The integration of mobile learning technologies in biology education presents a novel approach to enhancing digital literacy among students. This study investigates the impact of mobile learning tools, such as educational apps and virtual labs, on developing digital competencies in secondary school biology lessons. Employing a mixed-methods research design, the study combines quantitative surveys to assess changes in students' digital literacy levels with qualitative interviews to gain insights from educators and students. Participants included secondary school students and biology teachers from various schools, who integrated mobile learning technologies into their biology curriculum. The results demonstrate a marked improvement in students’ digital literacy, with a notable increase in their ability to navigate, evaluate, and create information using digital technologies. Furthermore, the integration of mobile technologies was found to enhance student engagement and understanding of biology concepts. This study contributes to educational technology literature by highlighting the role of mobile learning in promoting digital literacy and providing practical insights for its implementation in biology education.