ObjectivesTo investigate the water sorption (wsp), water solubility (wsl), degree of conversion (DC), elastic indentation modulus (EIT), edge chipping resistance (ECR) and flexural strength (FS) of 3D-printed, milled and conventionally polymerized denture base resin materials. MethodsSpecimens (N = 540) were 3D-printed (NextDent Denture 3D+ (DEN), Fotodent Denture (FOT), Freeprint Denture (FRE), V-Print dentbase (VPR)), cut (Ivotion Base (IVO)) and molded (PalaXpress (PAL)) in three geometries. Wsp,wsl,DC, EIT, ECR and FS were tested initially (24 h, 37 °C, H20) and after additional aging (5000 thermal cycles, 5/55 °C). Data were analyzed with Kolmogorov-Smirnov, univariate ANOVA, Kruskal-Wallis, Mann-Whitney U test and Spearman's correlation (p < 0.05) ResultsMost 3D-printed denture base resins showed higher wsp (25.31–37.94 μg/mm3) and wsl (0.08–8.27 μg/mm3), but also higher EIT (3.11–4.09 GPa) and FS (60.81–99.57N/mm2) values than the control groups. DEN and VPR showed high DC (89.36–93.53%), EIT (3.77–4.09 GPa) and FS (79.65–99.57N/mm2), while FOT showed low wsp (25.31–27.35 μg/mm3) and wsl (1.01–3.87 μg/mm3) values. In all materials, the examined parameters were affected by aging. SignificanceAlthough 3D-printed denture base resins showed promising results with regard to the observed DC and FS, only FOT and FRE surpassed the threshold values defined by the ISO norms.