Fine bubbles (FBs) are defined by the ISO/TC 281 as gas bubbles with a diameter of less than 100 μm, and they have interesting properties such as high surface-to-volume ratio, low buoyancy, long residence time, electric charge, and self-pressurization effect. Typically, FBs are characterized in terms of size distribution, concentration, and zeta potential through specialized microscopic and nanoscopic measuring devices. This work proposes a multi-objective optimization problem to find the optimal conditions to generate FBs from experimental macroscopic measurements in terms of dissolved oxygen (DO). Then, detailed microscopic measurements in terms of size distribution and zeta potential are conducted. Additionally, two venturi-type Fine Bubble Generators (FBGs) were 3D-printed in-house to evaluate the relationship between the internal structure and the generation of FBs. The best FBGs have an obstacle in the diverging section that promotes FB generation under the evaluated experimental conditions. Under the best operating conditions, FBs were stable over 7 days with a size distribution between 60 and 90 nm and with an average of −21 mV.
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