Effect Of Methylthiouracil Research Articles

Ameliorative effect of methylthiouracil on TGFBIp-induced septic responses

The screening of bioactive compound libraries can be an effective approach for repositioning FDA-approved drugs or discovering new treatments for human diseases. Transforming growth factor β-induced protein (TGFBIp) is an extracellular matrix protein whose expression in several cell types is greatly increased by TGF-β. TGFBIp is released by human umbilical vein endothelial cells (HUVECs), and functions as a mediator of experimental sepsis. Here, we investigated the anti-septic effects and underlying mechanisms of methylthiouracil (MTU), used as antithyroid drug, against TGFBIp-mediated septic responses in HUVECs and mice. The anti-inflammatory activities of MTU were determined by measuring permeability, human neutrophils adhesion and migration, and activation of pro-inflammatory proteins in TGFBIp-activated HUVECs and mice. According to the results, MTU effectively inhibited lipopolysaccharide-induced release of TGFBIp, and suppressed TGFBIp-mediated septic responses, such as hyperpermeability, adhesion and migration of leukocytes, and expression of cell adhesion molecules. In addition, MTU suppressed CLP-induced sepsis lethality and pulmonary injury. Collectively, these results indicate that MTU could be a potential therapeutic agent for treatment of various severe vascular inflammatory diseases via inhibition of the TGFBIp signaling pathway.

Effects of methylthiouracil on the proliferation and apoptosis of rat bone marrow stromal cells.

The aim of the present study was to investigate the effects of methylthiouracil (MTU) on the proliferation and apoptosis of rat bone marrow stromal cells (BMSCs). Rat BMSCs were isolated, cultured in vitro and treated with various concentrations of MTU. Cell growth curves were determined using the Cell Counting Kit-8 method and the effect of MTU on BMSCs in a logarithmic growth phase was observed. BMSC apoptosis following MTU treatment was detected by flow cytometry. The experimental results demonstrated that the proliferation-inhibition effect was gradually enhanced with increasing MTU concentrations and the extension of treatment time. Statistically significant differences were observed between the treatment and the control groups (P<0.05). In addition, the BMSC apoptosis rate gradually increased with increasing drug concentrations and treatment time extension; statistically significant differences were observed between the treatment and the control groups (P<0.05). Therefore, the results of the present study demonstrated that MTU inhibited the proliferation of BMSCs and promoted apoptosis, indicating the cytotoxic effects of MTU on BMSCs.

Effects of propylthiouracil and methylthiouracil on cyclic AMP and ion movements in rat pancreatic islets.

Propylthiouracil and methylthiouracil have been shown to potentiate glucose-induced insulin secretion from rat pancreatic islets: the effect of methylthiouracil being less pronounced than that of propylthiouracil. In this study the effects of these substances on cAMP levels, 86Rb+ efflux, 45Ca2+ net uptake, and 45Ca2+ efflux were tested in isolated rat islets in order to obtain information on their possible mechanism of action. Propylthiouracil and to a lesser extent methylthiouracil increased islet cyclic AMP in a concentration-related manner. Maximum increases at the highest concentrations tested were 261% and 190% respectively. In the presence of 3 mM glucose propylthiouracil and methylthiouracil led to a decrease in the 86Rb efflux rate. With 5.6 mM glucose, both thiourea derivatives produced an increase in the 86Rb+ efflux rate which was independent of the presence or absence of calcium in the medium. Propylthiouracil and methylthiouracil augmented the 45Ca2+ efflux rate in the presence as well as in the absence of external calcium at various glucose concentrations. Propylthiouracil did not change, and methylthiouracil only slightly augmented, 45Ca2+ net uptake into the isolated islets. It is suggested that the synergistic effect of propylthiouracil and methylthiouracil on glucose-induced insulin release is at least in part due to an increase in islet cAMP levels. Whether the two substances have additional direct effects on ionic fluxes which contribute to their insulinotropic action or whether the observed changes in ion movements are secondary to the elevation of cAMP levels remains to be unclear and needs further investigation.

Effects of methylthiouracil on the differentiation of rat submandibular gland.

Effects of methylthiouracil on the differentiation of rat submandibular gland.

Effect of methylthiouracil and triiodothyronine on development of the central nervous system in chicken embryos

Hypothyroidism in chick embryos was produced experimentally by injecting methylthiouracil (MTU) into eggs on Day 10 of incubation. It resulted in retarded development of structures in the cerebellar cortex and cerebrum of the embryos. Other manifestations were seen in the smaller size of the embryos, the presence of a large yolk sac even on the 21st day of incubation, and delay in hatching. Retardation was also evidenced by broadening of the external granular layer (EGL), narrowing of the molecular layer (ML), a decrease in the number of cells in the internal granular layer (IGL), and in a reduction in the size of Purkinje cells and their nuclei. In the cerebrum, the cells were more densely packed and cellular and nuclear sizes were reduced. The indices of labelled cells and mitotic divisions were higher in the EGL and in the cerebrum of MTU-treated embryos; this indicates a prolongation of the proliferative phase. The cerebrum showed slight reaction to the enzymes studied in the MTU-treated embryos. The Purkinje cells showed strong reaction to succinic dehydrogenase and NADH and NADPH diaphorases. The ML and IGL also reacted but less markedly. Embryos treated with MTU + triiodothyronine (T 3) or saline did not differ from the normal controls. T 3 injection was also ineffective.

Effects of Methylthiouracil on Prolactin Levels and Tail Height inTriturus Cristatus Carnifex

Abstract L'ipofisi di femmine di Tritone crestato allevate per 120 giorni in una soluzionc al 2‰ di metiltiouracile, coltivate in vitro per tre ore, rilasciano nel brodo di coltura quantita significativamente minori di prolattina rispetto alle ipofisi di animali di controllo. Negli animali trattati con il tioureico l'altezza della pinna caudale, un carattere ambisessuale prolattina dipendentc, e rimasta significativamente minore rispetto ai controlli per tutta la durata dell'esperienza. Questi dati indicano che nel Tritone crestato il tioureico esercita un effetto inibitore sulla increzione, e probabilmente anche sulla sintesi di prolattina.

Effects of methylthiouracil or thyroidectomy on activation of pituitary acid phosphatases in vitro by whole hypothalmic extract.

Effects of Methylthiouracil or Thyroidectomy on Activation of Pituitary Acid Phosphatases in vitro by Whole Hypothalamic Extract

THE EFFECT OF GOITROGENS ON THE HYPOTHALAMIC NEUROSECRETION IN EXPERIMENTAL ANIMALS

The weight of the thyroidal gland increased by the use of methylthiouracil and was inhibited by chlorpromazine in experimental animals. The administration of MTU provoked not only an enlargement of the thyroid gland, but also an elevation of neurosecretion of the hypothalamic neurosecretory system. Thyroidectomy produced the most remarkable changes of the hypothalamic neurosecretory system. Chlorpromazine inhibited the hypothalamic neurosecretion and depressed the release of pituitary thyrotrophin. Thus the effect of methylthiouracil, chlorpromazine, and potassium iodide on the thyroid gland appears to be mediated through the hypothalamic neurosecretory system.

Studies on the Effect of Methyl-Thiouracil upon the Development of Experimental Liver Cancer by Ortho-Amido-Azotoluene (O-A-T)

Studies on the Effect of Methyl-Thiouracil upon the Development of Experimental Liver Cancer by Ortho-Amido-Azotoluene (O-A-T)

Thyroid and ovary; the effect of methylthiouracil on ovarian function

An noch nicht geschlechtsreifen Albinoratten wurde der Einflus von 4-Methyl-2-thiouracil auf den „Hohlweg-Effekt“ untersucht. Bei Herabsetzung der Schilddrusentatigkeit durch 4-Methyl-2-thiouracil war die Zahl der Tiere, die nach alleiniger Zufuhr von Ostradiol mit Corpus luteum-Bildung im Ovarium reagierten, eindeutig vermindert.

Effect of Methyl Thiouracil and Thiouracil in Swine

Effect of Methyl Thiouracil and Thiouracil in Swine

Effects of Methyl Thiouracil in the Rations of Lambs and Aged Ewes

Effects of Methyl Thiouracil in the Rations of Lambs and Aged Ewes