Prosocial Effects Research Articles

Mephedrone induces partial release at human dopamine transporters but full release at human serotonin transporters

Mephedrone (4-methylmethcathinone) is a cathinone derivative that is recreationally consumed for its energizing and empathogenic effects. The stimulating properties are believed to arise from the ability of mephedrone to interact with the high-affinity transporters for dopamine (DA) (DAT) and norepinephrine (NET), whereas the entactogenic effect presumably relies on its activity at the serotonin (5-HT) transporter (SERT). Early studies found that mephedrone acts as a releaser at NET, DAT and SERT, and thus promotes efflux of the respective monoamines. Evidence linked drug-induced reverse transport of 5-HT via SERT to prosocial effects, whereas activity at DAT is strongly correlated with abuse liability. Consequently, we sought to evaluate the pharmacology of mephedrone at human (h) DAT and SERT, heterologously expressed in human embryonic kidney 293 cells, in further detail. In line with previous studies, we report that mephedrone evokes carrier-mediated release via hDAT and hSERT. We found this effect to be sensitive to the protein kinase C inhibitor GF109203X. Electrophysiological recordings revealed that mephedrone is actively transported by hDAT and hSERT. However, mephedrone acts as a full substrate of hSERT but as a partial substrate of hDAT. Furthermore, when compared to fully efficacious releasing agents at hDAT and hSERT (i.e. S(+)-amphetamine and para-chloroamphetamine, respectively) mephedrone displays greater efficacy as a releaser at hSERT than at hDAT. In summary, this study provides additional insights into the molecular mechanism of action of mephedrone at hDAT and hSERT.

Are we braver in cyberspace? Social media anonymity enhances moral courage

The literature has established accumulated evidence on the negative consequences of social media anonymity on behaviors online (e.g., cyber-aggression). Yet the potential benefits of social media anonymity have been largely overlooked, especially when it comes to prosociality. In four studies, we examined the facilitating effect of perceived social media anonymity on online moral courage. We first tested and confirmed the relation of perceived social media anonymity to online moral courage in a correlational study (Study 1) and an experimental study (Study 2). We then tested and revealed the mediating role of perceived risk and the moderating role of moral meaningfulness in the relation between perceived anonymity and moral courage (Study 3). We further used social media behavioral data to examine the association between social media anonymity and moral courage in an ecologically valid context (Study 4). Our findings enrich the research of moral psychology and social media studies by providing the first experimental evidence for the prosocial effect of social media anonymity. They further have important implications for website interface design, social activism, as well as intervention programs to promote constructive civil engagement online.

Virtual social interactions during the COVID-19 pandemic: the effect of interpersonal motor synchrony on social interactions in the virtual space

Although the link between motor synchrony and emotional alignment has been extensively studied during face-to-face interaction, the question of whether this association also exists in virtual settings has remained unanswered. Here, we examined whether this link exists during virtual social interactions and whether pro-social effects will be induced during those interactions. To this end, two strangers shared difficulties they have experienced due to the COVID-19 pandemic during a virtual social interaction that included both audio and video. The findings revealed that motor synchrony and emotional alignment can arise spontaneously during a virtual social interaction between two strangers. Moreover, this interaction yielded a decrease in negative affect and an increase in positive affect, as well as an increase in feelings of trust, liking, cohesion, self-other overlap, and similarity between the strangers. Finally, a higher level of synchrony during the virtual interaction was specifically associated with increased positive emotional alignment and liking. It can thus be presumed that virtual social interactions may share similar characteristics and social effects as face-to-face interactions. Considering the tremendous changes the COVID-19 pandemic has caused regarding social communication, these findings may provide grounds for developing new intervention protocols aimed at dealing with the consequences of social distancing.

Cannabidiol and cannabis-inspired terpene blends have acute prosocial effects in the BTBR mouse model of autism spectrum disorder.

Cannabidiol (CBD) is a non-intoxicating phytocannabinoid with increasing popularity due to its purported therapeutic efficacy for numerous off-label conditions including anxiety and autism spectrum disorder (ASD). Those with ASD are commonly deficient in endogenous cannabinoid signaling and GABAergic tone. CBD has a complex pharmacodynamic profile that includes enhancing GABA and endocannabinoid signaling. Thus, there is mechanistic justification for investigating CBD's potential to improve social interaction and related symptoms in ASD. Recent clinical trials in children with ASD support CBD's beneficial effects in numerous comorbid symptoms, but its impact on social behavior is understudied. Here, we tested the prosocial and general anxiolytic efficacy of a commercially available CBD-rich broad spectrum hemp oil delivered by repeated puff vaporization and consumed via passive inhalation in the female cohort of the BTBR strain, a common inbred mouse line for preclinical assessment of ASD-like behaviors. We observed that CBD enhanced prosocial behaviors using the 3-Chamber Test with a different vapor dose-response relationship between prosocial behavior and anxiety-related behavior on the elevated plus maze. We also identified that inhalation of a vaporized terpene blend from the popular OG Kush cannabis strain increased prosocial behavior independently of CBD and acted together with CBD to promote a robust prosocial effect. We observed similar prosocial effects with two additional cannabis terpene blends from the Do-Si-Dos and Blue Dream strains, and further reveal that these prosocial benefits rely on the combination of multiple terpenes that comprise the blends. Our results illustrate the added benefit of cannabis terpene blends for CBD-based treatment of ASD.

Are they giving scarce resources away?: Types of prosocial behavior modulate the prosocial effects of target social class on others

People tend to perceive others' prosocial behavior as valuable and genuine when it is costly. Given that money (vs. time) is relatively scarcer for lower-class (vs. upper-class) people, we propose that the effect of target social class on others' prosocial intentions would vary across the donation and volunteering contexts. Specifically, we hypothesized that people would exhibit greater motivation to engage in prosocial behaviors when observing donation from lower-class targets than from those of upper-class, whereas the opposite would be the case for volunteering. The results across four studies (N = 984; Study 3 preregistered) supported our predictions. The predicted interaction effect between target social class and the type of prosocial behaviors emerged not only in participants' prosocial intentions (Studies 1–3), but also in their behavioral intentions (Study 4). Furthermore, the present research showed that perceived resource scarcity (Studies 2–4), self-reflection (Study 3), and genuineness (Study 4) served as significant mediators for the interaction effect between target social class and types of prosocial behaviors on participants' prosocial intentions.

Returning thanks to God and others: prosocial consequences of transcendent indebtedness

ABSTRACT Gratitude and indebtedness facilitate cooperative relationships and prosocial behavior. Yet, studies are needed to assess whether indebtedness and gratitude have differential prosocial effects. Study 1 (N = 659; highly religious emerging adult sample) employed a 3 × 2 experimental design with six conditions; 1) gratitude-only to humans, 2) indebtedness-only to humans, 3) gratitude and indebtedness to humans, 4) gratitude-only to God, 5) indebtedness-only to God, and 6) gratitude and indebtedness to God. Study 2 (N = 1,081; nationally representative sample) was a replication of Study 1. People prompted to experience both gratitude and indebtedness had higher prosocial relational engagement (i.e. consistent effects for relationship proximity and direct reciprocity) than those who primarily experienced gratitude or indebtedness. Transcendent indebtedness (want to repay) was related to better prosocial outcomes than transactional indebtedness (have to repay). Secure attachment to God moderated the association between receiving a favor from God (versus human) and increased prosocial behavior.

Perspective: Focused-Ultrasound Guided Neuropeptide Delivery as a Novel Therapeutic Approach in Psychiatry

Although drugs are a critical component of mental healthcare, most have modest benefits and significant side effects. One way to develop a superior intervention would be to administer drugs with the spatial and temporal precision that better replicates natural diversity within neurotransmitter systems. A technology called focused-ultrasound (FU) may be able to safely and transiently disrupt the blood-brain barrier with spatial precision, permitting the site-specific delivery of molecules that do not conventionally cross the blood-brain barrier. If this method is proven to be safe and effective in larger human trials, it may trigger a paradigm shift in biopsychology research where the level of precision with which neurotransmitter systems can be influenced is massively increased. In this article, we use the example of oxytocin in the treatment of Autism. We propose that intranasal administration is not highly effective because it leads to oxytocin’s wide dispersion throughout the brain, failing to specifically stimulate oxytocin’s prosocial effects in specific regions. Consequently, we hypothesize that site-specific delivery of oxytocin, particularly in brain regions such as the Nucleus Accumbens and Ventral Tegmental Area, would lead to more consistent benefits.

Deterrence through punishment can resolve collective risk dilemmas in carbon emission games.

Collective risk social dilemmas are at the heart of the most pressing global challenges we are facing today, including climate change mitigation and the overuse of natural resources. Previous research has framed this problem as a public goods game (PGG), where a dilemma arises between short-term interests and long-term sustainability. In the PGG, subjects are placed in groups and asked to choose between cooperation and defection, while keeping in mind their personal interests as well as the commons. Here, we explore how and to what extent the costly punishment of defectors is successful in enforcing cooperation by means of human experiments. We show that an apparent irrational underestimation of the risk of being punished plays an important role, and that for sufficiently high punishment fines, this vanishes and the threat of deterrence suffices to preserve the commons. Interestingly, however, we find that high fines not only avert freeriders, but they also demotivate some of the most generous altruists. As a consequence, the tragedy of the commons is predominantly averted due to cooperators that contribute only their "fair share" to the common pool. We also find that larger groups require larger fines for the deterrence of punishment to have the desired prosocial effect.

To give or not to give: Examining the prosocial effects of a 360° video endorsing a clean water charity

This study examines the prosocial effects of virtual reality (VR) technology on donation behavior, using a 360° video documentary supporting the nongovernmental organization Charity: Water. Previous research has shown how VR can be used as a way to evoke empathy towards other people. There is, however, limited research on how this technology can be used to elicit more donations for charitable organizations. This paper aims to investigate whether the use of an emotion-inducing 360° video in VR leads to higher donation rates. We conducted a between-participants experiment and collected data from 150 young adults (age 18–25). Results reveal how VR – and especially empathy – significantly impacts donation intentions. Although donation intentions were found to be higher after watching the VR video, many participants were skeptical about making an actual donation. Implications and future research regarding the capabilities of VR technology in eliciting donations and resolving donation uncertainty are discussed.

Role of 5-HT1A receptors in the basolateral amygdala on 3,4-methylenedioxymethamphetamine-induced prosocial effects in mice

3,4-methylenedioxymethamphetamine (MDMA), a recreational drug, induces euphoric sensations and psychosocial effects, such as increased sociability and empathy. Serotonin, also called 5-hydroxytryptamine (5-HT), is a neurotransmitter that has been associated with MDMA-induced prosocial effects. However, the detailed neural mechanisms remain elusive. In the present study, we investigated whether 5-HT neurotransmission in the medial prefrontal cortex (mPFC) and the basolateral nucleus of amygdala (BLA) is involved in MDMA-induced prosocial effects using the social approach test in male ICR mice. Systemic administration of (S)-citalopram, a selective 5-HT transporter inhibitor, before administration of MDMA failed to suppress MDMA-induced prosocial effects. On the other hand, systemic administration of the 5-HT1A receptor antagonist WAY100635, but not 5-HT1B, 5-HT2A, 5-HT2C, or 5-HT4 receptor antagonist, significantly suppressed MDMA-induced prosocial effects. Furthermore, local administration of WAY100635 into the BLA but not into the mPFC suppressed MDMA-induced prosocial effects. Consistent with this finding, intra-BLA MDMA administration significantly increased sociability. Together, these results suggest that MDMA induces prosocial effects through the stimulation of 5-HT1A receptors in the BLA.

The Role of Oxytocin and Vasopressin in Drug-Induced Reward-Implications for Social and Non-Social Factors.

Drug abuse is a worldwide problem that leads to negative physical, mental, and economic consequences. Although pharmacological strategies for drug addiction management have been widely studied, therapeutic options with high efficacy and a low side-effects profile are still limited. Recently, there has been a growing interest in oxytocin (OT) and vasopressin (AVP) systems as potential therapeutic targets for the treatment of drug abuse. OT and AVP are hypothalamic neuropeptides involved in numerous physiological processes. Additionally, studies show that these neurohormones are highly implicated in the modulation of a wide range of behaviors. Interestingly, ample evidence has shown that both, OT and AVP are able to decrease the consumption of different drugs of abuse, as well as to ameliorate their rewarding and reinforcing effects. Furthermore, OT and AVP have been strongly involved in prosocial effects and social reward. In particular, OT has been shown to be able to shift drug-induced reward into social-induced reward, mainly due to its interaction with the dopaminergic system. This phenomenon is also reflected in the results of clinical trials where intranasal OT shows promising efficacy in managing substance use disorder. Therefore, the aim of this review is to comprehensively characterize the involvement of OT and AVP in the rewarding and other behavioral effects of drugs of abuse in animal models, with a particular highlight on the impact of social factors on the observed effects. Understanding this relationship may contribute to higher drug development success rates, as a result of a more profound and deliberate studies design.

Mindfulness training reduces slippery slope effects in moral decision-making and moral judgment

Extant research has demonstrated the positive intrapersonal effects of mindfulness training. However, the cognitive mechanisms underlying the effects of mindfulness training on interpersonal processes are less clear. Here, we combined a randomized control mindfulness training design with computational approach to moral decision-making and moral judgments. Participants were randomly assigned to a Training group (N = 32) who received an 8-week mindfulness training or a Control group (N = 26) who waited for the same period of time. Before and after the 8-week period, participants completed a moral decision-making task, where they made tradeoff between money for themselves and unpleasant electric shocks to another person, and a moral judgment task, where they evaluated the blameworthiness of someone else’s choices in the same moral decision-making task. Trait mindfulness, as measured by the Five-Facet Mindfulness Questionnaire, significantly increased from the pre- to post-training session for the Training group, but not the Control group, demonstrating the effectiveness of the mindfulness manipulation. For the Control group, participants’ moral preference in both the decision-making task and the judgment task declined over time, exhibiting a “slippery slope” effect. In contrast, for the Training group, mindfulness training prevented moral preferences from declining. Computational modeling revealed that mindfulness training specifically reduced the increase in the weights of money over time in both the decision-making and judgment tasks, thereby curbing the “slippery slope” effects. These findings provide a cognitive account of the prosocial effects of mindfulness training on moral decision-making and moral judgments.

Aromatic Bromination Abolishes Deficits in Visuospatial Learning Induced by MDMA ("Ecstasy") in Rats While Preserving the Ability to Increase LTP in the Prefrontal Cortex.

It has recently been demonstrated that aromatic bromination at C(2) abolishes all typical psychomotor, and some key prosocial effects of the entactogen MDMA in rats. Nevertheless, the influence of aromatic bromination on MDMA-like effects on higher cognitive functions remains unexplored. In the present work, the effects of MDMA and its brominated analog 2Br-4,5-MDMA (1 mg/kg and 10 mg/kg i.p. each) on visuospatial learning, using a radial, octagonal Olton maze (4 × 4) which may discriminate between short-term and long-term memory, were compared with their influence on in vivo long-term potentiation (LTP) in the prefrontal cortex in rats. The results obtained indicate that MDMA diminishes both short- and long-term visuospatial memory but increases LTP. In contrast, 2Br-4,5-MDMA preserves long-term visuospatial memory and slightly accelerates the occurrence of short-term memory compared to controls, but increases LTP, like MDMA. Taken together, these data are consistent with the notion that the modulatory effects induced by the aromatic bromination of the MDMA template, which abolishes typical entactogenic-like responses, might be extended to those effects affecting higher cognitive functions, such as visuospatial learning. This effect seems not to be associated with the increase of LTP in the prefrontal cortex.

Pain control based on oscillatory brain activity using transcranial alternating current stimulation: An integrative review.

Developing effective tools and strategies to relieve chronic pain is a high-priority scientific and clinical goal. In particular, the brain regions related to pain processing have been investigated as potential targets to relieve pain by non-invasive brain stimulation (NIBS). In addition to elucidating the relationship between pain and oscillatory brain activity, transcranial alternating current stimulation (tACS), which can non-invasively entrain oscillatory brain activity and modulate oscillatory brain communication, has attracted scientific attention as a possible technique to control pain. This review focuses on the use of tACS to relieve pain through the manipulation of oscillatory brain activity and its potential clinical applications. Several studies have reported that tACS on a single brain reduces pain by normalizing abnormal oscillatory brain activity in patients with chronic pain. Interpersonal tACS approaches based on inter-brain synchrony to manipulate inter-brain communication may result in pain relief via prosocial effects. Pain is encoded by the spatiotemporal neural communication that represents the integration of cognitive, emotional-affective, and sensorimotor aspects of pain. Therefore, future studies should seek to identify the pathological oscillatory brain communication in chronic pain as a therapeutic target for tACS. In conclusion, tACS could be effective for re-establishing oscillatory brain activity and assisting social interaction, and it might help develop novel approaches for pain control.

Does language make people more prosocial? The influence of Mandarin proficiency on donation behavior.

Both the effects of language proficiency on individual outcomes, and the influencing factors of individual donation arouse wide concern, but researchers have hardly probed into the relationship between language proficiency and donation behavior. Using the data from the 2012 China General Social Survey (CGSS) and the binary logistic regression model as the benchmark model, this study empirically examines the influence and mechanism of language proficiency on donation behavior. It is revealed that Mandarin proficiency has significant positive influence on individual donation behavior. According to the results of the variable substitution practice and the instrumental variable regression based on the two-stage least square model, the above conclusion remains robust. The heterogeneity test shows that Mandarin proficiency of male, southern and rural residents has more obvious impact on donation behavior. The multiple intermediary effect test indicates that perceived social responsibility and subjective social status partially mediate the relationship between Mandarin proficiency and donation behavior. Therefore, it indicates that language proficiency has a prosocial effect, which makes people more prosocial. This study contributes to the literature on donation behavior by examining the influencing mechanism of Mandarin proficiency on individual donation, and further the effects of language proficiency on individual outcomes, thereby providing theoretical and empirical support for the formulation of policies for the promotion of Mandarin and social donation in China.

Awe's prosocial effect: the mediating role of the small self and the authentic self

Awe's prosocial effect: the mediating role of the small self and the authentic self

Costly Commitments “Under His Eye”: Reconceptualizing the Costly Signaling Theory of Religion

AbstractCostly signaling theory of religion has been proposed to explain the evolutionary adaptiveness of religion in general and, specifically, its prosocial effects, including the relative longevity of religious communes vis-à-vis their secular counterparts. This article focuses on two crucial aspects of this relationship: the features and functions of signals and the mechanism through which signaling translates into enhanced prosociality. It identifies some of the key factors of the costliness of behavior and distinguishes between religious and secular signals, arguing that only the latter serve to broadcast commitment. The role of religious signals, instead, might be to stimulate the supernatural watching (“Under His Eye”) mechanism: enhancing supernatural sanctions beliefs and providing a setting in which implicit prosocial responses are triggered. The relative absence of this mechanism in secular communities may explain their shorter life spans. A link is thus established between the costly signaling and supernatural punishment theories of religious behavior.

Do Violent Video Games Reduce Aggression? The Roles of Prosociality and Cooperation

ABSTRACT Video games have become an integral part of people’s daily life. Though detrimental outcomes of negative game factors have been demonstrated across previous studies, the effects of prevalently-exist positive game factors, for example, prosociality and cooperation, remain unclear. This study examined the effects of positive game factors in violent video games on aggressive behavior by exploring prosociality and cooperation. Based on the General Learning Model, Experiment 1 examined the effect of prosociality on aggressive behavior and the mediating role of perceived violence in the game. Experiment 2 examined the relation between cooperation in violent video games and aggressive behavior, the mediating role of hostile interpretation bias, and the moderating role of the game result. The results of Experiment 1 showed that prosociality did not influence aggressive behavior. The results of Experiment 2 showed that cooperation decreased aggressive behavior. Additionally, Experiment 2 also found that the hostile interpretation bias mediated the relation between cooperation and aggressive behavior, and win/lose results moderate the relationship between cooperation and aggressive behavior. Considering the questions raised by post hoc sensitivity analysis, future studies could investigate the effects of positive game factors in violent video games on aggressive behavior with a larger sample size.

Effects of repeated lysergic acid diethylamide (LSD) on the mouse brain endocannabinoidome and gut microbiome.

Psychedelics elicit prosocial, antidepressant and anxiolytic effects via neuroplasticity, neurotransmission and neuro-immunomodulatory mechanisms. Whether psychedelics affect the brain endocannabinoid system and its extended version, the endocannabinoidome (eCBome) or the gut microbiome, remains unknown. Adult C57BL/6N male mice were administered lysergic acid diethylamide (LSD) or saline for 7 days. Sociability was assessed in the direct social interaction and three chambers tests. Prefrontal cortex and hippocampal endocannabinoids, endocannabinoid-like mediators and metabolites were quantified via high-pressure liquid chromatography with tandem mass spectrometry (HPLC-MS/MS). Neurotransmitter levels were assessed via HPLC-UV/fluorescence. Gut microbiome changes were investigated by 16S ribosomal DNA sequencing. LSD increased social preference and novelty and decreased hippocampal levels of the N-acylethanolamines N-linoleoylethanolamine (LEA), anandamide (N-arachidonoylethanolamine) and N-docosahexaenoylethanolamine (DHEA); the monoacylglycerol 1/2-docosahexaenoylglycerol (1/2-DHG); the prostaglandins D2 (PGD2 ) and F2α (PGF2α ); thromboxane 2 and kynurenine. Prefrontal eCBome mediator and metabolite levels were less affected by the treatment. LSD decreased Shannon alpha diversity of the gut microbiota, prevented the decrease in the Firmicutes:Bacteroidetes ratio observed in saline-treated mice and altered the relative abundance of the bacterial taxa Bifidobacterium, Ileibacterium, Dubosiella and Rikenellaceae RC9. The prosocial effects elicited by repeated LSD administration are accompanied by alterations of hippocampal eCBome and kynurenine levels, and the composition of the gut microbiota. Modulation of the hippocampal eCBome and kynurenine pathway might represent a mechanism by which psychedelic compounds elicit prosocial effects and affect the gut microbiome.

The Effect of Limosilactobacillus reuteri on Social Behavior Is Independent of the Adaptive Immune System.

Gut microbes can modulate almost all aspects of host physiology throughout life. As a result, specific microbial interventions are attracting considerable attention as potential therapeutic strategies for treating a variety of conditions. Nonetheless, little is known about the mechanisms through which many of these microbes work. Recently, we and others have found that the commensal bacterium Limosilactobacillus reuteri (formerly Lactobacillus reuteri) reverses social deficits in several mouse models (genetic, environmental, and idiopathic) for neurodevelopmental disorders in a vagus nerve-, oxytocin-, and biopterin-dependent manner. Given that gut microbes can signal to the brain through the immune system and L. reuteri promotes wound healing via the adaptive immune response, we sought to determine whether the prosocial effect mediated by L. reuteri also depends on adaptive immunity. Here, we found that the effects of L. reuteri on social behavior and related changes in synaptic function are independent of the mature adaptive immune system. Interestingly, these findings indicate that the same microbe (L. reuteri) can affect different host phenotypes through distinct mechanisms. IMPORTANCE Because preclinical animal studies support the idea that gut microbes could represent novel therapeutics for brain disorders, it is essential to fully understand the mechanisms by which gut microbes affect their host's physiology. Previously, we discovered that treatment with Limosilactobacillus reuteri selectively improves social behavior in different mouse models for autism spectrum disorder through the vagus nerve, oxytocin reward signaling in the brain, and biopterin metabolites (BH4) in the gut. However, given that (i) the immune system remains a key pathway for host-microbe interactions and that (ii) L. reuteri has been shown to facilitate wound healing through the adaptive immune system, we examined here whether the prosocial effects of L. reuteri require immune signaling. Unexpectedly, we found that the mature adaptive immune system (i.e., conventional B and T cells) is not required for L. reuteri to reverse social deficits and related changes in synaptic function. Overall, these findings add new insight into the mechanism through which L. reuteri modulates brain function and behavior. More importantly, they highlight that a given bacterial species can modulate different phenotypes (e.g., wound healing versus social behavior) through separate mechanisms.