The experiment was carried out at Research Field of Horticulture Research Farm, Naini Agricultural Institute, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj (U.P.) India, during Rabi Season 2023-2024 to investigate twelve treatments viz., T0 (RDN (Control), T1 (RDN + Azosprillum@7 kg/ha), T2 (RDN + Phosphate Solubilizing Bacteria @6kg/ha), T3 (RDN + VAM @10kg/ha), T4 (RDN + Azotobacter@7kg/ha), T5 (RDN + Azospirillum@7kg/ha + PSB@ 6kg/ha), T6 (RDN + Azospirillum@7kg/ha + VAM@10kg/ha), T7 (RDN + PSB@6kg/ha + VAM@ 10kg/ha) and T8 (RDN + PSB@6kg/ha + Azotobacter@7kg/ha), T9 (RDN + Azotobacter@7kg/ha + VAM@10kg/ha) , T10 , (RDN+Azotobacter@7kg/ha+ PSB@6kg/ha + VAM@10kg/ha) and T11 (RDN + Azospirillum@7kg/ha + PSB@6kg/ha + VAM@10kg/ha)along with their combinations, replicated thrice in a Randomized Block Design. In strawberry use the application of bio-fertilizer like Azotobacter and Azospirillum hasten early flowering along with the expanded duration of blossoming, harvesting by increasing the growth, yield and quality of strawberry. The integrated nutrient management maximized plant height (cm), number of leaves per plant, plant spread (E-W, N-S) (cm), number of flowers per plant, number of fruit per plant, fruit weight (g), fruit length (cm), fruit diameter (cm), fruit yield (q/ha), total soluble solids, ascorbic acid (mg/100g). From the above experimental finding it may concluded that, the production of strawberry by the bio fertilizer application of T11 (RDN + Azospirillum@7kg/ha + PSB@6kg/ha + VAM@10kg/ha) was found to best in terms of growth, yield and quality. In the investigation improves vegetative growth of flowering and improve yield by the help of bio fertilizer such. The highest net return was also found in the T11 and highest B:C ratio was found in T10 with 3.64.