Effects Of Nutritional Factors Research Articles

Effects of Nutritional Factors on Fat Content, Fatty Acid Composition, and Sensorial Properties of Meat and Milk from Domesticated Ruminants: An Overview.

The meat and milk products from domesticated ruminants are important foods within a balanced diet, offering a rich source of energy, protein, fats, minerals, and vitamins. The sensorial properties of meat and milk are mainly linked to their fat content and fatty acid composition, which are influenced by the feeding background or nutrient composition of diets. While several studies have investigated the nutritional effects on the fat content and fatty acid profile of ruminant meat and milk, as well as their relationship with sensorial properties, a comprehensive overview of these effects is lacking. This paper summarises current literature and discusses changes to fatty acid composition (including ω-3 concentrations), fattiness, and associated quality traits of sheep, goat, beef cattle, alpaca, and llama meat that can be achieved by using different forages or feeds in a total mixed ration. Additionally, it presents the shelf life and nutritional value of meat, milk, and cheeses from the milk of dairy cattle, buffalo, goats, and sheep as influenced by a ruminant diet. Further advancement in these areas will promote the sustainability of ruminant production and its associated feeding systems in achieving premium quality animal-derived foods.

The effect of nutritional factors on urolithiasis: A case-control study.

Urolithiasis, a prevalent chronic kidney disease affecting all age groups, can be influenced by nutritional factors. The incidence of urolithiasis in Asian countries ranges from 1% to 19.1%, attributed to geographical and lifestyle differences. In Iran, several risk factors, including ethnicity, dietary habits, gender, and age, are associated with urolithiasis. This study aimed to assess the impact of nutritional factors on kidney and urinary tract stone formation. This case-control study enrolled 127 newly diagnosed urolithiasis patients, and 127 matched healthy participants between February to May 2017. Exclusion criteria included diabetes and acute or chronic renal failure. Data were collected using the Food Frequency Questionnaire (FFQ) and analyzed using chi-square and logistic regression tests. Water (95% CI: 0.09-0.89, OR=0.28), natural juices (95% CI: 0.10-0.65, OR=0.53), mineral water (95% CI: 0.05-0.64, OR=0.18), legumes (95% CI: 0.00-0.38, OR=0.032), butter, cream, or peppermint (95% CI: 0.09-0.95, OR=0.30), and ice cream (95% CI: 0.07-0.54, OR=0.203) had a significant protective effect against kidney and urinary tract stone formation. Conversely, tea consumption (95% CI: 1.15-7.99, OR=4.70), beverages (95% CI: 4.45-23.32, OR=23.32), coffee (95% CI: 1.63-11.78, OR=4.39), bread (95% CI: 1.1-10.59, OR=3.37), meat (95% CI: 1.01-8.01, OR=2.85), liver (95% CI: 3.37-488.90, OR=40.58), fish (95% CI: 2.89-216.39, OR=25.03), and various canned foods (95% CI: 1.34-10.25, OR=3.70) were significantly associated with kidney and urinary tract stone risk. These findings showed that the risk of urinary stones formation had a significant relationship with dietary habits. Therefore, the correct dietary pattern and sufficient fluid consumption may play an important role in preventing urinary stones.

Bioactive compounds, antibiotics and heavy metals: Effects on the intestinal structure and microbiome of monogastric animals – a non-systematic review

Abstract The intestinal structure and gut microbiota are essential for the animals’ health. Chemical components taken with food provide the right environment for a specific microbiome which, together with its metabolites and the products of digestion, create an environment, which in turn affects the population size of specific bacteria. Disturbances in the composition of the gut microbiota can be a reason for the malformation of guts, which has a decisive impact on the animal’s health. This review aimed to analyse scientific literature, published over the past 20 years, concerning the effect of nutritional factors on gut health, determined by the intestinal structure and microbiota of monogastric animals. Several topics have been investigated: bioactive compounds (probiotics, prebiotics, organic acids, and herbal active substances), antibiotics and heavy metals (essential minerals and toxic heavy metals).

Association between Dietary Habit and Clinical Parameters in Patients with Chronic Periodontitis Undergoing Supportive Periodontal Therapy

The recurrence risk evaluation has been emphasized in periodontal stabilization during supportive periodontal therapy (SPT). However, nutritional factors, e.g., dietary habits such as the frequency of eating vegetables, are rarely included in the evaluation. In this study, the effect of nutritional factors on clinical periodontal parameters was examined in a lifestyle-related investigation and a periodontal examination in patients with periodontitis undergoing SPT. A total of 106 patients were recruited. Tendencies toward a negative correlation were found between rate of a probing depth (PD) of 4-5 mm, rate of PD ≥ 6 mm, the bleeding on probing (BOP) rate, periodontal inflamed surface area (PISA), and various nutritional factors. The number of teeth was a clinical parameter with a significantly high R2 (≥0.10) influenced by environmental factors, whereas PD, PD of 4-5 mm, the BOP rate, and PISA were influenced by nutritional factors. These results suggested that environmental factors reflected clinical parameters showing long-term pathophysiology, such as the PD rate. Nutritional factors tended to affect the current inflammatory pathophysiology, such as the BOP rate, PISA, and PISA/periodontal epithelial surface area. Therefore, environmental and nutritional factors appear to be useful for evaluating the risk of periodontitis during SPT.

The use of therapeutic plasmapheresis in preventive and sports medicine

Maintenance of active longevity, preservation of physical activity, and prevention of decreased mobility associated with injury or age of patients are among the most urgent tasks for modern healthcare. The suppression of pathological processes and activation of defense systems at the cellular and organismal levels are the main routes for solving these problems. Several initial anti-aging therapy approaches are detoxification, rheocorrection, and immunocorrection. In these areas, methods of extracorporeal hemocorrection, in particular, therapeutic plasmapheresis, are effective. This study aimed to evaluate the effectiveness of hardware plasmapheresis with albumin compensation by assessing the dynamics of circulating age-related biomarker levels in randomly selected patients. Twenty human subjects of both sexes aged 40–55 years with an increase in one or more aging-related biomarkers participated in this study. The patients were randomly divided into two groups with ten people each. Patients from Group 1 underwent therapeutic plasmapheresis with albumin replacement (four procedures with a 2-day interval). Patients from Group 2 were offered plasmapheresis treatment with saline replacement. The levels of aging-related biomarkers were determined in the blood of patients before and 30 days after starting treatment. Preliminary data showed that plasmapheresis with albumin replacement in randomly selected male and female patients was accompanied by normalization of the selected aging biomarkers. Thirty days after the start of the plasmapheresis treatment, a decrease in both biological and phenotypic age was determined. Further studies are needed to investigate the effects of nutritional factors on aging biomarkers with and without plasmapheresis treatment. Based on the obtained results, recommendations will be made on the use of plasmapheresis in preventive and sports medicine. The use of this method will help reduce the biological age of patients and, as a result, reduce the risks of developing age-related diseases and disabilities and contribute to prolonging life and improving its quality.

Effect of nutritional factors and growth conditions on biosurfactant production by Pseudomonas mendocina and Pseudomonas oleovorans isolated from oil contaminated soil in Jeddah city

Objectives: The current study aimed to investigate the impact of different nutritional factors and growth conditions on biosurfactant production by bacterial isolates (EMB16 and EMB21) isolated from oil-contaminated soil samples. Methods: Based on the used quantitative and qualitative screening method in current study, the selected bacterial isolates showed a high potential to produce biosurfactant. Results: By using 16S rRNA sequence analysis, the bacterial isolates EMB16 and EMB21 were found to be closely related to Pseudomonas mendocina and Pseudomonas oleovorans, respectively. The ultimate yields of biosurfactant (8.06±0.06 mg/ml) by Ps. mendocina EMB16 was with corn oil as a carbon source, urea as a nitrogen source, C/N ratio of 30, pH value of 7, and 2% inoculum size. For Ps. oleovorans EMB21, the maximum biosurfactant production (4.68±0.14mg/ml) was achieved by diesel oil as a carbon source, urea as a nitrogen source, C/N ratio of 30, pH of 7, and 5% size of inoculum. The best incubation period and temperature for the examined strains was 168 hrs. at 37℃. Conclusion: The results proved that Ps. mendocina EMB16 was the most efficient biosurfactant producer as it showed the greatest amount of biosurfactant concentration and lowestvalue of surface tension measurement with an emulsification index of 67±6%.

Effect of Nutritional Factors and Copper on the Regulation of Laccase Enzyme Production in Pleurotus ostreatus.

This research aimed to establish the relationship between carbon–nitrogen nutritional factors and copper sulfate on laccase activity (LA) by Pleurotus ostreatus. Culture media composition was tested to choose the nitrogen source. Yeast extract (YE) was selected as a better nitrogen source than ammonium sulfate. Then, the effect of glucose and YE concentrations on biomass production and LA as response variables was evaluated using central composite experimental designs with and without copper. The results showed that the best culture medium composition was glucose 45 gL−1 and YE 15 gL−1, simultaneously optimizing these two response variables. The fungal transcriptome was obtained in this medium with or without copper, and the differentially expressed genes were found. The main upregulated transcripts included three laccase genes (lacc2, lacc6, and lacc10) regulated by copper, whereas the principal downregulated transcripts included a copper transporter (ctr1) and a regulator of nitrogen metabolism (nmr1). These results suggest that Ctr1, which facilitates the entry of copper into the cell, is regulated by nutrient-sufficiency conditions. Once inside, copper induces transcription of laccase genes. This finding could explain why a 10–20-fold increase in LA occurs with copper compared to cultures without copper when using the optimal concentration of YE as nitrogen sources.

Effect of nutritional factors on dental caries among preschool children in Ngha, Riyadh

Rationale: Dental caries is a multi-factorial disease which leads to the breakdown of the tooth enamel through an acid produced during bacterial fermentation of food dissolves tooth structure resulting in demineralization of teeth. Diet has an influence on the caries process. Therefore, it is important to understand different dietary choices between individuals and their impact on the development of dental caries. Aim of study to assess the nutritional factors leading to the formation of dental caries among preschool children in NGHA, Riyadh, KSA.

Green Salad Intake Is Associated with Improved Oral Cancer Survival and Lower Soluble CD44 Levels.

Deficiencies in fruit and vegetable intake have been associated with oral cancer (oral cavity and oropharyngeal). Salivary rinses contain measurable biomarkers including soluble CD44 (solCD44) and total protein, which are known markers of oral cancer risk. This study investigates the effect of nutritional factors on solCD44 and protein levels to evaluate oral cancer risk and survival. We evaluated solCD44 and protein levels from 150 patients with oral and oropharyngeal squamous cell carcinoma and 150 frequency-matched controls. We subsequently characterized the effect of food group consumption and these biomarkers on progression-free survival (PFS) and overall survival (OS). Patients reported eating fewer servings of salad (p = 0.015), while controls reported eating fewer servings of potatoes (p < 0.001). Oral cancer patients who consumed at least one serving per week of green salad were found to have significantly lower CD44 levels than those who ate salad less frequently (mean of log2[solCD44]1.73 versus 2.25, p = 0.014). Patients who consumed at least one serving per week of “salad or other vegetables” had significantly longer PFS (median 43.5 versus 9.1 months, p = 0.003, adjusted hazard ratio (HR) = 0.39 p = 0.014) and OS (median 83.6 versus 10 months, p = 0.008, adjusted HR = 0.04 p = 0.029). These findings suggest that dietary factors, namely greater green salad and vegetable intake, may be associated with lower CD44 levels and better prognosis in oral cancer patients.

Effect of nutritional factors on urinary calcium excretion in a sample of Moroccan children and adolescents

In addition to age, gender, body weight, hormonal status as well as certain physiological and pathological states, other factors exert considerable effects on calcium (Ca) retention and therefore on its urinary excretion. Among these other factors, many dietary nutrients have all been discussed as factors affecting urinary Ca through different studies. Thus, our study aims to evaluate the impact of various nutritional factors on urinary Ca excretion among a sample of Moroccan children and adolescents aged from 6 to 18 years old. A total of 133 Moroccan children and adolescents aged between 6 and 18 years were involved in this study. Participants were required from public and private schools in Rabat-Salé-Kenitra region. For each participant, anthropometry data, food record and 24-h urine samples were collected. Urinary Ca was estimated using ICP-mass spectrometry. The urinary Ca average was 73.58±37.13 mg/day. Urinary Ca excretion had no significant association (p&gt;0.05) with different studied nutrients (r=-0.082, r=0.118, r=-0.025, r=0.142, r=0.084, r=0.119 for Ca, proteins, vitamin D, sodium, phosphorus and magnesium intakes respectively).The current impact evaluation of various nutritional factors on urinary Ca excretion suggests that Ca requirement may be not influenced by nutrients intake but it is highly recommended for researchers to carry out more studies and define all factors that cause Ca deficiency in Moroccan children and adolescents.

Effect of Nutritional Factors on Growth Behaviour, Proteolytic, β-Glucosidase and β-Galactosidase Activities of Lactobacillus Cultures during Soy-Drink Fermentation

In this study, inulin, whey protein concentrate (WPC 70) and skimmed milk powder (SMP) were used as nutritional factors to improve the quality of soy-drink fermented with indigenous Lactobacillus cultures. The growth behaviour (pH, titratable acidity, viable cell count), β-glucosidase, β-galactosidase, and proteolytic activity of indigenous Lactobacillus cultures were studied with various nutritional supplements in soy-drink. Two-way analysis of variance (ANOVA) was applied and the post-hoc comparison was acquired by Tukey’s HSD test for all the data presented in the study. Due to the supplementation of nutritional factors, the pH dropped as a bit of lactic acid production was also observed due to a lack of lactose. The supplements also provided extra nutrition during their growth in different incubation hours. At 2% rate of WPC 70 supplementation, maximum proteolysis (1.17 O.D.) was found by L. fermentum K7. L. fermentum K16 showed the highest β-glucosidase enzyme activity (0.154µM/mL/min) and maximum β-galactosidase activity (0.339 µM/mL/min) was observed for L. acidipiscis K27 at 2% rate of inulin supplementation to soy-drink. In the preparation of novel fermented foods and beverages, the conjunction of nutritional supplements with indigenous Lactobacillus cultures can potentially be used.

Impaired CPT1A Gene Expression Response to Retinoic Acid Treatment in Human PBMC as Predictor of Metabolic Risk.

Ex vivo human peripheral blood mononuclear cell (PBMC) systems offer the possibility to test transcriptomic effects of food bioactive compounds with potential health effects. We investigated all-trans retinoic acid (ATRA) effect on mRNA expression of key lipid metabolism and inflammatory genes in PBMCs from normal-weight (NW) and overweight-obese (OW-OB) men with different metabolic syndrome-related features. PBMCs were incubated with 10 µM ATRA and mRNA levels of selected genes were analyzed using real-time RT-qPCR. Human ex vivo PBMCs responded to ATRA treatment, but the response for some genes was dependent on body mass index (BMI), with a lower response in PBMC from OW-OB than from NW donors. Moreover, gene expression response was affected by circulating high-density lipoprotein (HDL)-cholesterol levels. Particularly, the response to ATRA of CPT1A, previously reported as a sensitive metabolic risk predictive biomarker, was dependent on HDL levels and not on BMI, being impaired in those individuals with lower HDL levels, specifically in OW-OB. Thus, PBMCs’ insensitivity to ATRA, which can be considered as indicative of impaired metabolism, was observed in individuals with higher metabolic risk (OW-OB with low HDL levels). In conclusion, an ex vivo human PBMC system indicates that ATRA response could be influenced by metabolic syndrome features. Moreover, our study reinforces the role of CPT1A as a marker of metabolic risk and points to plasmatic HDL-cholesterol levels as a parameter to take into consideration when the effects of nutritional factors and/or dietary interventions on humans are under study. Further studies including women are required to detect potential gender differences in the observed effects.

The spatio-temporal features of chicken mitochondrial ND2 gene heteroplasmy and the effects of nutrition factors on this gene

Mitochondrial heterogeneity is the presence of two or more types of mitochondrial (mt)DNA in the same individual/tissue/cell. It is closely related to animal health and disease. ND2 is a protein-coding gene in mtDNA, which participates in mitochondrial respiratory chain and oxidative phosphorylation. In previous studies, we observed that the mt.A5703T and mt.T5727G sites in the ND2 gene were the heteroplasmic variation sites. We used pyrophosphate sequencing technology to examine chicken mt.A5703T and mt.T5727G heteroplasmic sites in the ND2 gene, in different tissues and at different development stages in chickens. We also investigated whether nutritional factors could affect the mt.A5703T and mt.T5727G heteroplasmy. Our results showed that chicken mt.A5703T and mt.T5727G heteroplasmy had clear spatio-temporal specificities, which varied between tissues/development stages. The mtDNA heterogeneity was relatively stable upon nutrition intervention, 30% dietary energy restriction (from 18 to 48 days old) and different types of dietary fats (at 5% concentration, from 1 to 42 days old) did not change the breast muscle heteroplasmy of broilers at the mt.A5703T and mt.T5727G sites. In addition, multiple potential heteroplasmic sites were detected by clone sequencing in the ND2 region, which potentially reflected abundant heteroplasmy in the chicken mitochondrial genome. These results provide an important reference for further research on heteroplasmy in chicken mitochondria.

Fizjologiczne aspekty postępowania dietetycznego w chorobie Hashimoto

Zapalenie tarczycy typu Hashimoto, nazywane popularnie chorobą Hashimoto, to przewlekłe schorzenie autoimmunologiczne charakteryzujące się podwyższonym mianem przeciwciał anty-tarczycowych we krwi i zmianami w miąższu tarczycy stwierdzanymi w badaniu ultrasonograficznym. Ponadto jest ono najczęstszą przyczyną pierwotnej niedoczynności tarczycy. Z roku na rok istotnie wzrasta liczba osób z chorobą Hashimoto i w związku z tym zainteresowanie metodami wspomagania leczenia, w tym również możliwościami dietoterapii. Niestety jak dotąd brak jest standardów postępowania dietetycznego w chorobie Hashimoto. Wiadomo natomiast, że działanie tarczycy i wydzielanych przez nią hormonów oraz mechanizmy regulujące procesy zapalne w gruczole tarczowym zależą istotnie od wielkości podaży energii oraz od ilości w diecie i składu białka i tłuszczów, a także spożycia składników mineralnych tj. jod, selen, żelazo i cynk oraz witamin D, E, A i C. Znajomość efektów i mechanizmów działania czynników żywieniowych w tym obszarze może być pomocna w formułowaniu zaleceń żywieniowych dla osób z chorobą Hashimoto.&#x0D; Słowa kluczowe: autoimmunologiczne zapalenie tarczycy typu Hashimoto, makroskładniki, mikroskładniki, oś tarczycowa

Влияние алиментарного фактора на развитие язвенного колита

The aim was to аssess the effect of nutritional factors on the risk of developing ulcerative colitis based on an analysis of food consumed. Assess the importance of consumption of certain nutrients in its development among the population of Western Siberia. Materials and methods: A survey of 81 patients with ulcerative colitis and 39 healthy volunteers was conducted. The profile of the eating habits of patients with ulcerative colitis (before diagnosis) in relation to healthy respondents was investigated. Results. The diet of patients with ulcerative colitis before the first signs of the disease is distinguished by the rare consumption of fresh and cooked vegetables and fruits in small portions, the consumption of more sugar with tea and / or coffee compared to healthy ones. Also, patients poorly tolerated milk and dairy products before the debut of the disease. Conclusion. Our study confirms the possible effect of a lack of dietary fiber and excessive sugar intake on the onset of ulcerative colitis.

Effects of nutrition factors on mortality and sepsis occurrence in a multicenter university-based surgical intensive care unit in Thailand (THAI-SICU study).

The aim of this study was to demonstrate the role of nutrition factors on a 28-d mortality outcome and sepsis occurrence in surgical intensive care unit. The data was extracted from a THAI-SICU study that prospectively recruited participants (≥18 y of age) from three Thai surgical intensive care units (SICUs) of university-based hospitals. The demographic data and nutrition factors at SICU admission included energy delivery deficit, weight loss severity, route of energy delivery, and albumin and nutrition risk screening (NRS-2002). The outcomes were 28-d hospital mortality and sepsis occurrence. The statistical analysis was performed using Cox regression. The study included 1503 eligible patients with a predominantly male population. The 28-d mortality and sepsis occurrences were 211 (14%) and 452 (30%), respectively. Regarding multivariable analysis, for mortality outcome, the protective effects of nutrition variables were higher body mass index (BMI; hazard ratio [HR], 0.82; 95% confidence interval [CI], 0.68-0.99; P = 0.039), tube feeding (HR, 0.46; 95% CI, 0.26-0.83; P = 0.010), and a combination of enteral and parenteral nutrition (HR, 0.24; 95% CI, 0.07-0.77; P = 0.016). The harmful effects were severe weight loss (HR, 1.61; 95% CI, 1.16-2.22; P = 0.004), albumin ≤2.5 (HR, 2.15; 95% CI, 1.20-3.84; P = 0.010), and at risk according to NRS-2002 (HR, 1.34; 95% CI, 0.98-1.85; P = 0.071). For the sepsis occurrence, only tube feeding had a protective effect (HR, 0.58; 95% CI, 0.39-0.88; P = 0.009), and only albumin ≤2.5 had a harmful effect (HR, 1.71; 95% CI, 1.20-2.45; P = 0.003). Nutrition factors affecting the mortality or sepsis occurrence in this study were BMI, enteral feeding or combination with parenteral nutrition, severe weight loss, preadmission albumin ≤2.5, and at risk according to NRS-2002.

The nutrition of poultry as a factor affecting litter quality and foot pad dermatitis - an updated review.

Foot pad dermatitis (FPD), a condition of inflammation and necrotic lesions on the plantar surface of the footpads, is commonly observed in fast-growing broiler chickens and turkeys. FPD negatively affects the welfare of birds, performance indices and the economic profit of poultry meat production. Nutrition is an important factor affecting water intake, excreta moisture and litter quality and, in this way, the occurrence and intensity of FPD in birds. This article reviews and discusses the recent results published in the literature on the effects of nutritional factors on litter quality and FPD severity in broiler chickens and turkeys. Literature data on the efficacy of nutritional methods on the litter quality and FPD occurrence are not consistent. However, the results of several experiments indicate that the optimal level of crude protein, biotin and electrolytes (Na, K) in the diet, as well as feed additives such as feed enzymes hydrolysating non-starch polysaccharides and organic sources of microelements (zinc), may reduce the litter moisture as well as FPD incidence and severity in broiler chickens and turkey.

REVIEW: The pig as a model for humans: Effects of nutritional factors on intestinal function and health1

REVIEW: The pig as a model for humans: Effects of nutritional factors on intestinal function and health1

Influence of Maternal Nutritional Factors on Ovarian Folliculogenesis in Cattle

Assisted reproductive technologies, such as artificial insemination and embryo transfer, have produced significant outcomes in cattle. These technologies are useful not only for improving fertility but also for increasing the rate of genetic change in beef and dairy live stock. Today, embryo transfer in cattle is both practical and commercially viable. However, bovine embryo transplantation still remains suboptimal under on-farm conditions, one of the main reasons for which may be inefficient nutritional management. The role of nutri tion in modulating reproduction and particularly in ovarian function remains unclear. Considerable effort has been made to elucidate the nutritional factors regulating the development of follicles in either a direct or an indirect manner. Moreover, evidence indicates that bovine folliculogenesis is modulated by gonadotropins as well as by metabolic hormones, including growth hormone, leptin, insulin and insulin-like growth factor-1. In addition, metabolites – such as glucose, urea and non-esterified fatty acids – are considered to play a role in regulating ovarian follicular development. Nutrition affects folliculogenesis indirectly by altering the activity of reproductive hormones. Furthermore, nutrition has local impact on the development of follicles at the ovarian level. As a result, nutrition is possibly decisive to the fate of bovine oocyte development, since oocyte quality influences reproductive outcomes such as fertilization and pregnancy rates. Although numerous reviews in the published literature describe nutritional roles in reproduction, the primary focus of this paper is the effect of nutritional factors on folliculogenesis in cattle. A greater understanding of this influence may facilitate nutritional manipulation in order to enhance fertility in cattle in a cost-effective yet very efficient way.

Nutrition for Sarcopenia.

Aging-related sarcopenia means that muscle mass, strength, and physical performance tend to decline with age, and malnutrition is associated with sarcopenia. Therefore, nutritional interventions may make an important contribution to prevent the development of sarcopenia. Here I reviewed published articles about the effects of nutritional factors on sarcopenia in elderly people. A growing body of evidence suggests that metabolic factors associated with obesity and diabetes induce the progression of sarcopenia. However, the effectiveness and safety of caloric restriction for sarcopenia remained unclear. Protein intake and physical activity are the main anabolic stimuli for muscle protein synthesis. As optimal dietary protein intake, 1.0 - 1.2 g/kg (body weight)/day with an optimal repartition over each daily meal or 25 - 30 g of high quality protein per meal were recommended to prevent sarcopenia, which was supported by some observational studies. Protein supplementation using cheese and milk protein, essential amino acids, leucine, beta-hydroxy-beta-methylbutyrate and vitamin D has been investigated as a potential supplement to improve muscle quality in sarcopenic elderly people.