Abstract
The aim of the present study was to investigate the effects of methylthiouracil (MTU) on the proliferation and apoptosis of rat bone marrow stromal cells (BMSCs). Rat BMSCs were isolated, cultured in vitro and treated with various concentrations of MTU. Cell growth curves were determined using the Cell Counting Kit-8 method and the effect of MTU on BMSCs in a logarithmic growth phase was observed. BMSC apoptosis following MTU treatment was detected by flow cytometry. The experimental results demonstrated that the proliferation-inhibition effect was gradually enhanced with increasing MTU concentrations and the extension of treatment time. Statistically significant differences were observed between the treatment and the control groups (P<0.05). In addition, the BMSC apoptosis rate gradually increased with increasing drug concentrations and treatment time extension; statistically significant differences were observed between the treatment and the control groups (P<0.05). Therefore, the results of the present study demonstrated that MTU inhibited the proliferation of BMSCs and promoted apoptosis, indicating the cytotoxic effects of MTU on BMSCs.
Highlights
Hematopoietic stem cells (HSCs) are an important index that reflect the function and status of hematopoiesis in the bone marrow, the growth of which requires a number of stimulation factors [1,2]
The method is based on various morphological observational studies on Bone marrow stromal cells (BMSCs) under an inverted phase contrast microscope [14,15]
In the present study, when the BMSCs adherently grew, the suspended HSCs and other cells were gradually eliminated with the solution replacement
Summary
Hematopoietic stem cells (HSCs) are an important index that reflect the function and status of hematopoiesis in the bone marrow, the growth of which requires a number of stimulation factors [1,2]. It has been reported that >10 types of cell growth factors exist with various stimulation effects. The secretion, release and function of cell growth factors depend on the hematopoietic microenvironment, which is important for HSC growth and development. Bone marrow stromal cells (BMSCs) are the main components of the hematopoietic microenvironment. Studying the effects of BMSCs on hematopoiesis is important for investigating the hematopoietic function and microenvironment status. BMSCs constitute the main backbone of the bone marrow microenvironment (BMM) and perform a variety of functions involved with extracellular matrix secretion, cell surface adhesion, cytokine solubility and membrane connectivity. A lack of BMSCs may result in the apoptosis of partially immature B lymphocytes [10]
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