Effect Of 6-benzylaminopurine Research Articles

Effects of benzyl amino purine on growth, antioxidants, and chlorophyll contents of Phaseolus vulgaris L. cultivated under heat stress

Effects of benzyl amino purine on growth, antioxidants, and chlorophyll contents of Phaseolus vulgaris L. cultivated under heat stress

Effects of benzylaminopurine and liquid fertilizer application on lateral branch development and the yield of raspberry ‘Heritage’

Effects of benzylaminopurine and liquid fertilizer application on lateral branch development and the yield of raspberry ‘Heritage’

Effect of Benzyl Amino Purine (BAP) on the Leaf Growth of Vanda limbata Blume Orchid In Vivo

<p><em>Vanda limbata</em> is a natural orchid with slow growth and is often taken directly from the forest in large quantities, threatening its sustainability. Ex-situ preservation efforts are needed for this orchid, one of which is through cultivation with the addition of Benzyl Amino Purin (BAP), known to increase shoot growth. This research aims to analyze the effect of BAP and determine its optimal concentration on the growth of <em>V. limbata</em> orchids. This research used a single-factor completely randomized design in BAP concentrations of 0, 25 and 50 mg l<sup>-1</sup> with 5 replications. The method used was spraying BAP solution once a week on all parts of adult <em>V. limbata</em> orchids aged ±2 years. The research was conducted for 3 months in the experimental garden with parameters observed in the form of the number of new leaves, elongation of old leaves, the length of new leaves, increase in the width of old leaves, the width of new leaves, stomatal density and the number of roots. The results showed that the most effective BAP concentration was 50 mg l<sup>-1</sup> for the number of new leaves which increased by 200%, the length of old leaves which increased by 168.66% and the length of new leaves which increased by 800%, but resulted in less stomatal density than the control. BAP can increase the growth of <em>V. limbata</em> orchid leaves. This research can be a recommendation for ex-situ conservation efforts that have the potential to be developed as a means of orchid cultivation.</p>

Effects of benzylaminopurine and macro propagation techniques on multiplication of dessert banana (Musa acuminate L.)

Effects of benzylaminopurine and macro propagation techniques on multiplication of dessert banana (Musa acuminate L.)

Effect of 6-Benzylaminopurine (BA) and Paclobutrazol (PBZ) with Light Intensity Variations for Cherry Tomatoes In vitro Flowering

Utilization of Solanum lycopersicum L. and its varieties contribute to plant biotechnology to study the effect of genes and flower transition events. This study aims to optimize media and light intensity for in vitro flowering of a cherry tomato variety. Induction of flowering was carried out using seven types of media combined with 3 light intensities, followed by feeding by adding liquid medium MS+PBZ 1 mg/L. A positive response is shown at the light intensity of 2,600 lux; there are 5 explants that have flower buds. The percentage of flower buds for each treatment combination was 50% from two replications. The first shoots occurred at 142 days after planting (DAP) on explants grown on media containing 0.5 mg/L BA, 0.75 mg/L PBZ, with feeding treatment. It can also be assumed that the emergence of flower buds occurred not because of the PGR induction treatment, but because at that time the explants had entered the generative phase, considering that the plantlets had been growing for a long time. Although the effects of BA and PBZ have not been confirmed, the use of 2,600 lux light appears to be the best condition for cherry tomato in vitro flowering induction.

Effect of 6-benzyl amino purine on the multiplication ability of shoots of various sizes of porang (Amorphophallus muelleri Blume) bulbils

Porang plant is very prospective and developed because it has high economic value. The high demand for exports has resulted in increased needs for seeds. Tissue culture using plant regulator 6-Benzyl Amino Purine (BAP) has been widely used for seed propagation. This study aimed to examine the effect of adding BAP on the multiplication of shoots at different bulbil weights. Bulbils (corm leaf) were used as explants and cultured on Murashige and Skoog medium containing BAP (according to treatment), 30 g L−1 sucrose, and 2.5 g L−1 phytagel. This study used two factors Completely Randomized Design with five replications. The first factor was concentrations of BAP i.e., 0.0, 1.0, 2.0 and 3.0 mg L−1 and second factor was bulbil weight i.e., 0.25, 0.50 and 1.00 g. There was significant interaction at the number of buds and shoots, but not for other parameters. The results showed that bulbil weight had a significant effect on the number of buds, shoots, and plantlet height, while the concentration of BAP had a significant effect on all parameters. Bulbil size < 1 gram can reproduce seeds with the highest multiplication ability when 3 mg L−1 BAP was given.

PLANT TISSUE CULTURE AND CALLUS INDUCTION FROM COTYLEDONARY SEGMENTS OF CUCURBITA MAXIMA (L)

The efficiency of regeneration from Cotyledonary segments was compared by containing the number of shoots MS basal medium with growth regulaters BAP 0.5mgl NAA and Kn, 0.5 mgl/L to 3.0 mgl/L, LGlutamic acid and CM Coconut milk 3.0mgl/L the regeneration MS contained medium 2.0 mgl/L BAP and 2.0 mgl/L L-Glutamic acid NAA Callus, shoot and plant regeneration in Cucurbita maxima the successful establishment of In Vitro requirements for plant regeneration from Cucurbita maxima Cotyledonary nodal segments. The nodal explants were inoculated on MS basal medium supplemented with various cytokinins i.e., BAP and NAA. Coconut water also had a role in triggering the formation of multiple shoots. Addition of BAP at 2.0 mg/l concentration or NAA at 3.0 mg/l to the MS basal medium, induced regeneration from the nodal segments Trichosanthes Cucumeriana. In Vitro seeding derived callus saussurea (Joshi 2003, Wawro Sch 2001). Elimination of viruses fro fruit trees (Knapp et al 1995, Cieslinska 2002) Shoot induction was achieved in one of the important medicinal plant of cucurbitacae family, Cucurbita maxima. MS medium supplemented with 1.0 mg/l BAP + 2.0 mg/l NAA and 2.0 mg/l L-Glutamicacid was found to be optimum to induce shoots. The present study established reliable and reproducible protocol for rapid multiple shoot induction from Cotyledonary explants of Trichosanthes Cucumeriana using different concentrations and combinations of cytokinins. Tissue culture techniques are now becoming popular as alternative means of vegetative propagation. The effect of benzyl amino purine in inducing shoot induction was already reported in some of the important medicinal plants (Komalavalli and Rao, 2000). An effective and reproductive procedure for the regeneration of shoots and plantlets from callus and in vitro tissue culture technique is essential in studies involving gene transfer. Somewhat greater success in regeneration of bean from organ cultures Malik and Sexena (1991) protoplast Isolation from leaf explants of Solanum Surattense A Ramulu, et al (2014) Phytochemical analysis and biological activates in Solanum Surattense Venakteshwarlu et al (2018)

In Vitro Culture of Rosmarinus officinalis L. in a Temporary Immersion System: Influence of Two Phytohormones on Plant Growth and Carnosol Production.

Emerging infectious diseases have become a major global problem with public health and economic consequences. It is an urgent need to develop new anti-infective therapies. The natural diterpene carnosol exhibit a wide variety of interesting antibacterial and antiviral properties, and it is considered a theoretical inhibitor of COVID-19 Mpro. However, this compound is present in the family Lamiaceae in low quantities. To obtain carnosol in concentrations high enough to develop pharmacological studies, we evaluated the efficiency of a micropropagation protocol of Rosmarinus officinalis using a solid medium and a temporary immersion system (TIS), as well as the effect of 6-benzylaminopurine (6-BAP) and α-naphthaleneacetic acid (NAA) on the growth of shoots. Moreover, we developed and validated an analytical method to quantify carnosol using the H-point standard additions method in the high-performance liquid chromatography diode array detector (HPLC-DAD). After 30 days of culture, TIS produced the maximum number of shoots per explant (24.33 ± 1.15) on a liquid medium supplemented with 6-BAP at 5.0 mg L−1. Next, we also evaluated the effect of immersion time and frequency for TIS. After 72 days of culture, the best results were obtained with an immersion cycle of 1 min every 12 h, yielding 170.33 ± 29.40 shoots. The quantification of carnosol on the samples was performed at a flow rate of 1.2 mL min−1 using binary isocratic mobile phase system 60:40 (v/v) 10 mM formic acid (pH 3.0) (A) and acetonitrile (B) on a reverse-phase column. The content of carnosol in the in vitro cultures was around 8-fold higher than in the wild plant. The present study represents an efficient alternative method to obtain carnosol for its pre-clinical and clinical development.

Optimization of the micropropagation of elite adult trees of Sequoia sempervirens: forest species of interest in the Basque Country, Spain

Forest trees are renewable sources of timber and other valuable non-timber products. Nowadays, the increase in population and demand for forestry products results in overexploitation of forestry. Therefore, there is an urgent need to produce elite plants with higher productivity under stress derived from climate change to have available to afforestation. For this reason, propagation methods should be improved to be more efficient in terms of quality and productivity. The main species planted in the Basque Country is Pinus radiata; during the last three years, Pinus radiata plantations have suffered a fungus attack affecting mainly needles until the tree's death. This crisis is caused by the combined action of two fungi of the genus Dothistroma and Lecanosticta acicola, whose expansion seems to have been enhanced by weather-related factors, such as humid and hot summers. Although we have evidence of this disease's presence in our mountains since 1942, the disease has had a speedy expansion with an aggressive effect for reasons that are not scientifically known today. For the above, Basque Country forestry sector is looking for alternative species to be used in its plantations. Part of the forestry sector considers that Sequoia sempervirens could be a good choice for plantations. Besides, its high-quality wood and its tolerance to the attack of several pathogens and other diseases derived from climate change are characteristics that could confer some advantage over other forest species. The main goal of this study was to optimize the micropropagation of adult elite trees of Sequoia sempervirens. The effect of 6-benzylaminopurine, meta-Topolin and Kinetin, and 4 types of explant in the multiplication stage were analyzed to carry out this objective. Furthermore, the effect of two types of auxins: 1-naphthalene acetic acid, indole-3-butyric acid, and a mixture of both, were evaluated on the induction of roots and their subsequent effect on the acclimatization process. The best multiplication index was obtained when 4.4 µM 6-benzylaminopurine and apical explants longer than 1.5 cm of length were used. The root induction percentage was 75% in the most responsive genotype analyzed when 4.4 µM 6-benzylaminopurine was used on the induction stage, and 50 µM of 1-naphthalene acetic acid was used for rooting. Finally, after 3 months in the greenhouse, the explants cultured with Kinetin and rooted in a culture medium with indole-3-butyric acid showed the highest acclimatization success (94%).

The Effect of Benzyl Amino Purine (BAP) Concentration on the Growth Amount of the Explant of Dendrobium spectabile Orchid by In-Vitro

&lt;em&gt;Dendrobium&lt;/em&gt;&lt;em&gt; spectabile orchid is an ornamental plant of high economic value, having relatively durable properties, beauty, and attractiveness of orchids lie in the diverse shapes and colors of the flowers. Therefore, it requires rapid and efficient propagation techniques. One of them is in-vitro vegetative propagation. Plant propagation using the in-vitro technique has the opportunity to produce a large number of plant seeds in a relatively short time. This study aims to determine the correct concentration of Benzyl Amino Purine (BAP) on the growth amount of the explants of Dendrobium spectabile orchid by in-vitro. The research was conducted at the Biotechnology Laboratory of the Regional Technical Implementation Unit (UPTD) of the Aloe Vera Center (AVC) Pontianak. This research was conducted from January 1, 2020, to March 30, 2020. The method used was a Completely Randomized Design (CRD). The treatment consisted of 6 levels of treatment, namely b1 = 0.75 ppm, b2 = 1.00 ppm, b3 = 1.25 ppm, b4 = 1.50 ppm, b5 = 1.75 ppm, b6 = 2.00 ppm. All treatments were repeated 4 times for each treatment consisting of 3 plant samples. The research finding showed that the treatment of BAP concentration 1.50 was the best in increasing the average number of leaves 4.92 strands, the average number of shoots 4.25 buds, and the average leaf length was 2.44 cm.&lt;/em&gt;

Effect of Benzylamino Purine (BA) and Gibberellic Acid (GA3) on Growth and Flowering in Schlumbergera truncata

Effect of Benzylamino Purine (BA) and Gibberellic Acid (GA3) on Growth and Flowering in Schlumbergera truncata

Effect of 6-Benzylaminopurine on Morphogenesis and Production Process of Sweet Pepper (Capsicum annuum L.)

Effect of 6-Benzylaminopurine on Morphogenesis and Production Process of Sweet Pepper (Capsicum annuum L.)

Media optimization and the effect of benzyl amino purine (BAP) and gibberellic acid (GA3) on the shooting of Pterocarpus erinaceus Poir.

Ptreocarpus erinaceus is a very useful forest tree due to its economic and medicinal values. It is also used as fodder for livestock due to its high nitrogen content. Its numerous use attract the high demand leading to it’s over exploitation. However, the tree is slow growing in nature. Hence, there is a need to look for in vitro methods for its mass propagation as an alternative method to raise healthy trees and sustain its availability. Media optimization gave two best performing media; Driver and kuniyuki Walnut (DKW) and Murashige and Skoog MS) which were further supplemented with different combinations of plant growth regulators (PGR)-Benzyl Amino Purine (BAP) and Gibberellic Acid (GA3). Overall, the DKW media with BAP/GA3 at 1.0/0.5 produced the highest shoot length while BAP/GA3 at 4.0/0.0 gave the highest number of leaves after eight weeks of inoculation. Effective utilization of these experimental conditions could be the impetus towards mass propagation of this important tree species.&#x0D; Keywords: Ptreocarpus erinaceus, media optimization, Plant growth regulator, shoot length, mass propagation

Effect of benzyl amino purine and 1-methylcyclopropene in maintaining rooting quality of chrysanthemum (Chrysanthemum morifolium Ramat cv. ‘White Fiji’) cuttings

The quality of chrysanthemum cuttings is influenced by ethylene. During postharvest storage, ethylene accelerates the quality loss of cutting by enhancing chlorophyll degradation. Application of 1-Methylcyclopropene (1-MCP) or Benzyl Amino Purine (BAP) can be used as an approach to inhibit unfavourable ethylene effects. The objective of this study was to find out the effect of a combination of 1-MCP or BAP and storage period on the quality of chrysanthemum cuttings. The experiments were arranged using a completely randomized design consisting of nine treatments. The cuttings were treated with 1-MCP 700 nL/L or BAP 2.5 mg/l. After treatment, they were stored for 0, 3 and 7 days, then the cuttings were rooted in soilless culture for four weeks. The results showed that 1-MCP or BAP maintained leaf freshness and inhibited chlorophyll degradation on cuttings at 3 and 7 days of storage period. However, the application of 1-MCP slightly inhibited root growth compared to that of untreated cuttings. Interestingly, the application of BAP effectively reduced the cuttings damage for seven days and did not cause the inhibition of root growth (weight of fresh root, root number, root length and root volume) that was comparable to that of untreated cuttings. These results collectively showed that BAP was more effective than 1-MCP in maintaining rooting quality of chrysanthemum cuttings.

Effect of 6-Benzylaminopurine and Honey for in Vitro Shoot Initiation of Mangosteen Seed Explant From Riau, Indonesia

Mangosteen (Garcinia mangostana L.) is a tropical plant that has export value because it has a rich of antioxidant xanthone that can be used for medicine as an anti-cancer agent. Mangosteen (Garcinia mangostana L.) from Riau, Indonesia has many superiorities such as grow in the marshes and tolerant to acid soil (peat soil). One of the alternative methods to produce large number and uniform mangosteen seedlings in a short period can be done using in vitro culture technique. The purpose of this research was to determine the optimal concentration of BAP and honey in shoot initiation of mangosteen explant from seed that had been divided into four parts and also to determine the best combination for initiation mangosteen shoots using in vitro method. This research used a Completely Randomized Design (CRD) with the concentration of BAP (3; 7 mg/l) and honey (3; 6; 9 ml/l) or in combination on MS medium with five replications. The result showed that the highest number of shoots (14.60) formed on MS medium supplement with 7 mg/l BAP + 3 ml/l honey. The combination between 3 mg/l BAP + 3 ml/l honey produced the tallest shoots (5.10 cm) and the highest number of leaves (3.20 leaves) on MS medium supplement with 3 mg/l BAP + 9 ml/l honey. In this study, we have successfully presented the effect of 6-benzyl amino purine and honey for in vitro shoots initiation from mangosteen seed explant (Garcinia mangostana L.) which taken from Riau Indonesia.

English

In Asia, Radix Bupleuri (Bupleurum spp. root) is an important medicine used for treating many diseases over the past 2000 years. However, its cultivation is difficult due to poor germination rates and loss of seedling viability shortly after seeding. In the present study, the effect of 6-benzyl aminopurine (6-BA), daminozide, gibberellin (GA3), paclobutrazol, uniconazole, and mepiquat chloride (MC) on the germination of seeds of ‘Zhongchai No. 3’, which is the newest released commercial variety of Bupleurum chinense DC in China was analyzed. Only GA3 was found to promote seed germination significantly, and its optimum concentration was 223.84 to 238.78 mg∙L-1. In addition, incubation with GA3 on the first day of germination was extremely important for seedling survival. This protocol could also be applied to ‘Zhonghongchai No. 1’ (B. scorzonerifolium Willd.) and ‘Hubei chaihu’ (B. chinense DC.). The combination of GA3 treatment and centralized incubation could be an efficient way to industrialize the production of secondary metabolites from Bupleurum species. Key words: Hormone, Bupleurum spp., germination, gibberellin (GA3).

The influence of different plant hormones on biomass and starch accumulation of duckweed: A renewable feedstock for bioethanol production

Duckweed has been considered as a renewable feedstock for bioethanol production due to its fast generation of biomass and high starch accumulation. The use of plant hormones is a common and efficient method to manipulate plant growth and yield. However, the effects of different plant hormones on the biomass and starch accumulation of duckweed have not been systematically studied. Here, we screened five classes of plant hormones, including auxin, cytokinin, abscisic acid (ABA), gibberellins, and brassinosteroids. The results showed that the effect of 6-benzylaminopurine (6-BA, cytokinin) on promoting the biomass production of duckweed was much higher than other plant hormones. Compared with the control group, biomass yield at 1.0 × 10−3 mM 6-BA treated group increased by 37.41% in 7 days. Moreover, the results suggested that ABA can dramatically promote starch accumulation. The total starch that accumulated in the ABA treated samples were 3.3 times higher than that in the control samples. Taken together, these findings indicated that 6-BA and ABA were the most effective plant hormones in terms of enhancing biomass and starch accumulation. These results will provide valuable information for further studies of large-scale application of plant hormones in duckweed-to-bioethanol production in the future.

PENGARUH BAP (Benzyl Amino Purine) DAN AIR KELAPA TERHADAP PERTUMBUHAN TUNAS PUCUK DAN KANDUNGAN SULFORAFAN BROKOLI (Brassica oleracea L. var. italica Plenck) SECARA IN-VITRO

This study aims to know the effect of Benzyl Amino Purine (BAP) and Coconut Water on shoot bud growth and Broccoli Sulforaphane content (Brassica oleracea L. var italic Plenck). The study was conducted in the laboratory of Biotechnology Department of Aquaculture, Faculty of Agriculture of Sam Ratulangi University, Manado, that conducted from August-December 2017. This study used a Complete Randomized Design (RAL), consisting of 8 treatments and each repeated as many 4 times, so we get 32 unit experiment. The variables observed were number of buds, number of leaves, plant height, wet weight, root number and Sulforaphane content analysis. The result of research shows that analysis of variance showed that in the use of Benzyl Amino Purine (BAP) concentration 3 ppm tends to increase the number of leaves aged 4 Weeks After Culture (MSK) and increase the number of shoots age 2 and 6 Weeks After Culture (MSK). Benzyl Amino Purine (BAP) 3 ppm can increase the wet weight of age 6W eeks After Culture ((MSK). Coconut water 20% tends to increase the number of leaves at age 6 Weeks After Culture (MSK) and increase the number of shoots aged 6 Weeks After Culture (MSK), while for combination of 3 ppm Benzyl Amino Purine (BAP) and coconut water 20% tends to increase the number of leaves aged 2 Weeks After Culture (MSK) and the number of shoots aged 2 Weeks After Culture (MSK). Combination of coconut water and Benzyl Amino Purine (BAP) is not detected by the content of Sulforaphane.

Effect of benzyl amino purine and indole-3-acetic acid on propagation of Sterculia foetida in vitro

Sterculia foetida is an oval seed plants that can be used as biofuel, which is one of the environmental friendly fuels. This plant is quite hard to find because not many peoples cultivate the plants. An in vitro propagation is one way to preserve the plant. This research aimed to determine optimum concentration of benzyl amino purine (BAP) and indole-3-acetic acid (IAA) to propagate S. foetida in vitro. The results showed that woody plant medium (WPM) added by 4 mg L BAP-1 and 0.5 mg L IAA-1 was able to produce complete plantlet, whereas those added by 4 mg L BAP-1 and 1 mg L IAA-1 generated the best growth of shoot and leaves.

IN VITRO MULTIPLICATION OF JABON (ANTHOCEPHALUS CADAMBA (ROXB)) ON VARIOUS CONCENTRATION OF BAP AND IAA

One of problems in the Jabon propagation is the availability of seeds both quality and quantity. Tissue culture technology is one of the alternatives that can be used for the supply of Jabonseeds to produce organs of plants (buds, leaves, roots). The success of plant tissue culture techniques is determined by the condition of explants, a supportive environment and the addition of growth regulators are expected to provide a response to the cultured explants. This study aimed to know the effect of Benzyl Amino Purine (BAP) and Indole Acetic Acid (IAA) to in vitro multiplication of Jabon. This research conducted at the Laboratory of Forestry Sciences, Faculty of Forestry, University of Tadulako from March to May 2015. Using a completely randomized design with treatments: 0.1 mg / l IAA + 1 mg / l BAP (JB1), 0.1 mg / l IAA + 1.5 mg / l BAP (JB2), 0.1 mg / l IAA + 2 mg / l BAP ( JB3) and 0.1 mg / l IAA + 2.5 mg / l BAP (JB4). Each treatment was repeated three times to obtain 12 experimental units. The results showed that the difference in response Jabon of treatment tested was the highest number of buds and leaves were in the media added 0.1 mg / l IAA + 1.5 mg / l BAP. Furthermore, the formation of callus obtained in media which added 0.1 mg / l IAA + 1 mg / l BAP.