Effect Of Low-temperature Plasma Research Articles

Effects of low-temperature plasma, microwave and 60Co γ-irradiation treatments on the flavor characteristics of lychee whole fruit powder

Powdering whole lychee fruit is one of the most effective methods for improving the comprehensive utilization rate of lychees. In this study, lychee whole fruit powder (LWFP) was prepared and treated with low-temperature plasma, microwave, and 60Co γ-irradiation treatments to enhance its quality. Volatile organic compounds (VOCs) were analyzed using Headspace Solid Phase Micro-extraction Gas Chromatography-mass Spectrometry (HS-SPME-GC-MS), Headspace Gas Chromatography Ion Mobility Spectrometry (HS-GC-IMS), and electronic nose (E-nose) techniques. Electronic tongue (E-tongue) analysis revealed that both sweetness and bitterness were significant taste indicators of LWFP. GC-MS analysis revealed that the predominant volatile compounds in LWFP were terpenoids and alcohols, specifically nerol, geraniol, ɑ-pinene, hexylbenzene, ɑ-caryophyllene, caryophyllene oxide, and isoamylenol. GC-IMS identified small-molecule aldehydes such as 2-methyl propanal, hexanal, propanal, and 2-methylbutanal as significant contributors to the aroma profile. Moreover, key aromatic contributors include dimethyl sulfide, 1-penten-3-one, and acetic acid. Notably, microwave treatment exerted the most significant effect on the aroma profile of lychee. In contrast, low-temperature plasma and irradiation treatments exhibited similar and consistent changes. Specifically, microwave treatment primarily increased the concentration of larger terpenoid molecules such as nerol, 1-phenylhexane, and caryophyllene oxide, whereas low-temperature plasma and irradiation had a greater impact on smaller aldehyde molecules such as hexanal and 2-methylbutanal. All treatments enriched the sweet fruity, brilliant woody, and cabbage scents of LWFP.

Effect of Low-Temperature Plasma on Polyketone Films: Changes in the Chemical Structure and Surface Morphology

Effect of Low-Temperature Plasma on Polyketone Films: Changes in the Chemical Structure and Surface Morphology

Effect of low current cold atmospheric plasma on grains surface structure and water absorption capacity

The use of preparatory electrophysical methods of influencing food raw materials is one of the main trends in the development of innovative processes and technologies in the food and processing industry. Based on the physical effect of electron emission from a thermal emission source, a cold atmospheric plasma (CAP) was obtained, which was successfully applied to the grain material. Physical characteristics and evolution of low-temperature atmospheric plasma were considered as the main methods of analysis of electrophysical effects. To assess the effect of low-temperature plasma on grain material, measurements of water absorption capacity and analysis of surface modification by electron scanning microscopy were carried out. It has been experimentally established that CAP treatment contributes to a more intensive process of water absorption due to changes in the surface structure of the grain material. The total duration of the process of water absorption of grain material after processing of CAP decreased by more than three times until the equilibrium moisture content was reached. Scanning electron microscopy has shown that the processing of CAP leads to the appearance of a fine-mesh structure of the surface of the grain material. The effect of CAP treatment leads to modification of the seed surface, which consists in the manifestation of a fine-meshed structure on the surface of the seeds. Taking into account the advantages of CAP technology, namely the absence of the need for vacuuming and short processing time, the technology has a high practical potential.

Cold Non-equilibrium Plasma as a Tool for Air Disinfection and Destruction of Biological Objects

Introduction: The paper presents the results of experiments on the purification of air con-taminated with S. aureus, as well as the destruction of yeast under the action of cold nonequilibrium plasma of a nanosecond streamer corona discharge. Methods: It is shown that plasma is an effective means for destroying both bacteria in the air and biological objects on the surface. The main parameters of the nanosecond streamer corona discharge and their relationship with the operating time of the active plasma components were determined. It was experimentally established that the inactivation time of 80% of S. aureus bacteria with a con-centration of 2.1∙104 KOE m-3 was 0.4 s with a specific energy of 28 J∙l-1. Results: The results of measurements of the dependence of the yeast destruction degree on the yeast size, the time of direct exposure to the nanosecond streamer corona discharge, active elements of cold nonequilibrium plasma, and third-party ultraviolet radiation are presented. Conclusion: A comparison of the results of air disinfection experiments with data on yeast destruc-tion shows that the latter can be used for rapid analysis of the effects of low-temperature plasma on other biological objects.

Effect of Low-Temperature Plasma on the Structure of Surface Layers and Gas-Separation Properties of Poly(Vinyltrimethylsilane) Membranes

Effect of Low-Temperature Plasma on the Structure of Surface Layers and Gas-Separation Properties of Poly(Vinyltrimethylsilane) Membranes

THE INFLUENCE OF LOW-TEMPERATURE PLASMA ON PERMANENCE OF ANTIMICROBIAL NANO-FINISH

This paper describes the effect of low-temperature plasma on increasing permanence of surface finish of textile materials using an antimicrobial nanosol. Selected textile materials (polyester and polyamide woven fabrics, polypropylene non-woven fabric) were pre-treated by surface activation with low-temperature plasma at atmospheric pressure and subsequently finished using an antimicrobial (AMB) nanosol with a concentration of 60 ppm Ag+, 120 ppm Ag+ in the application solution. The goal was to increase the permanence of AMB nano-coating of textiles after washing and drying. To verify the effect of low-temperature plasma on increasing the permanence of the nanolayer, washing and drying was performed in accordance with the STN EN ISO 6330 standard. To determine antibacterial activity and effectiveness of the nano-coated textile materials, a quantitative test method was used in accordance with the technical standard AATCC TM 100. Evaluation of the antibacterial activity of the textile materials was performed before washing and after 20 washing cycles.

Influence of low-temperature plasma on green algae culture

Influence of low-temperature plasma, which was formed in a high-resource arc-discharge plasma jet in an argon-air mixture, was studied for a community of green algae as Chlorella vulgaris Beyerinck [Beijerinck] and Stichococcus bacillaris Nägeli. It was found that the plasma treatment for 10 minutes led to partial cell death. At the same time, the species of C. vulgaris were less sensitive to the plasma treatment than the species of S. bacillaris. After plasma exposure, the predominant growth of the latter culture was observed in comparison with the control samples. This effect can be explained by an activation of biochemical processes in the algae due to the interaction with radicals in the low-temperature plasma. The results obtained indicate the selectivity of the low-temperature plasma effect on green algae community.

No-ozone cold plasma can kill oral pathogenic microbes in H2O2-dependent and independent manner

To apply the sterilisation effect of low-temperature plasma to the oral cavity, the issue of ozone from plasma must be addressed. In this study, a new technology for generating cold plasma with almost no ozone is developed and is named Nozone (no-ozone) Cold Plasma (NCP) technology. The antimicrobial efficacy of the NCP against four oral pathogens is tested, and its specific mechanism is elucidated. The treatment of NCP on oral pathogenic microbes on a solid medium generated a growth inhibition zone. When NCP is applied to oral pathogens in a liquid medium, the growth of microbes decreased by more than 105 colony forming units, and the bactericidal effect of NCP remained after the installation of dental tips. The bactericidal effect of NCP in the liquid medium is due to the increase in hydrogen peroxide levels in the medium. However, the bactericidal effect of NCP in the solid medium depends on the charged elements of the NCP. Furthermore, the surface bactericidal efficiency of the dental-tip-installed NCP is proportional to the pore size of the tips and inversely proportional to the length of the tips. Overall, we expect this NCP device to be widely used in dentistry in the near future.

Effects for barley growth and development of single exposure to low-temperature argon plasma at different organogenesis stages

The paper presents the vegetation experiments results on the low-temperature argon plasma effect on barley plants ( Hordeum vulgare L.) of the Vladimir cultivar and its influence on morphophysiological parameters and yield. Plasma treatment was once at three organogenesis stages of barley plants: 3rd leaf, tillering and booting. Plasma exposure was 15 and 30 min. The barley plants were grown to full maturity. Analysis of barley yield structure did not reveal clear patterns in the change in most parameters resulted from the plasma treatment. However, 15 min plasma exposure on barley plants in the critical development stage (3rd leaf) increased by 77.8 % (p 0.05) the root weight of plants compared with control. After treatment at the tillering stage, the number of spikelets per main stem ear increased by 18.5 % (p 0.001) after 15 min plasma exposure, and by 11.17 % (p 0.05) after 30 min exposure. An increase in the number of productive stems and the number of grains per lateral stem ear was observed. At the same time, 30 min exposure in the 3rd leaf stage reduced by 7 % (p 0.05) the plant height. And the treatment in the tillering stage reduced by 39 % (p 0.01) the root weight of barley plants. The effect of low-temperature plasma on barley plants at the booting stage was less expressed to the plasma effect at earlier development stages. This can be explained by the lower sensitivity of this stage of organogenesis. The obtained effects of single exposure to low-temperature argon plasma at different organogenesis stages of barley plants can be useful to increase barley yields.

Effect of low-temperature plasma on Phytophthora spp

Effect of low-temperature plasma on Phytophthora spp

Study on the effect of low-temperature atmospheric pressure plasma jet on the morphofunctional properties of living tissues (in vivo)

The results of spectral-fluorescent and diffuse-optical studies of living tissues under the effect of low-temperature atmospheric pressure plasma jet in a mixture of air and argon are presented. Potential fluorophores are identified and coefficients of optical absorption and transport scattering of tissues are determined. It was revealed plasma jet irradiation of tissue leads to an increase in the intensity of fluorescence and the diffuse reflection coefficient, which is linked with a twofold increase in the transport scattering coefficient and a change in the concentration of endogenous chromophores --- a reducing water content, lipids and bilirubin to 20%, and an increasing levels of oxygen saturation to 10%. On the basis of the analysis, it is shown that the therapeutic effect of low-temperature plasma can be associated with increased antioxidant protection and the development of compensatory dehydration processes. Keywords: biological tissues, cold plasma, probing, spectroscopy, fluorescence, diffuse reflection, optical coefficients, light scattering, biochemistry, morphology.

Исследование морфофункциональных свойств биотканей ( in vivo) при воздействии низкотемпературной плазменной струи атмосферного давления

The results of spectral-fluorescent and diffuse-optical studies of living tissues under the effect of low-temperature atmospheric pressure plasma jet in a mixture of air and argon are presented. Potential fluorophores are identified and coefficients of optical absorption and transport scattering of tissues are determined. It was revealed plasma jet irradiation of tissue leads to an increase in the intensity of fluorescence and the diffuse reflection coefficient, which is linked with a twofold increase in the transport scattering coefficient and a change in the concentration of endogenous chromophores – a reducing water content, lipids and bilirubin to 20%, and an increasing levels of oxygen saturation to 10%. On the basis of the analysis, it is shown that the therapeutic effect of low-temperature plasma can be associated with increased antioxidant protection and the development of compensatory dehydration processes.

Protective effects of low-temperature plasma on cisplatin-induced nephrotoxicity

The application of atmospheric pressure low-temperature plasma (LTP) in medical treatment has received extensive attention owing to its redox regulatory and anti-inflammatory properties. Nephrotoxicity due to oxidative stress and inflammation is the main adverse effect of cisplatin. In the present study, rats with cisplatin-induced nephrotoxicity were treated with LTP to investigate its potential protective effect. The results showed that LTP treatment has multiple protective effects on cisplatin-induced nephrotoxicity. It significantly improved clinical indicators such as survival rate, water intake, food intake, body weight, and mobility, as well as physiological indexes such as reduced renal index and levels of serum urea, creatinine, and total bilirubin; pathological indicators such as reduced tubular injury, inflammatory infiltration, tubulointerstitial fibrosis, and apoptosis; cell survival indicators such as decreased protein levels of Caspase-3 and Bax and increased Bcl-2; anti-oxidation status such as reduced malondialdehyde content and increased activities of catalase, superoxide dismutase, and glutathione peroxidase; and reduced inflammatory factors such as TNF-α in kidney tissues. Specially, LTP treatment did not influence the anticancer effect of cisplatin as observed in the solid tumor mouse model established by subcutaneously inoculating H22 cells. Moreover, LTP did not influence the physiological and pathological indicators of normal rats, suggesting its biological safety. In conclusion, LTP can protect against cisplatin-induced nephrotoxicity through its anti-oxidation, anti-inflammation, and anti-apoptosis effects, without influencing the anticancer effect of cisplatin.

Atmospheric Pressure Plasma Irradiation Facilitates Transdermal Permeability of Aniline Blue on Porcine Skin and the Cellular Permeability of Keratinocytes with the Production of Nitric Oxide

The transdermal delivery system of nutrients, cosmetics, and drugs is particularly attractive for painless, noninvasive delivery and sustainable release. Recently, atmospheric pressure plasma techniques have been of great interest to improve the drug absorption rate in transdermal delivery. Currently, plasma-mediated changes in the lipid composition of the stratum corneum are considered a possible mechanism to increase transdermal permeability. Nevertheless, its molecular and cellular mechanisms in transdermal delivery have been largely confined and still veiled. Herein, we present the effects of cold plasma on transdermal transmission on porcine skin and the cellular permeability of keratinocytes and further demonstrate the production of nitric oxide from keratinocytes. Consequently, argon plasma irradiation for 60 s resulted in 2.5-fold higher transdermal absorption of aniline blue dye on porcine skin compared to the nontreated control. In addition, the plasma-treated keratinocytes showed an increased transmission of high-molecular-weight molecules (70 and 150 kDa) with the production of nitric oxide. Therefore, these findings suggest a promoting effect of low-temperature plasma on transdermal absorption, even for high-molecular-weight molecules. Moreover, plasma-induced nitric oxide from keratinocytes is likely to regulate transdermal permeability in the epidermal layer.

Experience of low-temperature plasma radiofrequency treatment of 53 patients with tongue hemangioma

BackgroundThe main treatments of hemangioma of the tongueare are injection of sclerotherapy, freezing, surgical resection or combined application of the above methods. It is extremely difficult to remove it completely. Even if combined with multiple methods, it is often unable to control its continued growth, and many important physiological functions of the tongue will be affected, resulting in poor quality of life for patients. ObjectiveTo probe into the therapeutic effect of low-temperature plasma on hemangioma of the tongue. MethodsThe clinical data of 53 patients with hemangioma of the tongue admitted to our department from July 2013 to July 2019 were retrospectively analyzed and studied. ResultsLow-temperature plasma radiofrequency to treat hemangioma of the tongue might have advantages of simple operation, wide vision, fewer postoperative complications and quick recovery. SignificanceThe low-temperature plasma system possesses advantages of precise location, light collateral damage, non‑carbonization of the wound surface, light postoperative response, fewer complications and faster recovery. Combined with the endoscopic system with different angles, it can fully expose the surgical field and is especially suitable for the surgical treatment of patients with tumor of the tongue, which is worth popularizing application in clinic.

Application of cold plasma to control the microbiota composition on the surface of potato tubers

A study of the effect of low-temperature plasma on potato tubers was carried out. A comparative assessment of changes in the rate of germination, the size of shoots and the mass of shoots was carried out. Changes in the number of bacteria and fungi on the surface of tubers were analysed for different durations of exposure. It was found that growth characteristics did not change. The number of bacteria on the surface of tubers was significantly reduced due to the exposure to low-temperature plasma.

The Role of Mitochondria in the Dual Effect of Low-Temperature Plasma on Human Bone Marrow Stem Cells: From Apoptosis to Activation of Cell Proliferation

The potential use of low-temperature plasma (LTP) for therapeutic purposes has aroused the concern of many researchers. This paper examines the effect of LTP on the morphofunctional state of human bone marrow stem cells (BMSC). It has been established that LTP-induced oxidative stress has a dual effect on the state of stem cells. On the one hand, a cell culture exposed to LTP exhibits the progression of a destructive processes, which is manifested by the perturbation of the cell’s morphology, the initiation of lipid peroxidation and the accumulation of products of this process, like diene conjugates and malondialdehyde, as well as the induction of mitochondrial dysfunction, leading to cell death. On the other hand, the effect of LTP on BMSC located at a distance from the plasma is accompanied by the activation of proliferative processes, as evidenced by the tendency of the activity of mitochondrial biogenesis and fission/fusion processes to increase. The paper discusses the role of mitochondria and reactive oxygen species (ROS) in the cellular response to LTP.

Effect of Low Temperature Plasma Treatment on Biological Characteristics and Yield Components of Wheat Seeds (Triticum aestivum L.)

The impacts of a low temperature plasma treatment on wheat seed germination and field growth were investigated using a plasma system about the first generation (T0), the second generation (T1) and the third generation (T2). Untreated wheat seeds were set as the control (CK). Plasma treatments of 80 W and 100 W were employed during the experiments. Germination characteristics, seedling growth parameters, biological characters and yield components were measured following the plasma treatments. The wheat seed germination index, plant height, tiller, growth and grain number obviously changed significantly after the low-temperature plasma treatment. Furthermore, the moderate-intensity plasma had an active impact on wheat seed germination and growth. The results showed that the theoretical yield of T0 significantly increased by 8.92% and 8.14%, while the actual yield increased by 8.12% and 6.10% compared with untreated. The theoretical yield of T1 increased by 5.69% and 6.62%, while it increased by 3.70% and 0.45% for T2. The actual yield of T1 increased by 5.75% and 4.94%, while it increased by 3.81% and 0.82% for T2. Plasma treatment exhibited the following trend of increasing: T0 > T1 > T2 > CK. We conclude that the effects of low-temperature plasma on the biological characteristics and yield components of wheat seeds exhibited a consistent trend from T0 to T1 to T2.

Cold atmospheric pressure plasma decontamination of allspice berries and effect on qualitative characteristics

The effect of low-temperature plasma generated at atmospheric pressure was studied in term of reduction of bacterial contamination and influence on nutritional characteristics of allspice (Pimenta dioica). The low-temperature plasma was generated in ambient air using Diffuse Coplanar Surface Barrier Discharge (DCSBD). The bacterial species inoculated on the surface of samples were reduced in dependence on exposure time. Plasma treatment time of 240 s led to log10 CFU/g of Salmonella Enteritidis to below detection level (1.0 log10 CFU/g) from initial population of 7.64 log10 CFU/g. After treatment with DCSBD plasma for 300 s, the populations of Bacillus subtilis and Bacillus subtilis endospores were decreased by 2.85 log10 CFU/g and 1.70 log10 CFU/g, respectively. The parameters related to the decontamination effect were measured. Temperature of samples does not exceed the value declared as low-temperature limit and water activity decreased after plasma treatment. The effect of plasma on the qualitative properties of allspice studied by infrared spectroscopy showed no changes in characteristic chemical bonds on the surface and also inside the samples. Measurements of polyphenolics content in allspice showed that plasma has negligible impact on these main constituents affecting taste and flavor. The experiments demonstrated an efficient reduction in microbial contamination and minimal influence on the aroma profile of treated and stored spice.

Оценка возможностей улучшения обрабатываемости изделий инструментом из быстрорежущей стали с модифицированной рабочей частью

There are presented results of the comparative operation of two high-speed steel drill types: common ones and those with the working part modified by low-temperature plasma effect. A possibility to improve product machinability according to the criterion of productivity at the expense of the durability period increase of modified drills is shown. 
 There are formulated requirements to the results of low-temperature plasma modification ensuring tool life increase.