Effects Of Various Types Research Articles

Salidroside inhibits chronic myeloid leukemia cell proliferation and induces apoptosis by regulating the miR-140-5p/wnt5a/β-catenin axis.

Salidroside, an active ingredient of Rhodiola rosea, exhibits antitumor effects in various types of cancer. However, the role of salidroside in chronic myeloid leukemia (CML) has not been elucidated. In the presents study, cell viability was assessed by CCK-8 assay, while apoptosis was detected by flow cytometry. Reverse transcription-quantitative PCR analysis was used to examine the expression levels of miR-140-5p in human CML cell lines. The expression levels of apoptosis and cell cycle-associated proteins and of the wnt5a/β-catenin signaling pathway were determined by western blot analysis. Bioinformatic analysis and luciferase reporter assays were employed to investigate the association between miR-140-5p and wnt5a. The results revealed that exposure of CML cells to salidroside (80 µM) inhibited cell proliferation and promoted apoptosis. In addition, salidroside treatment led to the upregulation of miR-140-5p expression. Furthermore, the inhibition of wnt5a/β-catenin signaling pathway and the pro-apoptotic effects induced by salidroside were attenuated by miR-140-5p silencing. Notably, wnt5a was revealed to be a direct target of miR-140-5p. The present findings indicated that salidroside exerted anti-CML effects through regulating miR-140-5p by suppressing the wnt5a/β-catenin signaling pathway. The present study provided evidence of the therapeutic role of salidroside in CML.

The effects of various types of phosphate fertilizers on the environment and their natural activity

In this study, the effect of various types of fertilizers on natural radioactivity levels in agricultural soil was investigated in this research. The activity concentration of 226Ra, 232Th, and 40K in fertilizer samples were determined by using gamma-ray spectroscopy and a high-purity germanium (HPGe) detector to determine the activity concentrations 226Ra, 232Th, and 40K. The activity concentrations for 226Ra, 232Th, and 40K in fertilizers and phosphate raw were (2300-5400) Bq/kg, (9-53) Bq/kg, and (56-3000) Bq/kg, respectively. This research could serve as a baseline data for understanding the effects of fertilizer radiation on human health.

Physiotherapy in the Сomplex Rehabilitation of Children with Anorectal Malformations: Systematic Review

Medical rehabilitation is an integral part of the treatment for surgical patients. Regarding pediatric patients with anorectal malformations, the success of the result of surgical treatment is mainly a correctly selected rehabilitation program, including methods of individual physiotherapy. Disturbances of intestinal transit, such as chronic constipation and anal incontinence, according to the world literature, occur in the study group in up to 30% of cases. According to the social significance of constipation and anal incontinence, the psychological aspects, the disabling component, children with anorectal malformations need early and long-term rehabilitation, adapted to their needs. At the moment, there are regulatory documents describing the principles of medical rehabilitation of children, its main characteristics, however, there are no protocols for the use of physiotherapy methods regarding the manifestations of colon transit disorders. Aim. Analysis of modern literature data on physiotherapeutic methods of rehabilitation of children with anorectal malformations. Material and methods. After determining the research criteria, a basic literature review using Web of Science, PubMed, electronic library was conducted, as a result 186 articles were selected that met the search criteria. After the initial assessment, 10 full-text articles were accepted for the analysis. Results. We have assessed the opportunity and mechanisms of the therapeutic action of sacral stimulation and tibial neuromodulation, the effect of various types of currents and a high-intensity magnetic field on the muscles of the complex, studied the possibility and results of using biofeedback therapy in children with colon transit disorders after operations on the anorectal body area in 421 children in 10 studies. Conclusion. Based on the results of scientific research, a range of physiotherapy methods have been established that are effective in relation to rehabilitation measures in children with anorectal malformations.

Effects of Strength Additives on the Properties of High Bulk Pulp Mold

The bulkier structure would be one of the most important properties of the pulp mold as promising packaging materials. The low-polar solvent mixing at the forming process of wet pulp mold manufacturing resulted in the bulkier pulp mold. But the strength properties of the pulp mold would decreased. In this study, the effects of various types of paper strength additives on the strength of the pulp mold were evaluated depending on the forming condition in order to improving the strength properties of the bulker pulp mold. The dry strength and the wet strength of pulp mold were increased by the addition of the strength additives, but the effects of the additives were relatively lower in case of the low-polar solvent mixing condition. The application of the strength additives resulted in the higher increase in the strength properties of the pulp mold made of ONP than those of the pulp mold made of unbeaten BKP. The dry elongation of the pulp mold made at the low-polar solvent mixing condition showed the higher value of the pulp mold made at the water 100% condition. Although the strength properties of the pulp mold made at the low-polar solvent mixing condition were decreased, the application of the strength additives on the pulp mold could compensate for the reduction in the strength properties of the bulkier pulp mold.

Multitype Damage Detection of Container Using CNN Based on Transfer Learning

Due to the repeated bearing of mechanical operations and natural factors, the container will suffer various types of damage during use. Adopting effective container damage detection methods plays a vital role in prolonging the service life and using function. This paper proposes a multitype damage detection model for containers based on transfer learning and MobileNetV2. In addition, a data set containing nine typical types of container damage is established. To ensure the validity and practicability of the model, we conducted tests and verifications in the actual port environment. The results show that the model can identify multiple types of container damage. Compared with the existing models, the damage detection model proposed in this paper can ensure the identification effect of various types of container damage, which is more suitable for the actual container detection situation. This method can provide a new idea of damage detection for container management in ports.

Podofilox suppresses gastric cancer cell proliferation by regulating cell cycle arrest and the c-Myc/ATG10 axis.

Gastric cancer (GC) is a malignancy for which effective therapeutic drugs are limited. Podofilox exhibits antitumor effects in various types of cancer; however, whether it may inhibit GC growth remains unknown. The aim of the present study was to investigate the role of podofilox in GC. Cell Counting Kit-8, colony formation and cell cycle assays were used to detect the role of podofilox on cellular proliferation and the cell cycle, respectively. A microarray was used to detect the transcriptional changes induced by podofilox in GC cells. The results of the present study demonstrated that podofilox inhibited GC cell proliferation and colony formation. The half maximal inhibitory concentration of podofilox in AGS and HGC-27 cells was 2.327 and 1.981 nM, respectively. In addition, treatment with podofilox induced G0/G1 cell cycle arrest. Molecular analysis based on microarray data demonstrated that podofilox altered the expression levels of genes involved in the cell cycle, c-Myc and p53 signaling. Autophagy-related 10 (ATG10), which was highly expressed in GC tissues, was also downregulated by podofilox, as demonstrated by the results of the microarray analysis and immunoblotting. To determine the involvement of ATG10 in GC, ATG10 was knocked down in GC cells by small interfering RNA, which suppressed the proliferation and colony formation of GC cells compared with those observed in the control-transfected cells. Taken together, the results of the present study suggested that podofilox may inhibit GC cell proliferation by preventing the cell cycle progression and regulating the c-Myc/ATG10 signaling pathway.

Short-Term Physical and Psychological Health Consequences of Induced and Spontaneous Abortion: A Cross-sectional Study

Background: Abortion is an important pregnancy complication with short-term and long-term adverse physical and psychological effects on women. Objectives: The present study aimed to investigate the short-term physical and psychological effects of various types of medical abortion in pregnant women referring to educational and medical centers in Shiraz, Iran. Methods: In this cross-sectional study, among 5176 deliveries by the women referring to Shiraz Educational and Forensic Medicine Centers during a 4-month period, 437 pregnancies ended in abortion and thus were included in this study. A questionnaire was completed for each of the abortions, recording demographic characteristics and midwifery information. As well, the Mississippi Post Traumatic Stress Disorder Scale (M‑PTSD) was filled. Data were analyzed by SPSS software using appropriate statistical tests. Results: The most common physical complications of induced and spontaneous abortion were fever (10.3%) and the need for transfusion of blood products (4.6%). Among the patients who received abortion therapy, 73.1% had moderate stress due to psychological complications. Conclusions: Since the most common complications after abortion are fever, the need for blood transfusion, and bleeding, it is recommended to prepare a protocol to standardize post-abortion care. Most women in both induced and spontaneous abortion groups had moderate levels of stress. Due to the lack of attention to the mental health of the women experiencing abortion, it is necessary for these women to receive adequate supportive counseling, in addition to other medical services, after abortion.

Unsteady MHD free convection flow past a vertical permeable flat plate in a rotating frame of reference with constant heat source and variable thermal boundary condition in a nanofluid

Abstract The problem of unsteady magnetohydrodynamics (MHD) free convection flow of nano-fluid past an oscillation moving vertical semi-infinite permeable flat plate with constant heat source and vertical variable thermal boundary condition in a rotating frame of reference is studied theoretically. The boundary layer has the oscillatory type of solution because slip velocity is assumed to oscillate in time with constant frequency. The governing equations are solved using small perturbation approximation. For this investigation, we use two types of nano-particles in base fluid, namely Cu (copper) and Al2O3 (aluminium oxide). The effect of various types of suspended metallic nano-particles and non-dimensional physical parameters on the fluid flow and heat transfer rate are presented and discussed through the graphs.

Exploring the effects of various types of stressors on the physical and mental health symptoms of police officers in South Korea

This study compared the stress levels and physical and mental health symptoms of patrol officers and emergency dispatch officers in South Korea. We further explored stressors affecting the physical and mental health of police officers. Survey data were obtained from 254 patrol officers and 177 emergency dispatch officers working in Seoul. The average stress level exerted by the stressors was compared between groups, with consideration given to the characteristics of emergency calls. Logistic regression analysis was applied to determine the effects of the stressors on police officers' physical and mental health symptoms. Stress related to organizational management had the highest impact on physical and mental health, and differences in the stressors affecting the two group's physical and mental health were found. The results suggest that strategies reflecting the respective division stressors and characteristics are necessary. Furthermore, police officers' stress levels must be closely monitored and reduced through stress management programs.

Effect of Laparoscopic Sleeve Gastrectomy vs. OAGB on Thyroid Function Tests in Patients with Morbid Obesity and Subclinical or Clinical Hypothyroidism.

As one of the major problems for the health sector, morbid obesity is associated with many hormonal dysfunctions, including thyroid hormone disorders. Few studies have been performed on improving subclinical and clinical hypothyroidism following bariatric surgery. Therefore, we designed and conducted this study to evaluate the effect of various types of bariatric surgery on thyroid hormone levels and to assess the change of levothyroxine requirement in these patients. The data of all patients who underwent either sleeve gastrectomy (SG) or one-anastomosis gastric bypass (OAGB) surgery in the 5years from 2016 to 2020 at our hospital were analyzed retrospectively. From 1486 patients (792 cases of SG and 694 cases of OAGB), 281 patients had preoperative abnormal thyroid function tests: subclinical hypothyroidism (102 patients) and clinical hypothyroidism (179 patients). The T4 level did not change significantly in any of the groups and subgroups. However, the TSH level at the end of the sixth month after SG and OAGB in both groups significantly lowered. Nevertheless, there is no significant difference between the effect of SG and OAGB surgical procedures on the rate of TSH and T4 alterations in both subclinical and clinical hypothyroid patients. In the sixth month after surgery, 49% of clinically hypothyroid patients in the SG group (n = 179) reduced or stopped taking the medication. Whereas bariatric surgery reduces TSH levels, it does not significantly alter T4 levels. Furthermore, there is no apparent difference between subclinical and clinical hypothyroidism and different types of bariatric surgery.

Combination of Melatonin and Zoledronic Acid Suppressed the Giant Cell Tumor of Bone in vitro and in vivo.

Melatonin (Mlt) confers potential antitumor effects in various types of cancer. However, to the best of our knowledge, the role of Mlt in the giant cell tumor of bone (GCTB) remains unknown. Moreover, further research is required to assess whether Mlt can enhance the therapeutic effect of zoledronic acid (Zol), a commonly used anti-GCTB drug. In this research, we investigated the effects of Mlt, Zol, and the combination of these two drugs on GCTB cells’ characteristics, including cell proliferation, apoptosis, osteogenic differentiation, migration, and invasion. The cell counting kit-8 (CCK-8) assay, colony formation assay, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay (TUNEL), alkaline phosphatase (ALP) staining, alizarin red staining (ARS), scratch wound healing assay, and transwell experiment were performed, respectively. Our results showed that Mlt could effectively inhibit the proliferation, migration, and invasion of GCTB cells, as well as promote the apoptosis and osteogenic differentiation of tumor cells. Of note, a stronger antitumor effect was observed when Mlt was combined with Zol treatment. This therapeutic effect might be achieved by inhibiting the activation of both the Hippo and NF-κB pathways. In conclusion, our study suggests that Mlt can be a new treatment for GCTB, which could further enhance the antitumor effect of Zol.

Ozone induces BEL7402 cell apoptosis by increasing reactive oxygen species production and activating JNK.

BackgroundOxidative stress is an important factor in the modulation of both tumorigenesis and anticancer responses. Ozone (O3) is a strong oxidant that causes redox reactions and exerts anticancer effects in various types of cancer cells. However, the pathways involved in O3-induced cell death are not well understood.MethodsIn vitro human hepatocellular carcinoma (HCC) BEL7402 cells were treated with various O3 concentrations to evaluate O3 cytotoxicity by Cell Counting Kit-8 (CCK-8) assay and flow cytometry. The regulatory mechanisms were analyzed by western blot analysis. In vivo, an HCC model was established to evaluate the inhibition of HCC with O3 treatment.ResultsIn vitro cells treated with O3 exhibited a round and small morphology with nuclear shrinkage and fragmentation. The CCK-8 assay confirmed the potent cytotoxic activity of O3 against BEL7402 cells (IC50 value of 5 µg/mL). Acridine orange/ethidium bromide (AO/EB) staining revealed apoptosis of BEL7402 cells after O3 treatment. Flow cytometry analysis showed that S phase cell cycle arrest and apoptosis increased with O3 exposure. In addition, O3 exposure reduced the mitochondrial membrane potential (ΔΨm) and induced reactive oxygen species (ROS) accumulation. Western blot analysis showed that O3 exposure reduced B-cell lymphoma 2 (BCL-2) expression and increased cleaved poly ADP-ribose polymerase (PARP), cytochrome C (Cyt-C), caspase-3, caspase-9, and p-JNK expression. In vivo, treatment with intratumor injection O3 (20 µg/mL) inhibited HCC growth.ConclusionsOverall, our findings showed that O3 induces BEL7402 cell apoptosis via the intrinsic mitochondria-dependent pathway. Therefore, O3 has therapeutic potential for HCC.

Вакцина Гам-КОВИД-Вак (Спутник V) не оказывает негативного влияния на сперматогенез у мужчин

Relevance. During the pandemic and large-scale vaccination against COVID-19, studies of the effect of various types of vaccines on human reproductive function have become particularly important. Vaccination may have a negative impact on the reproductive organs and tissues due to the development of an autoimmune process involving the gonads. Currently, there are some studies on the effect of vaccination against coronavirus infection caused by SARS-CoV2 on the male and female reproductive function. The absence of a negative effect of vaccination on spermatogenesis in men has already been demonstrated in the results of three studies. Objective. To evaluate the effect of the Gam-COVID-Vac (Sputnik V) vaccine on spermatogenesis and the level of antiphospholipid antibodies in men. Materials and methods. The prospective study included 45 men who were vaccinated against COVID-19 with Gam-COVID-Vac (Sputnik V). The criteria for inclusion in the study were the age from 18 to 55 years, the absence of a previous history of COVID-19, a negative RT-PCR result in SARS-CoV-2 testing, negative SARS-CoV-2 IgG results prior to vaccination, and the absence of severe somatic diseases. The patients were examined twice: immediately before vaccination and 90 days after the first dose was injected. We evaluated spermogram parameters, determined the levels of FSH, LH, TSH and total testosterone, and analyzed blood for IgM and IgG antiphospholipid antibodies (aPL) to cardiolipin (aCL), β2-glycoprotein-1 (as2-GP1), annexin V (aAnV), phosphatidylserine (aPS), as well as IgG antibodies to SARS-CoV-2 using enzyme immunoassay. Results. There were no significant changes in the parameters of spermogram and hormone levels before and after vaccination;men with moderate impairment of spermatogenesis (oligoasteno-and/or teratozoospermia) did not show considerable changes either. The level of aPL after vaccination did not increase compared to the baseline level. Besides, there was no correlation between the dynamics of the level of antisperm antibodies, aPL and spermogram parameters. These findings indirectly indicate that there are no changes in the reproductive function associated with this autoimmune factor after vaccination in men. Conclusion. This is one of the first studies that has revealed the effect of the Gam-COVID-Vac vaccine on spermatogenesis, hormonal profile and aPL level in men. The obtained results demonstrate that the Gam-COVID-Vac vaccine does not have a negative effect on the reproductive function in men.

Agmatine: A potential Neurotherapeutic Agent

Agmatine, a natural polyamine disregarded almost for over 100 years, was discovered in year 1910. Almost after a decade, several researches on Agmatine indicated its modulatory action at multiple molecular targets such as, nitric oxide synthesis, neurotransmitter systems, and polyamine metabolism unbolt the new avenues for extensive therapeutic applications which includes neurotrauma and neurodegenerative diseases, antidepressant, cognitive disorders. Agmatine exerts its varied biological characteristics and therapeutic potential in diverse arena. Agmatine has been extensively researched for its neuroprotective effect in various types of neurological diseases, including stroke and trauma brain injury along with Parkinson's disease, Alzheimer's disease, Hypoxia /Ischemia. In the present review we have summarized the therapeutic potential of agmatine as protective and regenerative properties in the CNS.
 Keywords: Agmatine, Neuroprotective, Alzheimer's disease, Parkinson's disease, CNS disorders.

The Role of Vitamin D as a Prognostic Marker in Papillary Thyroid Cancer

Simple SummaryThyroid cancer is the most common endocrine malignancy in the United States and papillary thyroid cancer is by far the most common subtype. Vitamin D has been postulated as a key modulator in several cancer-related pathways, although its contributions to thyroid cancer remain controversial. In this paper, we review the metabolic pathways of vitamin D and explore potential links to cancer-related mechanisms. In addition, we also explore the medical literature related to vitamin D as a prognostic marker for staging in papillary thyroid cancer. The role of vitamin D in modulating several cancer-related pathways has received an increasing amount of attention in the past several years. Previous literature has found an abundance of evidence of vitamin D exerting an anti-proliferative, anti-inflammatory, and pro-differentiation effect in various types of cancers including breast, colon, prostate, and pancreatic cancer. Although the link between vitamin D and thyroid cancer remains controversial, both biochemical evidence and clinical studies have attempted to establish a link between papillary thyroid carcinoma (PTC) and vitamin D status. Furthermore, the use of vitamin D as a prognostic marker has received increased attention, both in regards to clinical outcomes and cancer staging. In this review, we briefly discuss the metabolism and proposed mechanism of action of vitamin D in the context of PTC, and explore links between modulators in the vitamin D pathway and progression of PTC. We provide evidence from both clinical studies as well as molecular studies of metabolic targets, including vitamin D receptor and activating enzymes exerting an effect on PTC tissue, which indicate that vitamin D may play a significant prognostic role in PTC.

Effects of various types of cellulose nanofibers on the physical properties of the CNF-based films

Cellulose-based films using three different types of cellulose nanofibers (CNFs), carboxymethylated CNF (CNFCM), enzyme-treated CNF (CNFENZ), TEMPO-oxidized CNF (CNFTEMPO), and sodium carboxymethyl cellulose (CMC) were prepared using a solution casting method, and their film properties were characterized to test the effect of CNF types. FE-SEM image showed the densely packed fibers in the film with different fiber shapes depending on the CNF types, while the AFM image showed the roughness of the films. The CNFTEMPO film was as transparent as the CMC film, while the other CNF films were translucent. The CNF films also showed different crystalline structures depending on the CNF types, so the film properties were greatly influenced by the cellulose crystallinity index (CI). The CNFENZ film with the highest CI showed higher thermal stability and water vapor barrier properties than the other films. Also, the water vapor permeability of CNF films was significantly lower than the CMC film. The carboxylate and carboxymethyl groups in the CNFTEMPO and CNFCM films increased the tensile strength, which was about two times higher than that of the CNFENZ and CMC films. The CNF film properties depended on the CNF type and were comparable or superior to the CMC film.

Evaluation of filtration effectiveness of various types of facemasks following with different sterilization methods

Due to the ongoing pandemic, various types of facemasks such as certified N-95, non-woven fiber and fabric/textile masks are being used as an essential protective measure to reduce the risk of spread of the SARS-Cov-2 virus. The aerosols size-dependent filtration efficiency and breathing resistance of these masks were tested before and after sterilization by five different methods for two flow rates (20 and 90 L/min) conditions corresponding to regular breathing rate and moderate/strenuous exertion, in the particle size range 0.3–10 µm. Sterilization techniques used here are autoclaving (30 and 60 minutes), dry air oven heating (30 and 60 minutes), ionizing irradiation (15 and 25 kGy), hot water washing with and without detergent and immersing in a 10% concentration of liquid hydrogen peroxide for 30 minutes. Further, the filtration efficiency of each type of masks is also studied with laboratory generated two-order higher aerosol concertation. The certified mask has the most outstanding filtering efficiency among all the other type of facemasks. The ionizing radiation causes a significant reduction in filtration efficacy, so that it is not recommended for sterilization purpose. The best method to sterilize certified N-95 masks without affecting their performance is by using dry air heating with temperature ranging from 70–80 °C. The performance of the cloth and surgical masks is found to be comparable for both flow conditions. As an affordable sterilization method, hot water washing is recommended, which does not deteriorate the fabric masks efficiency and can be used by the common people. The recommended masks for the general people are textile/fabric masks which serves fit for the purpose than non-woven masks because it can help to reduce non-biodegradable waste (facemask) and prevent respiratory droplet transmission. The non-woven mask can be sterilized with dry heat, hot water wash and autoclave.

Carbonated Soft Drinks and Orthodontics: Review of Literature.

This study aims to review the effects of various types of carbonated soft drinks on the behavior of different orthodontic materials as well as on the enamel surface and tooth movement. Articles and books from 1990 to 2020 explaining the effects of carbonated soft drinks on general health and orthodontic materials and tooth movement were electronically searched. The major effects of carbonated soft drinks are explained and discussed in this review. Patients with orthodontic problems must be warned about the side effects of carbonated soft drinks on general and dental health and orthodontic appliances in particular.

Social media marketing for businesses: Organic promotions of web-links on Facebook

The effectiveness of social media marketing is a topic of great interest for researchers as well as marketers. To enrich the literature regarding the effectiveness of various types of posts with a web-link, we designed and conducted an experiment on Facebook (FB). This experiment was conducted in a real business environment, through the FB fanpage of a Polish e-commerce store. The observations were analyzed using multiple linear regression and metrics adapted to this experiment from the literature. The results show that a web-link placed in the comments of an FB post, instead of the caption, is more lucrative. It is also shown that, based on the aims of the campaign, such metrics can give valuable information about the optimal time for posting, as well as the interval between posts.

Effect of Various Types of Sugar Binder on the Physical Properties of Gum Powders Prepared via Fluidized-Bed Agglomeration.

Particle agglomeration of fine gum powders to improve their physical and morphological characteristics is of crucial importance. Changes in the physical properties of guar gum, locust bean gum, and carboxymethyl cellulose powders subjected to fluidized-bed agglomeration with various sugar types as the binder were examined. The agglomerates with sugar binders had much larger particles (D50) and higher porosity (ε) than the corresponding fine gum powders, as confirmed by particle-size-distribution analysis and scanning electron microscopy. In particular, the carboxymethyl cellulose agglomerate exhibited much higher D50 and ε values than the original fine gum powder, with sorbitol as the binder resulting in the highest D50 and ε values. Except for guar gum with sorbitol as the binder, the guar gum and locust bean gum agglomerates with the other sugar binders showed lower Carr index and Hausner ratio values (thus exhibiting better flowability and lower cohesiveness) than the original powders, whereas those of the carboxymethyl cellulose agglomerates were higher. These findings indicate that the physical and structural properties of gum powders can be greatly improved according to the type of gum and sugar solution used in the agglomeration process.