Introduction All-cause craniotomies comprise a significant portion of neurosurgical practice as well as hospital costs. While some instruments are reusable with a fixed cost, price variability for similar single-use instruments exists. A better understanding of these cost variations within cranial procedures can better inform operating physicians to be cost-sensitive stewards. Objective In this study, we examine how single-use items contribute to the overall cost of cranial procedures. Methods A de-identified institutional database containing records of all single-use items from craniotomies between July 1, 2019, and June 30, 2020, was subject to a longitudinal analysis by three independent parties (one senior surgeon, one resident, and one medical student). Four hundred and sixty-nine unique single-use items were identified and classified by function. Similar items were combined, and a range of costs was provided. Three sample cases with sum costs were reviewed for cost division and primary contributors. Results The category with the highest median cost across all cases was non-specialty implants comprising dural onlays, mesh, aneurysm clips, and plates. The category with the lowest median cost was personal protective equipment. The items with the most cost variability were sterile surgical patties due to the variety of sizes and preset multipacks. The proportion of cost generators varies from craniotomy indication. Conclusion While institution dependent, awareness of cost generators in cranial cases is important for economic stewardship. For single-use items, costs are highly variable and not insignificant.Surgeons and neurosurgical departments are responsible for allocating single-use items in a responsibleand efficient manner.