluate the 20 type of cancer disease in Tikrit teaching hospital for the period from 1995 to 2005 by using three statistical methods (regression analysis , T-test and completely random design) .The presentation and description of all cases among male and female were confirmed. And also explain the effect of ages for each type of cancer distribution among age groups of both sexes.The results of this study are (1)- The female cancers are leukemia, lung, bladder, uterus, breast, brain, stomach, pancreas, rectum, kidney, liver, urinary, larynx, thyroid gland, colon, bone, small intestine and skin (2)- The male cancers are leukemia, lung, bladder, prostate, bone, brain, stomach, pancreas, rectum, kidney, liver, urinary, larynx, thyroid gland, colon, lymphoma, small intestine and skin .(3)- There are significant different between male and female for 9 type of cancer namely , leukemia, lung , bladder, brain, stomach, pancreas, urinary, colon and bone.( 4 )- There exist significant different between all cancers and the more significant are leukemia and lunge.(5)-There are significant different between female cancers and the more significant are breast, uterus and leukemia. (6)- There exist significant difference between male cancers and the more significant are leukemia and lunge.

Effect of Coffee Ages and Shade Types on Soil Moisture and Soil N, P, K Availability in UB Forest

Water and nutrients is important for plant which has its characteristics. This study has been carried out in the UB (Brawijaya University) forest about soil moisture content during rainy and dry seasons, Total-N, -P, -K from litter on different types of shade and age of coffee plants, and their effect on soil Available-N, -P, and -K. There are P1 (control), P2 (4-year-coffee + pine), P3 (7-year-coffee + pine), P4 (4-year-coffee + mahogany), and P5 (7-year-coffee + mahogany). These treatments affected to total soil moisture storage, Total-N, -P, -K of litter, and Available-N, -P, -K in the soil. The soil moisture storage change between rainy and dry seasons was 36.97 mm. The highest Total-N, -P, and -K of litter was 11.00 kg ha-1 y-1 at P3, 1.06 kg ha-1 y-1 at P2, and 4.35 kg ha-1 y-1 at P3, which was inconsistently associated with high soil nutrients. The highest Total-N was 0.40 (0-20 cm), 0.27 (20-40 cm), 0.30% (40-60 cm) at P4, Available-K was 1.69 (0-20 cm), 1.64 (20-40 cm), 1.87 cmol kg-1 (40-60 cm) at P5, and Available-P was 15.21 (0-20 cm), 14.06 (20-40 cm), 12.64 mg kg-1 (40-60 cm) at P1.

The molecular mechanism of glycation. Harmful effects of advanced glycation end products (AGEs) on the skin

Non-enzymatic glycosylation, commonly known as glycation, is a physiological process that may be intensified by endogenous and exogenous factors. The accumulation of advanced glycation end products (AGEs) effects negatively the aging process of the skin, modifying the biochemical, structural, and morphological properties of the skin. The study aimed to describe the mechanisms and effects of AGEs on the cells of the epidermis, dermis, vascular endothelium, as well as extracellular matrix proteins and intercellular lipids. Scientific research is being conducted to find substances that effectively inhibit the glycation process and the accompanying glycooxidation.

Effect of Different Black Pepper Crop Ages on the Availability of Nitrogen, Phosphorus, and Potassium

Black pepper cultivation at different land backgrounds and fertility can affect crop productivity. This study aims to determine soil-plant nutrients for three pepper farms based on crop ages and the correlation of selected physical and chemical properties. Samples were collected in Kapit, Bintulu, and Sri Aman and analyzed for physical and chemical properties. Soil nutrient in Bintulu (6 months old) has revealed higher soil pH (4.95) with higher Fe (0.21 g/kg) and N (1.33 g/kg) contents that is caused by burnt earth residue from the burning of secondary forest. Sri Aman (12 months old) showed greater TOC (3.34%), P (0.0032 g/kg), and K (0.10 g/kg) benefitted from a long year of cultivation and proper pre-establishment earlier. Nutrient removal is high in Bintulu for Fe, Mn, N, and K, while Sri Aman for P is due to early harvesting before its mature phase, whereby the increase in nutrient accumulation in leaves to support berries. Correlation has revealed the role of soil pH in governing the availability of TOC, P, and K. The increasing soil pH increases the availability of P and K. Hence, the availability of N, P, and K is affected by numerous reasons, such as pH and crop management.

Effects of e-cigarette minimum legal sales ages on youth tobacco use in the United States

Effects of e-cigarette minimum legal sales ages on youth tobacco use in the United States