Aim: The coronavirus disease 2019 (COVID-19) has become a rapidly spreading pandemic all over the World. Social isolation is the most effective prevention option after vaccination. Many habits have changed in our lives due to social isolation during the pandemic. In terms of education, globally, the traditional education system has turned into an online education system. Physiology is a compulsory course that has an important place in health education and is a fundamental science for understanding pathophysiology, health assessment, and pharmacology. With the transition to the online education system, it is necessary to determine the effectiveness of physiology education. The aim of this study is to determine the effectiveness of online education and face-to-face education on students in physiology education and to measure students' satisfaction.Methods: The research was conducted with 916 students studying at 29 universities in different provinces of Türkiye through an online survey. This survey was self-designed based on a 3-point Likert scale. The survey was applied to 50 students and the questions in the form were finalized according to the answers received. The answers given were grouped under the four titles of Demographic Information, Educational Information, Access to Online Education and Perspective on Online Education in Physiology. The obtained data were interpreted by being subjected to statistical analysis.Results: In the comparison of online education and face-to-face education, the preferences of the students have been face-to-face education. The number of students who consider online education sufficient in understanding physiology lessons, in general, are few (n=242, 26.4%), those who see it incompletely are more (n=367, 40.1%), while 1/3 gave neutral views (n=307, 33.5%). The majority of participants have the opinion that "face-to-face education is more effective in terms of increasing knowledge in physiology education compared to online education" and “It is more difficult for me to focus on physiology courses during online education compared to face-to-face education”.Conclusions: The perceived stress level of medical school students who experienced the summative type online exam for the first time was found to be high before and after the exam. Online exam methods will be a field that is frequently applied and developed in the coming years. For this reason, there is a need for more studies in which the factors that cause stress in medical students during online exams are determined.