Effect Of Probiotic Preparation Research Articles

Effect of probiotic preparation on goat milk quality

The effect of the experimental probiotic drug "Plantarum" on the physico-chemical and microbiological parameters of milk of crossbred dairy goats was studied. This preparation was developed in the laboratory of microbiology of milk and dairy products of the Siberian Research Institute of Cheese Making of the Federal Altai Scientific Center for Agrobiotechnology. The preparation includes pure cultures of probiotic strains of lacto- and propionic acid bacteria from the "Siberian collection of microorganisms". Scientific and economic experiment was conducted in 2018-2020 on the territory of the Pervomaisky district of the Altai Territory. The objects of the study were crossbred dairy goats. In order to evaluate the effect of the experimental probiotic drug "Plantarum" on the milk quality of the experimental animals, four groups (20 animals in each group) of pregnant goats were formed: one control and three experimental. The goats of the experimental groups were fed the probiotic drug "Plantarum" in addition to the basic diet at the doses of 0.4, 0.6, and 0.8 ml/kg of body weight/day. The results showed that the content of the mass fraction of fat and protein in the milk samples of all experimental groups were within the norm for this type of animals regulated by GOST 32940-2014. It should be noted that the use of different doses of probiotic increased the fat mass fraction in milk from 0.06 to 0.13% and the protein mass fraction by 0.12% relative to the control. Physicochemical parameters (dry skimmed milk residue, titratable acidity, somatic cells) of milk of the experimental goats were within normal limits; no significant differences between the groups were found. There was a tendency to reduce the bacterial contamination of milk in the experimental groups compared to the control when determining the number of mesophilic aerobic and facultatively anaerobic microorganisms.

Antimicrobial and therapeutic effect of probiotics in cases of experimental purulent wounds

Probiotics based on bacteria of the genus Bacillus with a multifactorial mechanism of action are considered as a possible alternative to antibiotics in the treatment of purulent wounds. The aim of the study was to determine the antimicrobial and therapeutic effect of the Arederma probiotic preparation containing probiotic strains of the genus Bacillus in an experimental model of a purulent wound in animals. The antimicrobial efficacy of the probiotic against test strains and clinical isolates of pathogenic and opportunistic microorganisms was studied using the method of delayed antagonism. Staphylococcus aureus ATCC 6538 and Streptococcus pyogenes K-7 were used to model a purulent wound. From the surface of the wounds, bacteria of Staphylococcus, Streptococcus, Enterobacteriaceae, Pseudomonas genera and Enterobacteriaceae family were sown on appropriate selective media for the cultivation and enumeration of different groups of microorganisms by generally accepted microbiological research methods. The formation of a purulent wound in rabbits caused by mechanical skin damage and subsequent double infection with Staphylococcus aureus ATCC 6538 and Streptococcus pyogenes K-7 strains was accompanied with a pronounced inflammatory process, necrosis, the formation of purulent exudate and general intoxication. Representatives of the genera Staphylococcus and Streptococcus, microscopic fungi and, to a lesser extent, members of the family Enterobacteriaceae and Pseudomonas were found on the surface of purulent wounds, which confirmed the development of the infectious-inflammatory process. Treatment of purulent wounds with a suspension of probiotic preparation once a day for 4 days led to their faster healing (gradual attenuation of the inflammatory process, reduction of edema and discharge, as well as their disappearance) compared with untreated purulent wounds (control). Representatives of the Staphylococcus and Streptococcus genera, as well as microscopic fungi, presented in purulent wounds treated with probiotic preparation in much smaller numbers than in the control, and bacteria of the Pseudomonas genus and the Enterobacteriaceae family were not detected at all. The effective antimicrobial effect of this probiotic preparation against opportunistic and pathogenic microorganisms was confirmed by in vitro studies. Therefore, the Arederma probiotic preparation showed an effective therapeutic and antimicrobial effect in the experimental model of a purulent wound in animals, so it can be recommended for further preclinical and clinical studies.

Antimutagenic activity of probiotic drugs on the example of Drosophila

Probiotic preparations were obtained on the basis of cryopreserved forms of pure cultures of L. plantarum and B. subtilis using freeze drying. The antimutagenic activity of the obtained probiotic preparations was investigated in relation to 1%, 0.1% and 0.01% concentrations of cobalt sulfate using the example of dominant lethal mutations of Drosophila. A positive antimutagenic effect of probiotic preparations was shown in relation to 0.1% and 0.01% concentrations of CoSO4 with an exposure duration of at least 3 days.

Effect of probiotic preparations (EM) on productive characteristics, carcass composition, and microbial contamination in a commercial broiler chicken farm

This experiment evaluated the effect of Pro-Biotyk (Em-15) and EMFarma™ probiotics on body weight, feed intake and conversion, carcass traits, and microbial contamination in a poultry house. The probiotic preparations caused a nonsignificant increase in body weight (42 days), feed intake, and feed conversion ratio (1–42 days) and a nonsignificant decrease in chicken mortality from 4 weeks of rearing. Chickens exposed to probiotics did not differ significantly in preslaughter body weight, carcass weight, dressing percentage, and the content of carcass components. The carcasses from experimental chickens had a lower percentage of breast muscle, leg muscle, abdominal fat, and neck, as well as a higher percentage of skin with subcutaneous fat, wings, and remainder of carcasses compared with the carcasses from control birds. The probiotic preparations used in this study were highly effective as auxiliary disinfectants.

ОСОБЛИВОСТІ ГЕМАТОЛОГІЧНОГО І БІЛКОВОГО ПРОФІЛЮ ТА СТАНУ НЕСПЕЦИФІЧНОЇ РЕЗИСТЕНТНОСТІ ОРГАНІЗМУ ПТИЦІ ПРИ ЗАСТОСУВАННІ ПРОБІОТИКА ТА НАНОКОБАЛЬТУ

The obtained results on the state of the immune system in conjunction with these dynamics oferythrocyte number and total hemoglobin, changes in protein gramme convincingly testify adaptogenic effect of probiotics and cobalt nanoparticles at a dose of 0.08 mg/kg of body weight on the state of nonspecific resistance and protein metabolism in the body of the research poultry.However alimentary chronic entering of probiotic togetherwith cobalt nanoparticles at a dose of 0.80 mg/kg at the late terms of research leads to such changes in protein grammewhich are typical signs of protein synthetic liver function (with hypoalbuminemia) and the development of immunosuppression (for increased levels of seromucoid).According to results of chronic toxicological experiment on laying hensis possible to assert about no harmful actions of probiotics and probiotic along with the addition of cobalt in the form of salts and nanoparticles at a dose of 0.08 mg/kg of body weight, which makes it really as biotic.Adaptogenic effect of probiotic preparations and additives of cobalt nanoparticles at a dose of 0.08 mg/kg of body weight at alimentary earnings to hens body consists in strengthening of immune reactions along with the restoration parameters of erythropoiesis and protein exchange.

Effects of Probiotic Preparation on Metabolic Activity of Enteric Microbiota in Children with Atopic Dermatitis

ABSTRACTThe aim of the research was to evaluate the amount and kind of short-chain fatty acids (SCFA) in faeces of children with atopic dermatitis (AD) and to show whether the supplementation of children's diet with a probiotic preparation affects SCFA composition. Children were administrated with probiotic preparation during 3 months in daily dose of 5x109 cells or placebo. The faeces samples were analyzed before the administration of probiotics or placebo, after finishing of their in-take, and then 5 months later. SCFA concentration was measured with the use of HPLC method.The average concentration of the lactic and acetic acid before administration of probiotics was 1.11%/g of faeces. It increased at about 20% after finishing the probiotic supplementation, and then at about 5% (5 months later). The concentration of the propionic acid was higher at about 18% after 3 months of probiotic administration, and increased on average at about 15% (5 months later). The butyrate acid was present only in a few samples of faeces, irrespective of the fact whether the children were given the probiotic preparation or placebo, and its concentration was from 0.02%/g to 0.64%/g of faeces.Our study has shown that probiotic administration affects the SCFA concentration, in general lactic, acetic and propionic acids.