The purpose of this research is to study the effect of heat generation on mixed convection flow on Nano fluids over a horizontal circular cylinder of a heated in two dimension form. A stream of fluids are steady and incompressible, a stream flowing vertically upwards for circular cylinder and the boundary layer at the stagnation point. Three different types of nanoparticles considered are Cu, Al2O3, and TiO2. Mixed convection flow in Nano fluids on the surface of a circular cylinder will cause the boundary layer. The governing boundary layer equations are transformed into a non-dimensional form, and then the non-dimensional forms are transformed into a similar boundary equations by using stream function. Furthermore, an implicit finite-difference scheme known as the Keller-box method is applied to solve numerically the resulting similar boundary layer equations. The result of the research by varying the non-dimensional parameters are mixed convection, Prandtl number, nanoparticle volume fraction, heat generation, and radius of a cylinder are as follows. First, the velocity profile increase and temperature profile decrease when mixed convection parameter increase. Second, the velocity and temperature profiles decrease when Prandtl number parameter increase. Third, the velocity profile with the variation of nanoparticle volume fraction (χ) is increased when the value of χ is 0,1 ≤ χ ≤ 0,15 and the velocity profile decreases when the value of χ is 0,19 ≤ χ ≤ 0,5 while the temperature profile is increasing when the value of χ is 0,1 ≤ χ ≤ 0,5. Fourth, the velocity and temperature profiles increase when heat generation and the radius of the cylinder increase. The last, Cu, Al2O3, and TiO2 nanoparticles produce the same velocity and temperature profiles, but the three types of nanoparticles are different at the velocity and temperature values.