Effect Of Nonessential Amino Acids Research Articles

A comparative metabolomics analysis of domestic yak (Bos grunniens) milk with human breast milk.

Yaks are tough animals living in Tibet's hypoxic stress environment. However, the metabolite composition of yak milk and its role in hypoxic stress tolerance remains largely unexplored. The similarities and differences between yak and human milk in hypoxic stress tolerance are also unclear. This study explored yak colostrum (YC) and yak mature milk (YMM) using GC-MS, and 354 metabolites were identified in yak milk. A comparative metabolomic analysis of yak and human milk metabolites showed that over 70% of metabolites were species-specific. Yak milk relies mainly on essential amino acids- arginine and essential branched-chain amino acids (BCAAs): L-isoleucine, L-leucine, and L-valine tolerate hypoxic stress. To slow hypoxic stress, human breast milk relies primarily on the neuroprotective effects of non-essential amino acids or derivates, such as citrulline, sarcosine, and creatine. In addition, metabolites related to hypoxic stress were significantly enriched in YC than in YMM. These results reveal the unique metabolite composition of yak and human milk and provide practical information for applying yak and human milk to hypoxic stress tolerance.

Effect of non-essential amino acids (proline and glutamic acid) and sugar polyol (sorbitol) on brood of honey bees

Dietary nutrients provide fuel for the growth and development of insects as well as chemicals for their tissue construction. Apis mellifera L., an important pollinator, collects nectar and pollens from different plants to get their nutritional needs. Honey bees use protein for growth and development and carbohydrates as energy sources. Pollens predominantly contain proline and glutamic acid (non-essential amino acids). This is the first study to evaluate the role of proline, glutamic acid and sorbitol on bee broods. The composition of the diet can optimize the in vitro rearing process. Therefore, we elaborated on the possible impact of these amino acids and sugar alcohol on bee broods. This study aimed to achieve this objective by rearing honey bee larvae under different concentrations of proline, glutamic acid, and sorbitol (1, 4 and 8%), which were supplemented into the standard larval diet. The supplementation of proline helped the quick development of larvae and pupae of honey bees, whereas developmental time only decreased in pupae in the case of glutamic acid. The duration of the total bee brood development was the shortest (20.1 and 20.6 days) on Pro8 and Glu4, respectively. Proline only increased larvae survival (93.8%), whereas glutamic acid did not increase the survival of any brood stage. Pupal and adult weights were also increased with proline and glutamic acid-supplemented diets. Sorbitol did not change the developmental period of the honey bee brood but increased larval survival (93.7%) only at the lowest concentration (Sor1). The small concentration of sorbitol can be used to increase the survival of the honey bee brood. However, a higher concentration (Sor8) of sorbitol reduced the body weight of both pupae and adults. This study predicted that rearing bee brood could be one of the factors for the selectivity of pollen with higher proline and glutamic acid during the foraging of bees.

Effects of Non-Essential Amino Acids on Knee Joint Conditions in Adults: A Randomised, Double-Blind, Placebo-Controlled Trial.

Joint problems impair performance during exercise and daily activities and influence quality of life. The present study aimed to examine the effects of a combination of six non-essential amino acids (6AA) on joint conditions in an adult population. A total of 50 participants aged between 20 and 64 years with joint discomfort but no diagnosed joint disorder were randomly and blindly assigned to a control or 6AA group. The 6AA group took 12 g of the non-essential amino acid formulation orally (4 g three times a day) and the control group took equivalent doses of a placebo. Each group maintained the daily dose for 12 weeks. Primary outcome measures were evaluated with the visual analogue scale (VAS), the Japanese Knee Osteoarthritis Measure (JKOM), and the Japanese Orthopaedic Association score (JOA). These tests were taken before the experiment began at 4 weeks and 12 weeks after the intervention. The results of the VAS indicated that 6AA improved joint pain, discomfort, and stiffness both during a resting state and during normal activity. Participants’ scores on the JKOM and JOA also showed significant improvements in the group that had taken the 6AA supplement. These results demonstrate that 6AA improves symptoms of joint problems, such as pain, discomfort, stiffness, and difficulty in performing daily activities after 4 weeks of daily consumption.

Amino Acids Fortification of Low-protein Diet for Broilers Under Tropical Climate. 2. Nonessential Amino Acids and Increasing Essential Amino Acids

A three-week trial was carried out to evaluate the effect of nonessential amino acids (NEAA) supplementation to a low-crude protein (CP) diet with adequate essential amino acids (EAA) level on growth performance, blood metabolites, and relative weights of abdominal fat, breast yield, and internal organs in broiler chickens raised under tropical hot and humid environment. Five isocaloric (3000 metabolisable energy/kg) corn-soybean diets were administered (1 to 21 days) to 5 groups of broilers (60 birds/group) as follows: i) 22.2% CP (positive control; PC); ii) 16.2% CP+all EAA to meet or exceed the National Research Council (1994) recommendations (negative control; NC); iii) NC+further EAA to equal the levels in the PC diet; iv) NC+NEAA to equal the levels in the PC; v) NC+EAA and NEAA to equal the amino acids levels in the PC diet. The results showed that the fortification of EAA alone, only improved feed intake (FI), whereas, addition of NEAA or EAA+NEAA significantly enhanced body weight, daily weight gain, and FI and decreased the feed conversion ratio to the same levels as in PC. Serum uric acid was significantly reduced and serum triglyceride increased in NC group. Dietary treatments had no significant effect on relative weights of heart, liver, abdominal fat, breast meat yield, serum albumin, and serum total protein. In conclusion, these results suggest that NEAA fortification may improve the growth performance of broilers fed an excessive low-CP diet under tropical hot and humid condition.

Role of nonessential amino acids on porcine embryos produced by parthenogenesis or somatic cell nuclear transfer

Amino acids play a multitude of roles during early embryonic development and have been demonstrated to facilitate improved development of in vivo or in vitro fertilized and parthenogenetic embryos in several species. However, review of emerging literatures, shows that culture milieu of cloned embryos might be different from those of in vitro fertilized embryos. This study therefore, evaluated the effect of nonessential amino acids (NEAA) on yield and quality of porcine embryos produced by somatic cell nuclear transfer (SCNT) and compared them with parthenogenetic embryos as control. Analysis showed that, supplementation of NEAA to culture medium significantly improved the blastocyst rate of parthenogenetic (38.9 +/- 8.8 vs. 27.5 +/- 9.0%) as well as SCNT (22.5 +/- 2.2 vs. 13.8 +/- 3.4%) embryos although cleavage rates were not different (P < 0.05). These blastocysts also had higher hatching ability and contained higher cell number than control blastocysts (P < 0.05). TUNEL labeling revealed that blastocysts cultured in the presence of NEAA were less predisposed to biochemical apoptosis and showed lower indices of TUNEL, fragmentation, and total apoptosis than those cultured in the absence of NEAA (P < 0.05). Real-time qRT-PCR analysis further revealed that NEAA decreased the expression ratio of BAX:BCL-xL and enhanced the relative abundance of IGF2 transcripts. Therefore, our study suggests that NEAA improves the yield and quality of cloned porcine embryos by enhancing blastocyst expansion, hatching, and total cell number and decreasing the apoptosis by positively modulating the expression of embryo survival related genes, similar to those reported for in vivo or in vitro fertilized embryos. Nonessential amino acids improve the yield and quality of cloned and parthenogenetic porcine embryos and modulate the expression of embryo survival related genes.

Effect of Essential and Nonessential Amino Acids in North Carolina State University (NCSU)-23 Medium on Development of Porcine In vitro Fertilized Embryos

The present study was conducted to examine the effect of different levels of essential and nonessential amino acid in NCSU-23 medium on the in vitro-produced porcine embryo as it develops from the zygote to the blastocyst stage. Four experiments were performed, each with a completely randomized design involving 5 to 8 replications of treatments. In order to know the effect of nonessential amino acids in NCSU-23 medium, 0, 5, 10 and 20 μl/ml MEM were supplemented there to, (Exp. 1) and the medium was supplemented with same level of essential amino acids (Exp. 2). The combined effect of nonessential (0, 5, 10 and 20 μl/ml MEM) and essential amino acids (0, 5, 10 and 10 μl/ml MEM) in NCSU-23 medium (Exp. 3), first 72 h with non-essential amino acids (at 0, 5, 10 and 20 μl/ml MEM), and last 4 d with essential amino acids with the same level as NEAA (Exp. 4) were examined. The embryo development was monitored and the quality of blastocysts was evaluated by counting the number of total cells and determining the ratio of inner cell mass (ICM) to trophoectoderm (TE) cells. When Eagle's nonessential amino acids (MEM) added to NCSU-23 medium, it significantly increased the likelihood of development to the 2- to 4-cell stage and subsequent blastocyst development. Supplementation of different levels of essential amino acids in the NCSU-23 medium decreased cleavage rate, rate of morula and blastocyst development and the number of ICMs. In the case of the combined effect of essential and nonessential amino acids, better and significant results were found for blastocysts, hatching blastocysts and for ICM numbers which were also dose dependent. With respect to the biphasic effect of nonessential and essential amino acids, nonessential amino acids increased cleavage whereas essential amino acids increased the total cell number. Neither the nonessential nor the essential group of amino acids, on their own, affected blastocyst cell number or the differentiation of cells in the blastocyst. In conclusion, this study determined the role of nonessential and essential amino acids in the culture of the porcine embryo and showed that the embryo requires different levels of amino acids as it develops from the zygote to the blastocyst stage.

Effect of nonessential amino acids on nitrogen retention in growing pigs fed on a protein-free diet supplemented with sulphur amino acids, threonine and tryptophan.

Two N balance experiments were conducted on growing pigs to study the effect of essential and nonessential amino acids added to a protein-free diet on N retention. In Expt. 1, the addition of sulphur amino acids, threonine and tryptophan to a protein-free diet at levels two times the maintenance requirements reduced (p > 0.1) daily N loss from -131 to -108 mg/kg(0.75). A further addition of nonessential amino acids equivalent to 250 mg N/kg(0.75)/d resulted in a marked increase (p < 0.01) in daily N retention to 28 mg/kg(0.75). In Expt. 2, nonessential amino acids were added to a protein-free diet supplemented with sulphur amino acids, threonine and tryptophan at levels corresponding to 100, 200 and 300 mg N/kg(0.75)/d. N retention increased linearly as dietary nonessential N increased. The slope of the best-fit regression line indicated that the marginal efficiency of nonessential N utilization for protein accretion was 0.26. The results suggest that nonessential amino acids may be a limiting factor for the re-utilization of amino acids released by body protein breakdown or that they may serve as precursors for de novo synthesis of amino acids by gut microorganisms, thus contributing to the amino acid requirements of the pig.

Effects of non-essential amino acids on long-term survival of mouse embryos cultured in vitro: a comparative study using IVF-50TM and S1-mediumTM

Effects of non-essential amino acids on long-term survival of mouse embryos cultured in vitro: a comparative study using IVF-50TM and S1-mediumTM

Influence of nonessential L-amino acids on growth of rats fed high levels of essential L-amino acids.

SummaryWeanling rats were fed purified L-amino acid diets containing high levels of essential L-amino acids (EAA) to determine the effect of nonessential amino acids (NEAA) on growth. Observations were made also on the liver composition and plasma amino nitrogen (PAN) levels. A diet containing high levels of only the EAA resulted in poor growth (3.8 g/day), as compared with a casein diet (5.6 g/day). However, when six NEAA were added to this amino acid diet, growth rate (5.2 g/day) and food efficiency were significantly improved. Proline added singly caused a significant growth improvement; on the other hand, glycine, glutamic acid, aspartic acid, alanine, serine, or urea did not significantly improve the growth rate.

Effects of Nonessential Amino Acids on the Growth of Vitamin B6-Depleted Rats

Effects of Nonessential Amino Acids on the Growth of Vitamin B6-Depleted Rats