N(trichloromethylthio) -4-cyclohexene-1, 2-dicarboximide (captan)3 is an important member of a class of organic fungicides which has been synthesized by the reaction of perchlorome-thyl mercaptan with certain imides, amides, and hydantoins (Kittleson, 1952). All the compounds of this class have in common the N-trichloromethylthio group to which their activity has been attributed (Kittleson, 1953). Captan is currently used extensively in the control of certain fungus diseases of plants. Studies of certain effects of captan on fungus metabolism reported in this paper were undertaken to elucidate the mode of action of this fungicide. MATERIALS AND METHODS.-Captan 4 was purified from a technical sample by precipitation from acetone with water and had an uncorrected melting point of 172?C. Inasmuch as captan is of limited solubility in water, a wetting agent 5 was used to prepare fine suspensions which could be pipetted accurately: The final concentration of wetting agent (0.00125 per cent) had no measurable effect on the biological materials used. The fungus, Fusarium roseum Lk., was used as a test organism and was grown on a medium containing: maltose, 10 g.; proteose peptone, 5 g.; KH2PO4, 0.5 g-; MgSO4. 7H20, 0.5 g.; FeSO4. 71120, 0.03 g-; agar, 20 g.; and distilled water, 1 1. Conidia were harvested by washing them from the surface of cultures which were 7 days old. The conidial suspensions were freed, of mycelial fragments by passage through several layers of cheese cloth, and the conidia were washed 3 times by alternate centrifugation and resuspension. For use in manometric experiments, the conidia were suspended in a basal solution containing: 0.002 M MgSO4 7H20 and 0.0001 M FeSO4 7H20 and potassium phosphate buffer (0.04 M
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