Effect Of Low Temperature Storage Research Articles

The Effect of Low Temperature Storage on the Lipid Quality of Fish, Either Alone or Combined with Alternative Preservation Technologies.

Marine foods are highly perishable products due to their high content of polyunsaturated fatty acids, which can be readily oxidized to form peroxides and secondary oxidation products, thus conferring such foods undesirable organoleptic characteristics and generating harmful compounds that are detrimental to the health of consumers. The use of preservation methods that minimize lipid oxidation is required in the fishing and aquaculture industries. Low temperature storage (chilling or freezing) is one of the most commonly used preservation methods for fish and seafood, although it has been shown that the oxidation of the lipid fraction of such products is partially but not completely inhibited at low temperatures. The extent of lipid oxidation depends on the species and the storage temperature and time, among other factors. This paper reviews the effect of low temperature storage on the lipid quality of fish, either alone or in combination with other preservation techniques. The use of antioxidant additives, high hydrostatic pressure, irradiation, ozonation, ultrasounds, pulsed electric fields, and the design of novel packaging can help preserve chilled or frozen fish products, although further research is needed to develop more efficient fish preservation processes from an economic, nutritional, sensory, and sustainable standpoint.

Effects of low-temperature storage on the aroma composition and quality of Golden Empress Hami melons: A transcriptomic and metabolomic analysis

Low-temperature storage is frequently employed to prolong the shelf life of Hami melons. Nevertheless, extended low-temperature storage can hamper the synthesis of aroma compounds in these fruits. This study investigated the effects of storage at 3 °C (LT) and 21 °C (RT) on the aroma quality of Golden Empress Hami melons (GEHMs) during a shelf life of 24 d. Further, the transcription levels of genes related to aroma metabolism were also examined during this period. After 12 d, the intensity of sweet aroma (LT: 2.3, RT: 8.2) and fruit aroma (LT: 2.1, RT: 8) was significantly lower in GEHMs stored at LT than in those stored at RT. Correspondingly, between-group differences in the content of esters with sweet and fruity aromas (RT: 782.76 ± 128.41 μg/kg, LT: 53.69 ± 24.32 μg/kg) and alcohols (RT: 2252.98 ± 297.65 μg/kg, LT: 448.80 ± 106.13 μg/kg) peaked at 12 d. Furthermore, the expression levels of 52 genes related to aroma volatile biosynthesis were significantly different between GEHMs stored at RT and LT. Specifically, the expression levels of genes encoding ethanol dehydrogenase (ADH), long-chain acyl-CoA synthetase (ACSL), and branched-chain amino acid aminotransferase (ilvE) which are involved in the production of alcohols and esters— were significantly lower in GEHMs stored at LT than in those stored at RT. This study provides a deeper understanding of aroma-related biosynthetic pathways of GEHMs during postharvest storage. Additionally, it offers valuable metabolomic and transcriptomic resources for the regulation of aroma compound metabolism in postharvest GEHMs.

Metabolomic and transcriptomic integrated analysis revealed the decrease of monoterpenes accumulation in table grapes during long time low temperature storage

Low temperature is the commonly used technique for maintaining the quality of table grapes during postharvest storage. However, this technique could strongly affect the aromatic flavor of fruit. Monoterpenes are the key compounds contributing to the Muscat aromas of grapes. The detailed information and molecular mechanisms underlying the changes in monoterpenes during postharvest low temperature storage have not been thoroughly characterized. In this study, the effects of low temperature storage on the free and bound monoterpene profiles in four cultivars of table grape were determined at both the transcriptomic and metabolomic levels. A total of 27 compounds in both free and bound forms were identified in the four cultivars and showed quantitative differences between the cultivars. Hierarchical cluster and principal component analysis indicated that the free and bound monoterpene profiles were remarkably affected by the low temperature storage. The monoterpenes in the same biosynthesis pathway were clustered together and showed similar evolution trends during low temperature storage. And the content of most of free monoterpenes underwent a rapid decline during low-temperature storage at a certain stage, but the time was different in 4 grape cultivars. Transcriptomic analysis revealed that the expression of DXS, HDR, GPPS and TPS genes involved in the monoterpene synthesis pathway were consistent with the changes in the accumulation of monoterpene compounds. While the expression of HMGS, HMGR genes in MVA pathway and branch genes GGPPS and FPPS were negatively correlated with the accumulation of monoterpenes. The findings provide new insights into the underlying mechanisms of the berry aroma flavor change during low temperature storage.

Effect of low temperature storage of trichocards on laboratory performance of Trichogramma japonicum Ashmead

Effect of low temperature storage of trichocards on laboratory performance of Trichogramma japonicum Ashmead

Effect of cold storage on some biological and physiological performance of Adalia decempunctata L.

Adalia decempunctata (L.) is a common predator in agro ecosystems. Here, the effects of low-temperature storage on biological parameters, energy reserves, and antioxidant enzymes were investigated. Eggs, larvae, and pupae of this predator were maintained for 1-4weeks and adults for different days (20, 35, 50, and 65 d) at 4, 7, and 10°C in darkness and 70±5% R.H. After storage of 20days, the survival rate in male and female insects was >90% in 4, 7, and 10±1°C. In 65days, the highest survival rate for adult insects was recorded at 4°C. Effect of cold storage on the performance of adult's A. decempunctata was not statistically different for periods up to 35days, but decreased longer duration (65days). The female adults were able to tolerate the cold storage period by producing higher amounts of glucose and antioxidant enzymes, thus displaying a high survival rate. Based on our results, maintaining A. decempunctata adults at low temperatures (i.e. 4, 7, and 10°C) for 20 or 35days performed best without loss of quality.

Shotgun proteomics of Nanicão and Prata bananas reveals changes that may account for their different resistance to low temperature

Low-temperature storage has been widely used to extend the post-harvest life of many fruits. However, tolerance to cold is largely variable even among different fruit cultivars. In this regard, the banana cultivars Nanicão and Prata display different responses to low-temperature storage. Prata is less affected by cold storage, while the synthesis of volatiles is drastically reduced in Nanicão bananas. Therefore, this study aimed to investigate the effect of low temperature storage at the proteome level using a shotgun proteomics approach to identify a large number of proteins in these banana varieties. Seventy-four differentially expressed proteins were identified in cv. Prata and 43 in cv. Nanicão. Principal component analysis indicated that samples from control and cold storage groups could be differentiated based on their proteome. Overall, low temperatures decreased proteins related to carbohydrate metabolism and energy, defense, and transport in Nanicão bananas. Contrastingly, low temperatures induced the accumulation of proteins for carbohydrate metabolism and energy, transport, and defense in Prata bananas. Therefore, the observed changes in the proteome during cold acclimation and ripening in Nanicão and Prata bananas can explain, at least in part, the differences in low-temperature tolerance between these cultivars.

Impact of Low-Temperature Storage on the Microstructure, Digestibility, and Absorption Capacity of Cooked Rice.

This study examined the effects of low-temperature storage on the microstructural, absorptive, and digestive properties of cooked rice. Cooked rice was refrigerated and stored at 4 °C for 0.5, 1, 3, 5, and 7 days, as well as frozen and preserved at −20, −40, and −80 °C for 0.5, 1, 3, 5, 7, 14, 21, and 28 days. The results indicated that the stored rice samples generally exhibited a higher absorption capacity for oil, cholesterol, and glucose than the freshly cooked rice. In addition, after storage, the digestibility of the cooked rice declined, namely, the rapidly digestible starch (RDS) content and estimated glycemic index (eGI) decreased, whereas the slowly digestible starch (SDS) and resistant starch (RS) content increased. Moreover, the increment of the storage temperatures or the extension of storage periods led to a lower amylolysis efficiency. Scanning electron microscopy (SEM) analysis indicated that storage temperature and duration could effectively modify the micromorphology of the stored rice samples and their digestion. Moreover, microstructural differences after storage and during simulated intestinal digestion could be correlated to the variations in the absorption capacity and digestibility. The findings from this study will be useful in providing alternative storage procedures to prepare rice products with improved nutritional qualities and functional properties.

Effect of Low Temperature Storage In Sea Urchin Eggs Viability

Effect of Low Temperature Storage In Sea Urchin Eggs Viability

Effects of Chilling Injury, Physical and Biochemical Changes on Grafted Watermelons Stored at Low Temperature

Watermelon fruit from Crimson Tide (CT) and Crisby (CR) grafted onto Ferro, RS841, Argentario, and Macis rootstocks and ungrafted CT and CR were compared for effects of low temperature storage on chilling injury, physical and biochemical changes at 0°C and 85‒90% relative humidity for 21 days. After storage, fruit were hold to 21°C and 75‒80% relative humidity for 7 days to determine shelf life. Quality analyses were determined during storage and shelf life at a weekly interval. The chilling injury areas covered

Significantly Different Lipid Profile Analysis of Litopenaeus vannamei under Low-Temperature Storage by UPLC-Q-Exactive Orbitrap/MS.

Low-temperature storage is one of the most important preservation methods for aquatic product storage. However, the effects of low-temperature storage on the lipid profiles of shrimp are unclear. Herein, UPLC-Q-Exactive Orbitrap/MS combined with LipidSearch software was applied to analyze the effect of three low storage temperatures (4 °C, −2 °C, and −18 °C) on the lipidomics of Litopenaeus vannamei. A total of 15 lipid classes were analyzed, and PC, PE, DG, and TG accounted for vast majority of peak areas. Furthermore, 531 individual lipid variables enriched in 12 metabolic pathways were identified via bioinformatics analysis methods. A total of 56 significantly different lipid molecular species (55 belonging to PC, PE, DG, and TG) were selected as potential biomarkers of lipid oxidation via correlational analysis between physical properties (texture and color) and individual lipid variables. The results indicated that the three low storage temperatures caused different effects on the lipidomics profile of L. vannamei, and PC, PE, DG, and TG could become potential focuses in further studies of lipid oxidation in L. vannamei.

Efficacy of extracts from cryopreserved placenta on third-degree burns in rats

Human placenta extracts have anti-inflammatory, antioxidant and wound-healing properties, so they are promising drugs for the treatment of wounds of various origins, including burns. Cryopreservation methods are widely used to preserve the biological activity of placental drugs for a long time. The aim of this work was to study the effect of low-temperature storage of the placenta on the regenerative properties of its extracts. Fragments of freshly obtained placentas were cooled by immersion in liquid nitrogen and stored at –196 °C for 6 months. The placenta was warmed in a water bath at 37 °C. The effect of low-temperature preservation of the placenta on the ability of its extracts to positively affect the wound healing process was studied in a model of thermal burn of III B degree in rats. The effectiveness of wound treatment with extracts from cryopreserved placenta was evaluated by planimetric and histological methods at 3, 7, 14, 21 and 28 days after the burn. The activity of antioxidant enzymes in the serum of animals was also determined. superoxide dismutase activity was assessed by inhibition of adrenaline autooxidation in carbonate buffer, catalase activity was assessed by the degree of inhibition of ammonium peroxide formation. It has been shown that the treatment of burns with extracts from cryopreserved placenta helped to accelerate the regeneration processes and the rate of wound healing. The formation of granulation tissue was detected on the 7th day of treatment with extracts, and on the 14th day in the control. The area of burn wounds during treatment with extracts probably differed from the control starting from 14 days after application of the burn. It was found that the dynamics of recovery of catalase activity after burns is probably higher on the 7th day of treatment with extracts. The obtained data testify to the high efficiency of application of placenta stored at low-temperature for the purpose of obtaining extracts from it with preservation of regenerative properties.

Different bio-chemical changes at low temperature storage on chilling injury and storage life of commercial cultivars of mango Mangifera indica L.

An experiment was conducted at Fruit Research Station, Sangareddy, Medak, Telangana to study the effect of low temperature storage on chilling injury and storage life of different commercial cultivars of mango (Mangifera indica L.) viz., Banganpalli, Dashehari, Peddarasam, Chinnarasam and Totapari stored at 8oC ± 1º C. The experimental design followed is completely randomized design with factorial concept with four replications per treatment.Various biochemical parameters like TSS, titratable acidity, ascorbic acid, reducing sugars, non reducing sugars and electrolyte leakage. The highest T.S.S. (15.35) was recorded in mango cv. Peddarasam, Among all interactions, the highest TSS (16.75) was recorded on 55th day with the mango cv. Peddarasam. The lowest acidity (0.70) was recorded in mango cv. Chinnarasam and the highest acidity (1.00) was recorded in cv. Totapari. The lowest brix acid ratio (10.04) was noticed in mango cv. Totapari. The highest (23.53) was recorded in mango cv. Peddarasam. The treated fruits were significantly influenced by low temperature with lowest reducing sugars (2.11) recorded in mango cv. Totapari. The highest total sugars (6.51) were recorded in mango cv. Peddarasam. The lowest total sugars (4.40) were recorded in mango cv. Totapari. The treated fruits differed significantly with highest vitamin-C (17.07) was recorded in mango cv Peddarasam while the lowest vitamin-C (9.67) recorded in mango cv. Banganpalli. The treated fruits differed significantly with lowest electrolyte leakage (13.85) recorded in mango cv. Totapari.

Effects of Low Temperature Storage on Energy Metabolism，Related Physiology and Quality in‘Jinhong’Apple Fruit

Effects of Low Temperature Storage on Energy Metabolism，Related Physiology and Quality in‘Jinhong’Apple Fruit

Effects of Low Temperature Storage on the Expression of Genes Involved in Maturation and Softening of Kiwifruit Fruits

Effects of Low Temperature Storage on the Expression of Genes Involved in Maturation and Softening of Kiwifruit Fruits

Optimisation of Mycobacterium bovis BCG Fermentation and Storage Survival.

Mycobacterium bovis Bacillus Calmette–Guérin (M. bovis BCG) was generated over a century ago for protection against Mycobacterium tuberculosis (Mtb) and is one the oldest vaccines still in use. The BCG vaccine is currently produced using a pellicle growth method, which is a complex and lengthy process that has been challenging to standardise. Fermentation for BCG vaccine production would reduce the complexity associated with pellicle growth and increase batch to batch reproducibility. This more standardised growth lends itself to quantification of the total number of bacilli in the BCG vaccine by alternative approaches, such as flow cytometry, which can also provide information about the metabolic status of the bacterial population. The aim of the work reported here was to determine which batch fermentation conditions and storage conditions give the most favourable outcomes in terms of the yield and stability of live M. bovis BCG Danish bacilli. We compared different media and assessed growth over time in culture, using total viable counts, total bacterial counts, and turbidity throughout culture. We applied fluorescent viability dyes and flow cytometry to measure real-time within-culture viability. Culture samples were stored in different cryoprotectants at different temperatures to assess the effect of these combined conditions on bacterial titres. Roisin’s minimal medium and Middlebrook 7H9 medium gave comparable, high titres in fermenters. Flow cytometry proved to be a useful tool for enumeration of total bacterial counts and in the assessment of within-culture cell viability and cell death. Of the cryoprotectants evaluated, 5% (v/v) DMSO showed the most significant positive effect on survival and reduced the negative effects of low temperature storage on M. bovis BCG Danish viability. In conclusion, we have shown a reproducible, more standardised approach for the production, evaluation, and storage of high titre, viable, BCG vaccine.

The effects of low-temperature storage on the viability of Lactobacillus casei and the stability of antibacterial activity in green grass jelly synbiotic drinks

Abstract. Rizal S, Suharyono, Nurainy F, Amela JR. 2020. The effects of low-temperature storage on the viability of Lactobacillus casei and the stability of antibacterial activity in green grass jelly synbiotic drinks. Biodiversitas 21: 3826-3831. Synbiotic drinks from green grass jelly have shown antibacterial activity against pathogenic bacteria. These are usually stored at low temperatures to maintain their characteristics. The aim of this study was to determine the effect of storage at low temperature of 10°C on the viability of lactic acid bacteria (Lactobacillus casei) and the stability of the antibacterial activity in synbiotic drinks made of green grass jelly. Antibacterial activity of green grass jelly synbiotic drink was conducted against pathogenic bacteria (Staphylococcus aureus, Salmonella sp., Bacillus cereus, and Escherichia coli). The products were stored for 28 days at 10°C temperature. Observations on the antibacterial activity, pH value, total acid, and total lactic acid bacteria were carried out every 7 days. Antibacterial activity was evaluated using agar well diffusion method. The results showed that storage at low temperature (10 ± 2°C) for 28 days decreased the antibacterial activity and pH value but sharply increased total lactic acid bacteria (at 0 to 7 days of storage) in green grass jelly synbiotic drinks. Salmonella sp. showed the highest inhibition caused by the antibacterial agents in green grass jelly synbiotic drinks while the lowest inhibition was found on Staphylococcus aureus. During storage at low temperature, green grass jelly synbiotic drinks had a total of lactic acid bacteria that ranged from 9.51 to 10.10 (Log CFU/mL) or equal to 3.24x109-1.26x1010 CFU/mL; a total of lactic acid that ranged from 0.48% to 0.87%; and pH values that ranged from 3.78 to 4.08.

Effects of low temperature storage of Mastura (J37) jackfruit bulbs on the physical quality of jackfruit frozen confection

Mastura (J37) jackfruit variety planted in Pahang (Malaysia) is less preferred by the consumers due to its low sweetness and high-water content properties. This has caused major backlog in the plantation as reported by Pahang State Farmers Association (PASFA). In this study, among the proposed solutions given was to build a frozen confection processing line to further process the flesh. The jackfruit used were vacuum-packed and stored under refrigerated and frozen conditions. The overrun, melting resistance, and hardness of jackfruit frozen confection produced from jackfruit stored in both low temperature conditions showed comparable results. The overrun of 50 to 55 % were obtained for frozen samples at different weeks. Jackfruit frozen confections had lower overrun compared to the controlled sample as air incorporation was prevented by the elements contained in jackfruit. The control frozen confection sample without addition of jackfruit puree resulted in an average melting mass of 24.6 g and melting resistance of 59 % which was the strongest melting resistance in comparison to other jackfruit frozen confections. The inconsistent hardness of jackfruit frozen confections suggests that the content of total soluble solid increased throughout week 1 to week 3 for refrigeration storage and week 1 to week 6 for deep-freeze storage had no trending effect on the hardness of frozen confection. The output obtained from this work provides data for the downstream processing of Mastura (J37) jackfruit. These data are helpful as they contribute towards the understanding of further processing of this particular jackfruit variety into end products, in order to solve the issue faced by PASFA. The solution helps decrease waste generated from the surplus and value add the variety.

Omics analysis of the ethylene signal transduction in tomato as a function of storage temperature

This study has focussed on the effect of low temperature storage on the postharvest ripening of green tomatoes; more specifically, on the ethylene signal transduction in relation to the ethylene biosynthesis pathway. To this end, the first elements of the ethylene signal transduction, ethylene receptors, CTR and EIN2 have been analysed in tomatoes stored at three different temperatures in terms of both their gene and protein expression levels. These results have been related to quality changes and enzymes and intermediates of the ethylene biosynthesis pathway to better understand the ethylene control of ripening under low temperatures. The first changes observed during low temperature storage took place at the ethylene biosynthesis level, which might enhance changes in the ethylene signal transduction. Although expression of most ethylene signalling genes decreased with chilling, none of the protein levels showed a significant decrease, pointing at a differential regulation at the gene and protein level.

Effects of cold storage on quality of Chrysopa pallens and recovery of fecundity by insulin

The green lacewing, Chrysopa pallens Wesmael, is one of the most beneficial and prolific insects found in many horticultural and agricultural cropping system. Here, the effects of low temperature storage on quality of C. pallens were investigated by storing cocoons at 10 °C for different days. Results revealed, after removal from cold storage, emergence rate declined gradually as storage duration increased. After storage of 20 days, the emergence rate in cold-stored group is about 62.8% of that in unstored group. After eclosion, lifetime fecundity, preemergence period, oviposition period and longevity of adults in cold-stored group showed curves similar to emergence rate. However, preoviposition period and egg hatchability were not significantly affected by cold. After being stored for 20 days, the total fecundity of females emerging from cold-stored cocoons was about 64.5% of that of females emerging from unstored cocoons. Six days post emergence, females in cold-stored group showed apparent arrest of ovarian development and significant reductions of protease, lipase and trehalase activities when compared to unstored controls. When bovine insulin was exogenously used, the females emerging from cold-stored cocoons dramatically restored ovarian development and reproductive capacity. These results suggested that C. pallens pupae are suitable for cold storage and insulin hormone can be used as reproduction stimuli in this predatory species after cold storage.

Влияние низкотемпературного хранения плаценты на антиоксидантное действие ее экстрактов по отношению к эритроцитам в состоянии окислительного стресса, вызванного нитритом натрия

Влияние низкотемпературного хранения плаценты на антиоксидантное действие ее экстрактов по отношению к эритроцитам в состоянии окислительного стресса, вызванного нитритом натрия