Various probing beams have been developed and used for plasma diagnostics in thermonuclear fusion research for magnetic confinement. The required beam current, beam size, energy, beam characteristics such as divergence, energy spread, and emittance, etc., as well as ion species are different depending on the purpose of diagnostics, target plasma size and parameters. Hydrogen beams are used for charge-exchange recombination spectroscopy (CHERS) and motional stark effect (MSE) spectroscopy. Suitable beam energy for CHERS ranges in the area of 50 keV/amu, and a small beam divergence is required for MSE measurements. Heating beams are often used as diagnostic beams by sharing. Neutral lithium probing beams have been used as effective tools for measurement of density and fluctuations. A heavy ion beam probe (HIBP), made from alkali metal ions, is injected directly without neutralization to measure plasma potential and density, as well as their fluctuations. Recent progress in magnetic fusion experiments has resulted in advancement of the development of negative ion sources, such as He/sup -/ and Li/sup -/ sources for alpha-particle measurement, because of their advantages with regard to efficient neutralization in the high-energy region, and an Au/sup -/ source for HIBP with the application of tandem acceleration.