Effect Of DIDS Research Articles

Inhibitory effects of 4,4′-diisothiocyanostilbene-2,2′-disulfonate (DIDS) on the ADP-stimulated aggregation of gel-filtered bovine blood platelets

The effect of DIDS, a specific inhibitor of anion transport in the erythrocyte membrane, on the ADP-stimulated aggregation of gel-filtered bovine blood platelets was examined. Marked inhibition of aggregation was observed at concentrations of more than 5 x 10(-5)M DIDS. On preincubation with platelets for 30 min, DIDS was more potent and significant inhibition was observed at concentrations of over 2 x 10(-7)M. Since ADP-stimulated aggregation of bovine gel-filtered platelets precedes the release reaction, these results suggest that an anion transport system in the plasma membrane is involved in platelet aggregation.

Bicarbonate uptake by basolateral membrane vesicles from rat jejunum.

Basolateral membranes from rat jejunal enterocytes have been obtained by self-orienting Percoll-gradient centrifugation. Bicarbonate and L-glucose uptake into osmotically active basolateral membrane vesicles has been studied by a rapid filtration technique. In closed vessels and at pH 8.2 the uptake kinetics of both [14C]bicarbonate and L[3H]glucose have been followed for 30 min at 18 degrees C. Bicarbonate uptake seems to be fast and in efflux experiments SITS and DIDS effect is negligible. This work demonstrates that it is possible to determine bicarbonate flux across basolateral membrane vesicles at pH and temperature values close to usual experimental conditions.

Specific drug sensitive transport pathways for chloride and potassium ions in steady-state ehrlich mouse ascites tumor cells

A major aim of this investigation was to determine whether, in steady-state ascites cells, Cl − transport can be partitioned into a furosemide-sensitive cotransport with K + and a separate 4,4′-isothiocyanostilbene-2,2′-disulfonic acid (DIDS) sensitive self-exchange. Both Cl − and K + fluxes were studied. The furosemide- and Cl − sensitive K + fluxes were equivalent, both in normal ionic media and when the external K + concentration, [K +] o, was varied from 4 to 30 mM. The stoichiometry of the furosemide-sensitive Cl − and K + fluxes was 2 Cl −: 1 K + at 0.1 and 0.5 mM drug levels but increased to 3 Cl − : 1 K + at 1.0 mM furosemide. DIDS at 0.1 mM had no effect on the K + exchange rate but inhibited Cl − exchange by 39% ± 2 (S.E.). The effects of DIDS and 0.5 mM furosemide on Cl − transport were additive but 1.0 mM furosemide and DIDS had overlapping inhibitory actions. Thus furosemide acts on components of K + and Cl − transport which are linked to each other, but the drug also inhibits an additional DIDS-sensitive Cl − pathway, when present at higher concentrations. The dependence of the furosemide-sensitive K + and Cl − transport on [K +] o was also studied; both fluxes fell as the [K +] o increased. The latter results recall those in an earlier study by Hempling (Hempling, H.G. (1962) J. Cell. Comp. Physiol. 60, 181–198).