Effect Of Selenium Supplementation Research Articles

The effect of selenium supplementation in organic and in organic form on oxidant balance in rat heart and femoral muscle

Total antioxidant status (TAS), activity of antioxidant enzymes - superoxide dismutase (SOD) and glutathione peroxidase (GPx), concentrations of non-enzymatic antioxidants - ascorbic acid (AA) and reduced glutathione (GSH) as well as concentration of lipid peroxidation marker - malonyldialdehyde (MDA) were determined in heart and femoral muscle of rats receiving different selecompounds (inorganic selenite and organic selenosemicarbazide of chain structure and selenazoline of ring structure). Chain selenosemicarbazide markedly decreased TAS values vs. control without Se-supplementation in heart. GPx was significantly depressed vs. control in heart of animals receiving organic selenium. Ring selenazoline decreased heart AA, whereas selenosemicarbazide increased heart GSH. Inorganic selenite diminished femoral muscle GSH. Selenium supplementation distinctly inhibited process of lipid peroxidation - MDA was decreased in Se-given animals, particularly in heart. As organic ring selenocompound depressed heart MDA to the highest degree, did not impair total antioxidant status and caused no disturbance of antioxidant barrier in femoral muscle, it could be suggested that further research may reveal possibilities of its application as a Se-supplement.

Reduced Selenium-Binding Protein 1 in Breast Cancer Correlates with Poor Survival and Resistance to the Anti-Proliferative Effects of Selenium

Supplemental dietary selenium is associated with reduced incidence of many cancers. The antitumor function of selenium is thought to be mediated through selenium-binding protein 1 (SELENBP1). However, the significance of SELENBP1 expression in breast cancer is still largely unknown. A total of 95 normal and tumor tissues assay and 12 breast cancer cell lines were used in this study. We found that SELENBP1 expression in breast cancer tissues is reduced compared to normal control. Low SELENBP1 expression in ER+ breast cancer patients was significantly associated with poor survival (p<0.01), and SELENBP1 levels progressively decreased with advancing clinical stages of breast cancer. 17-β estradiol (E2) treatment of high SELENBP1-expressing ER+ cell lines led to a down-regulation of SELENBP1, a result that did not occur in ER– cell lines. However, after ectopic expression of ER in an originally ER– cell line, down-regulation of SELENBP1 upon E2 treatment was observed. In addition, selenium treatment resulted in reduced cell proliferation in endogenous SELENBP1 high cells; however, after knocking-down SELENBP1, we observed no significant reduction in cell proliferation. Similarly, selenium has no effect on inhibition of cell proliferation in low endogenous SELENBP1 cells, but the inhibitory effect is regained following ectopic SELENBP1 expression. Furthermore, E2 treatment of an ER silenced high endogenous SELENBP1 expressing cell line showed no abolishment of cell proliferation inhibition upon selenium treatment. These data indicate that SELENBP1 expression is regulated via estrogen and that the cell proliferation inhibition effect of selenium treatment is dependent on the high level of SELENBP1 expression. Therefore, the expression level of SELENBP1 could be an important marker for predicting survival and effectiveness of selenium supplementation in breast cancer. This is the first study to reveal the importance of monitoring SELENBP1 expression as a potential biomarker in contributing to breast cancer prevention and treatment.

Negative association of smoking with PSA and PSA velocity.

e16060 Background: Smoking and obesity have been implicated in the pathogenesis of various chronic diseases and carcinogenic processes. However, their role in prostate cancer (PCa) &amp; their association with important biomarkers such as prostate specific antigen (PSA) &amp; PSA velocity (rate of PSA change over time) remains unresolved. This study investigates the association of these risk factors with PSA &amp; PSA velocity (PSAV) in men at high risk for PCa. Methods: Data were obtained from 699 men participating in a Phase 3 clinical trial aimed at investigating the effect of selenium supplementation on incidence of PCa. During the course of this trial 73 men were diagnosed with PCa. Multiple linear regressions were used to investigate the association with PSA and mixed effects models were used to investigate the association with PSAV. Results: As compared to non-smokers, smokers demonstrated statistically significant negative association with PSA (8.0 &amp; 6.7ng/ml, p=0.02). This association was more pronounced in men diagnosed with PCa (14.7 &amp; 9.9ng/ml) as compared to those not diagnosed with PCa (4.5 &amp; 3.6ng/ml). As compared to non-obese subjects, obese subjects did not demonstrate a statistically significant association with PSA (6.8 &amp; 7.1ng/ml, p= 0.64) nor do these results change after stratifying the analysis by PCa status. Smoking was associated with statistically significant lower PSAV (p=0.008) whereas obesity was not (p=0.42). Using the standard PSA level of 4ng/ml as a cut off for conducting prostate biopsy, 14.6% of smokers in this study would not have been prescribed a prostate biopsy and thus misclassified as being negative for PCa. Conclusions: The negative association of smoking with PSA and PSA velocity could potentially be due to its negative effect on testosterone. Hence, increased vigilance may be warranted among smokers with borderline PSA to prevent the risk of delayed diagnosis and metastasis thus reducing morbidity and mortality associated with PCa.

The effect of selenium supplementation on vaccination response and immune function in adult horses1

Selenium status has been reported to affect immune function across many different species. Yet few studies have focused on the effect of Se status on the equine immune system. This study examined the effect of Se supplementation on vaccination response and immune function in mature horses. Twenty-eight horses were blocked by age and sex and were randomly allocated to 1 of 4 dietary treatment groups: low Se (LS), adequate Se (AS), Se-yeast (SP), and sodium selenite (SS). For 35 wk, horses allocated to LS, SP, and SS received a low-Se diet (0.06 mg/kg DM) with the intention to lower Se stores, whereas AS received an adequate Se diet (0.12 mg/kg DM). A 29-wk repletion phase was as follows: LS and AS were kept on the diets fed during the depletion period, whereas SP and SS received the depletion diet plus their respective Se supplements to achieve a dietary Se concentration of 0.3 mg/kg DM. The Se status of the horses was monitored using whole blood Se and glutathione peroxidase (GSH-Px) activity as indicators. At wk 22 and 25 of the repletion phase, horses were vaccinated intramuscularly with 10 mg ovalbumin (OVA). Horses were also vaccinated against equine influenza at wk 25. Blood samples were collected for 7 wk after initial vaccination for serum separation and at 0, 3, and 5 wk postvaccination for peripheral blood mononuclear cell (PBMC) isolation and whole blood cytokine mRNA evaluation. At wk 22 of the repletion phase, both Se and GSH-Px were greater for SP and SS compared with AS and LS (P < 0.001). Serum vitamin E was similar between treatments. Response to OVA vaccination, evaluated as OVA-specific IgG production, cytokine mRNA expression of PBMC stimulated with OVA in vitro, and lymphocyte proliferation, was unaffected by Se status. Similarly, memory response to the influenza vaccine was not affected by Se status. However, decreased mRNA expression of selected cytokines was observed in PBMC stimulated with phorbol 12-myristate 13-acetate for LS compared with AS, SP, and SS (P < 0.05). Whole blood mRNA expression of IL-10 was greater for SS compared with LS, AS, and SP (P = 0.043). Although the OVA and influenza vaccination responses were unaffected by Se status, other measures of immune function did indicate that low Se status could adversely affect cell-mediated immunity.

Abstract 3615: Does selenium supplementation increase serum glucose levels in men at high risk for prostate cancer.

Abstract Background: Current literature regarding the effect of selenium supplementation on risk of diabetes is inconclusive. Hence a longitudinal study was conducted to investigate the effect of selenium supplementation on serum glucose levels in elderly men. Methods: Data were obtained from 699 men participating in a randomized, double-blind, placebo-controlled Phase 3 clinical trial investigating the effect of two doses of selenium (200 and 400 μg/day) compared to placebo on incidence of prostate cancer. Subjects were followed every six months for up to five years. Serum glucose levels were obtained every six months. Mixed effects regression models were used to assess if rate of change of serum glucose levels was significantly different in the selenium supplemented groups as compared to placebo. Sensitivity analyses were performed to assess the robustness of findings and to minimize the possibility of residual bias due to fasting status. Results: Of the total 2893 glucose measurements, 734 were carried out when the subject had been fasting for ≥ 8 hours. The changes in serum glucose levels during the course of the trial were not statistically significantly different as compared to placebo for the selenium 200 μg/day (p = 0.88) or selenium 400 μg/day (p = 0.45) treatment groups. Sensitivity analyses demonstrated comparable results for models using the total population and models restricted to subjects with only fasting glucose data. Conclusion: These results do not support a relationship between selenium supplementation and risk of diabetes. Hence recommendations regarding selenium supplementation based on increased risk of diabetes seem premature. Citation Format: Amit M. Algotar, C H. Hsu, Steven P. Stratton. Does selenium supplementation increase serum glucose levels in men at high risk for prostate cancer. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 3615. doi:10.1158/1538-7445.AM2013-3615

Effect of Selenium Supplementation on Thyroid Function in UK Pregnant Women: a Randomised, Controlled Pilot Trial

Effect of Selenium Supplementation on Thyroid Function in UK Pregnant Women: a Randomised, Controlled Pilot Trial

Effect of selenium supplement on proteome of chicken liver

The aims of the present study were to optimize a two dimensional polyacrylamide gel electrophoresis method to chicken liver proteome and to determine the changes of protein expression caused by selenium. Twelve broiler chicken were used in this experiment. The selenium intake was 0,2 mg/kg in the control group (6 chicken) and 4,25 mg/kg in the experimental group (6 chicken). Using the optimized proteomic approach, we have succesfully separated 747 proteins in the experimental group and 741 proteins in the control group. We found six proteins, wich expressed only in the samples of experimental group. Further investigations need to determine these six proteins with mass spectrometry (MS) and look for the correlation between the physiological effects of selenium and the expression of these proteins.

Design and Baseline Characteristics of Participants in a Phase III Randomized Trial of Celecoxib and Selenium for Colorectal Adenoma Prevention

COX inhibitors reduce colorectal adenoma recurrence by up to 45% and selenium supplementation may prevent colorectal cancer. Following colonoscopic adenoma resection, 1,600 men and women, ages 40 to 80 years, were randomized to celecoxib (400 mg daily), a selective COX-2 inhibitor, and/or selenium (200 μg daily as selenized yeast), or double placebo. The trial was initiated in November 2001. The primary trial endpoint is adenoma recurrence in each intervention group compared with placebo, as determined by surveillance colonoscopy conducted three to five years after baseline. Randomization was stratified by use of low-dose aspirin (81 mg) and clinic site. Following reports of cardiovascular toxicity associated with COX-2 inhibitors, the celecoxib arm was discontinued in December 2004 when 824 participants had been randomized. Accrual continued with randomization to selenium alone or placebo. Randomization of the originally planned cohort (n = 1,621) was completed in November 2008. A further 200 patients with one or more advanced adenomas (denoting increased risk for colorectal cancer) were accrued to enhance statistical power for determining intervention efficacy in this higher-risk subgroup. Accrual of the total cohort (n = 1,824) was completed in January 2011. Baseline cohort characteristics include: mean age 62.9 years; 65% male; body mass index (BMI) 29.1 ± 5.1; 47% taking low-dose aspirin while on trial; 20% with three or more adenomas; and 38% with advanced adenomas. Intervention effects on adenoma recurrence will be determined, and their modification by genetic background and baseline selenium level. The effect of selenium supplementation on risk for type II diabetes will also be reported.

Effects of Selenium Supplementation on Expression of Endothelin-1 and Its Receptors in Pulmonary Microvascular Endothelial Cells from Chick Embryos

The objective of this study was to evaluate the effects of supplemental selenium (Se) on expression of endothelin-1 (ET-1) and its receptors in cultured chick embryos pulmonary microvascular endothelial cells (PMVECs). To accomplish this, PMVECs were treated in Se-deficient or Se-supplement (12, 24, 50, 100ng/ml) culture medium for 48h. Low Se medium was achieved by reducing serum concentrations and the essential growth factors were added. After the incubation, the effects of supplemental Se on ET-1 and its receptors gene expression were assessed by quantitative real-time PCR (qRT-PCR). Compared with the control group, our results showed that among the different concentrations of Se supplement, the levels of ET-1 gene expression treated with both the moderate Se doses (24, 50ng/ml, P < 0.01, P < 0.01, respectively) and the high doses (100ng/ml, P < 0.05) were noticeably decreased, the low-dose group (12ng/ml), which showed no changes. Meanwhile, Se supplement (24, 50, 100ng/ml) was found to be effective in reducing the expression levels of ETA (P < 0.01, P < 0.05, P < 0.05, respectively) in cultured PMVECs grown in low Se medium. However, there were no significant changes (P > 0.05) in ETB mRNA levels during the cell proliferation. These observations indicated that Se may play both direct and indirect role in the regulation of ET-1 and its receptors gene expression and their production in avian PMVECs. Se supplement decreases in ET-1 and ETA production in Se-deficient PMVECs may partly explain the mechanism of the protective effects of the Se on the cardiovascular system.

Organic Selenium Supplementation Increases Mercury Excretion and Decreases Oxidative Damage in Long-Term Mercury-Exposed Residents from Wanshan, China

Due to a long history of extensive mercury mining and smelting activities, local residents in Wanshan, China, are suffering from elevated mercury exposure. The objective of the present study was to study the effects of oral supplementation with selenium-enriched yeast in these long-term mercury-exposed populations. One hundred and three volunteers from Wanshan area were recruited and 53 of them were supplemented with 100 μg of organic selenium daily as selenium-enriched yeast while 50 of them were supplemented with the nonselenium-enriched yeast for 3 months. The effects of selenium supplementation on urinary mercury, selenium, and oxidative stress-related biomarkers including malondialdehyde and 8-hydroxy-2-deoxyguanosine were assessed. This 3-month selenium supplementation trial indicated that organic selenium supplementation could increase mercury excretion and decrease urinary malondialdehyde and 8-hydroxy-2-deoxyguanosine levels in local residents.

Effect of selenium supplementation on the structure of the lung of adult albino rats subjected to experimentally induced chronic renal failure

Effect of selenium supplementation on the structure of the lung of adult albino rats subjected to experimentally induced chronic renal failure

Effects of selenium supplementation and chronic inflammation on bone microarchitecture and strength in mice

Timed‐pregnant C57BL6 mice were fed a Torula yeast selenium‐depletion diet from the final days of gestation through lactation. At 23 days of age, pups were weaned and randomly assigned to the depletion diet or to diets supplemented with 0.2, 2 or 4 mg/kg diet of Se added as sodium selenate for 14 weeks. At 96–98 days of age mice were randomly assigned to placebo or to lipopolysaccharide (E. coli Serotype 0127:B8) treatment to produce chronic inflammation. Time release pellets (0 or 0.1 μg/g body weight/d) were implanted subcutaneously. Body weight was reduced by LPS 14 days after implantation but differences were no longer significant by 28 days. Bone microarchitecture was evaluated in tibia and 4th lumbar vertebra (L4) and force to compress was estimated on trabecular cores using finite element analysis (Scanco μCT40). Bone volume/total volume, connectivity density, and trabecular number were significantly reduced by LPS treatment while trabecular separation was increased by LPS treatment in both bones. In spine significant interactions affected size‐independent stiffness and von Mises stresses. Overall, chronic inflammation at a level that does not produce severe body weight loss still significantly impaired measures of bone microarchitecture and strength. (Supported by Oklahoma Center for Advancement of Science and Technology).

Effects of selenium supplementation on serum cortisol in repeatedly handled cattle

This study evaluated the relationship between Se supplementation and serum cortisol in repeatedly handled cattle. Sixty Nellore calves were randomly distributed into four experimental groups, which were fed a mineral-protein mixture added with Se to achieve individual daily supplementation of 0 (Gc), 3.6 (G 3.6 ), 5.4 (G 5.4 ) and 6.4 (G 6.4 ) mg Se. Stressful handling procedures and blood sampling were performed on days 0, 15, 30, 60, 90 and 120. Serum cortisol increased until day 90 and decreased on day 120, irrespective of Se supplementation. This finding shows that cattle reached a stress state but adapted. Cortisol and Se levels were not markedly correlated. Serum Se increased over the course of the experiment in the supplemented groups. However, because serum Se decreased in Gc, it may have mobilized to form the selenoproteins needed to remove metabolic wastes from handling-related oxidative stress.

Selenium Supplementation Can Slow the Development of Naphthalene Cataract

Purpose: To evaluate the effect of selenium supplementation on the progress of naphthalene cataract.Materials and Methods: Sprague-Dawley rats were randomly divided into five groups as follows: normal control, naphthalene control and selenium-supplemented groups (Selenium I, II and III, which were orally administrated with selenium at doses of 0.0104 mg/kg, 0.0208 mg/kg and 0.0416 mg/kg, respectively). All the intervention groups were orally administered with 10% naphthalene solution for 5 weeks. The lens density of each group was determined by photography. Moreover, glutathione peroxidase (GPx) activity in the lens, erythrocyte and plasma was investigated. In addition, lens glutathione (GSH), malondialdehyde (MDA) and hydroxyl radical levels were evaluated. Selenium level in aqueous humor was determined using atomic absorption spectrometry.Results: The maximum, mean and minimum densities of lens opacities were lower in Selenium group II and III than those in naphthalene group. The maximum density of the lens increased more slowly in Selenium group I than that in naphthalene controls. In selenium-supplemented groups, blood and lens GPx activities as well as aqueous humor selenium level increased significantly. Selenium supplementation also significantly ameliorated the decrease in GSH level and increase in MDA and hydroxyl radical levels in the lens of naphthalene-treated rats.Conclusions: Selenium supplementation could slow the development of naphthalene cataract possibly by attenuating the oxidative stress in the lens.

Effects of selenium supplementation in the early stage of autoimmune thyroiditis in childhood: an open-label pilot study

The aim of the study was to evaluate the role of selenium (Se) in childhood autoimmune thyroiditis regarding its effect on thyroid-stimulating hormone (TSH), free thyroxine (fT4), thyroid peroxidase antibodies (TPOAb), thyroglobulin antibody (TgAb), and thyroid morphology. Newly diagnosed 23 euthyroid children (mean age, 12.3 +/- 2.4 years) with Hashimoto thyroiditis (HT) received only 50 microg L-selenomethionine per day for 3 months. The baseline basal urinary iodine level, serum Se, TSH, fT4, TPOAb, and TgAb concentrations, and thyroid morphology by ultrasound were detected. We reanalyzed the TPOAb and TgAb changes at the 3rd month and then compared the thyroid morphology with 30 healthy individuals (mean age, 12.1 +/- 2.1 years) at the 6th month. Serum TPOAb, TgAb, and thyroid echogenicity were unchanged with Se supplementation. A prominent decrease in thyroid volume was noteworthy; 35% of patients showed a thyroid volume regression rate of > or = 30%. In terms of TPOAb and TgAb, Se may not benefit in the euthyroid period of HT, but Se supplementation seems to lead a favorable response in thyroid volume regression.

The effect of selenium supplementation on elements distribution in liver of rats subject to strenuous swimming

The present study aims to explore how selenium supplementation affects the element distribution in the liver tissue of rats subjected to strenuous swimming exercise. Thirty-two Spraque-Dawley male rats were equally divided into the four groups: Group 1, normal control group. Group 2, selenium-supplemented, non-swimming (0.6 mg/kg/day sodium selenite) group. Group 3, swimming, no supplementation group. Group 4, swimming, selenium-supplemented (0.6 mg/kg/day sodium selenite) group. After one month, the animals were decapitated and liver tissue samples were collected to determine the levels of lead, cobalt, boron, molybdenum, chromium, sulfur, magnesium, sodium, potassium, phosphorus, copper, iron, zinc and selenium. The chromium, molybdenum, iron, sodium and potassium values were higher in the swimming groups, relative to controls. Group 3 had significantly lower lead levels (p<0.001). The highest cobalt levels were obtained in the Group 1 and that of the Group 2 was higher than in the Groups 3 and 4. The boron values in the Group 3 were higher than those in all other groups. The copper and magnesium levels were higher in the Groups 3 and 4, compared to the Groups 1 and 2. The highest phosphorus levels were found in the Group 1. The highest selenium and zinc values were obtained in the Group 2 and those of the Group 4 were higher than in the Groups 1 and 3. Group 1 had higher selenium and zinc levels than the Group 3. The results of the present study demonstrated that selenium-supplemented rats subjected to strenuous swimming exercise had distinct elements distribution in liver tissue. Also, selenium supplementation offsets the decrease in zinc levels in rats subjected to vigorous swimming (Tab. 3, Ref. 20).

Effect of Selenium Supplementation on Glutathione Peroxidase and Catalase Activities in Senescent Cultured Human Fibroblasts

Aims: To investigate the effect of senescence and selenium supplementation on glutathione peroxidase (cGPx) and catalase (CAT) activities, and concurrent hydrogen peroxide (H₂O₂) generation in subcultured human fibroblasts. Methods: cGPx and CAT activities and H₂O₂ levels were assayed in presenescent passage 5 and 10 cells, and in senescent passage 20, 25, 30 and 35 cells cultured in routine medium (MEM1) and supplemented media MEM2 and MEM3 containing normal and triple human plasma levels of Se, respectively. Senescent cells were identified by studying their growth and replication states, and by monitoring their activity of key glucose and glycogen degradative enzymes. Results: cGPx activity showed moderate increases in senescent cells at passages 20–35 subcultured in MEM1 or MEM2. This activity underwent highly significant progressive increases in the same senescent cells subcultured in MEM3. In contrast, CAT activity showed progressive, highly significant increases in senescent cells at passages 20–35 regardless of the culture medium type. Concurrent H₂O₂ generation was significantly increased in passage 15–25 cells and peaked to higher levels in passage 30 and 35 cells cultured in MEM1 or MEM2. These rates, however, were significantly reduced in senescent passage 20–35 cells cultured in MEM3. Conclusions: The highest cGPx activity and coupled lower H₂O₂ generation were achieved in senescent cells cultured in MEM3.

Effects of Selenium Supplements on Cancer Prevention: Meta-analysis of Randomized Controlled Trials

This meta-analysis aimed to investigate the preventive effect of selenium supplements alone on cancer as reported by randomized controlled trials (RCTs). We searched PubMed, EMBASE, and the Cochrane Library in July 2009. Of the 461 articles searched, 8 articles on 9 RCTs, which included 152,538 total participants, 32,110 in antioxidant supplement groups, and 120,428 in placebo groups, were included. In a random-effects meta-analysis of all 9 RCTs, selenium supplementation alone was found to have an overall preventive effect on cancer incidence [relative risk (RR) = 0.76; 95% confidence interval (CI) = 0.58–0.99]. Among subgroup meta-analyses, the preventive effect of selenium supplementation alone on cancer was apparently observed in populations with a low baseline serum selenium level (<125.6 ng/mL) (RR = 0.64; 95% CI = 0.53 to 0.78; I2 = 45.5%; n = 7) and in high-risk populations for cancer (RR = 0.68; 95% CI = 0.58 to 0.80; I2 = 41.5%; n = 8). The meta-analysis of randomized controlled trials indicates that there is possible evidence to support the use of selenium supplements alone for cancer prevention in the low baseline serum selenium level population and in the high-risk population for cancer.

Immunomodulatory effect of dietary selenium supplementation on serum antibody response and leucocytic profile of Trypanosoma brucei brucei infected rats

Effect of selenium supplementation on the immune response of Trypanosomabrucei brucei infected rats was investigated. Twenty five (25) adult male albino rats divided into five groups of five rats each were used for this study. Groups A, B and C were fed 4, 8 and 16 part per million (ppm) selenium in their feed, respectively. Groups D and E were not given selenium supplementation. The supplementation started on day 0, followed by the infection of groups A, B, C and D with T. brucei brucei on day 14 post supplementation (PS). Immune response of the rats was assessed by determining the antibody response to sheep red blood cells (SRBC) using direct haemagglutination technique and total and differential leucocyte counts. The supplementation led to significant (p < 0.05) increase in antibody response to sheep red blood cell of the supplemented groups at pre- and post infection when compared with the control. The infection however, led to decrease in antibody titre but remained higher than the pre-supplementation titre. Also, the supplementation led to increase (p < 0.05) in leucocyte counts prior to infection on day 14 PS. The increase in total leucocyte count could be attributed to increase in lymphocyte and neutrophils. The mortality record showed that all rats (100%) in the infected, not supplemented group and 2 rats (40%) died from the 16 ppm group by day 42 PS. No rat died in 4, 8 ppm and not infected, not treated groups. Key words: Selenium, antibody titre, leucocytes, trypanosomes, immunosuppression.

Effects of selenium supplementation on the muscle contractile properties, fatigue and antioxidant enzymes activities of gastrocnemius muscles in trained and untrained rats

Selenium (Se) is an important component of cellular seleno ‐compounds andan integral component of glutathione Peroxidase (GPx), which catalyzes thereduction of harmful radicals produced during muscular exercise. The currentstudy was carried out to evaluate the muscle contractile properties andfatigue resistance of gastrocnemius muscle under selenium supplementationschemes in sedentary and exercise protocols as well as measure the antioxidantenzymes activity and lipid peroxidation. Rats were divided into fourgroups; sedentary Selenium supplementation (S), exercise Selenium (SE)groups, sedentary control (SC) and exercise control (EC) groups. The ratswere fed with 80 μg/kg body weights selenium for six weeks. The exerciseprotocol consisted the 40 jumps up to the height of 40 cm for 6 ‐week. Themuscle fatigue protocol consisted the trains of pulses of 40 Hz at every secondfor at least 2 min. Significant (P&lt;0.05) increase was observed in treatedgroups than control in the muscle contractile properties like twitch tension(Pt), contraction time (CT) and twitch/tetanic tension Ratio (Pt/Po Ratio),tetanic tension (Po) and EMG amplitude. Decreased EMG failure and increasedfatigue index were observed in ES group. Moreover, a significant(P&lt;0.05) increase and decrease in the GPx activity and lipid peroxidation respectivelywas also reported than SC and EC group. While there were nochanges reported in the activity of CAT and SOD enzymes. This study revealedthat the Se with jumping exercise induces muscle contractile propertiesand decreases the muscular fatigue.