Abstract
Human placenta extracts have anti-inflammatory, antioxidant and wound-healing properties, so they are promising drugs for the treatment of wounds of various origins, including burns. Cryopreservation methods are widely used to preserve the biological activity of placental drugs for a long time. The aim of this work was to study the effect of low-temperature storage of the placenta on the regenerative properties of its extracts. Fragments of freshly obtained placentas were cooled by immersion in liquid nitrogen and stored at –196 °C for 6 months. The placenta was warmed in a water bath at 37 °C. The effect of low-temperature preservation of the placenta on the ability of its extracts to positively affect the wound healing process was studied in a model of thermal burn of III B degree in rats. The effectiveness of wound treatment with extracts from cryopreserved placenta was evaluated by planimetric and histological methods at 3, 7, 14, 21 and 28 days after the burn. The activity of antioxidant enzymes in the serum of animals was also determined. superoxide dismutase activity was assessed by inhibition of adrenaline autooxidation in carbonate buffer, catalase activity was assessed by the degree of inhibition of ammonium peroxide formation. It has been shown that the treatment of burns with extracts from cryopreserved placenta helped to accelerate the regeneration processes and the rate of wound healing. The formation of granulation tissue was detected on the 7th day of treatment with extracts, and on the 14th day in the control. The area of burn wounds during treatment with extracts probably differed from the control starting from 14 days after application of the burn. It was found that the dynamics of recovery of catalase activity after burns is probably higher on the 7th day of treatment with extracts. The obtained data testify to the high efficiency of application of placenta stored at low-temperature for the purpose of obtaining extracts from it with preservation of regenerative properties.
Highlights
The use of low temperatures, on the one hand, promotes more efficient release of biologically active substances into the extraction solution, and on the other it ensures the preservation of high viability of various cryopreservation objects, including cells and tissues of fetoplacental origin (Ananian et al, 2019; Nikulina et al, 2019; Prokopyuk et al, 2020)
Severe burn injury caused necrosis of all layers of the injured skin area with the attachment of adjacent tissues, which led to the formation of burns of III B degree
In the area of damage from deep thermal burns on the first day, the wound healing process is in the first phase (Stone et al, 2018), which is accompanied by blood stasis in the capillaries and ischemia, resulting in weakened tissue respiration in cells of this area with subsequent death of epithelial cells of demarcation zone (Bhatia et al, 2016)
Summary
The use of low temperatures, on the one hand, promotes more efficient release of biologically active substances into the extraction solution, and on the other it ensures the preservation of high viability of various cryopreservation objects, including cells and tissues of fetoplacental origin (Ananian et al, 2019; Nikulina et al, 2019; Prokopyuk et al, 2020). Central to such objects is the placenta itself, because as a non-classical but highly active endocrine gland, it purposefully influences the development and formation of all human organs and systems before birth. There is a large number of examples of successful use of cryopreserved placental extracts in gynecology, namely in the correction of urogenital disorders in women, in the immunization of pregnant women, in the treatment of diabetes during pregnancy, as well as in the treatment of chronic fetal hypoxia (Grischenko & Yurchenko, 2011)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
