Effect Of Ozone Treatment Research Articles

Investigating the effect of ozone treatment on microbiological, proteolytic and lipolytic characteristics of brined ultrafiltered cheese during ripening

Investigating the effect of ozone treatment on microbiological, proteolytic and lipolytic characteristics of brined ultrafiltered cheese during ripening

Effects of Ozone Treatments on in vitro Seed Germination of Ruscus aculeatus, Ruscus hypoglossum and Danae racemosa

Ruscus aculeatus, R. hypoglossum and Danae racemosa are cut foliage and valuable for ornamental plants, they are also very valuable in terms of their medicinal aromatic properties. Propagating these plants using their seeds are very challenging due to deep dormancy and required pre-treatments before sowing. Ozone is a colorless gas with a pungent odor, made up of three oxygen atoms and it offers eco-friendly solution to break dormancy in seed. In this study, stored seeds for four years were treated to ozone gas for 0, 15 and 30 minutes using a ozone generator has the capacity to produce 6 grams of ozone per hour. Then the seed cultured in Petri dishes containing Murashige and Skoog medium without plant growth regulators. In vitro seed germination rates were recorded at 30, 40, 50 and 60 days after culture initiation. According to statistical analysis effects of species, duration of ozone treatments and interaction of species and duration of ozone treatments on in vitro germination rates of seeds were statistically significant. The highest in vitro germination rates with 42%, 28% and 24% were recorded at 30 min ozone treatment in R. aculeatus, R. hypoglossum and D. racemosa respectively. These results indicate that ozone application positively affects the germination of plant seeds.

СНИЖЕНИЕ ЖЁСТКОСТИ ОРОСИТЕЛЬНОЙ ВОДЫ ОЗОНИРОВАНИЕМ

Abstract. The article presents the research on the effect of ozone treatment on the hardness of irrigation water. The effect of hard water on plant growth and development has been analyzed. It has been revealed that excessive magnesium and calcium ions in irrigation water lead to the suppression of agricultural crops and a decrease in their yield. To conduct laboratory studies, water was taken from a natural reservoir located near the village of Gvazda, Buturlinovsky District, Voronezh Region, which is used by local farmers to irrigate agricultural land. The sample was divided into two parts, one of which was subjected to ozone treatment, and the second was a control variant. Laboratory studies have established that the total water hardness in the natural reservoir was 21 dH, which corresponds to 7.5 mg eq / l. This level of hardness is excessive and will negatively affect plants. Ozonation at an average ozone concentration in the ozone-air mixture of 4.67 mg/m3 allowed to reduce the total water hardness to 14 dH, which is comparable to 5 mg eq/l. Intensive water softening occurred at ozone treatment doses of up to 840 mg min/m3. Consequently, ozonation reduces the content of polyvalent metal ions, which in high concentrations have a negative effect on plants. Modern compact, energy-efficient, high-performance ozonizers can be easily integrated into the design of sprinkler machines. Softer water will have a positive effect on the efficiency of irrigation and the reliability of irrigation systems. In addition, ozone water treatment will have a bactericidal and disinfectant effect, which will prevent the development of various diseases and pests.

Effect of ozone treatment on phenylpropanoid metabolism in harvested cantaloupes.

Phenylpropanoid metabolism plays an important role in cantaloupe ripening and senescence, but the mechanism of ozone regulation on phenylpropanoid metabolism remains unclear. This study investigated how ozone treatment modulates the levels of secondary metabolites associated with phenylpropanoid metabolism, the related enzyme activities, and gene expression in cantaloupe. Treating cantaloupes with 15mg/m3 of ozone after precooling can help maintain postharvest hardness. This treatment also enhances the production and accumulation of secondary metabolites, such as total phenols, flavonoids, and lignin. These metabolites are essential components of the phenylpropanoid metabolic pathway, activating enzymes like phenylalanine ammonia-lyase, cinnamate 4-hydroxylase, 4CL, chalcone synthase, and chalcone isomerase. The results of the transcriptional expression patterns showed that differential gene expression related to phenylpropanoid metabolism in the peel of ozone-treated cantaloupes was primarily observed during the middle and late storage stages. In contrast, the pulp exhibited significant differential gene expression mainly during the early storage stage. Furthermore, it was observed that the level of gene expression in the peel was generally higher than that in the pulp. The correlation between the relative amount of gene changes in cantaloupe, activity of selected enzymes, and concentration of secondary metabolites could be accompanied by positive regulation of the phenylpropanoid metabolic pathway. Therefore, ozone stress induction positively enhances the biosynthesis of flavonoids in cantaloupes, leading to an increased accumulation of secondary metabolites. Additionally, it also improves the postharvest storage quality of cantaloupes.

Ozone treatment of meat and meat products: a review

Ozone treatment is a non-thermal method for disinfection; ozone is a powerful oxidizing agent that has been shown to be effective in reducing microbial load, extending the shelf life of meat products. This mini-review covers the analysis of the antimicrobial ozone activity in different meats (beef, poultry, pork, seafood, etc.), emphasizing the ozone application methods (liquid or gaseous phase), the applied concentrations and contact times and the effects of ozone treatment on meat quality, safety, and sensory properties. It has been demonstrated that ozone is effective against a broad range of microorganisms, including both Gram-positive and Gram-negative bacteria, spores, and vegetative cells. The efficacy of ozone depends on various factors, such as concentration, type of treatment, temperature, and presence of organic material. Ozone treatment, known for its rapid decomposition and lack of residue, provides an environmentally friendly alternative to traditional chemical sanitizers. Ozone treatments exhibit promising results in enhancing the safety and extending the shelf life of meat products. According to the findings, the application of ozone is an effective technology for prolonging the shelf life of different types of meats and meat products, requiring careful establishment of conditions on a case-by-case basis.

Effect of ozone treatment on thermo tolerant Campylobacter contaminated poultry products retailed at Baghdad markets/Iraq

Effect of ozone treatment on thermo tolerant Campylobacter contaminated poultry products retailed at Baghdad markets/Iraq

ВЛИЯНИЕ ОЗОНА НА КОРРОЗИОННОЕ ПОВЕДЕНИЕ МЕТАЛЛОВ И СПЛАВОВ

Research on the effect of ozone treatment on metal corrosion is presented in the article. The results of experiments on changes in the state of metals and steels when they are in aggressive environments in the presence of ozone are analyzed. In most cases, the ozone-air mixture has a negative effect on various metals and welded joints, increasing the rate of corrosion. Considering that recently the popularity of ozone technologies in all industries has been steadily growing, the massive use of ozone in agriculture is a matter of time. Therefore, there is a need to conduct research aimed at studying the corrosion resistance of various steels and alloys used in the agricultural sector. It has been analytically established that one of the most accessible alloying elements resistant to ozone treatment is chromium. However, its content in steel grades Steel 08kp, Steel 45, 45X, Steel 60S2, which are most widely used in agricultural engineering, ranges from 0.1 to 1.1%. This chromium content may be insufficient and therefore similar studies need to be carried out. It was established that the most common Steel 08kp showed sufficient resistance to aggressive environments in the presence of ozone as a result of the experiment, quite comparable with more expensive alloys. At the same time, the introduction of ozone into a solution of 70% acetic acid reduced the weight loss per unit area of steel grades Steel 08kp, Steel 45, 45X, Steel 60S2 by 2...7 times. This suggests that ozone blocks metal atoms and prevents exposure to aggressive environments.

Effect of ozone treatment on the decay and cell wall metabolism during the postharvest storage of cantaloupe

Cantaloupe is a popular summer fruit with immense market potential in China. However, the cantaloupe fruits generally soften rapidly after harvest, which dramatically reduces their economic value. The ozone treatment has been found to be effective in delaying the softening of cantaloupe fruit. The results indicated that ozone treatment significantly inhibited the mycelial growth of Fusarium hypha. The ozone treatment was able to delay the decline of total soluble solids, lower the respiration rate, and maintain the fruit's firmness without damaging fruit quality. The study also indicated that the activity and gene expression of cell wall degrading enzymes such as polygalacturonase (PG), pectin methylesterase (PME), β-galactosidase (β-gal), cellulose (Cx), and α-l-arabinofuranosidase (α-l-Af), were inhibited under ozone treatment, which also suppressed the degradation of cell wall components associated with fruit softening (protopectin, soluble pectin, cellulose, and hemicellulose). These changes may contribute to the maintenance of cell wall components, thereby delaying the fruit softening. In conclusion, these results suggested that ozone can improve the fruit quality of cantaloupe by suppressing postharvest fungal diseases and delaying fruit softening, which provides a theoretical basis for further research on cell wall metabolism during cantaloupe storage.

Effects of Ozone Treatment on Postharvest Mucor Rot of Codonopsis pilosula Caused by Actinomucor elegans

Fresh Codonopsis pilosula is highly susceptible to fungus contamination during post-harvest storage, which not only compromises the quality of C. pilosula but also contributes mycotoxin contamination, posing a significant threat to human health. Studies have indicated that ozone treatment can inhibit post-harvest diseases in fruits and vegetables. The impact of ozone treatment on the disease incidence, disease severity index, and weight loss rate of the fresh C. pilosula infected with Actinomucor elegans was investigated through the spray inoculation of A. elegans on C. pilosula tissues. Changes in the main active ingredients of C. pilosula after ozone treatment were analyzed, and the effects of ozone treatment on the integrity of cell membranes in C. pilosula tissue and reactive oxygen species (ROS) metabolism were studied. The results showed that ozone treatment had a significant inhibitory effect on the A. elegans-induced mucor rot in C. pilosula, significantly reducing the incidence of the disease. Compared with the control group, the ozone-treated group maintained the effective components of C. pilosula well. Furthermore, ozone treatment reduced the cell membrane permeability and Malondialdehyde (MDA) content in C. pilosula, significantly increased the activity of antioxidant enzymes in the ROS metabolism pathway, prevented oxidative stress caused by the accumulation of ROS in C. pilosula tissues, and maintained the integrity of cell membranes.

OZONE A NOVEL INTERVENTION IN PERIODONTAL THERAPY - AN OVERVIEW

Ozone has antimicrobial, immuno-stimulatory, anti-hypoxic, bioenergetic, and biosynthetic properties. It is inexpensive, minimally invasive and lacks adverse effects. It is a powerful tool for treating bacterial, fungal, and viral infections, as well as age-related macular degeneration, ischemic disorders, ocular disorders, orthopaedic diseases, haematological diseases, neurodegenerative diseases, pulmonary diseases, renal diseases, dermatological diseases and periodontal diseases. The use of ozone is well indicated in all stages of gingival and periodontal diseases because of its beneficial biological effects, its anti-microbial activity, and its healing and tissue regeneration properties. The effects of ozone treatment in any form (gaseous, water, or oil) have been investigated and analysed with potential exceptional results in the management of periodontal diseases. This review article outlines the clinical applications of ozone therapy in periodontal diseases.

Isolation and Identification of Pathogens Causing Blue Mold of Lanzhou Lily during Postharvest Storage and Control of Disease and Mycotoxin Accumulation by Ozone Treatment.

Blue mold (penicilliosis) is a common disease of Lanzhou lily (Lilium davidii var. willmottiae) during postharvest storage, which not only seriously affects the appearance and reduces the quality of lily bulbs, but also leads to the accumulation of mycotoxins in rotten lily tissues, seriously endangering human health. Therefore, it is of great significance to clarify the main isolates causing postharvest blue mold of fresh Lanzhou lily and put forward effective measures to control the disease caused by these pathogens. In this study, pathogens were isolated and purified from the naturally diseased blue-mold tissue of Lanzhou lily, and then morphological and molecular biology techniques were applied to identify the isolates, verify the pathogenicity, determine the disease index and disease incidence, and finally, to analyze the control effect of ozone treatment on the blue mold of lily scale and mycotoxin accumulation. The results indicated that the main isolates causing postharvest blue mold of lily were Talaromyces adpressus, Penicillium gladioli, T. calidominioluteus, and P. polonicum. The pathogenicity test showed that P. gladioli and P. polonicum had a higher disease index than T. calidominioluteus and T. adpressus. Ozone treatment significantly reduced the incidence of disease caused by P. gladioli and P. polonicum, and effectively controlled the accumulation of patulin. This study characterized the main pathogens causing blue mold of postharvest Lanzhou lily during storage, and confirmed ozone application has a significant inhibitory effect on blue mold development and patulin accumulation in Lanzhou lily, which could be helpful in commercially controlling blue mold of postharvest Lanzhou lily during storage.

Ozone treatment modulates reactive oxygen species levels in kiwifruit through the antioxidant system: Insights from transcriptomic analysis

Owing to its easy decomposition and residue-free properties, ozone has been used as an effective and environmentally friendly physical preservation method for maintaining the post-harvest quality of fruits. This study aimed to investigate the effects of ozone treatment on the levels of oxidative stress markers and the status of the antioxidant defense system in refrigerated kiwifruit. Additionally, the study aimed to identify the differences in gene expression levels and potential regulatory effects from the transcriptional level. The results showed that ozone treatment reduced the respiration rate, maintained the fruit hardness and storage quality, and inhibited the ripening and senescence of kiwifruit. Ozone treatment activated antioxidant enzymes (superoxide dismutase, peroxidase, and catalase) and ascorbate-glutathione cycle to prevent the increase of reactive oxygen species levels (H2O2, O2−•) and malonaldehyde content, maintaining lower membrane lipid peroxidation and reactive oxygen species (ROS) accumulation than the control treatment. Further analysis showed that the regulatory ability of ROS in kiwifruit treated with ozone was not only related to the synergistic effect of enzyme activity and gene expression related to the antioxidant oxidase system and the ascorbate-glutathione (ASA-GSH) cycle but also related to downstream hormone signaling. This study provides a foundation for understanding the potential effects of ozone treatment on the antioxidant cycle of kiwifruit and provides valuable insights into the molecular basis and related key genes involved in regulating ROS to delay aging in kiwifruit.

Elucidating the molecular mechanisms of ozone therapy for neuropathic pain management by integrated transcriptomic and metabolomic approach.

Introduction: Neuropathic pain remains a prevalent and challenging condition to treat, with current therapies often providing inadequate relief. Ozone therapy has emerged as a promising treatment option; however, its mechanisms of action in neuropathic pain remain poorly understood. Methods: In this study, we investigated the effects of ozone treatment on gene expression and metabolite levels in the brainstem and hypothalamus of a rat model, using a combined transcriptomic and metabolomic approach. Results: Our findings revealed significant alterations in key genes, including DCST1 and AIF1L, and metabolites such as Aconitic acid, L-Glutamic acid, UDP-glucose, and Tyrosine. These changes suggest a complex interplay of molecular pathways and region-specific mechanisms underlying the analgesic effects of ozone therapy. Discussion: Our study provides insights into the molecular targets of ozone treatment for neuropathic pain, laying the groundwork for future research on validating these targets and developing novel therapeutic strategies.

Effects of ozone treatment to the levels of neurodegeneration biomarkers after rotenone induced rat model of Parkinson’s disease

The study investigated the effects of ozone treatment on the neurodegeneration of stereotaxic rotenone-induced parkinson's disease (PD) model. The model was confirmed using the apomorphine rotation test. α-synuclein, amyloid-β, Tau, phosphorylated Tau, as well as tyrosine hydroxylase(+), nNOS(+), and glial cell counts were used to evaluate neurodegeneration in the substantia nigra pars compacta and ventral tegmental area. The experiment involved 48 Sprague-Dawley rats divided into four groups: dimethyl sulfoxide (DMSO), DMSO with ozone (O), DMSO/rotenone (R), and D/R/O. Ozone treatment significantly improved tissue α-synuclein level and TH+, nNOS+, and glial cell counts compared to the rotenone-only group. The study suggests that ozone treatment may have beneficial effects on PD biomarkers in the rotenone model. Further studies on ozone dosage, duration, and administration methods in humans could provide more evidence for its potential use in Parkinson's disease treatment.

Effect of ozone treatment on microbial and quality alteration of onions during 2 months storage.

Storing and preserving onion using pesticides is common in Brebes District. The study determined the use of ozone to reduce microorganisms and to maintain the onion quality during months of post-harvesting. There were three chambers with 2.5 m3 in size, and with a capacity of 20 kg onion used for this study. Each chamber received different treatment for 2 months; chamber I: no treatment; chamber II: pesticide; chamber III: ozone. The ozone treatment maintained better temperature and humidity for preservation. Ozone treatment was also observed to maintain low level of mass damage (1.8%) as compared to control and pesticide (6.5% and 2.4%). The protein, ash, fat and vit B1 of onions were remained high in ozone-treated chamber. Total Plate Count (CFU/mL) and the Mold Yeast Count (colonies/mL) decreased on day 30 and 60 after pesticides and ozone treatment. This study showed the importance of ozone treatment to maintain better quality of onion after months of storage as compared to pesticide treatment. Therefore, ozone can be a potential use to replace the pesticide for preservation at post-harvesting.

Experimental evaluation of the effect of ozone treatment on the oxidation and removal of dry soot deposits of the exhaust gas recirculation system

The integration of alternative energy sources as a replacement for fossil fuels across various industrial sectors, including power generation, emergency systems, or marine applications, is uncertain. As a result, the utilization of traditional fuels is not anticipated to be fully phased out in the near future. To address this, new technologies, such as those that employ oxidising atmospheres, have been explored as a means to enhance the pollution control capabilities of existing technologies, as the Exhaust Gas Recirculation (EGR) system. In this regard, the present study has assessed the efficacy of ozone atmosphere exposure in mitigating the formation of undesired fouling deposits within the system, with the aim of facilitating more efficient operation of EGR devices and extending their service life. To this end, dry soot samples have been exposed to various ozone atmospheres at different temperatures and ozone concentrations through the utilization of an experimental test bench. The oxidation potential of these atmospheres has been evaluated through the analysis of the deposit mass loss. Likewise, confocal microscopy techniques have been employed to obtain the 3D topography of the fouling samples before and after the ozone treatment, allowing the assessment of the deposit thickness reduction, as well as the surface roughness variation. Additionally, thermogravimetric analysis has been conducted to examine the effects of the oxidation processes on fouling samples composition. The findings of this study have revealed that ozone atmospheres have been effective in reducing deposit mass at ozone treatment temperatures above 100 °C. The reduction in mass has reached 78.5% and 91.8% with treatment temperature of 140 °C with ozone concentrations of 30 gO3/m³ and 50 gO3/m³, respectively. It has also been established that treatment conditions with ozone concentrations of 30 gO3/m³ and 50 gO3/m³ are effective in reducing the thickness of deposits even at intermediate treatment temperatures, resulting in a thickness reduction of 78.6% and 81.1% at 80 °C, respectively. Additionally, it has been observed that the ozone exposure leads to the increase in the proportion of volatile material within the deposit.

Effects of adjuvant application of corticosteroid and ozone after ultrasound-guided puncture and lavage for the treatment of rotator cuff calcific tendinitis: study protocol for a non-inferiority randomized controlled trial

BackgroundSteroid injection after percutaneous irrigation of calcific deposits is a common method for the treatment of rotator cuff calcific tendinitis (RCCT). However, steroids may prevent calcification resorption and cause potentially irreversible damage to tendons. Recent studies have confirmed the positive effects of ozone injection in shoulder tendinopathies, but no RCCTs have been reported. Thus, our study aims to evaluate the non-inferiority of ozone versus steroid injection.MethodsThis is a prospective, randomized, parallel control and non-inferiority trial. A total of 100 patients with unilateral symptomatic RCCT will be enrolled and randomized in a 1:1 ratio to two groups: ultrasound-guided injection with ozone or corticosteroid. The primary outcome is the numeric rating scale for pain (NRS) at 1 week and 3 months following the procedure. Secondary outcomes include a multi-dimensional evaluation of shoulder disability and quality of life improvement, the degree of calcification absorption after treatment, and the number of multiple treatments.DiscussionThe results of this study will provide short-term and long-term evidence for the effectiveness of ozone treatment in RCCT in relieving pain or improving shoulder function.Trial registrationChinese Clinical Trial Registry ChiCTR2200063469. Registered on 7 September 2022.

Ozone assisted modification and pulverization of plant fibres

The present work investigated the effect of ozone treatment on surface modification and pulverization of plant fibres. The surface of jute, sisal, and corn husk fibres was modified by ozone treatment to remove the non-cellulosic contents and thereby improve their pulverization by ball milling process for extraction of cellulose micro/nano fibrils. The surface morphology, physical appearance, hydrophilicity, and mechanical properties of plant fibres were examined before and after the ozone treatment. Among all plant fibres, the ozone treatment of corn husk fibres was found to remove lignin and increase hydrophilicity to greater extent, however with higher tendency of defibrillation and fibre rupture. The maximum drop in tensile strength was seen in case of corn husk fibres followed by sisal and jute fibres after the ozone treatments. The tensile strength was reduced by 70%, 60%, and 47% in case of corn husk, sisal, and jute fibres respectively after 15 min of ozone treatment. When pulverization of plant fibres was carried out in ball milling, the ozone treatment was observed beneficial for easier defibrillation of cellulose micro/nano fibrils. The pulverization of jute, corn husk and sisal fibres produced the particles with diameter of 610 nm, 380 nm, and 530 nm after 15 min of ozone treatment respectively.

Effects of ozone treatment on postharvest decay of peach fruits in cold storage

Effects of ozone treatment on postharvest decay of peach fruits in cold storage

SARS-CoV-2 RNA viability on high-touch surfaces and evaluation of a continuous-flow ozonation treatment.

The COVID-19 emergency has highlighted the importance of prevention systems and environ-mental microbiological monitoring as fundamental elements in the response to epidemics and other such threats to individual and collective health. The use of automated "No-touch" room disinfection systems eliminates or reduces the dependence on operators, thus allowing an improvement in the effectiveness of terminal disinfection. In the present study, we focused on possible SARS-CoV-2 contamination of surfaces of com-mercial services, and the effectiveness of ozone treatment on the virus. Analyses were conducted on 4-7 October and 27-30 December 2021 in four supermarkets in an Apulian city; supermarkets A and B were equipped with an ozonisation system, while C and D were without any environmental remediation. SARS-CoV-2 RNA was detected by real-time RT-PCR only in December, in 6% of the surfaces tested, and all examined samples were found to be negative after viral culture, since no cytopathic effect was observed. A statistically significant difference emerged from the comparison of October vs. December (p = 0.0289), but no statistically significant difference (p = 0.6777) emerged from the comparison between supermarkets with and without the ozonisation system. Although no important changes were observed by treating the environments with ozonisation systems, further studies are needed to validate the effectiveness of environmental treatments with airborne disinfectants.