Effects Of Stress Research Articles

The collaboration and competition between indigenous microorganisms and exogenous anaerobic digester sludge in anaerobic treatment of pickled mustard wastewater at different salinities

The highly concentrated pickled mustard wastewater presents significant potential for energy recovery, but the stress effect of high osmotic pressure on cell integrity and activity seriously impedes the methane production by anaerobic microorganisms. The survival ability of indigenous microorganisms (IM) in pickled mustard wastewater supports the establishment of anaerobic treatment. Moreover, inoculation of anaerobic digester sludge is a common start-up strategy. However, the effects of exogenous anaerobic sludge on IM are unclear, especially in hypersaline environment. This research aimed to investigate the influence of exogenous anaerobic sludge on the construction, performance, and microbiota at 3% and 5% salinity. And the research focused on the collaboration and competition between exogenous anaerobic sludge and IM. The neutral community model (which explains the formation and evolution of biological communities) indicated that the interaction between exogenous digester sludge microorganisms and IM dominated community assembly. At 3%, the digester sludge collaborated with IM to increase daily COD reduction and biogas production compared with IM group. However, at 5%, the competitive relationship reduced daily COD reduction and biogas production compared with IM group. This study provides a new perspective for the selection of inoculation strategies for exogenous anaerobic digester sludge under different salinity, in order to realize energy conversion from salinity organic wastewater.

Investigating the efficacy of dapsone in treating sepsis induced by cecal ligation and puncture surgery in male mice.

Sepsis is a life-threatening condition caused by the body's response to an infection. Dapsone is a sulfone with antibiotic properties, and experimental evidence suggests it has significant anti-inflammatory and anti-oxidative stress effects. The objective of this study was to investigate the efficacy of dapsone in mice after CLP (cecal ligation and puncture) surgery, which is a model for inducing sepsis. The study divided animals into five groups: CLP, sham, and three groups receiving different doses of dapsone (0.5, 1, 2mg/kg). Sepsis was induced through CLP surgery, followed by dapsone administration. In each group, half of the mice were used to evaluate levels of various markers and pathological changes at 24h post-CLP, while the other half was used to record the mortality rates within 96h. The results showed that single-dose administration of dapsone at (0.5, 1, 2mg/kg) after CLP surgery improved survival compared to the CLP group. Dapsone was also associated with a significant reduction in pro-inflammatory cytokines TNF-α, IL-1β, IL-6, NO, and MPO, as well as lactate and creatinine serum levels. However, dapsone did not have a significant effect on urea serum levels. In conclusion, the data suggest that dapsone treatment leads to increased survival in septic mice after CLP, and due to its ability to reduce TNF-α, IL-1β, IL-6, MPO,and lactate levels, it has anti-inflammatory effects in sepsis. The sepsis treatment with dapsone in mice protects against inflammation and oxidative stress.

Plant growth, Yield and Leaf Nutritional value of Jute (Corchorus olitorius L.) as Influenced by Banana Peel levels under Salt Stress conditions in Coastal region of Cameroon

In the world, millions of hectares of cultivated land are affected by salt, making salinity a major constraint for plant production. The effects of different levels of banana peel on growth, yield, and chemical changes of jute (Corchorus olitorius L.) under salt stress were examined herein. Response of jute to applications of different levels of banana peel (5, 10, and 15 t ha-1), as well as water irrigation salinity at 50, 100, and 200 mM NaCl were evaluated under greenhouse conditions. The outcome uncovered that salinity caused significant reduction of plant growth and yield parameters, chlorophyll (at 21.6%), LRWC (at 18.9%), P (at 57.1%) and K (at 45.4%) content, while MDA content (at 351.8%), Na (at 266%), soluble proteins (70.2%), total phenolic (at 23.4%) accumulation showed an increase from 0 to 200 mM NaCl without BP application. The banana peel treatments (at 15 t ha-1 under 200 mM NaCl) diminished significantly damaging effects caused by salinity via a reduction in the Na (at 28.4%), total soluble sugars (at 17.8%), total flavonoids (at 20.1%), which enhanced number of leaves per plant (37.1%), plant height (at 19.8%), leave yield (at 41.4%), LRWC (at 12.8%), Mg (at 24.2%) and reduced the MDA content (at 20%), presenting a favorable effect in reducing the oxidative stress that emerged from salt stress. It could be concluded, that the application of 15 t ha-1 of BP was superior in promoting plant growth, yield, and nutritional quality than others under control and in the saline soils in this study. BP at 15 t ha-1 had a more reduced damage of salt stress effect on growth, yield, nutritional value, and use efficiency.

Thriving against the odds: Unveiling the role of melatonin in Okra germination and seedling traits under PEG-induced osmotic stress

Altered environmental conditions subject plants to abiotic stresses like salinity, drought, temperature fluctuations (heat and cold) and exposure to heavy metals. Frequent stresses prompt the plant defense system to consistently adapt. Biostimulants assist plants in refining their defense strategies, allowing more effective responses to the changing environment and challenges. Melatonin, a neurohormone and antioxidant in mammals, has extended its presence to the plant domain due to its pervasive nature as a signaling molecule. Melatonin acts as a bio-stimulatory molecule in confronting stress in plants by regulating its growth and development. The present study attempts to comprehend the role of melatonin in combating drought stress. Okra genotypes namely, CO 4, Arka Anamika, Arka Abhay and Arka Nikita were subjected to PEG-induced osmotic stress (15 % PEG) in Petri plates supplemented with different doses of melatonin (50, 100, 150, 200, 250 µM). The seedling response varied with respect to genotypes in a dose-dependent manner. Among the genotypes, Arka Anamika performed better followed by Arka Nikita in terms of seedlings traits recorded. Higher concentrations of melatonin exhibited an inhibitory effect on seed germination and seedling growth parameters. However, priming okra seeds with 100 µM of melatonin showed promising results in mitigating the adverse effect of osmotic stress by promoting seed germination and seedling characteristics.

Experimental study on the creep performance of Grade 8.8 and 10.9 high-strength bolts at elevated temperatures

High-strength bolts are widely used in beam-column connections which are typically subjected to significant pretension and experience high stress levels. Under fire conditions, high-strength bolts may exhibit noticeable creep deformation due to the combined effects of high stress and high temperature, which may result in significant deformation of the connection, thereby threatening the stability of the structure. This paper reports an experimental study on the creep behavior of Grade 8.8 and 10.9 high-strength bolts at elevated temperatures. A series of tensile tests are conducted on high-strength bolts at elevated temperatures, and varying stress ratios are set based on the yield strength measured form the tensile tests. Test results indicate that the temperature has significant influence on the mechanical properties of Grade 8.8 and 10.9 high-strength bolts. Through the entire temperature range, current specifications significantly overestimate the yield strength of high-strength bolts at high temperatures. At the same temperature, the development of creep strain over time is positively related to the stress ratio. An increase in stress ratio from 0.4 to 0.6 or 0.8 results in a maximum 7.8-time increase in creep strain. The temperature of 500℃ can be regarded as the critical temperature at which the creep effects of Grade 8.8 and Grade 10.9 high-strength bolts should be considered. The mechanical property prediction equations and a modified creep model for Grade 8.8 and 10.9 high-strength bolts were developed, which have high reliability in predicting the high-temperature mechanical properties and creep behavior of high-strength bolts.

Seed biopriming with Parachlorella, Bacillus subtilis, and Trichoderma harzianum alleviates the effects of salinity in soybean

BackgroundSeed conditioning with bioinputs (biopriming) offers a promising and sustainable alternative to mitigate the adverse effects of salt stress on soybeans. This study aims to evaluate the potential of isolated or combined biopriming using microalgae and different microorganisms in alleviating salinity-induced stress in soybeans in early-stage.MethodsSeeds were subjected to five biopriming treatments: Parachlorella sp. microalgae culture, Bacillus subtilis, Trichoderma harzianum, Parachlorella sp. + B. subtilis, and Parachlorella sp. + T. harzianum, along with a control group without biopriming. Subsequently, the seeds were exposed to two conditions: (i) control (0.0 MPa), and (ii) salinity induced by NaCl (-0.8 MPa). Germination, photochemical indicators, and seedling performance were assessed.ResultsSalinity impaired root protrusion and seed physiology, resulting in a high percentage of abnormal seedlings, thus creating a stressful condition. However, biopriming alleviated the negative effects of salinity, particularly with T. harzianum, Parachlorella sp. + B. subtilis, and Parachlorella sp. + T. harzianum, which led to high germination rates and normal seedlings. All biopriming treatments, especially the combined ones, reduced the suppression of non-photochemical quenching, thereby enhancing the maximum yield of photosystem II. Seedlings under salt stress without biopriming exhibited short lengths and low fresh and dry mass, whereas those bioprimed with Parachlorella sp. + B. subtilis and Parachlorella sp. + T. harzianum showed significantly higher values.ConclusionSeed biopriming, especially with Parachlorella sp. microalgae culture combined with B. subtilis or T. harzianum, effectively alleviates the stressful effects of salinity on germination and early-stage growth seedling of soybeans.

COVID-19 Pandemic-Related Stressors, Distress, and Bodily Pain in Native Americans: Results from the Oklahoma Study of Native American Pain Risk.

The COVID-19 pandemic disproportionately impacted minoritized individuals. This study examined the relationships between pandemic-related stressors/distress and bodily pain in 79 Native American (NA) and 101 non-Hispanic White (NHW) participants from the Oklahoma Study of Native American Pain Risk. Online surveys were administered in May/June 2020 (wave 1), March/April 2021 (wave 2), and Sept/Oct 2021 (wave 3). Pandemic-related stressors (e.g., resource loss and added responsibilities) and distress were assessed from a custom-built questionnaire. Bodily pain was assessed from pain items on the Patient Health Questionnaire-15 (PHQ-15). The results indicate NAs and NHWs reported similar pandemic-related stressors and distress at wave 1, which remained at similar levels across all waves in NHWs. By contrast, stressors and distress increased in NAs at waves 2 and 3. Moreover, bodily pain was higher in NAs than NHWs across all waves. Regression-based multilevel analyses predicting bodily pain found that NHWs with more pandemic-related stressors/distress experienced more bodily pain, but stress/distress did not predict bodily pain in NAs. Findings demonstrated that NAs experienced more bodily pain and pandemic-related stressors/distress than NHWs. However, pandemic-related stressors/distress did not further exacerbate NA pain as observed in NHWs. This implies NAs may have demonstrated resiliency that buffered the pronociceptive effects of pandemic-related stress.

Hydrogen behavior and microstructural evolution in flexible IGZO thin films under stress

In this study, we investigated the effect of mechanical stress on hydrogen diffusion in flexible amorphous InGaZnO (a-IGZO) thin films and the resulting microstructural changes. The cyclic bending test under different curvature radii (R) revealed significant morphological evolution and bond state changes in IGZO thin films. As the curvature radius decreases from 20 mm to 5 mm, the surface of the sample gradually becomes rough and cracks appear. Simultaneously, changes in nanoscale topological structure and chemical composition exhibit stronger hydrogen diffusion and structural relaxation: The oxygen-hydrogen (O-H) bond content increased from 19 % to 55 %, while the metal-oxygen (M − O) bond content decreased from 50 % to 28 %. The M − H content increased, and In-H related structures underwent transformation. The radius of gyration (Rg) increasing from 1.652 nm to 1.812 nm. These results provide quantitative insights into the stability and performance of IGZO-based flexible electronic devices under mechanical deformation.

PTGS2/GRP78 Activation Triggers Endoplasmic Reticulum Stress Leading to Lipid Metabolism Disruption and Cell Apoptosis, Exacerbating Damage in Bovine Mastitis

Lipoteichoic acid (LTA), an organic acid of Gram-positive bacteria, is closely related to mastitis in dairy cows. This study evaluates the effect of LTA-induced endoplasmic reticulum stress (ER stress) in vitro using MAC-T (mammary epithelial cells) and in dairy cows with mastitis. LTA stimulation significantly increases ER stress and apoptosis-related factors in MAC-T. Further analysis suggests that the increase in ER stress may be associated with interactions involving PTGS2 and GRP78. Protein structural studies indicate a strong interaction between PTGS2 and GRP78. Lipidomics results further demonstrate that LTA disrupts lipid balance in MAC-T cells, affecting lipid metabolism in the endoplasmic reticulum, including PC, PE, TAG, and DAG, thereby exacerbating inflammation and ER stress. In dairy cows with mastitis caused by Gram-positive bacterial infection, damaged epithelial cells, inflammatory cell infiltration, and apoptotic vesicles are observed in affected tissues. In contrast, tissues from healthy cows exhibit regular epithelial cells without inflammatory cells or apoptotic vesicles. Furthermore, a significant ER stress and apoptosis increase is observed in mastitis tissues. This study demonstrates the close association between LTA-induced cell damage and ER stress, contributing to understanding the mechanisms underlying LTA-induced damage and supporting strategies for mastitis prevention and control in dairy cows.

Impact of Water Temperature on Seedling Quality Parameters in Lactuca sativa L., Solanum lycopersicum L., and Brassica oleracea var. gongylodes L.

Heat stress represents a significant challenge to global agricultural production, with particular emphasis on air temperature stress. Despite considerable attention to this issue, limited information is available regarding the impact of irrigation water temperature on the quality of vegetable crops. In this study, kohlrabi, tomato, and lettuce were subjected to three distinct irrigation temperatures: 17 °C, 24 °C, and 34 °C. A variety of parameters were measured for the three vegetables, including seedling height, relative chlorophyll content (SPAD), mass of the green part (FW), mass of roots (FW), dry weight (DW) of the green part, DW of roots, and leaf area. The results indicated a significant decrease in oxygen (O2) content with rising water temperature, with a 20.8% reduction at 34 °C compared to 17 °C. Notably, the highest temperature of 34 °C exerted the most positive influence on the studied parameters, particularly evident in kohlrabi and tomato. This study addresses a critical knowledge gap by elucidating the impact of irrigation water temperature on the growth and development of vegetable seedlings. The findings presented here lay the groundwork for further investigations into the effects of heat stress on agricultural practices.

Detrimental effects of abiotic stress on wheat and its management techniques

Detrimental effects of abiotic stress on wheat and its management techniques

Enhancing farmed fish welfare: Evaluating the effectiveness of plant-based stress mitigating agents as sedatives in sea bass (Dicentrarchus labrax) following intraperitoneal vaccination

The effectiveness of vaccines may be compromised by the stress response induced by intraperitoneal/intramuscular (IP/IM) vaccination due to an intimate interaction between the neuroendocrine and immune systems. Essential oils (EOs), known for their antibacterial, antioxidant, and sedative properties, are potential candidates to mitigate this stress response. This study investigates the short-term sedative effect of two essential oil-based products, FishEase-C (FEC) and FishEase-L (FEL) in sea bass prior to intraperitoneal vaccination (IP). Physiological stress indicators (plasma cortisol, glucose, lactate) increased 1-h post-vaccination (hpv) as expected. Cortisol remained elevated up to 24 hpv in the N.P. vaccine and FEC + vaccine groups but decreased with FEL treatment (FEL + vaccine group). However, FEC at the tested concentration appeared to induce stress. The transcription of stress (gr, hsp70, cox2), immune (il1β, il6, tnfα), and antioxidant (gpx, sod, catalase) genes confirmed the vaccination-induced stress response, with mc2r transcription indicating increased cortisol production in vaccinated groups (N.P. vaccine and FEC + vaccine). FEL reduced stress at both physiological (e.g., cortisol) and transcriptional levels (e.g., hsp70, cox2 and il6) at either 1 or 24 hpv. It is worth noting that, from an inflammatory perspective, there was a big difference between tissues in terms of magnitude and pattern (treatment and time effects). The brain was more resistant to inflammation, while the head kidney and spleen showed heightened il1β expression (860-2100-fold). These findings support the use of FEL as a sedative before IP/IM vaccination in sea bass.

Effect of UV-B stress on olive (Olea europaea L.) pollen tubes: A study of callose plug deposition and male germ unit integrity.

While UV-B radiation is beneficial to plant growth, it can also cause adverse effects. The pollen tube, a key component of plant reproduction with a tip growth mechanism, is an excellent cellular model for understanding how environmental stressors such as UV-B radiation affect plant cell growth. This research investigated the effect of UV-B on olive pollen both before and after germination. Pollen grains were hydrated and exposed to UV-B radiation for 1h. Pollen tube germination was then evaluated 4 and 24h after exposure. To study the effect of UV-B radiation on developing pollen tubes, pollen was germinated for 4h prior to 1h of UV-B exposure. Pollen tube growth was evaluated by assessing the distribution of cell wall components, the distance between callose plugs and nuclei, and the distance between the male germ unit and the pollen tube tip. We also examined the accumulation of callose synthase. The results showed that UV-B radiation significantly inhibited the growth of pollen tubes, thereby preventing successful fertilization. The effect of UV-B exposure on pollen tube growth was mainly due to the alteration of position of callose plugs and the level of callose synthase in the pollen tube, potentially affecting its growth. In addition, UV-B radiation affected the movement and integrity of the male germ unit, a critical element for successful fertilization. This research sheds light on how UV-B radiation affects the growth of pollen tubes and highlights the need for further research into the effects of UV-B radiation on plant cells and plant reproduction.

Enhancing the Photosynthetic and Yield Performance of Rice in Saline Soil by Foliar-Applying Cost-Effective Compounds as Sources of Carbon Dioxide and Potassium

Although rice is highly sensitive to salinity, it is considered one of the best crops to grow in salt-affected mudflat soils to alleviate the salinity problem. Applying chemical compounds for an increase in leaf CO2 and nutrient levels can help mitigate the negative impact of salinity on plants in a cost-effective manner. To identify the benefits of using lithovit (Liv), ethanol (Eth), and potassium carbonate (KC) as a source of CO2 and K to enhance rice production in salt-affected soils, a field study was conducted to assess the effects of these compounds on the agro-physiological parameters of two rice genotypes (Giza178 and Giza179) in saline soils. The compounds were applied as a foliar spray at a concentration of 30 mM each before and after the heading growth stage. The results indicated that the genotype, application time, compounds, and their potential two-way interactions significantly influenced all agro-physiological parameters, with only a few exceptions. The genotype Giza 179 exhibited higher pigment contents, photosynthetic capacity, relative water content (RWC), grain yield, and most yield components compared to Giza 178, with increases ranging from 2.1% to 37.9%. Foliar application of different compounds resulted in a 9.7–37.9% increase in various parameters and a 34.6–43.2% decrease in the number of unfilled grains (NUFG) per panicle compared to untreated treatment. Foliar application of different compounds before heading resulted in an increase in various parameters by 4.8–16.1% and a decrease in the NUFG per panicle by 22.9% compared to those applied after heading. Heatmap clustering analysis revealed that foliar application of Liv before heading was the most effective treatment in enhancing various parameters for both genotypes and mitigating the negative effects of salinity stress on the NUFG. This was followed by Eth and KC before heading for Giza 179. Applying Eth and KC to the leaves after heading had a moderate positive impact on most parameters for Giza 179, outperforming the application after heading for Giza 178. Overall, our findings indicate that spraying readily available compounds that elevate CO2 and K levels in rice leaves can help alleviate the negative impacts of salt stress and improve rice production in salt-affected soils in a cost-effective manner.

Oxytocin Protects Against Corticosterone-Induced DA Dysfunction: An Involvement of the PKA/CREB Pathway

Chronic stress disrupts dopamine (DA) transmission, adversely affecting mood and contribution to neuropsychiatric disorders like ADHD, autism, schizophrenia, anxiety, depression, and drug addiction. The neuropeptide oxytocin (OXT) plays a key role in social cognition, bonding, attachment, and parenting behaviors. In addition, OXT can modulate the activity of the HPA axis, counteracting the effects of stress, and alleviating fear and anxiety. However, whether OXT can mitigate stress-induced DA dysfunction and the underlying mechanisms remains unclear. This study investigated the neuroprotective effects of OXT on corticosterone (CORT) induced DA dysfunction in the neuroblastoma cell line SH-SY5Y. The results revealed that CORT decreases the levels of intracellular signaling molecules associated with DA function, including phosphorylated tyrosine hydroxylase (pTH), phosphorylated cAMP response element-binding protein (pCREB), and protein kinase A (PKA). Interestingly, pretreatment with OXT mitigated CORT-induced DA dysfunction through its potent PKA activator properties. In addition, the neuroprotective effect of OXT was abolished by atosiban (an OXT receptor antagonist) or H89 (a PKA inhibitor). Our results suggest that OXT protects dopaminergic neuroblastoma cells from CORT-induced DA dysfunction, potentially through the involvement of oxytocin receptors and the PKA/CREB signaling pathway. These findings contribute to the understanding of the neurobiological mechanisms underlying stress resilience and highlight potential pathways for developing targeted treatments that leverage the neuroprotective properties of OXT to address disorders characterized by DA dysregulation and impaired stress responses.

Impact of gibberellic acid GA3, quantum dot biochar, and rhizosphere bacteria on fenugreek plant growth and stress responses under lead stress

Lead (Pb) is a stress that can cause problems with several aspects of a plant’s metabolism, potentially impeding the plant’s ability to grow and develop. The use of gibberellic acid (GA3), quantum dot biochar (QDBC), and rhizobacteria (RB) can be effective methods to overcome this problem. Gibberellic acid is a crucial plant hormone that regulates plant growth, cell division, tissue differentiation, flowering, photosynthesis, and transpiration rate. It also significantly impacts crop resilience to stress, affecting plant morphology, enzymatic activity, and physiology. Biochar, a soil supplement, enhances plant development soil health, and reduces stress effects. Due to its large surface area and porosity, it increases soil water-holding capacity, nutrient retention, and microbial activity. Quantum dots, semiconductor nanoparticles, have been proposed as a potential method to alleviate plant stress by acting as antioxidants, reducing oxidative stress, and controlling nutrient and growth regulators. Rhizobacteria, soil bacteria in plant roots, stimulate plant growth, nutrient absorption, and harvesting capacity. They produce phytohormones, increase mineral and nitrogen accessibility, and can induce systemic resistance (ISR), affecting plant defense. This study investigates the effects of combining GA3, QDBC, and RB as amendments to fenugreek, both with 500 Pb stress and without Pb stress. Treatments (control, 0.25 GA3mg/L-QDBC, 0.5 GA3mg/L-QDBC, 0.25 GA3mg/L-QDBC + RB, and 0.5 GA3mg/L-QDBC + RB) were applied in six replications using a completely randomized design. Results demonstrate that the combination of 0.5 GA3mg/L-QDBC + RB with 500 Pb stress led to significant enhancements in fenugreek shoot fresh weight (15.62%), root fresh weight (73.53%), shoot dry weight (24.00%), and root dry weight (36.53%) compared to the control. Additionally, there were notable improvements in chlorophyll a (57.23%), chlorophyll b (19.21%), and total chlorophyll (36.23%) compared to the control under Pb stress, also showing the potential of 0.5 GA3mg/L-QDBC + RB with 500 Pb stress. The study suggests that combining 0.5 GA3mg/L-QDBC + RB with 500 Pb stress can effectively mitigate Pb stress in fenugreek.

Dynamic study on the anodic dissolution of 304 stainless steel with elastic stress in sodium chloride solution

Effects of elastic stresses on the anodic dissolution of 304 stainless steel (SS) in 0.5 mol·dm−3 NaCl solution have been investigated by potentiodynamic polarization technique, scanning electron microscopy (SEM) and digital holography (DH). From the polarization curves, the SEM morphologies and the phase reconstruction maps, the effects of tensile and compressive stresses on the anodic dissolution of 304 SS have been analyzed. The tensile stresses and the large compressive stresses promote the anodic dissolution and increase the pitting susceptibility of 304 SS. However, the small compressive stress inhibits the anodic dissolution and decreases the pitting susceptibility of 304 SS. In this study, DH was used to observe the surface reactivity distribution of both the tensile and compressive stressed regions of the same sample. The conclusions obtained from the in-situ observations with DH are consistent with those drawn from the electrochemical measurements and the surface morphologies.

Molecular and cellular markers in chickens exposed to stressors and the ability of feed supplements to overcome negative effects – a review

Abstract Performance of chickens is negatively impacted by stressors such as heat stress, infection with Campylobacter jejuni, the mycotoxins – aflatoxin B1 and deoxynivalenol, the glucocorticoid dexethasone, insecticides and Eimeria tenella. Effects of stressors include reduced gastrointestinal function and induction of inflammation. Stressors influence the following molecular and cellular structures and functions: gastro-intestinal integrity, villus height, crypt depth, mast cell number, expression of pro-inflammatory cytokines, concentrations of leukocytes, and antioxidant status. In multiple studies, adverse effects of multiple stressors were overcome partly by supplementation. The utility of these parameters is discussed of these are considered and recommendations/guidelines for future research made.

A Comprehensive Analysis of the 9-Cis Epoxy Carotenoid Dioxygenase Gene Family and Their Responses to Salt Stress in Hordeum vulgare L.

Barley (Hordeum vulgare L.) is among the earliest crops to be cultivated and is also considered a crucial staple crop. Nevertheless, the negative effects of abiotic stress on both the quality and productivity of barley are significant. Nine-cis-epoxycarotenoid dioxygenases (NCEDs) are rate-limiting enzymes in plants that cleave carotenoids and produce abscisic acid (ABA). The poor utilization of barley NCEDs in stress-resistant genetic breeding is due to the lack of appropriate information about their potential function in abiotic stress. The current study revealed five NCED genes in the barley genome (HvNCED1—HvNCED5), which are distributed unevenly on barley chromosomes. The PF03055 domain is present in all HvNCEDs, and they encode 413~643 amino acids. Phylogenetic analysis showed that NCED genes were categorized into three distinct clades, confirming the homology of NCED genes between H. vulgare L., Arabidopsis thaliana L., and Oryza sativa L. Expression analysis revealed that HvNCED1 is significantly upregulated under high salt stress, indicating its potential role in enhancing salt tolerance. In contrast, HvNCED3 and HvNCED4 exhibited downregulation, suggesting a complex regulatory mechanism in response to varying salt stress levels. These findings will enhance our comprehension of the genetic composition and evolutionary development of the HvNCED gene family and provide a basis for future research on their role in response to salt-induced stress.

Effect of elevated temperature and water stress on seed germination of the Himalayan medicinal herb Aconitum spicatum

Effect of elevated temperature and water stress on seed germination of the Himalayan medicinal herb <i>Aconitum spicatum</i>