The effect of loading conditions on short crack propagation behaviours in cast AlSi alloys was investigated by a combination of photo-microscopy monitoring and post mortem analysis. The findings suggest that the path of short cracks is minimally influenced by loading conditions, whereas loading conditions significantly affect the fatigue crack growth rate (FCGR) and its statistical characteristics. Crack closure effect is observed under zero and negative stress ratio conditions. Roughness-induced crack closure occurs due to the coarse grain microstructure of cast AlSi alloys and the Stage-I mode growth of cracks within grains in the microstructural short crack regime. The high level of plasticity around the crack tip under negative stress ratio conditions has a significant impact on FCGR, resulting in a negative crack opening stress. When the stress ratio exceeds approximately 0.3, short cracks exhibit no crack closure. The load-level effect is observed only at high load conditions with negative stress ratios. Finally, a probabilistic short crack growth model was established using a Bayesian linear regression model to analyse the effect of stress ratio on the uncertainty of FCGR. After considering the crack closure effect, the difference in distributions of FCGR model parameters decreases significantly among different stress ratio conditions.