Ecosystem Level Research Articles

Fair space for life: A dynamic care monitor working up to growth and flourishing for all.

Mental health disparities persistently cause inequity and social exclusion. Extensive research underpins the need to embrace the social determinants of health and facilitate network learning at various ecosystem levels. Despite valuable quality frameworks and ratified conventions, local practices which counter health inequity are scarce. The Dutch HOP-TR study collected health and needs of Homeless Service Users (HSU) in a rights-based, transdiagnostic, recovery framework. We assessed the survival modes and conducted a socio-ecological analysis, exploring what happened in care pathways at three ecosystem levels: individual HSU, caregiver networks, society. While documenting vital conditions for growth and citizenship, we explore major opportunities to develop 'fair space for life'. Under low distress levels, prosocial behavior is prominent (32.9%). High distress levels are found with an avoidant (42.0%) or aggressive mode (24.9%). Rising distress levels give more frictions in relations, psychiatric admissions, and police-justice contacts. The distress-induced descent in the social hierarchy causes social withdrawal, alienation, and marginalization. At society level, fair conditions for growth and citizenship are challenged by the cumulative impact of distress over the HSU' lives. This care monitor uncovers the impact of distress on caregiver interactions. The care pathways reveal that the survival strategies reflect a systematic, pervasive neglect. Unfair representations hold HSU personally responsible of their situation, disregarding the cumulative impact of environmental conditions over their lives. The diverse sources of unfairness are intrinsic to the health care system and culture. Therefore, the survival modes ask for profound culture transformations in a whole-system-whole-society approach. Given the need for action on health equity and the social determinants of health, this paper provides an example of a dynamic care monitor. The actionable data elicit dialogs and stimulate to enrich opportunities for inclusion and growth in communities and societies.

Carbon Sequestration in Agricultural Soil: Technique and their Efficiency

Developing technologies to slow the rate of increase in the atmospheric concentration of carbon dioxide (CO2) from annual emissions, such as energy, process industries, land-use change, and soil cultivation, is a major challenge of the twenty-first century. Severe ecological and economic disruptions result from the erratic changes in climate systems caused by the skyrocketing amounts of atmospheric CO2 and other greenhouse gases (GHGs). Through the process of carbon sequestration, net greenhouse gas emissions can be reduced, hence mitigating climate change. Both biotic and abiotic variables can contribute to the long-term sequestration of carbon, or carbon stabilisation. The compilation of past and present methods for sequestering soil organic carbon is crucial, considering the need of measuring and tracking soil carbon at the point, field, regional, and ecosystem levels. This study attempts to provide an overview of the literature about the function of various agricultural management techniques for sequestering carbon and the ways in which they help to stabilize atmospheric carbon.

Soil water content and vapor pressure deficit affect ecosystem water use efficiency through different pathways

Ecosystem water use efficiency (WUE) quantifies the amount of carbon assimilated per unit of water consumed and elucidates the trade-off between water loss and carbon gain at the ecosystem level. Numerous studies have highlighted the significant impact of low soil water content (SWC) and high vapor pressure deficit (VPD) on WUE. However, the underlying mechanisms driving these effects differ markedly. The intensifying coupling between land and atmosphere accentuates the distinctive co-occurrence of low SWC and high VPD, posing challenges in disentangling the respective influences of these two water stressors. Consequently, a clear understanding of the independent responses of ecosystem WUE to SWC and VPD remains elusive. Using meteorological and flux data from FLUXNET’s eddy-covariance sites, we investigated the impact of SWC and VPD on ecosystem carbon–water fluxes and proposed potential pathways through which SWC and VPD influence WUE. We deconstructed WUE into intrinsic WUE of vegetation (GPP/T) and the ratio of transpiration to evapotranspiration (T/ET). Our findings revealed that WUE exhibited greater sensitivity to VPD dynamics compared to SWC. The sensitivity of WUE to SWC stemmed from the responsiveness of T/ET to SWC, especially under high VPD conditions. Conversely, the sensitivity of WUE to VPD emanated from the impact on GPP/T. SWC and VPD exerted distinct effects on ecosystem WUE through different pathways.

Analysis of Child Safety Monitoring System using the SPSS Method

A wearing children's safety system functions as a smart gadget. Accurate and exact real-time location can be used to identify a child's surroundings. This system includes an ambient temperature display, an SOS light, and a distress buzzer. It aids them in locating their child. The system is simple to design. The programmer is designed to establish an approach that is user-friendly on both sides. GPS-equipped smart phones should be available to both parents and kids. The child's location, call history, message history, and communication via their smartphone are all tracked using this software. Safety monitoring systems use a person, rather than a mechanical system (safety monitor), to warn when roofs are six feet or more below the lowest level and are in danger of falling. A safety supervisor must be a competent person to recognize fall hazards and warn workers about them. The device has IoT tracking and a GSM module that allows you to monitor the child at all times. It has countless sensors connected to a CPU and is used to detect the right signals like heart rate, temperature and other dangers and alert the parents. A programmer called system monitoring software keeps track of, logs, and examines all levels of the IT ecosystem's system resources, from operating systems and electronics to the underlying system applications and services, to running user software. At the top Today, 80% of all child abuse cases reported around the globe. The remaining 26% were boys, with 74% being girls. In this world, a daughter goes missing every 40 seconds. Children If one's children's future is the foundation of one's nation, is affected, it affects overall development Nation. Due to abuses, children's emotional and mental stability is affected Destroying and future endeavors. The things that happen to these defenseless kids are not their fault. Parents are therefore accountable for raising their own children. But, since their children's future and career are not considered, parents are compelled to want for money. So, it is challenging to understand them. There are kids everywhere. We offer a setting in our system where this issue is able to be resolved effectively. It makes it simple for parents to follow their kids in real time as if they were close to them and they focus on their own business without any manual intervention. Ratio studies are statistical analyses of data from appraisals and property valuations. Nearly all states utilise them to produce quantitative measure of the proportion of current market price about which individually estimated taxable property is appraised as well as to offer assessment performance indicators. Evaluation parameters taken as Temperature sensor, Pulse sensor, GPS, GSM and Web camera. The Cronbach's Alpha Reliability result. The overall Cronbach's Alpha value for the model is .658 which indicates 66% reliability. From the literature review, the above 50% Cronbach's Alpha value model can be considered for analysis. Software Engineering Defect Prediction the Cronbach's Alpha Reliability result. The overall Cronbach's Alpha value for the model is .658 which indicates 66% reliability. From the literature review, the above 50% Cronbach's Alpha value model can be considered for analysis.

Effects of a Nutraceutical Treatment on the Intestinal Microbiota of Sled Dogs.

Dog sledding is the main discipline of working dogs on snow, consisting of a team of dogs pulling a sled under the guidance of the owner. To carry out this sport, dogs must have adequate nutrition and vitamin and antioxidant supplementation to ensure that the physical effort is optimal. The present study evaluated the effect that sporting activity and stress have on the canine intestinal microbiota by dividing the dogs into two groups: a control group that did not take any nutraceutical products and the treated group to which a nutraceutical product was administered. The nutraceutical administered in this study is used in all cases of canine intestinal dysbiosis in which it is essential to quickly restore a balanced intestinal microbiota. The results obtained show that in dogs not taking the nutraceutical, there is an increase in bacteria, such as Streptococcus spp. and E. coli, considered enteropathogenic to the detriment of beneficial bacterial species such as Faecalibacterium spp., Turicibacter spp., Blautia spp., Fusobacterium spp., and Clostridium hiranonis. Instead, the group of dogs treated with nutraceutical displays a lower amount of enteropathogenic bacteria and a great increase in the other bacterial species considered beneficial for the animal's health. The results obtained in the present study show that Microbiotal cane® can be used in dogs subject to intense sporting activity by preventing severe alterations at intestinal ecosystem levels by maintaining intestinal bacterial composition as balanced as possible.

Effects of amphibian genetic diversity on ecological communities.

The amount of genetic diversity within a population can affect ecological processes at population, community, and ecosystem levels. However, the magnitude, consistency, and scope of these effects are largely unknown. To investigate these issues, we conducted two experiments manipulating the amount of genetic diversity and environmental factors in larval amphibians. The first experiment manipulated wood frog genetic diversity, the presence or absence of caged predators, and competition from leopard frogs to test whether these factors affected survival, growth, and morphology of wood frogs and leopard frogs. The second experiment manipulated wood frog genetic diversity, the presence or absence of uncaged predators, and resource abundance to test whether these factors affected wood frog traits (survival, morphology, growth, development, and behavior) and other components of the ecological community (zooplankton abundance, phytoplankton, periphyton, and bacterial community structure). Genetic diversity did not affect wood frog survival, growth, and development in either experiment. However, genetic diversity did affect the mean morphology of wood frog tadpoles in the first experiment and the abundance and distribution of zooplankton in the second experiment. It did not affect phytoplankton abundance, periphyton abundance, or bacterial community structure. While effect sizes (Cohen's d) of genetic diversity were approximately half those of environment treatments, the greatest effect sizes were for interaction effects between genetic diversity and environment. Our results indicate that genetic diversity can have a large effect on ecological processes, but the direction of those effects is highly dependent upon environmental conditions, and not easily predicted from simple measures of traits.

The Chilean exposome-based system for ecosystems (CHiESS): a framework for national data integration and analytics platform.

The double burden of diseases and scarce resources in developing countries highlight the need to change the conceptualization of health problems and translational research. Contrary to the traditional paradigm focused on genetics, the exposome paradigm proposed in 2005 that complements the genome is an innovative theory. It involves a holistic approach to understanding the complexity of the interactions between the human being’s environment throughout their life and health. This paper outlines a scalable framework for exposome research, integrating diverse data sources for comprehensive public health surveillance and policy support. The Chilean exposome-based system for ecosystems (CHiESS) project proposes a conceptual model based on the ecological and One Health approaches, and the development of a technological dynamic platform for exposome research, which leverages available administrative data routinely collected by national agencies, in clinical records, and by biobanks. CHiESS considers a multilevel exposure for exposome operationalization, including the ecosystem, community, population, and individual levels. CHiESS will include four consecutive stages for development into an informatic platform: (1) environmental data integration and harmonization system, (2) clinical and omics data integration, (3) advanced analytical algorithm development, and (4) visualization interface development and targeted population-based cohort recruitment. The CHiESS platform aims to integrate and harmonize available secondary administrative data and provide a complete geospatial mapping of the external exposome. Additionally, it aims to analyze complex interactions between environmental stressors of the ecosystem and molecular processes of the human being and their effect on human health. Moreover, by identifying exposome-based hotspots, CHiESS allows the targeted and efficient recruitment of population-based cohorts for translational research and impact evaluation. Utilizing advanced technologies such as Artificial Intelligence (AI), Internet of Things (IoT), and blockchain, this framework enhances data security, real-time monitoring, and predictive analytics. The CHiESS model is adaptable for international use, promoting global health collaboration and supporting sustainable development goals.

Comparative toxicity assessment of alternative versus legacy PFAS: Implications for two primary trophic levels in freshwater ecosystems

Perfluoroalkyl substances (PFAS) are common environmental pollutants, but their toxicity framework remains elusive. This research focused on ten PFAS, evaluating their impacts on two ecotoxicologically relevant model organisms from distinct trophic levels: the crustacean Daphnia magna and the unicellular green alga Raphidocelis subcapitata. The results showed a greater sensitivity of R. subcapitata compared to D. magna. However, a 10-day follow-up to the 48 h immobilisation test in D. magna showed delayed mortality, underlining the limitations of relying on EC50 s from standard acute toxicity tests. Among the compounds scrutinized, Perfluorodecanoic acid (PFDA) was the most toxic to R. subcapitata, succeeded by Perfluorooctane sulfonate (PFOS), Perfluorobutanoic acid (PFBA), and Perfluorononanoic acid (PFNA), with the latter being the only one to show an algicidal effect. In the same species, assessment of binary mixtures of the compounds that demonstrated high toxicity in the single evaluation revealed either additive or antagonistic interactions. Remarkably, with an EC50 of 31 mg L-1, the short-chain compound PFBA, tested individually, exhibited toxicity levels akin to the notorious long-chain PFOS, and its harm to freshwater ecosystems cannot be ruled out. Despite mounting toxicological evidence and escalating environmental concentrations, PFBA has received little scientific attention and regulatory stewardship. It is strongly advisable that regulators re-evaluate its use to mitigate potential risks to the environmental and human health.

Trophic effects of jellyfish blooms on fish populations in ecosystems of the coastal waters of China

Jellyfish play an important role in the material cycling and energy flow of food webs, and massive aggregations may have deleterious consequences for local fisheries; yet a theoretical framework of the trophic effects of jellyfish blooms on coastal fisheries is unclear. To address this knowledge gap, we assessed the trophic interactions between cooccurring bloom jellyfish and dominant fish groups (omnivorous fish and piscivorous fish) in the coastal waters of China (CWC) via stable isotope analysis; we subsequently discussed how jellyfish blooms may affect energy flow through coastal ecosystems. Our results indicate a considerable degree of trophic overlap (mean ratio > 65 %) between jellyfish and small omnivorous fish (< 10 cm), highlighting a similarity in feeding habits, while the overlap ratio decreased to <55 % of the large omnivorous fish group (> 10 cm). Relatively higher trophic levels and smaller overlaps of large omnivorous fish were found in the ecosystem with high jellyfish biomass, which suggested that they may reinforce the ontogenetic trophic shift pattern to alleviate the potential for resource competition with jellyfish under conditions of jellyfish explosion. The smallest trophic overlap (< 20 %) highlighted the strong trophic differentiation between jellyfish and piscivorous fish. Additionally, our study suggested that a massive aggregation of jellyfish can negatively influence zooplankton but may not transfer energy further up efficiently, implying a weak trophic coupling between jellyfish and upper-trophic levels in CWC ecosystems. Thus, we speculate that jellyfish play an important role in shaping pathways involved in the energy transfer of food webs and that large blooms may negatively affect fisheries through bottom-up control affecting prey availability. In general, these results hold strong potential to further improve the understanding of the trophic interactions between jellyfish and fish populations. Furthermore, this study provides valuable data for predicting the consequences of jellyfish blooms on ecosystems, and is crucial for ecosystem-based management of coastal fisheries.

The role of Anchoviella spp. as forage species in different stages of reservoir development in the upper Tocantins River, Central Brazil.

We report the spatiotemporal increase in abundance of Anchoviella spp. (Engraulidae) through the diet of fish consumers after reservoir formation in the upper Tocantins River, Central Brazil. The increase in the abundance of this planktivorous fish evidences the growing importance of autochthonous herbivory food chains as the reservoir stabilized in this novel lentic ecosystem. These changes would otherwise remain unnoticed only through fish sampling, highlighting the importance of assessing trophic interactions to understand responses to damming at the community and ecosystem levels.

From Individual Calcifiers to Ecosystem Dynamics: Ocean Acidification Effects on Urchins and Abalone.

A central question in ecology is to what extent do trophic interactions govern the structure and function of communities? This question is becoming more pressing as trophic interactions shift with rapid climate change. Sea urchins and abalone are key invertebrates in the habitats where they reside. Sea urchins are critical members of exemplar trophic cascades in kelp forests due to their impact on kelp establishment and maintenance; yet their populations are controlled by predators, such as sea otters and sunflower sea stars. Abalone compete with urchins for macroalgal food resources and therefore can help regulate urchin populations in kelp forests. Given that both urchin tests and abalone shells used for predator defense are comprised of calcium carbonate, much research has been conducted on the impacts of ocean acidification (OA) on these calcified structures. A growing body of literature has shown that urchin tests are less calcified and break with less force under OA conditions. Less is known about abalone, but their shells also appear to respond negatively to OA. Using kelp forest communities as exemplar ecosystems, we discuss the morphological, biomechanical, and physiological responses to OA in urchins and abalone and consider how these individual level responses scale to trophic interactions and ultimately whole ecosystem processes. Although the impacts of OA on the calcified structures used for defense have been well studied, calcified mechanisms for food consumption, such as the Aristotle's lantern of urchins, are much less understood. Thus, examining both the feeding and defense sides of trophic interactions would greatly improve our understanding of OA responses across individual to ecosystem scales. More generally, measurements of morphological, biomechanical, and physiological responses to OA can be made in individuals to help predict higher level ecological responses, which would greatly contribute to broader predictions of whole ecosystem responses to OA.

Towards agri‐food industry sustainability: Addressing agricultural technology adoption challenges through innovation

AbstractAgriculture is a key economic driver but also a significant contributor to environmental challenges. Despite promotion, uptake of agricultural technologies remains low due to various challenges. This study explores challenges and solutions in the adoption of agricultural technology in Thailand, emphasising its positive impact on agri‐food sector sustainability. We developed a conceptual framework based on technology adoption literature review. Employing multiple case studies, our research uncovers challenges not only at the farmer level but also within the agricultural ecosystem level. Addressing these challenges requires comprehensive changes in physical, digital, and legal infrastructure. Solutions include reshaping farmer mindsets and enhancing awareness of technology benefits. Despite limitations to the agri‐food industry in Thailand, the study offers valuable insights for technology providers and policymakers, contributing to the literature on technology adoption by smallholder farmers. Our findings emphasise the need for sustainability strategies in the agri‐food sector through greater technology uptake to enhance environmental sustainability.

Understanding the influence of digital ecosystems on digital transformation: The OCO (orientation, cooperation, orchestration) theory

AbstractDigital transformation is a complex, multi‐level phenomenon that still challenges research and practice. Recent research has highlighted the influence of digital ecosystems on digital transformation, but we lack knowledge about how this relationship unfolds across the organisational and the ecosystem levels. Following a phenomenon‐based theorising approach and applying a digital resource‐based view, we present the OCO (orientation, cooperation, orchestration) theory of digital transformation. The OCO theory explains the relationship between an organisation's digital transformation and its integration into a digital ecosystem. We uncover and explain three interdependent mechanisms, that is, orientation, cooperation, and orchestration, that are set in motion by cross‐level interactions between the organisational and the ecosystem levels and that centre around digital resources. Our work advances the frontier of multi‐level digital transformation research explaining the influence of a digital ecosystem on digital transformation. We conclude through six propositions that the deeper its digital ecosystem integration, the more likely an organisation's digital transformation is effective. Therewith, we aim at mobilising future digital transformation research to bridge the organisational and the ecosystem levels and provide four future research themes. Our work also encourages practitioners to acknowledge and manage the influence of digital ecosystems on digital transformation.

A systematic review of invasive non-native freshwater bivalves.

The introduction of invasive species has become an increasing environmental problem in freshwater ecosystems due to the high economic and ecological impacts it has generated. This systematic review covers publications from 2010 to 2020, focusing on non-native invasive freshwater bivalves, a particularly relevant and widespread introduced taxonomic group in fresh waters. We collected information on the most studied species, the main objectives of the studies, their geographical location, study duration, and type of research. Furthermore, we focused on assessing the levels of ecological evidence presented, the type of interactions of non-native bivalves with other organisms and the classification of their impacts. A total of 397 publications were retrieved. The studies addressed a total of 17 species of non-native freshwater bivalves; however, most publications focused on the species Corbicula fluminea and Dreissena polymorpha, which are recognised for their widespread distribution and extensive negative impacts. Many other non-native invasive bivalve species have been poorly studied. A high geographical bias was also present, with a considerable lack of studies in developing countries. The most frequent studies had shorter temporal periods, smaller spatial extents, and more observational data, were field-based, and usually evaluated possible ecological impacts at the individual and population levels. There were 94 publications documenting discernible impacts according to the Environmental Impact Classification for Alien Taxa (EICAT). However, 41 of these publications did not provide sufficient data to determine an impact. The most common effects of invasive bivalves on ecosystems were structural alterations, and chemical and physical changes, which are anticipated due to their role as ecosystem engineers. Despite a considerable number of studies in the field and advances in our understanding of some species over the past decade, long-term data and large-scale studies are still needed to understand better the impacts, particularly at the community and ecosystem levels and in less-studied geographic regions. The widespread distribution of several non-native freshwater bivalves, their ongoing introductions, and high ecological and economic impacts demand continued research. Systematic reviews such as this are essential for identifying knowledge gaps and guiding future research to enable a more complete understanding of the ecological implications of invasive bivalves, and the development of effective management strategies.

Damage assessment on the discarded macro-benthic fauna in the Italian striped venus clam (Chamelea gallina) fisheries

The striped venus clam fishery in Italy is carried out by means of hydraulic dredges and it is one of the most important socio- economic activities in the Italian fisheries sector. Dredging has traditionally been considered among fishing activities with the greatest impact at the ecosystem level. Here, therefore, we investigated the disturbance level exerted by dredging on the studied area. Also, damage and mortality rates exerted by dredging on the discarded macro-benthic fauna living associated with Chamelea gallina during the summer season were investigated using a four-level damage scale, given three different haul time duration (3, 6 and 9 minutes). Despite the fact that macro-benthic fauna represented on average only 4.4% of the total catch, the most represented taxa in terms of both abundance and number were Malacostraca, Bivalvia, and Echinoidea. The analysis of the macro-benthic communities’ structure between hauls revealed that the species composition was very similar, and ABC plots together with the Warwick Statistics (W) revealed a moderately disturbed macro-benthic community. No significant difference was found in damage and mortality rates between hauls duration when stratified at taxon or species level. Overall, 61.0% of individuals of the catch were undamaged, whereas 16.1%, 3.7%, and 19.2% displayed slight, intermediate, and severe damage, respectively. We found that soft-shelled or soft-bodied species were the most affected by the harvesting process, whereas thick-shelled or thick-bodied species suffered the slightest damage. In particular, the species suffering major (severe) damage were the sea urchin Echinocardium cordatum (84.6%), the bivalves Mactra stultorum and Polititapes aureus (32.5% and 20.0%, respectively), the crab Liocarcinus vernalis (4.0%), and the sea star Astropecten irregularis (4.8%). The overall mortality rate of all discarded individuals was 22.9%, with E. cordatum showing the highest mortality rate of 96.0%. These findings highlight the importance of guaranteeing the integrity of the entire ecosystem through the adoption of suitable management plans and actions.

Floristic diversity and its relationships with human land use varied regionally during the Holocene

Humans have caused growing levels of ecosystem and diversity changes at a global scale in recent centuries but longer-term diversity trends and how they are affected by human impacts are less well understood. Analysing data from 64,305 pollen samples from 1,763 pollen records revealed substantial community changes (turnover) and reductions in diversity (richness and evenness) in the first ~1,500 to ~4,000 years of the Holocene epoch (starting 11,700 years ago). Turnover and diversity generally increased thereafter, starting ~6,000 to ~1,000 years ago, although the timings, magnitudes and even directions of these changes varied among continents, biomes and sites. Here, modelling these diversity changes, we find that most metrics of biodiversity change are associated with human impacts (anthropogenic land-cover change estimates for the last 8,000 years), often positively but the magnitudes, timings and sometimes directions of associations differed among continents and biomes and sites also varied. Once-forested parts of the world tended to exhibit biodiversity increases while open areas tended to decline. These regionally specific relationships between humans and floristic diversity highlight that human–biodiversity relationships have generated positive diversity responses in some locations and negative responses in others, for over 8,000 years.

Emerging tools and best practices for studying gut microbial community metabolism.

The human gut microbiome vastly extends the set of metabolic reactions catalysed by our own cells, with far-reaching consequences for host health and disease. However, our knowledge of gut microbial metabolism relies on a handful of model organisms, limiting our ability to interpret and predict the metabolism of complex microbial communities. In this Perspective, we discuss emerging tools for analysing and modelling the metabolism of gut microorganisms and for linking microorganisms, pathways and metabolites at the ecosystem level, highlighting promising best practices for researchers. Continued progress in this area will also require infrastructure development to facilitate cross-disciplinary synthesis of scientific findings. Collectively, these efforts can enable a broader and deeper understanding of the workings of the gut ecosystem and open new possibilities for microbiome manipulation and therapy.

Combined effects of herbicides and insecticides reduce biomass of sensitive aquatic invertebrates

The structure and biomass of aquatic invertebrate communities play a crucial role in the matter dynamics of streams. However, biomass is rarely quantified in ecological assessments of streams, and little is known about the environmental and anthropogenic factors that influence it. In this study, we aimed to identify environmental factors that are associated with invertebrate structure and biomass through a monitoring of 25 streams across Germany. We identified invertebrates, assigned them to taxonomic and trait-based groups, and quantified biomass using image-based analysis. We found that insecticide pressure generally reduced the abundance of insecticide-vulnerable populations (R2 = 0.43 applying SPEARpesticides indicator), but not invertebrate biomass. In contrast, herbicide pressure reduced the biomass of several biomass aggregations. Especially, insecticide-sensitive populations, that were directly (algae feeder, R2 = 0.39) or indirectly (predators, R2 = 0.29) dependent on algae, were affected. This indicated a combined effect of possible food shortage due to herbicides and direct insecticide pressure. Specifically, all streams with increased herbicide pressure showed a reduced overall biomass share of Trichoptera from 43 % to 3 % and those of Ephemeroptera from 20 % to 3 % compared to streams grouped by low herbicide pressure. In contrast, insecticide-insensitive Gastropoda increased from 10 % to 45 %, and non-vulnerable leaf-shredding Crustacea increased from 10 % to 22 %. In summary, our results indicate that at the community level, the direct effects of insecticides and the indirect, food-mediated effects of herbicides exert a combined effect on the biomass of sensitive insect groups, thus disrupting food chains at ecosystem level.

Ecosystem Carbon and Nutrient Balances in Short-Rotation Hybrid Aspen Coppice Under Different Thinning Methods

AbstractCoppice plantations have gained a high interest for biofuel production and carbon uptake in short rotation cycles. There is a limited knowledge how such intensive coppice management affects soil fertility and nutrients supply to maintain carbon sink. We studied ecosystem carbon and nutrients balance and allocation during a 5-year period in hybrid aspen coppice under different thinning methods in hemiboreal Estonia. The benchmark value for the changes was defined before the coppice emerged after the clear-cut of the previously planted hybrid aspen plantation. The studied systematical thinning treatments were as follows: corridor thinning with removal of 67% of the trees (CT), cross-corridor thinning with removal of 89% of the trees (CCT), and unthinned (UT) coppice. The UT and CT treatments resulted in a positive carbon balance at the ecosystem level. In all treatments, a decrease of soil acidity, organic C, total N, K, Mg and Mn contents, and an increase of soil Cu and B contents were observed in the 0–20-cm deep layer. The concentrations of leaf N, P, and K were higher in UT than in the two thinning treatments, indicating that the aspens had not entirely recovered from the changed root to shoot ratio 2 years after thinning, whereas the leaf mass fraction of medium- and small-sized trees had already increased. Bioenergy harvest from the UT site in a 5-year rotation would cause 5–18% removal of NPK from the total ecosystem pool. Overall, hybrid aspen coppice showed positive ecosystem carbon balance after the first 5-year period; however, further monitoring of soil properties is needed as we found decrease of soil organic C and nutrients concentrations in short term. Graphical Abstract

Whole-Cell Biosensor for Iron Monitoring as a Potential Tool for Safeguarding Biodiversity in Polar Marine Environments.

Iron is a key micronutrient essential for various essential biological processes. As a consequence, alteration in iron concentration in seawater can deeply influence marine biodiversity. In polar marine environments, where environmental conditions are characterized by low temperatures, the role of iron becomes particularly significant. While iron limitation can negatively influence primary production and nutrient cycling, excessive iron concentrations can lead to harmful algal blooms and oxygen depletion. Furthermore, the growth of certain phytoplankton species can be increased in high-iron-content environments, resulting in altered balance in the marine food web and reduced biodiversity. Although many chemical/physical methods are established for inorganic iron quantification, the determination of the bio-available iron in seawater samples is more suitably carried out using marine microorganisms as biosensors. Despite existing challenges, whole-cell biosensors offer other advantages, such as real-time detection, cost-effectiveness, and ease of manipulation, making them promising tools for monitoring environmental iron levels in polar marine ecosystems. In this review, we discuss fundamental biosensor designs and assemblies, arranging host features, transcription factors, reporter proteins, and detection methods. The progress in the genetic manipulation of iron-responsive regulatory and reporter modules is also addressed to the optimization of the biosensor performance, focusing on the improvement of sensitivity and specificity.