Effect Of Collagen Hydrolysate Research Articles

Effects of collagen hydrolysate on the stability of anthocyanins: Degradation kinetics, conformational change and interactional characteristics

Anthocyanins are desirable compounds in the food industry owing to their attractive color and high biological activity; however, their poor stability remains a substantial challenge. Here, we show that low-concentration (15 mg/mL) collagen hydrolysate (CH) exhibits a potent stabilization effect on red cabbage anthocyanins (RCAs). CH extended the half-life of RCA by 6.2-fold from 40.7 to 251.1 h. Dynamic light scattering and transmission electron microscopy confirmed the formation of CH–RCA complexes, which exhibited stronger antioxidant activity than RCA alone. Ultraviolet-vis and infrared spectra demonstrated that RCA binding resulted in a more open and disordered CH structure. Centaureidin-3-O-glucoside (C3G) exhibited high affinity for CH, with a binding ratio close to 1.5:1. 1H nuclear magnetic resonance confirmed that the main interaction sites with CH were at the C3G A- and C-rings. This study clarifies how protein hydrolysates protect against anthocyanin degradation from experimental and theoretical aspects.

ВЛИЯНИЕ ГИДРОЛИЗАТА КОЛЛАГЕНА НА КИНЕТИКУ ВУЛКАНИЗАЦИИ И ПЛОТНОСТЬ СШИВАНИЯ РЕЗИН НА ОСНОВЕ БУТАДИЕН-НИТРИЛЬНОГО КАУЧУКА БНКС-18

The effect of collagen hydrolysate (CH) obtained from the swim bladder of northern fish species on the set of operational properties of elastomers based on BNKS-18 nitrile butadiene rubber with acrylonitrile content of 18 wt.% was studied. The vulcanization kinetics of elastomeric blends were determined on a D-RPA 3000 rotorlessrheometer (MonTech, Germany). Tensile strength and elastic properties of vulcanizates were determined on an Autograph AGS-JSTD testing machine (Shimadzu, Japan). Frost resistance was evaluated by the value of frost resistance coefficient according to elastic rebound after compression at -20, -30, -40, -50 °C. The density of the vulcanization network of the studied elastomers was determined according to the Flory-Rener equation by the method of equilibrium swelling of vulcanizates in chloroform. The analysis of vulcanization kinetics showed that the introduction of collagen hydrolysate obtained by us into the composition of BNKS-18 based elastomer increased the minimum torque. That is, the viscosity of the studied elastomers increased before the start of the main vulcanization process. As demonstrated in this study, CH significantly affects the crosslinking density of elastomer. Introduction of CH in the composition of elastomers leads to the formation of a sufficiently dense and strong vulcanization network. This is apparently associated with the fact that CH fragments interact with the ingredients of the vulcanizing system and the rubber macromolecules, participating in the structuring of the material. It was found that the introduction of CH increased modulus at 100% elongation and tensile strength, while elongation at break decreased. The maximum value of tensile strength corresponds to the introduction of 3phr of CH and amounts to 16.9 MPa, which is 37% higher in comparison with control elastomers without CH. It was shown that the increase of crosslink density of vulcanization network of the obtained elastomers was accompanied by increase of frost resistance coefficient at -30 and -40 °C. The field of application of the developed materials is manufacturing of rubber seals, cuffs, oil seals, gaskets designed for the Far North and the Arctic equipment.

Effect of collagen hydrolysate on bread quality

Effect of collagen hydrolysate on bread quality

Effects of Collagen Hydrolysate From Large Hybrid Sturgeon on Mitigating Ultraviolet B-Induced Photodamage.

Ultraviolet B (UVB) radiation leads to the excessive accumulation of reactive oxygen species (ROS), which subsequently promote inflammation, degradation of the extracellular matrix, and photoaging in skin. Thus antioxidant activity is particularly important when screening for active substances to prevent or repair photodamage. Marine fish-derived bioactive peptides have become a trend in cosmetics and functional food industries owing to their potential dermatological benefits. In this study, 1-diphenyl- 2-pycryl-hydrazyl (DPPH) scavenging activity was selected to optimize the hydrolysis conditions of sturgeon skin collagen peptides with antioxidant activity. The optimal hydrolysis conditions for sturgeon skin collagen hydrolysate (SSCH) were determined by response surface methodology, which comprised an enzyme dosage of flavorzyme at 6,068.4 U/g, temperature of 35.5°C, pH of 7, and hydrolysis time of 6 h. SSCH showed good radical-scavenging capacities with a DPPH scavenging efficiency of 95%. Then, the effect of low-molecular-weight SSCH fraction (SSCH-L) on UVB irradiation-induced photodamage was evaluated in mouse fibroblast L929 cells and zebrafish. SSCH-L reduced intracellular ROS levels and the malondialdehyde content, thereby alleviating the oxidative damage caused by UVB radiation. Moreover SSCH-L inhibited the mRNA expression of genes encoding the pro-inflammatory cytokines IL-1β, IL-6, TNF-α, and Cox-2. SSCH-L treatment further increased the collagen Ⅰα1 content and had a significant inhibitory effect on matrix metalloproteinase expression. The phosphorylation level of JNK and the expression of c-Jun protein were significantly reduced by SSCH-L. Additionally, SSCH-L increased the tail fin area at 0.125 and 0.25 mg/ml in a zebrafish UVB radiation model, which highlighted the potential of SSCH-L to repair UVB-irradiated zebrafish skin damage. Peptide sequences of SSCH-L were identified by liquid chromatography-tandem mass spectrometry. Based on the 3D-QSAR modeling prediction, six total peptides were selected to test the UVB-protective activity. Among these peptides, DPFRHY showed good UVB-repair activity, ROS-scavenging activity, DNA damage-protective activity and apoptosis inhibition activity. These results suggested that DPFRHY has potential applications as a natural anti-photodamage material in cosmetic and functional food industries.

Molecular Weight Analysis of Blue Shark (Prionace glauca) Collagen Hydrolysates by GPC-LS; Effect of High Molecular Weight Hydrolysates on Fibroblast Cultures: mRNA Collagen Type I Expression and Synthesis.

High molecular weight (Mw) collagen hydrolysates have been demonstrated to produce a higher synthesis of collagen type I mRNA. Mw determination is a key factor maximizing the effect of collagen hydrolysates on collagen type I synthesis by fibroblasts. This work aimed to achieve a high average Mw in Blue Shark Collagen Hydrolysate, studying different hydrolysis parameters by GPC-LS analysis and testing its effect on mRNA Type I collagen expression. Analysis revealed differences in blue shark collagen hydrolysates Mw depending on hydrolysis conditions. Papain leads to obtaining a significantly higher Mw hydrolysate than Alcalase at different times of hydrolysis and at different enzyme/substrate ratios. Besides, the time of the hydrolysis factor is more determinant than the enzyme/substrate ratio factor for obtaining a higher or lower hydrolysate Mw when using Papain as the enzyme. Contrary, Alcalase hydrolysates resulted in similar Mw with no significant differences between different conditions of hydrolysis assayed. Blue shark collagen hydrolysate showing the highest Mw showed neither cytotoxic nor proliferation effect on fibroblast cell culture. Besides, it exhibited an increasing effect on both mRNA expression and pro-collagen I production.

The effect of collagen hydrolysates from silver carp (Hypophthalmichthys molitrix) skin on UV-induced photoaging in mice: molecular weight affects skin repair.

The use of collagen hydrolysates (CHs) as a nutraceutical agent in skin aging has gained increasing attention. Here, the effects of various doses and molecular weights of CH from silver carp skin on photoaging in mice were investigated. The ingestion of CH at 50, 100 and 200 mg per kg body weight led to a dose-dependent increase in the hydroxyproline, hyaluronic acid and moisture contents of the skin, but it had no significant effect on the mice body weight, or on the spleen or thymus index. Furthermore, ingesting CH with lower (LMCH, 200-1000 Da, 65%) and higher molecular weight (HMCH, >1000 Da, 72%) significantly increased the skin components and improved the antioxidative enzyme activities in both serum and skin (p < 0.05); LMCH performed better than HMCH. By contrast, gelatin (>120 kDa) ingestion did not bring a significant change compared to model mice. These results indicated that LMCH exerted a stronger beneficial effect on the skin than did either HMCH and gelatin, which supported the feasibility of using LMCH as a dietary supplement from silver carp skin to combat photoaging.

The effect of collagen hydrolysates on the activity of aggrecanases and the release of proteoglycans from human osteoarthritic articular cartilage

The effect of collagen hydrolysates on the activity of aggrecanases and the release of proteoglycans from human osteoarthritic articular cartilage

Effect of collagen hydrolysate in articular pain: A 6-month randomized, double-blind, placebo controlled study

Evaluation of the efficacy and safety of a food supplement made of collagen hydrolysate 1200 mg/day versus placebo during 6 months, in subjects with joint pain at the lower or upper limbs or at the lumbar spine. Comparative double-blind randomized multicenter study in parallel groups. 200 patients of both genders of at least 50 years old with joint pain assessed as ≥30 mm on a visual analogical scale (VAS). Collagen hydrolysate 1200 mg/day or placebo during 6 months. Comparison of the percentage of clinical responder between the active collagen hydrolysate group and the placebo group after 6 months of study. A responder subject was defined as a subject experiencing a clinically significant improvement (i.e. by 20% or more) in the most painful joint using the VAS score. All analyses were performed using an intent-to-treat procedure. At 6 months, the proportion of clinical responders to the treatment, according to VAS scores, was significantly higher in the collagen hydrolysate (CH) group 51.6%, compared to the placebo group 36.5% (p<0.05). However, there was no significant difference between groups at 3 months (44.1% vs. 39.6%, p=0.53). No significant difference in terms of security and tolerability was observed between the two groups. This study suggests that collagen hydrolysate 1200 mg/day could increase the number of clinical responders (i.e. improvement of at least 20% on the VAS) compared to placebo. More studies are needed to confirm the clinical interest of this food supplement.

Effect of Collagen Hydrolysates from Salmon and Trout Skins on the Lipid Profile in Rats

The effect of collagen hydrolysates from fish skins on lipid profile was assessed in rats administered chum salmon or rainbow trout collagen peptide. Single oral administration of soybean oil with or without one of either type of fish collagen peptide demonstrated that rat plasma triglycerides were significantly decreased 2 h later after the intake of oil and peptide mixtures (p < 0.05). The free and peptide forms of hydroxyproline derived from fish collagen peptides were statistically higher than those of zero time after oral administration. To test the effect of fish collagen peptides on continuous administration, rats were fed an AIN-93G purified diet containing 0.17% fish collagen peptide. The peptide groups had lower levels of plasma total lipids and triglycerides compared with the control group. However, the body, liver, and fat weights of rats were not significantly different between groups. These results suggest that fish collagen hydrolysates affect lipid absorption and metabolism in rats and may be useful in suppressing the transient increase of plasma triglycerides.

Effect of collagen hydrolysate on chondrocyte-seeded agarose constructs

The mechanical properties of engineered cartilage are strongly dependent on collagen content, but the collagen to glycosaminoglycan ratio in engineered cartilage is often much lower than that of the native tissue. Therefore culture medium supplements which increase collagen production by chondrocytes are of interest. It had previously been reported that collagen hydrolysate stimulated type II collagen biosynthesis in short-term, high density monolayer chondrocyte cultures. It was hypothesized that collagen hydrolysate added to the culture medium of three dimensional chondrocyte-agarose constructs would enhance their mechanical properties. Porcine articular chondrocytes were embedded in 2% agarose and cultured for up to 6 weeks with and without 1 mg/ml collagen hydrolysate. The instantaneous compressive modulus and equilibrium compressive modulus were significantly lower in the collagen hydrolysate-treated constructs, consistent with the finding of lower collagen and GAG content. Contrary to our hypothesis, our results indicate that 1 mg/ml collagen hydrolysate may actually inhibit macromolecule biosynthesis and be detrimental to the mechanical properties of long term chondrocyte-agarose constructs.

Blends of polyvinylalcohol with collagen hydrolysate: properties of water-soluble blown films

The study is focused on the effect of collagen hydrolysate and glycerol on properties of water-soluble polyvinylalcohol (PVA) blown films and blends. The experimental design method (DOE) was used in this study. Results from DOE show a strong effect of interactions between the blend components on resulting properties of the films. This fact was confirmed by means of the response surfaces of melting flow index, tensile strength and the content of insoluble fraction. It was concluded that the composition of PVA/CH blend must be precisely specified to avoid the processing complications.