To explore the relationship between single nuclear polymorphisms (SNPs) in ectodysplasin A receptor (EDAR) and EDAR-associated death domain (EDARADD) genes and non-syndromic tooth agenesis. Ten putative SNPs in EDAR and EDARADD were selected, and a case-control study was conducted in 112 subjects with non-syndromic tooth agenesis and 112 normal control subjects. DNA was obtained from peripheral blood samples. Genotyping was performed by Sanger sequencing. Three SNPs (rs3749098, rs3749099, and rs10432616) in EDAR exhibited significant differences in the alleles and/or genotype frequencies between the case group (individuals with non-syndromic tooth agenesis) and control group (normal individuals). The T allele was identified in the SNP rs3749098 in 99.1% of the case group and in 96.0% of the control group (P = 0.0326). Regarding the SNP rs3749099, the C allele was identified in 99.1% of the case group and in 96.0% of the control group (P = 0.0326). Regarding the SNP rs10432616, the C allele was identified in 97.8% of the case group and in 100.0% of the control group (P = 0.0245). Our results suggested that SNPs in EDAR could be a pathogenic factor for non-syndromic tooth agenesis. Furthermore, EDAR can be regarded as a marker gene for the risk of tooth agenesis.