Effect Of Nitrogen Sources Research Articles

Effect of Various Nitrogen Sources at Various Sulfur Levels on Maize–Wheat Yield and N/S Uptake under Different Climatic Conditions

Effect of Various Nitrogen Sources at Various Sulfur Levels on Maize–Wheat Yield and N/S Uptake under Different Climatic Conditions

ENHANCEMENT OF GROWTH AND YIELD QUALITY OF ONION PLANT VIA FOLIAR APPLICATION OF BIO-STIMULANTS UNDER DIFFERENT NITROGEN SOURCES

There are environmental hazards of synthetic nitrogen fertilizers, however, there is a general tendency to raise the yield and quality of strategic crops in Egypt i.e., onion plant. Also, a little is known about the joint effects of different nitrogen sources as soil addition plus foliar spraying with bio-stimulants on onion plants. Thus, this research paper aims to assess the possibility of using compost as a partial substitute for synthetic nitrogen fertilizer with onion plants sprayed with some bio stimulants. During two successive seasons (2020/2021 and 2021/2022), a field trial was performed aiming to evaluate the effect of nitrogen recommended dose (NRD) via different nitrogen sources i.e., plant compost (2.0 %N), ammonium sulphate (21.0%N), urea (46.5%N) and ammonium nitrate (33.5%N) and some bio stimulates on onion plant and some soil properties. The nitrogen treatments [N1:40% of NRD as plant compost +20% of NRD as ammonium sulphate + 20% of NRD as urea+20% of NRD as ammonium nitrate, N2:100% of NRD as ammonium sulphate, N3:100% of NRD as urea and N4:100% of NRD as ammonium nitrate] represented the main plots, while foliar application of bio-stimulants represented the sub main plots, where the foliar application treatments were F1: without (control), F2: liquorice extract (7.50 gL-1); F3: garlic extract (350.0 ml L-1) and F4: yeast extract (7.5 gL-1).The findings indicate that onion plants grown under N1 treatment had growth, yield and quality traits better than the corresponding onion plants grown under other nitrogen treatments, where N2 treatment came in the second order and N3 treatment came in the third order, while N4 treatment came in the last order. Regarding foliar application, the plants sprayed with garlic extract (F3) had the highest values of studied traits followed by that sprayed with liquorice extract (F2) then that spayed with yeast extract (F4) and lately that grown without bio stimulants (F1). Generally, the best performance of onion plants was recorded under N fertilization of 40% of NRD as plant compost + 20 % of NRD as ammonium sulphate + 20 % of NRD as urea+ 20 % of NRD as ammonium nitrate and spraying, simultaneously, with garlic extract. Also, all studied soil properties like soil EC, pH, Om and available N differed due to all studied treatments. Thus, it can be confirmed the possibility of using plant compost as a partial substitute for mineral N fertilizer with onion plants sprayed with some bio stimulants.

Effects of Different Inorganic Nitrogen Sources of Iris pseudacorus and Iris japonica on Energy Distribution, Nitrogen, and Phosphorus Removal

High- and low-affinity transport systems are the main pathways for the transportation of NO3– and NH4+ across intracellular membranes. NO3− and NH4+ are assimilated through different metabolic pathways in plants. Fifteen ATP molecules are hydrolyzed in the metabolic process of NO3–; however, only five ATP molecules are hydrolyzed in that of NH4+. In this research, seedlings of Iris pseudacorus and Iris japonica were used as the experimental materials in the NO3–:NH4+ = 30:0, NO3–:NH4+ = 28:2, NO3–:NH4+ = 27:3, NO3–:NH4+ = 15:15, NO3–:NH4+ = 3:27, and NO3–:NH4+ = 0:30 treatments at the 7.5 mmol·L−1 the total nitrogen content (TN). The intracellular free energy was represented by physiological resistance (R) and physiological impedance (Z) according to the Nernst equation and could conveniently and comprehensively determine the cellular metabolic energy (GB). The maximum absorption rate (Vmax) and Michaelis constant (Km) for NH4+ and NO3– uptake were calculated according to the kinetic equation. The results showed that the cellular metabolic energy (GB) of I. pseudacorus was 1 to 1.5 times lower than that of I. japonica at each treatment on the 10th day. The GB values of I. pseudacorus and I. japonica seedlings increased with increasing NH4+ concentration. However, there was a turning point at the NO3–:NH4+ = 15:15 treatment for the cellular metabolic energy of I. pseudacorus and I. japonica. Correlation analysis showed that the value of cellular metabolic energy was negatively correlated with the Vmax and Km for NO3– uptake, whereas it was positively correlated with that for NH4+ uptake. These results demonstrate that the NO3–:NH4+ = 27:3 treatment level was the most suitable for I. pseudacorus and I. japonica. This indicates that the greater cellular metabolic energy is the most suitable for plant growth when the concentration of ammonium or nitrate had no significant difference at treatment. These results provide a simple and rapid solution for removal of nitrogen by determination of cellular metabolic energy.

Effect of Nitrogen Source on Growth Endophytic Yeast from Salacca edulis Reinw. and Bread Quality Analysis

east biomass is often used in the fermentation of bread dough. Dough fermentation can be maximized by adding a nitrogen source. This study used yeast isolates from salak pondoh (YIS-3, YIS-4, and YIS-7). The purpose of this study was to determine the effect of nitrogen addition on yeast growth and bread quality. This study used an experimental approach. The results of the growth study showed that all isolates treated with 0.05% urea produced higher biomass and cell counts than those treated with control. The highest biomass was produced by YIS-7, which was 3.81 g/300mL, while the highest number of cells was produced by YIS-3, which was 29.02x106 cells/mL. The percentage of proofing results showed that all yeast isolates treated with 0.05% urea needed a longer time to achieve the highest proofing. However, the volume of bread after baking showed better results than those treated with control. The largest volume of bread produced by YIS-3, was 972.14 cm3. The results of the organoleptic test showed that P<5%, which means that all treatments had a significant effect on the taste, aroma, color, and texture of the bread. Overall, the panelists gave good acceptance of the bread fermented by YIS-3 with 0.05% urea treatment. So it can be concluded that the addition of urea with a concentration of 0.05% in YIS-3 gave the best effect on the yeast growth and bread quality.

Influence of carbon and nitrogen sources on structural features and immunomodulatory activity of exopolysaccharides from Ganoderma lucidum

In this work, the effects of various carbon and nitrogen sources on structural features and immunomodulatory activity of exopolysaccharides (EPS) from Ganoderma lucidum were studied. When glucose and yeast powder were used as carbon and nitrogen sources, the apparent viscosity of fermentation broth and EPS yield and the corresponding sugar content were higher than those cultured with other carbon and nitrogen sources. The main polysaccharide components (20E) with molecular weights ranging from 2.78 × 106 to 3.96 × 106 g/mol were isolated from fermentation broth through 20% ethanol precipitation. Monosaccharide analysis showed that the carbon source significantly affected the monosaccharide compositions of 20E, and the initial carbon source of the medium accounted for the largest proportion of monosaccharide in 20E. The immunoenhancing activities on inducing NF-κB activation by 20E fractions from different carbon and nitrogen combinations exhibited significant differences. Correlation analysis demonstrated that the immunomodulatory activity of 20E fractions was positively correlated with sugar contents and the molar ratios of glucose, while it was negatively correlated with the molar ratios of xylose. As the structural features and biological activities of EPS are highly dependent on the polysaccharide medium composition, this variability can be used advantageously to produce tailor-made polysaccharides with specific applications.

Spineless cactus-based diets associated with various nitrogen sources in sheep diets

This study evaluated the effects of various nitrogen (N) sources augmenting spineless cactus-based diets on intake, digestibility, rumen kinetics and N balance in sheep. Eight rumen-fistulated sheep with an average initial bodyweight of 62 ± 6.83 kg were allotted to one of two 4 x 4 Latin squares. The diets consisted of spineless cactus (Nopalea cochenillifera), Tifton hay, corn grain, vegetable oil and a mineral mixture augmented with either soybean meal (SBM), cottonseed meal (CSM), whole cottonseed (WCS) or urea (U) to provide additional nitrogen. Dry matter (DM), crude protein (CP), and neutral detergent fibre (NDF) intakes were similar (P >0.05) for sheep fed the diets with SBM, CSM, and WCS. The digestibility coefficients of DM and CP were similar (P >0.05) for SM, CM, and U diets. The rate of DM disappearance from the rumen did not differ (P >0.05) between treatments. However, the rumen ammonia, pH and N balance was higher (P <0.05) for sheep fed the WCS and U diets. The N absorbed and N retained did not differ (P >0.05) between the diets. Soybean meal and CSM are similar in their effects and can be used for sheep in diets based on spineless cactus. Whole cottonseed and U are interesting sources of N but should be used with caution owing to their effects on digestibility and nutrient intake in sheep.

Effects of Supplementary Irrigation and Nitrogen Fertilization on Characteristics of Sun Cured Tobacco in Semi-Arid Conditions

ABSTRACT Nitrogen and water should be applied at the right time and amount to obtain optimum crop yield in arid and semi-arid regions. The study was carried out in 2017–2018 to investigate the effects of different supplementary irrigation levels applied after harvests, and different nitrogen sources on water use efficiency (WUE), yield and quality characteristics of sun-cured tobacco. The study included five different nitrogen sources (N1: zero-nitrogen, N2: ammonium nitrate, N3: ammonium sulfate, N4: calcium ammonium nitrate, and N5: urea) and four different irrigation treatments (I0:No irrigation; I33:66% deficit, I66:33% deficit and I100:100% full irrigation). The effects of nitrogen sources and supplementary irrigation treatments on plant height, leaf number, leaf width, leaf height, yield, quality grade, sugars, nicotine, phenolics, WUE and irrigation water use efficiency (IWUE) were statistically significant. Seasonal crop evapotranspiration values ranged between 117.7 and 235.3 mm. The highest WUE 1.65 kg m−3 and IWUE: 10.48 kg m−3 were recorded in I33 treatment. Plant characteristics were different between nitrogen sources; however, the results did not indicate a prominent nitrogen form. The results for supplementary irrigation revealed that the I100 treatment in semi-arid conditions increased the sun-cured tobacco yield by 22% with acceptable quality improvements. The gross income increased 1705 $ ha−1 with the increase in tobacco yield and sale price per hectare.

Effect of nitrogen source on mycelial growth, blastospore production and thermotolerance of Cordyceps fumosorosea (Hypocreales: Cordycipitaceae)

Among the entomogenous fungi, Cordyceps fumosorosea has great potential for the control of piercing-sucking insects in greenhouses. The blastospores of C. fumosorosea developed in liquid fermentation are more efficient for industrialization because they can kill insects faster and be produced faster than conidia from solid cultivation. Thermotolerance of blastospores plays a major role in their application in greenhouses. The added nutrient elements during its cultivation process have a significant impact on the thermotolerance of blastospores. Understanding the effects of nitrogen sources on the mycelial growth, sporulation and thermotolerance of C. fumosorosea is essential for its commercial application. Frequently used nitrogen sources were investigated in fermentation medium, including peptone, soya peptone, yeast extract, ammonium sulfate, urea and so on. Soya peptone provided the maximum blastospore yield and GT50, which were 5.17 × 108 blastospores mL−1 and 1.91 h, respectively. To explore the difference in intracellular substances involved in the thermotolerance of blastospores in the presence of different nitrogen sources, intracellular trehalose and mannitol were extracted and then qualified by ion chromatography. The results showed that blastospores with better thermotolerance generally had lower intracellular trehalose content, indicating that the intracellular trehalose content of blastospores could be a factor that the fungus responds to thermal stress and that its content in the blastospores could be adjusted by the supplemented nitrogen source.

Effect of feeding slow release non protein nitrogen sources on milk productionand milk quality parameters in Cross bred dairy cows

A study of 90 days duration was conducted on 18 multiparous crossbred cows (363±14kg, body weight; in II or III lactation; 3,000-4,500kg milk yield per lactation) randomly allotted to 3 dietary treatments with 6 animals in each group in a completely randomized design to investigate the effect of two slow release non protein nitrogen (SRNPN) products on feed intake, milk yield, milk composition, blood biochemical parameters and cost economics. The concentrate mixture for control group was formulated with maize, gram husk, ground nut cake, wheat bran, deoiled rice bran, salt and mineral mixture. The other two experimental concentrate mixtures were prepared by totally replacing ground nut cake with calcium salts of fatty acid coated urea (CFCU) and Hydroxypropyl methylcellulose coated urea (HMCCU) at 2.42%. The cows were offered fixed quantity of Napier green fodder (10kg/cow/day) and ad libitum paddy straw. The daily allowance of respective concentrate mixture offered to each cow was calculated based on body weight, previous week milk yield and roughage intake to meet the nutrient requirements of as per ICAR (2013). Incorporation of either of the coated urea products in concentrate mixtures did not affect the total DM intake, milk yield (13.94 to 14.11kg/d), milk composition (Milk fat, Protein, SNF and Lactose) and blood biochemical (Blood urea nitrogen, Glucose and total protein). Though non significant, the average daily cost of feeding (Rs/d) was lowered in CFCU (228.14) and HMCCU (215.98) diet fed cows compared to control group cows (237.08) by 3.77 and 8.89% respectively. Whereas, the cost of feeding (Rs) per kg milk production differed significantly (P<0.01) with highest in control diet fed group (16.80) while in CFCU (15.43) and HMCCU (15.49) fed group it was lower. CFCU and HMCCU can be included at 1% of dry matter or 2.42% in the concentrate replacing the conventional protein sources, without affecting the milk yield and its composition in cross bred cows and reducing the feed cost per kg milk production.

The Effects of Nitrogen sources in the concentrates on N utilization and production performances of dairy goats

Studies in young goats showed that feeding different N supplements did not significantly affect growth rate, rumen digestion, or N metabolism. It is unclear whether this finding is also applicable to lactating dairy goats. This study aimed to evaluate the efficacy of combinations of barley meal with soybean meal, cottonseed meal, or urea in high energy concentrate on nutrient digestion, milk-feed efficiency, milk production, and Nitrogen balance in dairy goats fed on barley hay as a basal diet. Four isoenergetic and isonitrogenous concentrate with different N sources were formulated and fed on lactating Saanen goats in a 4 x 4 Latin square design. The results showed that lactating Saanen goats producing 1.3 to 1.5 litre of milk per day and fed on a concentrate containing 1.7 % N in a high energy diet capable to sustain similar milk protein and N balance as that given concentrate with 2.2 % dietary N. Total nutrient intakes, nutrient digestion, milk-feed efficiency and milk production lactating Saanen goats was not affected by the source of dietary N. The results indicate that including urea in a supplement can maintain a similar level of milk yield, milk protein and milk efficiency in goats as can the feeding of more expensive soybean and cottonseed meals provided there is sufficient readily available dietary energy.

The Effect of Medium Concentration and Nitrogen Source on the Productivity and Biochemical Composition of Arthrospira platensis

The Effect of Medium Concentration and Nitrogen Source on the Productivity and Biochemical Composition of Arthrospira platensis

Identification, Biological Characteristics of Pestalotiopsis diospyri and Its Sensitivity to Fungicides

Persimmon canker disease on stems caused by pathogens affects its growth and yield, leads to economic losses, thus it is important to investigate the pathogen in detail to find an effective control method. In this paper, samples from diseased persimmon trees in Yunnan were collected to isolate and purify the pathogen, and its biological characteristics were studied. In detail, its morphology was observed and molecular identified. The effects of different carbon and nitrogen sources, temperature, pH and light condition on its growth were analyzed, and the toxicities of 6 fungicides to the pathogen were compared. In the results, the pathogens were identified as Pestalotiopsis diospyri, the optimum conditions for its mycelium growth were on persimmon stem decoction medium, temperature 30 °C, light illumination for 24 h and pH at 4. The most suitable carbon and nitrogen sources were glucose and sodium nitrate, respectively. The sensitivity test of six fungicides to P. diospyri showed different degrees, the EC50 values of tebuconazole, azoxystrobin, chlorothalonil, difenoconazole, prochloraz, and carbendazim were 0.212, 0.313, 4.921, 0.552, 0.035 and 6.175 μg/mL, respectively, revealing that P. diospyri was the most sensitive to prochloraz. In conclusion, P. diospyri grow fastest in acidic and well-lit environments, and prochloraz can be used as a protential fungicide for the treatment and prevention of the disease. This paper provides a theoretical basis for further research and field experiment.

Effect of nitrogen and carbon sources and salt stress on the bioactive components concentration in Scenedesmus obliquus

In Scenedesmus obliquus culture, the effects of different nitrogen and carbon sources and saline stress on total carotenoids, total proteins and total phenols contents were investigated. Microalgae in Allen & Arnon culture medium were cultivated, until reaching the stationary phase. Then, sodium nitrate or urea as nitrogen source and sodium acetate or glucose as carbon source, were incorporated. Additionally, sodium chloride was added for cause saline stress. Fourteen treatments were carried out, during 40 days. Differences in bioactive components concentration were observed for the different nitrogen and carbon sources from day 26. When 0.24 g/L of urea were used, 3 times more of total carotenoids were obtained than when sodium nitrate was used; while, with 3.68 g/L of glucose, 1.5 times more of total carotenoids were reached than with sodium acetate was used. Total proteins concentration was 1.9 times higher when 0.24 g/L of urea and 5.02 g/L of sodium acetate were utilized as nitrogen and carbon sources, respectively. With 31.00 g/L of urea 4 times more of total phenols were obtained than with sodium nitrate; while, 12 times more of total phenols were achieved with 3.68 g/L of glucose than with sodium acetate. Saline stress caused a reduction in the bioactive components of interest. The highest concentrations obtained were: 513.20±13.21mg β-carotene/gdwc, 7.25±0.34 mg BSA/gdwc, and 16.78±0.54 mg GAE/gdwc. Therefore, this study confirmed that Scenedesmus obliquus microalgae developed strategies to adapt to the new conditions caused by the nitrogen and carbon sources incorporation, causing changes in the concentration of bioactive components.

Effects of nitrogen source and NaCl stress on oil production in Vischeria sp. WL1 (Eustigmatophyceae) isolated from dryland biological soil crusts in China

Effects of nitrogen source and NaCl stress on oil production in Vischeria sp. WL1 (Eustigmatophyceae) isolated from dryland biological soil crusts in China

Evaluation of nitrogen source, concentration and feeding mode for co-production of fucoxanthin and fatty acids in Phaeodactylum tricornutum

Microalgae-based wastewater treatment exhibits a competitive alternative to traditional biological techniques due to its ability to recover nutrients and produce high value-added bioproducts such as fucoxanthin and fatty acids. As an essential nutrient element, nitrogen plays a critical role in microalgal metabolism and bio–compositions synthesis including fucoxanthin and fatty acids, whose preference for nitrogen varied. However, it lacks information on whether it is feasible to achieve a suitable nitrogen-related culture condition that is preferred by the productions of both fucoxanthin and fatty acids, and simultaneously enhances microalgal growth performance and nutrient removal efficiency. This study investigated the effects of nitrogen sources, nitrogen levels and nitrogen feeding modes on microalgal biomass, nitrogen removal, and the microalgal intracellular response as represented by the co-production of fucoxanthin and fatty acids in Phaeodactylum tricornutum. With the same overall nitrogen concentration, the urea supplementation achieved over 90% nitrogen removal efficiency and compared with the other nitrogen sources including mixed ones, it also showed enhanced microalgal growth and fatty acid accumulation with the production of fucoxanthin 2.35–3.36 times higher than the other groups. Subsequently, a significantly higher accumulation of fucoxanthin (1.47 mg/g) and fatty acids (152.65 mg/g) were observed when 12.35 mg-N/L urea was added, which was the same nitrogen level included in the f/2 medium. Accordingly, with the 12.35 mg-N/L urea in total, one-time feeding on D0 and two-time feedings (fed equally on D0 and D5) were superior for the co-production of fucoxanthin and fatty acids, with the latter one also exhibiting a slightly enhanced nitrogen removal efficiency. This study systematically explored the effects of nitrogen on the co-production of fucoxanthin and fatty acid and provides a novel comparative idea for optimizing the nitrogen-related parameters for microalgal cultivation.

Antimicrobial and Antibiofilm Activities of Bacterial Strain P-6B from Segara Anakan Against MRSA 2983

Methicillin-resistant Staphylococcus aureus (MRSA) infection has become a worldwide concern due to the increasing MRSA resistance to antibiotics. This condition encourages the continuous exploration of new anti-MRSA compounds. Thus, this study explored the effect of carbon (starch, glucose, sucrose, lactose) and nitrogen (casein, yeast extract, urea, peptone) sources for the fermentation medium to produce antibacterial and antibiofilm compounds from bacterial strain P-6B against MRSA 2983. The results showed that six-day incubation produced bioactive extracts with the highest antimicrobial activity (11.361 ± 0.860 mm) and the highest activity to inhibit biofilm formation (89.159 ± 0.293% %). In comparison, three-day incubation produced a bioactive extract with the highest activity to degrade biofilm (86.450 ± 3.260%). Glucose was the best carbon source to produce an extract with antibacterial and biofilm inhibition activities. However, starch was the best carbon source to produce an extract with biofilm degradation activity. Urea was the best nitrogen source to produce extracts with antimicrobial and antibiofilm activities. Species identification based on the 16S rRNA gene revealed a 99.78% similarity with Ochrobactrum intermedium strain NBRC 15820 (NR_113812.1). These findings suggested the potency of Ochrobactrum P-6B from Segara Anakan Cilacap as indigenous sources of bioactive compounds with anti-MRSA activities.

The growth and yield responses of two bean cultivars to organic and inorganic nitrogen sources

Abstract. Pangaribuan DH, Ermawati, Suryani NMW, Maria WS. 2022. The growth and yield responses of two bean cultivars to organic and inorganic nitrogen sources. Biodiversitas 23: 1677-1682.The productivity of beans is increased by selecting the best cultivar and applying fertilizers according to the needs of the plant. In addition,the application of high-yielding cultivar and N fertilizers with the right dosage could be used to increase the yield. Therefore, the objective of thisstudy is to determine the effect of nitrogensources on the growth and yieldof two bean cultivars. A5×2 factorial treatment was usedin a randomized block design with three replications. The first factor is the Perkasa and Lebat-3 cultivar, the second is organic and inorganic nitrogen fertilizer, namely control, urea 100kgha-1, as well as 10, 20, and 30% concentrationsof liquid organic fertilizer from leucaena leaves. The results showed that the use of liquid organic fertilizers derived from leucaena could be used as an alternative to nitrogenfertilization. Furthermore,the 20% concentrationtreatment producedthe highest number of trifoliate leaves (8.81 leaves) and dry weight (4.52 g). Based on the results,the use of 20% liquid organic fertilizer withPerkasa cultivar showed the best effect on the number of pods per plant (13.38),and yield(846.67 kg ha-1). The technology of liquid organic fertilizers from leucaena leaves could be recommended for bean organic production.

Macrolactin Metabolite Production by Bacillus sp. ZJ318 Isolated from Marine Sediment.

A total of 172 microbial strains were screened and isolated from Arctic Ocean marine sediments at a depth of 42 ~ 3,763m. A microorganism with strong antibacterial activity against Staphylococcus aureus was identified as Bacillus sp. ZJ318 according to the results of 16S rDNA sequencing and phylogenetic tree analyses. Bioactivity-guided isolation of the new/novel metabolite in the ethyl acetate (EA) extract obtained from the fermentation broth of this strain was followed by chromatographic fractionation and subsequent HPLC purification, leading to the isolation of one known macrolactin. The chemical structure of the macrolactin, which indicated macrolactin J isolation from marine microorganisms for the first time, was assigned based on a high-resolution electrospray ionization mass spectrometer system (HR-EMI-MS), nuclear magnetic resonance (NMR) spectral analyses, and a literature review. To improve macrolactin J production, the corresponding effects of nitrogen sources were investigated, and (NH4)2SO4 was determined to produce the best effect. In addition, the optimal culture conditions were determined by an orthogonal experiment. Under these conditions, the yield of macrolactin J was increased to 2.41mg/L, which was 2.2 times the original yield. This work lays a foundation for follow-up mechanistic and application research on macrolactin J.

Evaluating the effect of nitrogen fertilizer rate and source on the performance of open‐pollinated rye (Secale cereale L.) cultivars in contrasting European environments

AbstractRye (Secale cereale L.) is a minor cereal with potential to support sustainable farming practices. This study evaluated the performance of four old and modern European open‐pollinated winter rye cultivars grown with different nitrogen (N) sources (cattle slurry, farmyard manure, biogas digestate, mineral N) at two rates of N 50 and 100 kg ha−1 in the United Kingdom and Estonia, in the 2014–2015 and 2015–2016 growing seasons. Grain yield in Estonia was higher than the United Kingdom (6.2 vs. 4.8 t ha−1), which was attributed to a higher ear density (409.4 vs. 249.7 ears m−2) and grain number per ear (50.2 vs. 43.2), although 1,000‐grain weight was higher in the United Kingdom. There were clear differences between sites in the response to N source with significant N source × year interactions. Biogas digestate and mineral N produced higher grain yield than farmyard manure and cattle slurry in the United Kingdom, whereas in Estonia cattle slurry had a lower grain yield than the other N sources. Foliar diseases were absent in Estonia and in the United Kingdom, and N source only affected powdery mildew and leaf blotch on Leaf 2. The response of grain quality to N source and variety was consistent across both sites. The modern variety Elias had the highest Hagberg falling number and specific weight but the lowest protein content, whereas the variety Schlägler had the highest protein content but lowest specific weight. The benefits of selecting cultivars for specific environments are shown by the interactions between genotype and environment.

Effect of Nitrogen Sources on Photosynthesis and Biosynthesis of Alkaloids and Leaf Volatile Compounds in Annona sylvatica A. St.-Hil

Effect of Nitrogen Sources on Photosynthesis and Biosynthesis of Alkaloids and Leaf Volatile Compounds in Annona sylvatica A. St.-Hil