Effect Of Storage Temperature Research Articles

The protective role of shell, packaging technique and storage temperature in lipid oxidation in walnuts of different varieties

In this study, the effects of different packaging techniques (normal and nitrogen atmosphere, and vacuum packaging) and different storage temperatures (4 and 20 °C) on lipid oxidation in in-shell and kernel walnuts of ‘Chandler’ and ‘Sebin’ varieties stored for 12 months were investigated. Free fatty acid, peroxide, p-anisidine, conjugated diene and triene values, hexanal contents, and fatty acid compositions of the walnuts were periodically determined during storage. Free fatty acid values of the samples stored at 20 °C were higher than those stored at 4 °C. Peroxide values of all samples fluctuated during storage but the values determined at the end were higher than their initial values. The highest hexanal and p-anisidine values were observed in the kernels packaged under normal atmosphere and stored at 20 °C. No significant changes were observed in conjugated triene values of the samples. Fatty acid composition was most stable in in-shell walnuts stored at 4 °C. Hexanal contents of the samples stored at 4 °C and the ones packaged under nitrogen and vacuum were remarkably lower than those of the samples packaged under air and stored at 20 °C. As a result, the oxidation reactions were strongly limited in in-shell walnuts of both varieties when stored at 4 °C for 12 months. It was determined that the factors affecting the oxidation reactions in walnuts were in a sequence of storage temperature > presence of the shell > variety > packaging technique.

The effect of storage temperature and time on the quality traits and evaluation of fatty acid profile of backyard produced eggs

The effect of storage temperature and time on the quality traits and evaluation of fatty acid profile of backyard produced eggs

Effect of temperature and packaging materials on the shelf-life stability and in vitro properties of microencapsulated and spray-dried synbiotic legume-based instant beverage powder

The aim of the work was to investigate the effect of different packaging materials and storage temperatures on the stability and in vitro properties of microencapsulated spray-dried synbiotic kidney-mung bean beverage (KMB) powder.

Temperature and Preparation Time Intervals on Survival of Opisthorchis viverrini Metacercariae in Pickled Fish (Pla‐som)

This study involved two series of independent trials that investigated the storage temperature and time of raw fish to eliminate Opisthorchis viverrini metacercarial infection in Thai pickled fish. A total of 330 healthy silver barb fishes (30‐day posthatch) were infected with O. viverrini cercariae at an inoculation ratio of 50 cercariae/fish. After infection, the fish was reared for 3 months. The first trial was to evaluate the effect of fish storage temperature in the recovery from O. viverrini metacercariae. The infected fishes were randomly divided into five groups and stored at −20°C, 2°C, 4°C, and 8°C or room temperature (as a control) for 24 h. The results showed that at a storage temperature of −20°C, the fish initially had a reduced recovery rate from O. viverrini metacercariae. The second trial investigated the storage time for the recovery of infected fish stored at −20°C for 18, 24, 36, 48, or 60 h and used room temperature as a control group. The results revealed that storing infected fish at −20°C for 48 and 60 h had the lowest recovery rate from O. viverrini metacercariae at 0.00%. In conclusion, storing infected fish at a temperature of −20°C for a period of 48 h or longer could restrict the O. viverrini metacercaria recovery rate. These results were recommended as suitable conditions for the preparation of Pla‐som to avoid O. viverrini metacercaria contamination.

Effect of Storage Density and Temperature on the Survival and Fatty Acid Profiles of Cyclopoid Copepods

Effect of Storage Density and Temperature on the Survival and Fatty Acid Profiles of Cyclopoid Copepods

Impact of Storage on Nondestructive Detectability of Codling Moth Infestation in Apples

Highlights Codling moth responds differently to low temperature conditions during the supply chain. The length of storage and temperature determine the sensitivity of the codling moth to detection. Hyperspectral imaging sensor detectability of codling moth increased with temperature. Abstract. Different conditions during cold storage of codling moth (CM)-infested apples lead to different infestation levels, which affect overall product quality. In this study, the effects of postharvest storage duration (up to 20 weeks) and temperature (0°C, 4°C, and 10°C) on the detectability of CM-infested apples were investigated using the near-infrared (NIR) hyperspectral imaging (HSI) method (900–1700 nm). Fresh organic Gala apples were obtained directly from a commercial market and stored in a controlled environmental chamber at three temperatures for 20 weeks in two groups: control and CM-infested samples. Every four weeks, NIR hyperspectral images in reflectance mode were acquired directly for each set of samples. Machine learning models for the classification of CM-infested apples were developed based on the HSI data. The results revealed that storage duration and temperature had a significant effect on the performance of the classification models in the detection of CM-infested and control apples. Overall, the best classification rates were obtained for apples stored for 16 weeks, with accuracies of 97%, 94%, and 100% at storage temperatures of 0°C, 4°C, and 10°C, respectively. This study is critical for determining the effectiveness of HSI as a nondestructive method for sorting apples into classes based on CM infestation when stored under different conditions and duration, as in this study. Keywords: Apples, Codling moth, Detectability, Machine learning, Nondestructive method.

A review of storage temperature and relative humidity effects on shelf life and quality of mango (Mangifera indica L.) fruit and implications for nutrition insecurity in Ethiopia

A review of storage temperature and relative humidity effects on shelf life and quality of mango (<i>Mangifera indica</i> L.) fruit and implications for nutrition insecurity in Ethiopia

Physical changes in hen eggs stored at different temperatures

Eggs are among the most nutritious foods, but they are perishable. Immediately after they are laid, ageing processes begin in shell eggs, altering their chemical, physical and functional properties. This study aimed to determine the effect of storage temperature on the shelf life of hen eggs according to changes in some important physical properties (weight, pH, Haugh Unit, Albumen Index, and Yolk Index). The studied temperatures were selected as possible refrigerator and room temperatures (10, 20 and 30°C). It was revealed that temperature significantly influenced egg quality, with the most significant effects observed in the Haugh unit and pH due to weight changes. The highest quality loss occurred at 30°C. The activation energies for each quality parameter were determined using the Arrhenius equation. The pH of the fresh egg was 7.6 ±0.1 and increased to over 8.5 – 9.0 during storage, depending on the temperature. Haugh unit, albumen index, and yolk index decreased, and the area of both yolk and albumen increased during storage.

Viability and integrity of Pinus densiflora seeds stored for 20years at three different temperatures.

Storage temperature is one of the most important factors determining seed longevity in the genebank. This study aimed to investigate the effect of storage temperature on the seed viability and physiological integrity after a 20-year storage period of Pinus densiflora, a tree species of ecological and economic significance in South Korea. To this end, seeds were collected and stored dry for 20years at -18°C, 4°C and 25°C. Germination tests were conducted to assess seed viability and vigour, electrolyte leakage analysis was performed to assess cell membrane integrity, and carbohydrate analysis was conducted to assess metabolic integrity during germination. The results revealed that over 20years, seeds stored at -18°C maintained a high germination percentage (GP; 89%), comparable to initial GP (91%), whilst those stored at 4°C exhibited a decline in GP (44%) along with a decrease in vigour. Seeds stored at 25°C lost their viability entirely. Electrical conductivity of the leachate and leakage of inorganic compounds and soluble sugars were higher with elevated storage temperature, indicating increased imbibition damage. Additionally, changes in carbohydrate content during germination revealed that the loss of viability according to storage temperature is associated with reduced storage reserve utilization and altered carbohydrate metabolism during germination. These results enhance our understanding of the effect of seed storage temperature on longevity and physiological changes of aging in the genebank, serving as a reference for establishing conservation strategies for Pinus densiflora.

Effects of storage temperature and pre-treatments on seed germination of Primula mallophylla and P. sertulum (Primulaceae), two narrowly-distributed endemic species from southwest China

Primula mallophylla and P. sertulum are wild ornamental species of south-west China, but the responses of their seeds to storage have not been determined. When seeds were dry-stored for 24 months at room temperature (RT) (ca. 21°C) and at 4°C, germination increased during the first six months, but then decreased over the following 18 months. The decrease in germination was slower for seeds stored at 4°C than for those stored at RT. When seeds of these two species were dry-stored at -20°C for 24 months, germination percentage was significantly higher than that of the control seeds by the sixth month; the high germination percentages persisted at 24 months. In addition, pre-germination treatments (i.e., immersion in 100 μmol L -1 GA3 for 24 hours, cold stratification for 30 days or both) improved the final germination and reduced mean germination time of P. mallophylla seeds stored for 12 months at RT, but not those of P. sertulum. Taken together, storing seeds at -20°C is feasible and convenient for both primrose species; thus, it is possible to safely store seeds for future research and conservation.

Effect of Pasteurization and Storage Temperatures on the Quality of Carrot Juices

Effect of Pasteurization and Storage Temperatures on the Quality of Carrot Juices

Effect of postharvest treatments and storage temperature on the physiological, nutritional, and shelf-life of broccoli (Brassica oleracea) microgreens

Microgreens are categorized in the class of ‘super foods’ due to their high content of phytochemical and bioactive compounds. Rapid senescence and short life span are the restrictive parameters for the industrial production and marketing of these immature greens. Hence, there is a need to improve the production and storage conditions of microgreens to magnify postharvest quality, life span, and safety of microgreens, thereby emerging markets. To prolong their shelf life, effect of different postharvest treatments (MAP (modified Atmospheric Packaging) and GRAS chemical treatments) and storage temperature on physiological, nutritional and shelf life of broccoli (Brassica oleracea) microgreens studied. The effect of ascorbic acid + citric acid (0.25 % w/v each) along with MAP conditions (15 % CO2 + 5 % O2 + balanced N2) stored at 5 °C was used for post-harvest quality evaluation of broccoli microgreens. Microgreens treated with an organic acid in high CO2 conditions retained significantly (p < 0.05) higher bioactive compounds in terms of total phenols (1247.68 mg GAE/100 g FW), flavonoids (56.9 mg QE/100 g FW), and chlorophyll (42.12 mg/100 g FW) with better antioxidant activity (44.78 % inhibition). In addition, MAP conditions in the package significantly (p < 0.05) suppressed the weight loss that helped to retain better firmness. The combined effect of organic acids and MAP conditions significantly (p < 0.05) reduced the microbial load with better color quality and higher overall acceptability. The results indicate that post-harvest treatment of ascorbic acid + citric acid along with MAP conditions could be the practical approach to enhance the shelf life of microgreens up to 12 days while maintaining the nutritional quality, to provide avenues for better marketability and commercial gains.

The effect of different layer genotypes raised in the Free-range system on egg quality storage at different temperatures

The aim of this study was to investigate the effect of stored eggs obtained from different layer genotypes raised in a Free-range system on egg quality at different storage temperatures. Lohmann Brown, Lohmann Sandy, and ATAK-S were used as layer genotypes in the study. The study was performed in a 3x2 factorial design with three genotypes and two storage temperatures. A total of 300 table eggs were used in the study. Egg quality analysis was carried out on 100 eggs from each genotype. Eggs were stored in refrigerator (4±2°C) and at room temperature (22±2°C) for 28 days. Egg quality was determined in 10 eggs from each group after 0, 7, 14, 21, and 28 days of storage, and the effects of temperature, genotype, and temperature x genotype interaction were determined. In the study, the effects of genotype and storage temperature on egg weight loss, Haugh unit, yolk index, and albumen pH were determined to be significant (P&amp;lt;0.05). However, the interaction effects of storage temperature x genotype were not statistically significant for any period of storage on egg quality characteristics such as Haugh unit and albumen pH. As a result, it was determined that eggs stored at refrigerator temperature during the research, depending on the storage conditions, preserved their quality characteristics better than those stored at room temperature. The study concluded that the eggs of the ATAK-S genotype had a lower shelf life compared to those of the Lohmann Sandy and Lohmann Brown genotypes.

Post-Thaw Storage Temperature Influenced Boar Sperm Quality and Lifespan through Apoptosis and Lipid Peroxidation.

Cryopreservation deteriorates boar sperm quality and lifespan, which restricts the use of artificial insemination with frozen-thawed boar semen in field conditions. The objective of this study was to test the effects of post-thaw storage time and temperature on boar sperm survival. Semen ejaculates from five Landrace boars (one ejaculate per boar) were collected and frozen following a 0.5 mL-straw protocol. Straws from the five boars were thawed and diluted 1:1 (v:v) in BTS. The frozen-thawed semen samples were aliquoted into three parts and respectively stored at 5 °C, 17 °C, and 37 °C for up to 6 h. At 0.5, 2, and 6 h of storage, sperm motility, viability, mitochondrial membrane potential, and intracellular reactive oxygen species (ROS) levels and apoptotic changes were measured. Antioxidant and oxidant levels were tested in boar sperm (SPZ) and their surrounding environment (SN) at each timepoint. The results showed significant effects of post-thaw storage time and temperature and an impact on boar sperm quality (total and progressive motility, VCL, viability, acrosome integrity), early and late sperm apoptotic changes, and changes in MDA levels in SPZ and SN. Compared to storage at 5 °C and 37 °C, frozen-thawed semen samples stored at 17 °C displayed better sperm quality, less apoptotic levels, and lower levels of SPZ MDA and SN MDA. Notably, post-thaw storage at 17 °C extended boar sperm lifespan up to 6 h without obvious reduction in sperm quality. In conclusion, storage of frozen-thawed boar semen at 17 °C preserves sperm quality for up to 6 h, which facilitates the use of cryopreserved boar semen for field artificial insemination.

Agricultural wastes: a new promising source for phenylalanine ammonia-lyase as anticancer agent.

The present study aims to investigate the physicochemical characteristics of phenylalanine ammonia-lyase (PAL) extracted from agricultural waste and its potential use as an anticancer agent in comparison to microbial PAL. We extracted and partially purified PAL from agricultural waste sources. We assessed the temperature and pH range of PAL and determined enzyme kinetics parameters including Michaelis constants (Km), maximum velocity (Vmax), and specificity constant values (Vmax/Km). Additionally, we examined the effects of different storage temperatures on PAL activity. In our analysis, we compared the efficacy of agricultural waste-derived PAL with PAL from Rhodotorula glutinis. The results demonstrated that PAL extracted from agricultural waste exhibited significantly higher specific activity (Vmax/Km) compared to its microbial counterpart. The agricultural waste-derived PAL displayed a stronger affinity for phenylalanine, as indicated by a lower Km value than the microbial PAL did. Furthermore, PAL from agricultural waste maintained activity across a broader temperature and pH range (15-75°C, pH 5-11), in contrast to microbial PAL (20-60°C, pH 5.5-10). Importantly, the PAL derived from agricultural waste exhibited superior stability, retaining over 90% of its activity after 6months of storage at room temperature (25°C), whereas microbial PAL lost more than 70% of its activity under similar storage conditions. In anticancer experiments against various cancer cell lines, agricultural waste-derived PAL demonstrated greater anticancer activity compared to microbial PAL. These findings suggest that PAL sourced from agricultural waste has the potential to be a safe and effective natural anticancer agent.

Pre-analytical properties of different respiratory viruses for PCR-based detection: Comparative analysis of sampling devices and sample stabilization solutions

After the decline of the COVID-19 pandemic, health systems were challenged by the simultaneous prevalence of different respiratory viruses causing a wide overlap in symptoms. This increased the demand for multi-virus diagnostic tests which require suitable pre-analytical workflow solutions in order to receive valid diagnostic results. In this context, the effects of specimen storage duration and temperature on the RNA/DNA copy number stability of influenza A/B, RSV A/B, SARS-CoV-2 and adenovirus were examined for four commercially available transport swab systems and saliva collection devices. The respiratory viruses were more stable in the saliva collection devices than in the transport swab systems when stored at RT or 37 °C for up to 96 h. Moreover, no differences between viral nucleic acid stability of enveloped and non-enveloped viruses were observed. The infectivity of all enveloped viruses could be inactivated by the saliva collection device from PreAnalytiX. The Norgen saliva device completely inactivated influenza A/B, while RSV A/B were partially inactivated. The non-enveloped adenovirus was inactivated by a reduction factor of 10E+ 4 in both saliva collection devices. All respiratory viruses remained infectious in the transport swab systems. Two possible transport medium additives were tested which inactivated or strongly reduced viral replication of tested enveloped viruses but had no effect on the non-enveloped adenovirus. Finally the implementation of multi-target detection procedures involving a direct amplification approach was successfully tested by spike-in of all enveloped viruses simultaneously into transport swab systems. This fast and reproducible setup presents a valuable solution for future implementations in multi-virus testing strategies.

Effects of storage temperature and time on metabolite profiles measured in dried blood spots, dried blood microsamplers, and plasma

The practical advantages of capillary whole blood collection over venipuncture plasma collection for human exposome research are well known. However, before epidemiologists, clinicians, and public health researchers employ these microvolume sample collections, a rigorous evaluation of pre-analytical storage conditions is needed to develop protocols that maximize sample stability and reliability over time. Therefore, we performed a controlled experiment of dried whole blood collected on 10 μL Mitra microsamplers (DBM), 5-mm punches of whole blood from a dried blood spot (DBS), and 10 μL of plasma, and evaluated the effects of storage conditions at 4 °C, −20 °C, or −80 °C for up to 6 months on the resulting metabolite profiles measured with untargeted liquid chromatography-high resolution mass spectrometry (LC-HRMS). At −80 °C storage conditions, metabolite profiles from DBS, DBM, and plasma showed similar stability. While DBS and DBM metabolite profiles remained similarly stable at −20 °C storage, plasma profiles showed decreased stability at −20 °C compared to −80 °C storage. At refrigerated temperatures (4 °C), metabolite profiles collected on DBM were more stable than plasma or DBS, particularly for lipid classes. These results inform robust capillary blood sample storage protocols for DBM and DBS at potentially warmer temperatures than −80 °C, which may facilitate blood collections for populations outside of a clinical setting.

Retrogradation-induced physicochemical changes in pre-cooked rice noodles stored at different temperatures: a viewpoint from water dynamics and structure.

There has been significant interest in pre-cooked noodles that have a long shelf life and are convenient to cook. However, the thermal processes during preparation, and their high moisture content, can lead to significant quality deterioration during storage. Nevertheless, a comprehensive evaluation of these quality losses has not yet been conducted. The effects of different storage temperatures (25, 4, and -20 °C) on the retrogradation-related physicochemical changes in pre-cooked rice noodles were elucidated mainly from the water dynamics and structural viewpoints. Thermal analysis demonstrated that amylopectin recrystallization took place in the noodles stored at refrigerated temperature, followed by room temperature. The refrigerated storage accelerated the starch retrogradation that caused the water molecules to become entrapped within the crystalline structure by lowering the water hydration properties and weighted T2 relaxation times of the pre-cooked noodles. These water mobility patterns were correlated with the textural changes in the noodles (greater hardness and Rmax /extensibility). Furthermore, the higher structural density and thickness derived from starch retrogradation were observed in the tomographic and microscopic images of the refrigerated noodles. The principal component analysis demonstrated that various physicochemical changes of the pre-cooked noodles during storage showed high correlations with the degree of starch retrogradation (r > 0.83). The physicochemical features of the precooked noodles stored under refrigerated conditions were involved in the molecular dynamics of water, showing a notable water mobility reduction derived from the starch retrogradation, which contributed to their thermal, tomographical, and textural changes. © 2023 Society of Chemical Industry.

EFFORTS TO MAINTAIN QUALITY AND EXTEND THE SHELF LIFE OF TANGERINE CITRUS FRUITS WITH PACKAGING AND STORAGE TEMPERATURE

Citrus fruit is a non-climacteric fruit, this fruit must be picked when it is ripe optimally. Post-harvest methods include harvest collection, washing and cleaning, quality classification, packaging and storage. Apart from being expected to have a good shelf life, citrus fruit must also have hight quality and be able to maintain its nutritional content for consumers' needs. The aim of this research was to determine the effect of storage and packaging temperatures on changes in the quality and shelf life of Tangerine Citrus. The research was conducted in October to November 2021 at the Laboratory of Agrotechnology, Trilogi University. This research method uses a randomized block design consisting of two factors and three replications. The first factor is that packaging consists of two levels, namely packaging using wrapping and non-wrapping. The second factor is temperature which consists of two levels: 15°C and 25°C. The result of this study is that citrus with a non-wrapping treatment with a storage temperature of 15 oC is the best treatment and can be consumed up to 8 days after treatment, this can be seen from some of the lowest weight shrinkage percentage, scoring appearance, color, and taste that is still acceptable to consumers compared to other treatments.

One-plasmid double-expression system for preparation of MS2 virus-like particles packaging SARS-CoV-2 RNA.

COVID-19 is a disease caused by a virus named SARS-CoV-2. SARS-CoV-2 is a single-stranded positive-sense RNA virus. Reverse transcription quantitative PCR (RT-qPCR) assays are the gold standard molecular test for detection of RNA viruses. The aim of this study was to construct an RNA-positive control based on MS2 phage-like particles (MS2 VLPs) to detect SARS-CoV-2 RNA. pCDFDuet-1 was used as a one-plasmid double-expression system to construct MS2 VLPs containing ssRNA of SARS-CoV-2. The sequence encoding one copy of maturase, His-tag and coat protein dimer was cloned and inserted into MCS1 of the plasmid; the fragment encoding protein N and ORF1ab from SARS-CoV-2 was cloned and inserted into MCS2. The prepared plasmid was transformed into Escherichia coli strain BL2 (DE3), and expression of the construct was induced by 1 mM isopropyl-L-thio-D-galactopyranoside (IPTG) at 30°C for 12 hours. MS2 VLPs were purified and collected with Ni-NTA affinity chromatography columns. The size and shape of the MS2 VLPs were verified by transmission electron microscopy, and the stability of MS2 VLP packaged RNA was evaluated by treatment with RNase A. Effects of storage temperature and buffer on MS2 VLP stability were also investigated. The results showed that SARS-CoV-2 MS2 VLPs could be successfully produced by this one-plasmid double-expression system. MS2 VLPs showed high stability and may be used as a positive control in molecular diagnosis of COVID-19.