A study was conducted during 2016-2017 and 2017-2018 seasons at the Experimental Farm, Faculty of Agriculture, South Valley University, Qena, Egypt, with the objective to study the effect of different date of sowing and fertilization treatments on productivity of barley cultivar Giza 121. The experiment was in a split-plot arrangement was based on a randomized complete block design (RCBD) with three replications. Three sowing dates were assigned to the main plots and thirteen fertilization treatments to the sub-plots. There was a significant effect of the interaction between sowing dates and fertilization treatments on study traits (plant height, spike length, spike weight, number of spikes/m2, 1000-grain weight, grain yield/plant, grain yield per feddan and straw yield per feddan). The highest values of previous traits were recorded under sowing barley cultivar Giza 121 at 15th of November and applied of 75% recommended NPK + biofertilization + humic acid. Keywords: Sowing date, NPK, humic acid, biofertilizer