Effects Of Wuzi Yanzong Pill Research Articles

The regulatory effect of Wuzi Yanzong pill on UPR signaling pathway in Parkinson's disease mice

Wuzi Yanzong Pill(WYP) has a potential therapeutic effect on Parkinson's Disease(PD) Mice, but the mechanism is not clear. To explore the therapeutic effect of WYP on PD mice and its regulation mechanism on UPR signaling pathway.

Effect of Wuzi Yanzong Pill on oxidative stress response in Parkinson's disease mice

Effect of Wuzi Yanzong Pill on oxidative stress response in Parkinson's disease mice

Effect of Wuziyanzong pill on metabolism of dapoxetine in vivo and in vitro

In vitro incubation of rat liver microsomes with 30 μL of 100 μmol·L−1 dapoxetine and 30 μL of 10, 100, 250, 500, 1000, 2500, or 5000 μg·mL−1 Wuziyanzong pill was performed at 37 °C for 60 min. Dapoxetine concentration was analyzed by high performance liquid chromatography (HPLC). The half maximal inhibitory concentration (IC50) of Wuziyanzong pill on metabolism of dapoxetine was 296.10 μg mL−1in vitro. Twelve SD rats were randomly divided into 2 groups: Control group and Wuziyanzong pill group. The two groups were administrated with 10 mL·kg-1 saline (Control group) or 10 mL·kg-1 Wuziyanzong pill solution (Experimental group, solution contained 200 mg mL-1 Wuziyanzong pill) for 15 consecutive days. Following administration of saline or Wuziyanzong pill on the 15th day, 20 mg kg-1 dapoxetine was administered to all rats. Blood was collected from the tail vein (0.3 mL) at multiple time points, and ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) was used to determine the concentration of dapoxetine and its main metabolites, dapoxetine-N-oxide and desmethyldapoxetine in rats. Pharmacokinetic analysis of dapoxetine showed that area under the concentration-time curve (AUC) and mean maximum plasma concentration (Cmax) of the Wuziyanzong pill group were decreased, while plasma clearance (CLz) was increased compared with control group (P < 0.01). The HPLC method for determination of dapoxetine in vitro was accurate and specific. The UHPLC-MS/MS method established for determination of dapoxetine and its major metabolites in rat plasma was rapid and specific, which met the requirements of pharmacokinetic guidelines. Wuziyanzong pill had a weak inhibitory effect on metabolism of dapoxetine in vitro, but had a very strong induction effect in vivo, suggesting the dosage of dapoxetine should be increased when administered in combination with Wuziyanzong pill.

AB220. Effect of WuziYanzong Pillon Cox7a2 gene and Claudin-11 expression in rats with kidney essence deficiency syndrome

ObjectiveTo observe the effects of WuziYanzong Pill (WYP) on Cox7a2 gene, Claudin-11 expression and to investigate extracellular signal-regulated kinase (ERK) phosphorylation in rat with kidney essence deficiency syndrome.MethodsFive groups of Sprague-Dawley male rats (5 in each group) were given vehicle, multiglycosides of Tripterygium Wilfordii Hook f (GTW) alone (20mg/kg), or GTW followed by WYP (0.5, 1.0, or 2.0 g/kg daily) respectively for 30 days. Cox7a2 mRNA expression in testis was determined by real-time reverse transcriptase polymerase chain reaction. The extracellular signal-regulated kinase1/2 (ERK1/2) phosphorylation level and Claudin-11 expression were assessed by western blot analysis.ResultsGTW induced increased cox7a2 mRNA expression and an activation of ERK as well as a decline of Claudin-11 expression. Compared with GTW group, ERK phosphorylation level in high-dose WYP group decreased greatly with upregulated Claudin-11 expression. Furthermore, high-dose and mid-dose WYP downregulated cox7a2 mRNA levels.ConclusionsWYP protected the impaired spermatogenesis possibly through mediating mitochondrial energy metabolism and blood test is barrier function via ERK pathway.

Effect of Wuziyanzong Pill on sperm quality and calcium ion content in oligoasthenospermia rats

ObjectiveTo study the effect of Wuziyanzong treatment on the sperm quality and content of calcium ions (Ca2+) in oligoasthenospermia rats. MethodsA model of oligoasthenospermia was induced in 50 Sprague Dawley rats by treatment with tripterygium glycosides at 30 mg/kg per day for 8 weeks. They were divided randomly into a model group, a positive group (Huangjingzanyu capsule, 3.01 g/kg), and low, medium and high dose Wuziyanzong treatment groups (2.30, 4.60, 9.20 g/kg crude drug respectively) with 10 in each group. Another 10 rats were used as a control group. The rats in the control and model groups were administered distilled water, while the rats in the remaining groups were administered Wuziyanzong for 30 d. The epididymides were removed, spermatozoa recovered and the sperm density and viability were measured. The spermatozoa were purified and the contents of Ca2+ in the cytoplasm and mitochondria were detected by flow cytometry and atomic absorption spectrometry, respectively. ResultsAfter 8 weeks of treatment with tripterygium glycosides, the sperm density, sperm activity and the Ca2+ content of spermatozoa in the model rats were all significantly decreased compared with the control group (all P<0.05). After 30 d treatment, the sperm density and activity improved and the Ca2+ content of sperm were increased significantly in the medium and high dose Wuziyanzong treatment groups in comparison with the model group (all P<0.05). ConclusionsThe Wuziyanzong treatment increased sperm density, improved sperm viability and enhanced the content of Ca2+ in the sperm cytoplasm and mitochondria in this rat model of oligoasthenospermia.