Medium damping fasteners have the advantages of low cost, easy maintenance, and convenient in gauge adjustment, etc., which are gradually and widely used in metro construction. In order to study the performance of medium damping fasteners in a driving environment, we performed measurement on site about the vertical and lateral displacement of the rail, as well as the vertical acceleration of the rail and the tunnel wall on a straight and curved metro cross-section with medium damping fasteners. By comparing with the results of ordinary fasteners, it can be seen that: (1) The medium damping fastener has a vibration reduction effect on the vertical acceleration level of the tunnel wall in the frequency range of 6.3∼31.5Hz and 50∼80Hz; moreover, it has a vibration reduction effect on the lateral acceleration of the tunnel wall in the frequency range of 5∼31.5 Hz and 50∼80 Hz. (2) The vertical and lateral displacement of the rail and the gauge change with the medium damping fasteners are larger than those of the ordinary fasteners. (3) The maximum Z vibration level measured from the tunnel wall is most suitable for evaluating the source strength of the metro, wherein the source strength of this metro line is 88.42 dB.