Objectives It is expected that Alpha and SMR activity would be enhanced (Vernon et al., 2003), whereas Theta activity would be diminished (Hammond, 2005) in adolescents in line with previous studies on this population. Methods Ten adolescents from 11 to 18 years old came to the Northern Greece Neurofeedback Centre because they had problems about their self-reported attention, as well as having anxiety symptoms, including daydreaming, loss of concentration during reading, and thoughts of worry. Neurofeedback training consisted of 10 sessions, 20 minutes each. Participants’ EEG records were evaluated pre and post the Neurofeedback training in eyes-opened resting-state by Nexus-10. In continuation, they were trained on Alpha, Theta and Beta bands through specific protocols. The active electrode was placed to the Cz point with frequency rate two times/week. Additionally, individuals completed two Visual Analogue Scales (VAS), scored from 0–100, before each Neurofeedback session regarding to whether they felt anxiety and felt absent- minded during the previous days. Results Non parametric Wilcoxon signed-rank test was used in order to identify possible differences between the post amplitude of the EEG brain rhythms, especially Alpha, Theta and SMR. After attending the Neurofeedback sessions, participants’ Alpha and SMR bands were enhanced. Specifically, significant differences were found in Alpha mean amplitude (z = − 2.366; P = .018), as well as SMR activity (z = −2.666; P = .008). Furthermore, no significant differences were found for Theta. VAS for attention was significantly increased (z = −2.197; P = .030), whereas VAS for anxiety was statistically decreased (z = −2.265; P = .023). Conclusion Neurofeedback can be regarded as a promising therapeutic technique at reducing anxiety symptoms and attention deficits in adolescents. However, future research should be conducted in order to investigate the effectiveness of Neurofeedback in alleviating anxiety and attention deficits in adolescents by recruiting a larger number of individuals and over a longer period of time.