Low multiplication ratio, long growth and development duration contribute to the long conventional breeding cycle of yams. Moreover, little is known about the effect of pot size and transplanting date on the yield of introduced semi-autotrophic hydroponics (SAH) derived micro-tubers of white yam (Dioscrearotundata Poir). This study was carried out to evaluate the effect of pot size and transplanting date on tuber production of introduced SAH micro-tuber derived white yam var TDr9719177 in shed-house conditions. The SAH materials were introduced from the International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria. The trial involved two factors including two different pot sizes (38.5×28.5cm and 29.5×23.0cm) and two transplanting dates (September, 2020 and October, 2020). The results showed that micro-tubers grown in the big pot (38.5×28.5cm) significantly out yielded those established in the small pot (29.5×23.0cm). Transplanting in September produced heavier fresh tubers per plant (123.4g)and fresh tuber weight per tuber (88.8g) than transplanting in October, which had 17.4g and 14.1g, respectively. The mean difference of fresh tuber weight per plant between the transplanting pots was 68.4%, and between the transplanting dates was 85.9%. The mean difference of weight per tuber between the transplanting pots was 74.2%, and between the transplanting dates was 84.1%. The results suggested the relevance of early transplanting of healthy seedlings from SAH derived micro-tubers that could be exploited for deployment and promotion to seed yam producers for increased yam productivity. It also depicted the relevance of transplanting SAH micro-tubers in big container that promotes higher tuber production that could be exploited for the rapid multiplication of early generation seeds in yam breeding.