Analysis Effect Size Research Articles

The impact of physical and psychological pain management training on pain intensity, anxiety and disability in patients undergoing lumbar surgeries

BACKGROUND/CONTEXTLumbar surgery is one of the interventions performed for patients with degenerative conditions. PURPOSEThis study aimed to investigate the effect of pain management education on pain intensity, anxiety, and disability after the lumbar surgery. STUDY DESIGN/SETTINGThis randomized controlled trial was performed on 70 30-65-year-old patients with lumbar canal stenosis and lumbar disc herniation from 2018 to 2019. PATIENT SAMPLESeventy participants were randomly divided into a control and an intervention group by a randomized block design. Participants in the intervention group received in-person pain management training twice a week for seven 60-90-minute sessions. OUTCOME MEASURESAll participants in the two groups completed the study instruments (numeric rating scale [NRS], Oswestery disability index [ODI], and pain anxiety symptoms scale [PASS]) before, immediately after, and 3 months after the study. METHODSParticipants in the intervention group received in-person pain management training twice a week for seven 60-90-minute sessions. To analyze the treatment effects, repeated-measures multivariate analysis of variance (MANOVA) and effect sizes were used where appropriate and calculated by Partial ɳ2. Clinical outcome (MDC) for pain intensity and PASS was also reported. For participants lost to follow-up, we also used an “intention-to-treat” (ITT) approach. RESULTSThe results of MANOVA indicated that there were significant differences between the two groups on ratings of pain intensity, anxiety, and disability. According to the MDC, the mean differences of pain intensity for the intervention group was also clinically improved. Meanwhile, the mean differences in pain anxiety between three different times in the two groups were not above the MDC (20.14), suggesting that the clinical improvements were not significant. The results were confirmed for all outcome measures; a statistically significant difference was found between the groups in ITT analyses (p<.001). CONCLUSIONSPhysical and psychological pain management education was shown to be effective in decreasing pain intensity, anxiety, and disability. This strategy may be beneficial for such patients. Variables such as smoking behavior, past history of psychological disorders, and previous surgeries should be considered in future studies.

Amplicon-based next-generation sequencing for comparative analysis of root canal microbiome of teeth with primary and persistent/secondary endodontic infections.

To compare the root canal microbiome profiles of primary and persistent/secondary infections using high-throughput sequencing with the help of a reliable bioinformatics algorithm. Root canal samples of 10 teeth in the primary endodontic infection (PEI) group and 10 teeth in the persistent/secondary endodontic infection (SEI) group were included resulting in a total of 20 samples. After DNA extraction from the samples, sequencing was performed on the Illumina MiSeq platform. Pair-end Illumina reads were imported to QIIME 2; amplicon sequence variants (ASVs) generated by DADA2 were mapped to GreenGenes database. Weighted UniFrac distances were calculated and principal coordinates analysis (PCoA) was used to compare beta diversity patterns. The multiple response permutation procedure (MRPP), the analysis of similarities (ANOSIM), and permutational multivariate analysis of variance (adonis) were conducted for testing group differences. Linear discriminant analysis effect size (LEfSe) analysis was utilized to identify differentially abundant taxa between the groups. The linear discriminant analysis (LDA) score threshold was set to 4.0. Within the Gram-negative facultative anaerobic Gammaproteobacteria class outgroup, two orders (Pasteurellales, Vibrionales) and two families (Pasteurellaceae, Vibrionaceae) were significantly more abundant in the PEI group, whereas Gram-positive bacteria, Actinomycetales order, and Gram-positive anaerobic taxa, one genus (Olsenella) and one species (Olsenella uli), were identified as significantly more abundant in the SEI group. A few taxa were differentially abundant within either the PEI or SEI group. Reliable bioinformatic tools are needed to define microbial profiles of endodontic infections. Based on a limited number of samples, no distinct variation was determined between the bacterial diversity of initial and recurrent endodontic infections.

Three-dimensional printing of patient-specific computed tomography lung phantoms: a reader study.

In modern clinical decision-support algorithms, heterogeneity in image characteristics due to variations in imaging systems and protocols hinders the development of reproducible quantitative measures including for feature extraction pipelines. With the help of a reader study, we investigate the ability to provide consistent ground-truth targets by using patient-specific 3D-printed lung phantoms. PixelPrint was developed for 3D-printing lifelike computed tomography (CT) lung phantoms by directly translating clinical images into printer instructions that control density on a voxel-by-voxel basis. Data sets of three COVID-19 patients served as input for 3D-printing lung phantoms. Five radiologists rated patient and phantom images for imaging characteristics and diagnostic confidence in a blinded reader study. Effect sizes of evaluating phantom as opposed to patient images were assessed using linear mixed models. Finally, PixelPrint's production reproducibility was evaluated. Images of patients and phantoms had little variation in the estimated mean (0.03-0.29, using a 1-5 scale). When comparing phantom images to patient images, effect size analysis revealed that the difference was within one-third of the inter- and intrareader variabilities. High correspondence between the four phantoms created using the same patient images was demonstrated by PixelPrint's production repeatability tests, with greater similarity scores between high-dose acquisitions of the phantoms than between clinical-dose acquisitions of a single phantom. We demonstrated PixelPrint's ability to produce lifelike CT lung phantoms reliably. These phantoms have the potential to provide ground-truth targets for validating the generalizability of inference-based decision-support algorithms between different health centers and imaging protocols and for optimizing examination protocols with realistic patient-based phantoms. Classification: CT lung phantoms, reader study.

P923 The pre-treatment gut microbiome at adult IBD inception: Initial signals from the Birmingham inception cohort

Abstract Background Gut microbiome disruption and ‘dysbiosis’ is an established characteristic of IBD. The lack of treatment naïve and prospective longitudinal microbiome data makes it challenging to separate the influence of potential confounders. We present our preliminary microbiota analysis from a treatment naïve cohort of IBD patients seen in our rapid-access integrated clinical and research IBD inception clinic. Methods Stool collected in DNA Genotek OM-200 kits is brought to the first face to face pre-diagnosis outpatient review, alongside faecal calprotectin (FCAL). Metadata is collected prospectively with repeat samples collected longitudinally. Healthy controls were recruited separately. Stool microbial DNA was extracted with subsequent 16S rRNA PCR and sequencing. Diversity analysis and taxonomic classifications were performed in line with the QIIME2 workflow. Taxa with linear discriminant analysis (LDA) &amp;gt;2 at a P&amp;lt;0.05 were considered significant on LDA effect size (LEfSe) analysis. Correlation was evaluated with Kendall’s Tau coefficient. Results Across sites, our clinics have seen 609 patients, with 256 enrolled in active research. We have banked 200 pre-treatment OM-200 stool samples from this cohort, with a further 45 samples from follow up visits. Of the first 49 samples sequenced, 31 were diagnosed with IBD (UC=18; CD=13). An independent cohort of 18 healthy controls were used for comparison. Median FCAL in the IBD cohort was 915ug/g (IQR 1499ug/g). Microbial composition in the IBD patients as assessed by Bray-Curtis distance matrix was significantly different compared to healthy controls (P&amp;lt;0.001). Alpha diversity (Shannon diversity index) was significantly lower in the IBD cohort when compared to healthy controls (P=0.01, Figure 1). These differences in alpha and beta diversity were also observed with IBD subtypes (P&amp;lt;0.01). Patients with IBD had significantly lower relative abundance of multiple taxa belonging to the families Clostridiaceae, Lachnospiraceae, and Coriobacteriaceae and genus Ruminococcus, Butyricicoccus, Bifidobacterium, Blautia and Dorea, as displayed in Figure 2. The genuses Fusobacterium, Peptostreptococcus and Subdoligranulum positively correlated with FCAL levels whilst the genus Lachnobacterium negatively correlated (both P&amp;lt;0.05). Conclusion We present our preliminary index analysis of microbiome data in a unique treatment naïve IBD cohort. Our ongoing prospective longitudinal collection of clinical, microbial, immunological and metabolomic datasets will facilitate understanding of potential baseline prognostic indicators, predictive biomarkers for treatment response and relationship with disease activity. A further large batch of samples are submitted for sequencing and additional insights will follow.

Average Daily Gain in Lambs Weaned at 60 Days of Age Is Correlated with Rumen and Rectum Microbiota.

Colonization of gastrointestinal microbiota in mammals during early life is vital to host health. The objective of this study was to investigate whether lambs with high and low ADG have a different rumen and rectum microbial community. Thus, we investigated potential relationships between rumen and rectum microbiota and average daily gain (ADG) in weaned lambs. Sixteen lambs with similar body weights (7.63 ± 1.18 kg) were selected at 30 days of age. At 60 days of age, lambs were weaned, and ADG was calculated from 60 to 90 days. Then, two groups were generated: higher ADG (HG, 134.17 ± 13.48 g/day) and lower ADG (LG, 47.50 ± 19.51 g/day). Microbiota was evaluated at 30, 60, and 90 days of age. The final live weight and ADG at 90 days of age was higher (p < 0.05) in the HG group compared to the LG group. The maturity of bacterial and fungal communities was increased (p < 0.05) in the HG group for the 30 days vs. 90 days comparison and 60 days vs. 90 days comparison. Linear discriminant analysis effect size (LEfSe) analysis revealed a total of 18 bacterial biomarkers that are ADG-specific in the rumen and 35 bacterial biomarkers in the rectum. Meanwhile, 15 fungal biomarkers were found in the rumen and 8 biomarkers were found in the rectum. Our findings indicated that ADG is related to the rumen and rectum microbiota in lambs.

기업가정신이 기업성과에 미치는 영향에 관한 메타연구

This study conducted a meta-analysis on the effects of initiative, risk tolerance, and innovation, which are the components of entrepreneurship, on business performance. Among previous studies on the effect of entrepreneurship on corporate performance, a total of 51 theses from doctoral dissertations published in South Korea between 2000 and 2022 were selected as study subjects. Among those theses, 39 were finally selected after excluding 12 theses considered insufficient to be used as research data since they lacked stat ist ics, such as correlation coefficient. An average effect size analysis, publication error analysis, and regression analysis were conducted regarding the variables for the 39 finally selected theses. The analysis results of this study are as follows; First, as a result of analyzing the average effect size using a forest plot, all presented static results. Second, the publication error analysis resulted in no publication error since the diagram (funnel plot) was symmetrical vertically in the center. Third, the results of regression analysis on the effect of entrepreneurship on financial performance were all statistica lly significant. This study implies that academic significance has been brought since many researchers identified the relationship between entrepreneurship and entrepreneurial performance through empirical studies. Entrepreneurship, derived from the research results, is a capability required from members and entrepreneurs who prepare to open a business as well as from CEOs of companies. This study intends to enable entrepreneurship to suggest a direction for companies and present the grounds for establishing government policies to enhance entrepreneurship in the future.

Association of gut microbiota and glucose metabolism in children with disparate degrees of adiposity.

To investigate the characteristics of gut microbiota in children with disparate degrees of adiposity, and analyze the association between gut microbiota, glucose metabolism indicators, and inflammatory factors. Clinical data were examined in 89 Chinese children. Children with a body fat percentage ≥ 30% were diagnosed as obese, and ≥ 35% in males and ≥ 40% in females were further defined as severe obesity. The composition of gut microbiota was determined by 16S rDNA-based metagenomics. The study population (9.75 ± 1.92-year-old) was characterized as normal weight (n=29), mild obesity (n=27) and severe obesity (n=33) groups. Linear discriminant analysis Effect Size (LEfSe) analysis found that compared to the severe obesity group, subjects with mild obesity had more prevalent members of the phylum Fusobacteria, the genus Alistipes, and fewer members of genus Granulicatella and Clostridium (p < 0.05). For subjects with mild obesity, Spearman's correlation analysis revealed that fasting plasma glucose positively correlated with species A.indistinctus, A.putredinis, and negatively correlated with species Ruminococcus gnavus; LBP negatively correlated with species Clostridium hathewayi, and Blautia producta. For subjects with severe obesity, oral glucose tolerance test 2h plasma glucose (OGTT2HPG) negatively correlated with the phylum Synergistetes, genus Pyramidobacter, species Veillonella parvula, P.piscolens, and positively correlated with species B.producta, INS and HOMA-IR negatively correlated with the genus Haemophilus, species H. parainfluenzae, lipopolysaccharide-binding protein (LBP) negatively correlated with the phylum Actinobacteria, genus Bifidobacterium, Lactobacillus, and species B.longum (all p < 0.05). Phylogenetic investigation of communities by reconstruction of unobserved states 2 (PICRUSt2) analysis discerned that the glucose metabolism pathway, gluconeogenesis I was curtailed in the severe obesity group. The gut microbiota could favourably compensate for glucose metabolism in children with obesity. Genus Haemophilus and Bifidobacterium longum may influence glucose tolerance and insulin resistance in children with severe obesity.

Comparison of Bacterial Diversity in the Rhizosphere of Chromolaena odorata (L.) R.M. King and H.Rob. in Different Habitats

The invasion of Chromolaena odorata (L.) R.M. King and H.Rob. affects microbial community structure, but seldom do studies focus on bacterial diversity in the rhizosphere of C. odorata from different habitats. To reveal the impacts of C. odorata on bacterial communities in different habitats, diversity and functional groups of bacteria were compared in the rhizosphere soil of C. odorata collected from three habitats: abandoned land, a mature eucalyptus forest (eight-years), and a young eucalyptus forest (one-year). In this study, 3701 OTUs (operational taxonomic units) were obtained by next-generation sequencing. Simpson diversity indexes of bacteria in eucalyptus forests were significantly higher than that in abandoned land. Based on PCoA analysis, significant differences in the composition of bacterial communities were observed among the three habitats. Significant differences in bacterial communities among the three habitats were also demonstrated by biomarkers based on linear discriminant analysis effect size (LEfSe) analysis. Functional prediction analysis showed that the abundance of nitrogen-fixing functional genes in eucalyptus forests was greater than that in abandoned land. Canonical correspondence analysis (CCA) found that the biomass of C. odorata, soil pH, and total phosphorus were the main factors that impacted the microbial community structure. The results highlight the microbial community varied with habitats invaded by C. odorata, providing new evidence for understanding the invasion of C. odorata in different habitats.

The Adaptive Evolution in the Fall Armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae) Revealed by the Diversity of Larval Gut Bacteria.

Insect gut microbes have important roles in host feeding, digestion, immunity, development, and coevolution with pests. The fall armyworm, Spodoptera frugiperda (Smith, 1797), is a major migratory agricultural pest worldwide. The effects of host plant on the pest's gut bacteria remain to be investigated to better understand their coevolution. In this study, differences in the gut bacterial communities were examined for the fifth and sixth instar larvae of S. frugiperda fed on leaves of different host plants (corn, sorghum, highland barley, and citrus). The 16S rDNA full-length amplification and sequencing method was used to determine the abundance and diversity of gut bacteria in larval intestines. The highest richness and diversity of gut bacteria were in corn-fed fifth instar larvae, whereas in sixth instar larvae, the richness and diversity were higher when larvae were fed by other crops. Firmicutes and Proteobacteria were dominant phyla in gut bacterial communities of fifth and sixth instar larvae. According to the LDA Effect Size (LEfSe) analysis, the host plants had important effects on the structure of gut bacterial communities in S. frugiperda. In the PICRUSt2 analysis, most predicted functional categories were associated with metabolism. Thus, the host plant species attacked by S. frugiperda larvae can affect their gut bacterial communities, and such changes are likely important in the adaptive evolution of S. frugiperda to host plants.

Diversity of the fecal microbiota in Chinese ponies.

The gut microbiomes of equine are plentiful and intricate, which plays an important part in the growth. However, there is a relative lack of information on the microbial diversity in the pony's gut. In this article, 118 fecal samples from DeBa pony, NiQi pony and GuZh horse were studied by 16S rRNA amplicon sequencing. Diversity analysis was used to determine the difference of gut microbiota composition among different breeds. Alpha diversity analysis showed that the gut microbiota of NiQi ponies were abundant and various. Beta diversity analysis showed that the microorganisms constitution of DeBa ponies was more similar to that of NiQi ponies. LDA Effect Size (LEfSe) analysis result that the microorganism biomarkers for NiQi pony at the genus level were Phascolarctobacterium, Paludibacter, and Fibrobacter; the bacterial biomarker for DeBa pony was Streptococcus and Prevotella; and the bacterial biomarkers for GuZh horses was Treponema, Treponema Mogibacterium, Adlercreutzia, and Blautia. The correlation analysis between genera with >1% abundance and horse height found that Streptococcus (P < 0.01), Treponema (P < 0.01), Coprococcus (P < 0.01), Prevotella (P < 0.01), Phascolarctobacterium (P < 0.01), and Mogibacterium (P < 0.01) were significantly associated with horses' height. The functional prediction results indicated that DeBa pony have a microbiota functional more similar to NiQi pony. For the first time, our results announce the species composition and structure of the gut microbiota in Chinese ponies. At the same time, our results can provide theoretical reference for further understanding the healthy breeding, feeding management and disease prevention of horses.

Comparison of gut microbiota between immigrant and native populations of the Silver-eared Mesia (Leiothrix argentauris) living in mining area.

The complex gut bacterial communities have a major impact on organismal health. However, knowledge of the effects of habitat change on the gut microbiota of wild birds is limited. In this study, we characterized the gut microbiota of two different subspecies of the Silver-eared Mesia (Leiothrix argentauris), the native subspecies (L. a. rubrogularis) and immigrant subspecies (L. a. vernayi), using 16S rRNA gene high-throughput sequencing. These two subspecies live in a trace metal-contaminated area, and L. a. vernayi was trafficked. They are an excellent system for studying how the gut microbiome of wild animal changes when they move to new habitats. We hypothesized that the immigrant subspecies would develop the same adaptations as the native subspecies in response to habitat changes. The results showed that there were no significant differences in the composition, diversity, or functional metabolism of gut microbiota between native and immigrant subspecies under the combined action of similar influencing factors (the p values of all analyses of variance >0.05). In addition, the composition and functional metabolism of gut microbiota in two subspecies showed adaptation against trace metal damage. Linear discriminant analysis effect size (LEfSe) analysis revealed that Massilia in the intestinal microbiota of immigrant subspecies was significantly higher than that of native subspecies, suggesting that immigrant subspecies suffered habitat change. Finally, we found that these two subspecies living in the mining area had an extremely high proportion of pathogenic bacteria in their gut microbiota (about 90%), much higher than in other species (about 50%) living in wild environment. Our results revealed the adaptation of intestinal microbiota of immigrant Silver-eared Mesias under heavy metals stress, which would provide guidance for biodiversity conservation and pollution management in mining area.

Effects of Occupational Load on the Acceleration, Change of Direction Speed, and Anaerobic Power of Police Officers.

Kukić, F, Janković, R, Dawes, JJ, Orr, R, and Koropanovski, N. Effects of occupational load on the acceleration, change of direction speed, and anaerobic power of police officers. J Strength Cond Res 37(6): 1237-1243, 2023-Police officers engage in movements such as foot pursuits, sudden directional changes, or prolonged sprinting while wearing occupational loads (including body armor, baton, radio, handcuffs, and sidearm). This study investigated the effects of incrementally heavier loads carried by police students on their physical performance. Police students ( n = 63; male n = 39 male and 24 female students) performed an acceleration over a 10-m distance (Acc 10m ), Illinois agility test (IAT), and 300-yard shuttle run test (ShR 300y ) without load, with a 5-kg load (handgun, spare empty magazine, baton, and handcuffs), and with a 10-kg load (loaded vest). The effects of different load conditions were investigated for both sexes using a repeated-measure analysis of variance and Cohen's effect size. Female students carried relatively heavier loads and were slower than male students in Acc 10m , IAT, and ShR 300y . Load significantly ( p < 0.001) impaired the performance in Acc 10m , IAT, and ShR 300y in both sexes, but the effects were greater ( p < 0.001) among female police students. A load as little as 5 kg was sufficient to reduce Acc 10m , IAT, and ShR 300y among police students. Increasing the load to 10 kg (i.e., wearing the body armor) did not significantly affect Acc 10m and IAT among male students, but it did affect Acc 10m and IAT among female students. Sprint endurance (i.e., ShR 300y ) was strongly affected by both loads regardless of sex. Strength and conditioning programs should focus on strength, power, and anaerobic endurance to improve performance while carrying occupational loads. Occasional training with loads similar to those at work may further ensure that officers are fit for purpose.

Changes in Phyllosphere Microbial Communities of Pinus tabuliformis after Infestation by Bursaphelenchus xylophilus

Phyllosphere microbial communities have an important role in plant growth and resistance to pathogen infection and are partially influenced by leaf characteristics. Pinewood nematode, Bursaphelenchus xylophilus, is one of the greatest threats to pine trees and is spreading all over the world. However, studies on the resistance of plant–microbe interactions to pathogens during the nematode’s pathogenesis and the relationships of leaf chemical characteristics caused by pinewood nematode and phyllosphere microbial communities are limited. In this study, different stages of Pinus tabuliformis that were healthy or infected with B. xylophilus-associated leaf characteristics and phyllosphere bacterial and fungal communities were compared. These results demonstrated that soluble sugar and starch contents decreased based on the extent of infection. Phyllosphere microbial community changes potentially caused by B. xylophilus infection of P. tabuliformis and the fungal community compositions of healthy P. tabuliformis trees (Ya) were clearly different from diseased P. tabuliformis trees at an early stage of B. xylophilus infection (Yb) and P. tabuliformis trees in the last stage of B. xylophilus infection (Yc), particularly along the first coordinate axis. According to a linear discriminant effect size (LEfSe) analysis, the biomarker species in the phyllosphere of Yb were Acidobacteria, Deinococcus-Thermus, and Patescibacteria, while those in the phyllosphere of Ya were Proteobacteria, Aureobasidium, Dictyosporium, Alternariaster, Knufia, Microstroma, and Naganishia. Particularly at the end of PWD (pine wilt disease) infection, the majority of microbial taxa tended to co-exclude rather than co-occur with PWD infection. The result of a canonical correlation analysis (CCA) showed that the chemical properties of leaves, such as carbon and nitrogen, have significant impacts on phyllosphere microbial communities. These results expanded the possible connections between the phyllosphere communities and plant health.

Grifola frondosa may play an anti-obesity role by affecting intestinal microbiota to increase the production of short-chain fatty acids.

Grifola frondosa (G. frondosa) is a fungus with good economic exploitation prospects of food and medicine homologation. This study aims to investigate the effects of G. frondosa powder suspension (GFPS) on the intestinal contents microbiota and the indexes related to oxidative stress and energy metabolism in mice, to provide new ideas for developing G. frondosa weight loss products. Twenty Kunming mice were randomly divided into control (CC), low-dose GFPS (CL), medium-dose GFPS (CM), and high-dose GFPS (CH) groups. The mice in CL, CM, and CH groups were intragastrically administered with 1.425 g/(kg·d), 2.85 g/(kg·d), and 5.735 g/(kg·d) GFPS, respectively. The mice in CC group were given the same dose of sterile water. After 8 weeks, liver and muscle related oxidative stress and energy metabolism indicators were detected, and the intestinal content microbiota of the mice was detected by 16S rRNA high-throughput sequencing. After eight weeks of GFPS intervention, all mice lost weight. Compared with the CC group, lactate dehydrogenase (LDH) and malondialdehyde (MDA) contents in CL, CM, and CH groups were increased, while Succinate dehydrogenase (SDH) and Superoxide Dismutase (SOD) contents in the liver were decreased. The change trends of LDH and SDH in muscle were consistent with those in the liver. Among the above indexes, the change in CH is the most significant. The Chao1, ACE, Shannon, and Simpson index in CL, CM, and CH groups were increased. In the taxonomic composition, after the intervention with GFPS, the short-chain fatty acid (SCFA)-producing bacteria such as unclassified Muribaculaceae, Alloprevotella, and unclassified Lachnospiraceae increased. In linear discriminant analysis effect size (LEfSe) analysis, the characteristic bacteria in CC, CL, CM, and CH groups showed significant differences. In addition, some characteristic bacteria significantly correlated with related energy metabolism indicators. The preventive effect of G. frondosa on obesity is related to changing the structure of intestinal content microbiota and promoting the growth of SCFAs. While excessive intake of G. frondosa may not be conducive to the antioxidant capacity and energy metabolism.

Vaginal Microbiota Changes in the Vulvar Lichen Simplex Chronicus

Background: The vulvar lichen simplex chronicus (VLSC) is a common condition in gynecologic clinics. Though VLSC is not life-threatening, it usually causes pruritus, soreness and dispareunia, which cause general discomfort. The exact etiology of VLSC is unclear. This study was performed to explore the vaginal microbiota of VLSC and to identify the possible microbial factors in attacks. Methods: Ninety women were recruited. 45 patients with VLSC and 45 women without vulvar symptom were identified as lichen simplex chronicus (LSC) and H groups respectively. The vaginal microbiota of the two arms were compared by the V3-V4 region of 16S rRNA sequencing. Results: The LSC group had less alpha diversity than H group (p &lt; 0.05) and the beta diversity of LSC group was also distinct from the H group. Linear discriminant analysis effect size (LEfSE) analysis indicated that genus Sneathia and family Leptotrichiaceae were discriminant taxa in LSC group. Phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) analysis found that microbial genes related to the signal transduction, metabolism of terpenoids and polyketides, transporters, nervous system, energy metabolism and others were different in the LSC and H groups. Conclusions: VLSC was associated with dysbioss of vaginal microbiota profiles compared with healthy control cases.

Alteration of the gut microbiota and metabolite phenylacetylglutamine in patients with severe chronic heart failure.

Chronic Heart Failure (CHF) is the end result of nearly all cardiovascular disease and is the leading cause of deaths worldwide. Studies have demonstrated that intestinal flora has a close relationship with the development of Cardiovascular Disease (CVD) and plays a vital role in the disease evolution process. Phenylacetylglutamine (PAGln) a metabolite of the intestinal flora, is one of the common chronic kidney disease toxins. Its concentrations in plasma were higher in patients with major adverse cardiovascular events (MACE) however, its variation in patients with various degrees of CHF has rarely been reported. Therefore, we collected stool and plasma samples from 22 healthy controls, 29 patients with NYHA Class III and 29 patients with NYHA Class IV CHF (NYHA stands for New York Heart Association) from the Department of Cardiology of Shanghai Fengxian District Central Hospital. Next, we analyzed these samples by performing bacterial 16S ribosomal RNA gene sequencing and liquid chromatography tandem mass spectrometry. The result shows: The Chao 1 index was significantly lower in both NYHA class III and NYHA class IV than it was in the control group. The beta diversity was substantially dissimilar across the three groups. The linear discriminant analysis effect size analysis (LEfSe) showed that the bacterial species with the largest differences were Lachnospiraceae in control group, Enterobacteriaceae in NYHA class III, and Escherichia in NYHA class IV. The concentration of PAGln was significantly different between CHF and control groups and increased with the severity of heart failure. Finally, the correlation analysis represented that Parabacteroides and Bacteroides were negatively correlated to brain natriuretic peptide (BNP) and PAGln; Romboutsia and Blautia adversely associated with PAGln; Klebsiella was positively interrelated with BNP; Escherichia-Shigella was positively correlated with PAGln and BNP; Alistipes was contrasted with BNP; and Parabacteroides was negatively correlated with the left ventricular end-diastolic diameter (LVEDD). This study presented that the intestinal flora and its metabolite PAGln were altered with different grades of CHF and illustrated the effects of the gut flora and its metabolite on CHF.

The host genetics affects gut microbiome diversity in Chinese depressed patients.

The gut microbiome and host genetics are both associated with major depressive disorder (MDD); however, the molecular mechanisms among the associations are poorly understood, especially in the Asian, Chinese group. Our study applied linear discriminant analysis (LDA) effect size (LEfSe) and genome-wide association analysis in the cohort with both gut sequencing data and genomics data. We reported the different gut microbiota characteristics between MDD and control groups in the Chinese group and further constructed the association between host genetics and the gut microbiome. Actinobacteria and Pseudomonades were found more in the MDD group. We found significant differences in the ACE and Chao indexes of alpha diversity while no discrepancy in beta diversity. We found three associations between host genetics with microbiome features: beta diversity and rs6108 (p = 8.65 × 10-9), Actinobacteria and rs77379751 (p = 8.56 × 10-9), and PWY-5913 and rs1775633082 (p = 4.54 × 10-8). A species of the Romboutsia genus was co-associated with the species of Ruminococcus gnavus in an internetwork through four genes: METTL8, ITGB2, OTULIN, and PROSER3, with a strict threshold (p < 5 × 10-4). Furthermore, our findings suggested that the gut microbiome diversity might affect microRNA expression in the brain and influenced SERPINA5 and other spatially close genes afterward. These findings suggest new linkages between depression and gut microbiome in Asian, Chinese people, which might be mediated by genes and microRNA regulation in space distance.

Anti-PD-L1 Antibody and/or 17β-Estradiol Treatment Induces Changes in the Gut Microbiome in MC38 Colon Tumor Model.

17β-Estradiol (E2) supplementation suppresses MC38 tumor growth by downregulating the expression of programmed death-ligand 1 (PD-L1). This study aims to figure out the gut microbiota that respond to anti-PD-L1 and/or estrogen treatment in MC38 colon cancer model. A syngeneic colon tumor model was developed by injection of MC38 cells into C57BL/6 background male and female mice. Three days before MC38 cells injection, E2 was supplemented to male mice daily for 1 week. Male and female mice with MC38 tumors (50-100 mm3) were injected with anti-PD-L1 antibody. Fresh feces were collected 26 days after injection of MC38 cells and 16S rRNA metagenomics sequencing of DNA extracted from feces was used to assess gut microbial composition. At the taxonomic family level, Muribaculaceae was enriched only in the MC38 male control group. In male mice, linear discriminant analysis effect size analysis at the species level revealed that the four microorganisms were commonly regulated in single and combination treatment with anti-PD-L1 and/or E2; a decrease in PAC001068_g_uc and PAC001070_s (family Muribaculaceae) and increase in PAC001716_s and PAC001785_s (family Ruminococcaceae). Interestingly, in the anti-PD-L1 plus E2 group, a decrease in opportunistic pathogens (Enterobacteriaceae group) and an increase in commensal bacteria (Lactobacillus murinus group and Parabacteroides goldsteinii) were observed. Furthermore, the abundance of Parabacteroides goldsteinii was increased in both males and females in the anti-PD-L1 group. Our results suggest that gut microbial changes induced by the pretreatment of estrogen before anti-PD-L1 might contribute to treatment of MC38 colon cancer.

Response of soil bacterial community to alpine wetland degradation in arid Central Asia.

A large number of studies have reported the importance of bacterial communities in ecosystems and their responses to soil degradation, but the response mechanism in arid alpine wetlands is still unclear. Here, the non-degraded (ND), slightly degraded (SD), and heavily degraded (HD) regions of Bayinbuluk alpine wetland were used to analyzed the diversity, structure and function of bacterial communities in three degraded wetlands using 16S rRNA. The results showed that with the increase of degradation degree, the content of soil moisture (SM) and available nitrogen (AN) decreased significantly, plant species richness and total vegetation coverage decreased significantly, Cyperaceae (Cy) coverage decreased significantly, and Gramineae (Gr) coverage increased significantly. Degradation did not significantly affect the diversity of the bacterial community, but changed the relative abundance of the community structure. Degradation significantly increased the relative abundance of Actinobacteria (ND: 3.95%; SD: 7.27%; HD: 23.97%) and Gemmatimonadetes (ND: 0.39%; SD: 2.17%; HD: 10.78%), while significantly reducing the relative abundance of Chloroflexi (ND: 13.92%; SD: 8.68%; HD: 3.55%) and Nitrospirae (ND: 6.18%; SD: 0.45%; HD: 2.32%). Degradation significantly reduced some of the potential functions in the bacterial community associated with the carbon (C), nitrogen (N) and sulfur (S) cycles, such as hydrocarbon degradation (ND: 25.00%; SD: 1.74%; HD: 6.59%), such as aerobic ammonia oxidation (ND: 5.96%; SD: 22.82%; HD: 4.55%), and dark sulfide oxidation (ND: 32.68%; SD: 0.37%; HD: 0.28%). Distance-based redundancy analysis (db-RDA) results showed that the bacteria community was significantly related to the TC (total carbon) and Gr (P < 0.05). The results of linear discriminant analysis effect size (LEfSe) analysis indicate significant enrichments of Alphaproteobacteria and Sphingomonas in the HD area. The vegetation communities and soil nutrients changed significantly with increasing soil degradation levels, and Sphingomonas could be used as potential biomarker of degraded alpine wetlands.

The effect of gut microbiota dysbiosis on patients with preeclampsia.

To compare the difference of gut microbiota between preeclampsia (PE) and healthy normal pregnant women, providing new therapeutic strategy for preeclampsia. Forty-one PE patients and 45 age- and pre-pregnancy body mass index- matched healthy controls were enrolled from Nov 2021 to May 2022 in this retrospective case-control study. Fecal microbiota was detected by 16S rRNA gene sequencing, followed by bioinformatics analysis including microbial α diversity, microbial β diversity, and linear discriminant analysis effect size (LEfSe) analysis. Serum inflammatory factors were also detected and compared between the two groups. There were significant differences in Bacteroidetes (2.68% in PE patients vs 11.04% in healthy controls, P < 0.001), Proteobacteria (4.04% in PE patients vs 1.22% in healthy controls, P = 0.041), and Fusobacteria (1.07% in PE patients vs 0.01% in healthy controls, P = 0.042) between the two groups at the phylum level. Microbial α diversity was lower in PE patients than that in healthy controls. In addition, there was significant difference in microbial β diversity between the two groups. LEfSe analysis showed that there are 24 different taxa between the two groups. The levels of proinflammatory factors including serum tumor necrosis factor-α and Interleukin-6 were statistically significant higher in PE patients than those in healthy controls (both P < 0.001), while there were no significant differences in the levels of serum anti-inflammatory factors including Interleukin-4 and Interleukin-10 between the two groups (P = 0.234 and P = 0.096, respectively). PE patients demonstrated gut microbiota disturbances and increasing serum proinflammatory factors, leading to a better understanding of the relationship between the gut microbiota dysbiosis and PE.