Ecology Of Species Research Articles

Knowledge, attitudes, and conservation challenges for the Lesser Adjutant in Nepal

The knowledge and attitudes of local communities are essential for understanding the distribution, ecology, and pertinent threats to bird species, particularly those existing beyond protected areas. Despite the reliance of large waterbirds like storks on human-dominated landscapes, research in this domain is limited across its range. To address this knowledge gap, we conducted semi-structured interviews with 546 local people across seven districts in the lowlands of Nepal from 2021 to 2022 to insights into people’s knowledge, attitudes, and the conservation challenges faced by the Lesser Adjutant (Leptoptilos javanicus). Our findings showed that people possessed substantial knowledge about the species name and ecology. A majority of the people (89%), agreed with the statement that the Lesser Adjutant contributes to ecosystem services in farmlands, whereas they disagreed with the notions that it is a pest, unattractive species, or possess medicinal values, with 84%, 82%, and 67% respectively holding these views. In addition, 65% of people reported a significant decline in its population, with 67% noting a decrease in distribution range and 49% observing a decline in nesting sites over the past decade, primarily due to food scarcity, habitat loss, the felling of nesting trees, wetland degradation, pesticide use, and hunting, among other factors. A striking knowledge gap was evident, with approximately two-thirds of people unaware of its conservation status, and over 80% highlighting the absence of local awareness initiatives. Nonetheless, majority of the people (95%) expressed strong support for its conservation, emphasizing the need for intensified awareness programs, habitat conservation and management, and the protection of large trees, among other measures, to ensure the well-being of this iconic species beyond the confines of protected areas. Our study provides essential baseline data for researchers and conservation managers to design effective plans and policies for the research and conservation of the Lesser Adjutant.

Exploring the Intricacies of Encephalitis in Mosquito-Borne Diseases through Vector-Host-Pathogen Interactions: A Critical Review

Environmental changes, medication resistance and sociodemographic shifts have all contributed to a dramatic increase in vector-borne diseases in the last 40 years, impacting both people and domestic animals. Pandemics like the Dengue fever epidemic that hit Bangladesh in 2023 show how devastating these illnesses may be on a global scale. The authors of this work stress the significance of comprehending vector-host-pathogen pathways via their examination of arboviruses in Asia. The ecology and biology of Culex, Culiseta and Aedes species in connection to Dengue Virus, Japanese Encephalitis (JE), Eastern Equine Encephalitis (EEE) and Western Equine Encephalitis (WEE) are explored in a thorough literature review that utilizes MeSH terminology. Beginning with the Japanese Encephalitis Virus (JEV), this article examines the virus’s transmission from insects to vertebrates and, inadvertently, to humans as a disease. We then go on to dengue encephalitis, breaking its intricate pathophysiology into parts. This includes aspects like immune-mediated consequences, systemic problems and direct invasion. The Aedes mosquito is a key player in the human-mosquito-human cycle that transmits Dengue Virus (DENV) and the extrinsic incubation time impacts outbreaks. Continuing to focus on the neuroinvasive effects on horses and people, we have Eastern Equine Encephalopathy (EEE). The interaction between birds and Culiseta melanura mosquitoes highlights the transmission of the enzootic cycle. At last, we look at Western Equine Encephalitis (WEE), which WEEV causes and how it affects both horses and people. Importantly, Culex species, which include mosquitoes and birds, serve as vectors in the enzootic cycle. Factors including climate change and international travel are included in the study’s conclusion, which emphasizes the significance of continuing research to monitor and reduce the worldwide effect of these arboviruses.

Seed dormancy and germination ecology of several clover species

This research was carried out to evaluate the effect of (1) different methods of breaking seed dormancy (physical and chemical scarification, high temperature, hot water and after-ripening), and (2) environmental factors (temperature, salinity and water stress) on seed germination of Trifolium angustifolium, T. arvense, T. fragiferum, T. fucatum, T. repens, T. subterraneum and Trifolium sp. All experiments were conducted in the Seed Research Laboratory of Gorgan University of Agricultural Sciences and Natural Resources, Iran, in 2021. All the examined clover species were dormant after harvest, with little germination. Mechanical scarification with sandpaper or sulphuric acid resulted in more than 88 and 52% germination, respectively. Seeds were able to germinate between 5 and 35°C, but the highest germination (≥ 98% on average) occurred at 20°C. Fluctuating temperature regimes were more efficient in improving the germination than constant temperatures. Seeds of clover species, in addition to having a hard seed coat, have a non-deep physiological dormancy. Fresh seeds showed a high sensitivity to water stress compared with after-ripened seeds. However, relative resistance to salinity stress was observed. The results obtained in this research can be used to develop effective sowing strategies and support the successful establishment of these clover species.

Ecological connectivity analysis based on the fish community in Jeongan Stream

This study focused on the longitudinal connectivity analysis, based on fish communities in the aspect of spatial variations of 34 sites from upstream to downstream. The Fish Assessment Index (FAI), based on ecological guilds and species composition, was applied to compare the ecological health among the sampling sites. The total number of 35 fish species, were sampled during the study period with dominant species of <i>Zacco platypus</i> (35.4%) and <i>Zacco koreanus</i> (11.8%). Cluster analysis showed that all sites were categorized as 4 distinct group communities (A, B, C, D). Non-metric multidimensional scaling analysis showed distinct difference between upstream (A, B) and downstream groups (C, D). Indicator Value Analysis (IndVal method), conducted to identify indicator species within each group, indicated that the species favoring upstream environments Rhynchocypris oxycephalus for the Group A and Iksookimia koreensis for the Group B. In contrast, the analysis indicated that the species with a preference for downstream habitats Pseudogobio esocinus for the Group C and Carassius auratus for the Group D. Ecological health, based on the FAI scores, showed a declining pattern toward the downstream, along with an increasing proportion of tolerant species and omnivores within the fish community. Overall these findings suggest the compositions of fish communities, as four groups, were modified by the weir structures and organic matter, nutrient pollutions.

Species Composition and Ecology of Diurnal Herpetofauna in Agroecosystems of the Ecuadorian Chocó

Mode of killing determines the necrotrophic response of oral bacteria

ABSTRACT Background Bacteria respond to changes in their environment, such as nutrient depletion and antimicrobials exposure. Antimicrobials result not only in bacterial death, but also have a hand in determining species abundances and ecology of the oral biofilms. Proximity of dead bacterial cells to living ones is an important environmental change or stress factor. Dead bacteria represent high concentrations of nutrients, such as proteins, lipids, sugars, and nucleic acids. Living bacteria can use these biomasses as a nutrients source, which is termed necrotrophy. Aim This study investigates the effect of exposing living oral bacteria (planktonic and biofilms) to their dead siblings after being killed by heat or hydrogen peroxide. Results Tested bacterial species showed different responses towards the dead cells, depending on the mode of killing, the nutritional value of the culture media, and the the dead cells density. The multispecies oral biofilms showed different responses towards the supplementation of dead cells during biofilm development, while matured biofilms were more resilient. Conclusion This study indicates that dead bacteria resulting from antiseptics use may imbalance the nutrient availability in the oral cavity, resulting in overgrowth of opportunistic species, and hence ecological changes in oral communities, or introducing new bacterial phenotypes.

A current and annotated inventory of the faunal diversity found on the Anna University campus in Tamil Nadu, India

The variety of creatures that are endemic or local to a certain area and that reside there is referred to as faunal diversity. It includes platyhelminths, odonatan, reptiles (predators), hymenopteran, lepidopteran (pollinators), molluscan, avifauna and mammals. An assessment of animal variety explains the food, habitat, ecology, and population of the various species. A survey was carried out to assess the condition of the faunal diversity at Anna University in Chennai. The study's objectives were to list and define the many animal groups belongs to families, genera, and species that inhabit the region, along with information on their habitats and distribution. A total of 85 species, discovered during the course of a year, from May 2023 to November 2023, are included in the study. There were 85 species in 7 phyla. With 55 species, or 64.71% of all species, the Arthropodan has the largest species abundance of any other group. Only one of the 85 species of Platyhelminthes, the hammer-headed worm (Diversibipalium), was found in the vicinity of the university green garden between September and November of 2023 (Vijayan et al., 2023). The observational study found that the area's green spaces, diversity of flora, and water features contributed to its importance as a habitat for a range of animal species. In order to promote biodiversity conservation efforts and offer habitat for animal species, the study highlights the importance of maintaining or growing green spaces at educational institutions.

The mosquitoes (Diptera: Culicidae) of the Mexican Yucatan Peninsula: a comprehensive review on the use of taxonomic names.

The Yucatan Peninsula is a biogeographic province of the Neotropical region which is mostly encompassed by the 3 Mexican states of Campeche, Quintana Roo, and Yucatán. During the development of the International Joint Laboratory ELDORADO (Ecosystem, bioLogical Diversity, habitat mOdifications and Risk of emerging PAthogens and Diseases in MexicO), a French-Mexican collaboration between the IRD (Institut de Recherche pour le Développement) and UNAM (Universidad Nacional Autónoma de México) in Mérida, it became evident that many putative mosquito species names recorded in the Mexican Yucatan Peninsula were misidentifications/misinterpretations or from the uncritical repetition of incorrect literature records. To provide a stronger foundation for future studies, the mosquito fauna of the Mexican Yucatan Peninsula is here comprehensively reviewed using current knowledge of taxonomy, ecology, and distribution of species through extensive bibliographic research, and examination of newly collected specimens. As a result, 90 mosquito species classified among 16 genera and 24 subgenera are recognized to occur in the Mexican Yucatan Peninsula, including 1 new peninsula record and 3 new state records.

The influence of the number and distribution of background points in presence-background species distribution models

Species distribution models (SDMs), which relate recorded observations (presences) and absences or background points to environmental characteristics, are powerful tools used to generate hypotheses about the biogeography, ecology, and conservation of species. Although many researchers have examined the effects of presence and background point distributions on model outputs, they have not systematically evaluated the effects of various methods of background point sampling on the performance of a single model algorithm across many species. Therefore, a consensus on the preferred methods of background point sampling is lacking. Here, we conducted presence-background SDMs for 20 vertebrate species in North America under a variety of background point conditions, varying the number of background points used, the size of the buffer used to constrain the background points around the occurrences, and the percentage of background points sampled within the buffer (“spatial weighting”). We evaluated the accuracy and transferability of the models using Boyce index, overlap with expert-generated range maps, and area overpredicted and underpredicted by the SDM (and AUC for comparability with other studies).SDM performance is highly dependent on the species modelled but is affected by the number and spread of background points. Models with little spatial weighting had high accuracy (overlap values), but extreme extrapolation errors and overprediction. In contrast, SDMs with high transferability (high Boyce index values and low overprediction) had moderate-to-high spatial weighting. These results emphasize the importance of both background points and evaluation metric selection in SDMs. For other, more successful metrics, using many background points with spatial weighting may be preferred for models with large extents. These results can assist researchers in selecting the background point parameters most relevant for their research question, allowing them to fine-tune their hypotheses on the distribution of species through space and time.

Trophic Interactions of European Hake Merluccius merluccius (Linnaeus, 1758) in Benthic Communities off the Moroccan Mediterranean Coastline: Seasonal and Ontogenetic Shifts.

The European hake, Merluccius merluccius (Linnaeus, 1758), is one of the most important resources for Mediterranean fisheries. Due to its pivotal role in energy transfer from lower to higher trophic levels, this species is a crucial component of the ecosystem's functioning. The ecological role of Merluccius merluccius, off the Moroccan Mediterranean Sea (southern Alboran Sea), was investigated, exploring seasonal and ontogenetic shifts, geographical variations in prey composition, and feeding strategy. Between November 2020 and July 2022, a total of 402 hake specimens were collected by oceanographic bottom trawl surveys (MEDITS) that were carried out during warm and cold seasons to assess their diet and feeding habits. The sample was analyzed according to fish sizes and seasons, and qualitative/quantitative feeding indices were calculated. The trophic spectrum of Merluccius merluccius included 24 prey items in total, mainly belonging to Osteichthyes (12), Crustacea (10), Cephalopoda (1), and Polychaeta (1), suggesting a generalist behavior of this predator as in numerous regions of the Mediterranean Sea, with several species that occasionally occurs in its diet. In the Moroccan Mediterranean Sea, Osteichthyes proved to be the most important prey item (%IRI = 78.56) among the different zoological groups, followed by Crustacea (%IRI = 16.22). The other food items were occasionally and randomly consumed, and cannibalism was low (0.8%). Hierarchical cluster analysis and nonmetric multidimensional scaling (nMDS) showed different feeding habits of two main groups separated at 60% similarity: small specimens <10 cm TL, primarily feed on zooplanktonic prey, while medium and large specimens hold a diet based on Osteichthyes with crustaceans. Furthermore, a significant positive relationship between hake and fish prey size was confirmed. Seasonally, mesopelagic Osteichthyes were the main food prey in the summer season, while pelagic species were predominant during the autumn. SIMPER analysis revealed that the prey items contributing the most to the differences between seasons and length classes were Engraulis encrasicolus, Micromesistius poutassou, Boops boops, Macroramphosus scolopax, gobids, Gadiculus argenteus, and most of Crustacea. The diet does not appear to be influenced by sex (>0.05). A trophic level (TROPH) of 4.1 was calculated, indicating that the species is a top predator (quaternary consumers). The TROPH values ranged between 2.58 and 4.38 from juveniles to adults, increasing asymptotically with the size of specimens. In contrast to what has previously been found in other Mediterranean regions, where ichthyophagous hake feed mostly on pelagic Osteichthyes, such as Engraulis encrasicolus, Sardina pilchardus, and Micromesistius poutassou, the study points up the vital role played by Atlantic horse mackerel Trachurus trachurus for hake diet in the Moroccan Mediterranean Sea. Information on the feeding ecology of fish species as provided in this study is essential to improve ecosystem conservation in accordance with multispecies approach to fishery management, leading to a better understanding of the role of hake in the Moroccan Mediterranean Sea demersal communities.

New record and dietary ecology of an endangered amphibian species, Micryletta nigromaculata Poyarkov, Nguyen, Duong, Gorin &amp; Yang, 2018, from Vietnam

The Black-spotted Paddy Frog (Micryletta nigromaculata) was recently discovered from northern Vietnam. Due to its restricted distribution in limestone karst forests and habitat degradation, the endemic species has been listed as “Endangered” in the IUCN Red List. However, knowledge about the natural history of this species is scarce, including data on its dietary ecology. Recent field surveys revealed new populations of M. nigromaculata occurring in Son La Province, northwestern Vietnam based on morphological analysis. Using the stomach-flushing method, we analyzed stomach contents of 45 individuals (21 males and 24 females) from a total of 52 captured frogs because stomachs of six males and one female (13.5%) were empty. The food spectrum of M. nigromaculata comprises 12 types with a total of 691 prey items (687 invertebrate items and four unidentified items), belonging to six insect orders, larvae and Araneae. Similar to other species of the family Microhylidae, M. nigromaculata is also identified as an ant (Formicidae) – termite (Rhinotermitidae) eating specialist with distinctly higher percentages of occurrence frequency (F = 65 %), number of prey items (N = 80 %), and importance (Ix = 75 %) than those of remaining types. The Simpson diversity index of 0.939 shows a high abundance of food prey in the wild, and the Evenness index of 0.536 suggests biased consumption of feeding selection for M. nigromaculata.

&lt;i&gt;Telipogon pillaropatatensis&lt;/i&gt; (Oncidiinae): a new species from the East-Central Andes of Ecuador

A new species, Telipogon pillaropatatensis, is described from the highlands of east-central Ecuador. It is morphologically similar to T. octavioi but differs by having a corolla with longitudinal thick vein lines without reticulations and a column with three dense bundles of equally sized setae. Also, our phylogenetic analyses suggest that they are not closely related. Interestingly, among the analyzed taxa, T. pillaropatatensis appears to have a closer phylogenetic relationship with the highly distinct T. pulcher and T. hausmannianus than with the morphologically similar T. octavioi. The new species has been found in a relatively small area, covering just a few square kilometers in east-central Ecuador. Additionally, we documented remarkable variation in the coloration and size of the corolla. Information regarding the species' habitat, ecology, phylogenetic relationships, and conservation status is also provided.

Diet in phenotypically divergent sympatric species of African weakly electric fish (genus: Campylomormyrus)-A hybrid capture/HTS metabarcoding approach.

Ecological speciation within the mormyrid genus Campylomormyrus resulted in sympatric species exhibiting divergence in their feeding apparatus and electric organ discharge (EOD). This study documents the overall diet of the genus Campylomormyrus and examines the hypothesis that the Campylomormyrus radiation is caused by adaptation to different food sources. We performed diet assessment of five sympatric Campylomormyrus species (C. alces, C. compressirostris, C. curvirostris, C. tshokwe, C. numenius) and their sister taxon Gnathonemus petersii with markedly different snout morphologies and EODs using hybrid capture/HTS DNA metabarcoding of their stomach contents. Our approach allowed for high taxonomic resolution of prey items, including benthic invertebrates, allochthonous invertebrates and vegetation. Comparisons of the diet compositions using quantitative measures and diet overlap indices revealed that all species are able to exploit multiple food niches in their habitats, that is fauna at the bottom, the water surface and the water column. A major part of the diet is larvae of aquatic insects, such as dipterans, coleopterans and trichopterans, known to occur in holes and interstitial spaces of the substrate. The results indicate that different snout morphologies and the associated divergence in the EOD could translate into different prey spectra. This suggests that the diversification in EOD and/or morphology of the feeding apparatus could be under functional adaptation.

Shading and Planting Material Type Effects on Some Physiological and Biochemical Compounds Synthesis in Desmodium adscendens Plants Leaves in the Domestication Phase, Daloa, Côte d'Ivoire

Desmodium adscendens (Sw.) is a plant of the Fabaceae Family, a wild medicinal plant frequently found in industrial plantations, where it is exposed to pesticide residues. This makes species unsuitable for use as a raw material in pharmaceutical industry. To this end, domestication is one of best ways of cleaning up the species. During this process, the evaluation of a few physiological and biochemical compounds would be an indicator for the choice of planting material and the ecology species. Two types of plant material (seeds and stem) grown under three types of shade (accentuated, moderate, no shade) were tested. The leaves of various 90-day-old plants were used to determine physiological and biochemical parameters various. Results showed that chlorophyll a, carotenoids, polyphenols, total sugars, protein, proline and the activities of tyrosine ammonia-lyase, polyphenol oxidase and catalase were higher in leaves with moderate and no shading. However, under heavy shading, chlorophyll b content, phenylalanine ammonia lyase (PAL) and peroxidase enzyme activities were higher. Whatever shading type, the chl (a) content remained lower than that of chl (b). Also, total sugars, proline, ascorbate peroxidase and catalase were more abundant in the leaves of plants grown from stem. In contrast, phenolics, proteins and PPO are more abundant in leaves of seedlings.

Ecotheories, Ecotranslation and the (Re)Discovery of Travel Writings

The end of the twentieth century and the beginning of the twenty-first century brought awareness of ʻnewʼ threats, a ʻnewʼ normal, such as ecological disasters, species extinction, and pandemic infectious diseases. This context has impacted environmental thinking, and literature has not been exempted from these global inquiries and movements. I intend, first and foremost, to outline a theoretical overview of the main expressions and publications of what I refer to as ecotheories in Translation Studies originating from ʻperipheralʼ countries. Secondly, I will show how Brazil is undergoing an ecological shift, particularly through ecotranslation. Finally, I will address, based on internationalization projects of Brazilian universities, the recent interest and need to translate travel narratives about and in the Amazon, written in foreign languages by travelers and mostly not translated into Portuguese.

Conserving localized endemic butterflies through demographic and ecological studies: Polyommatus humedasae

1.Polyommatus humedasae is an endemic species narrowly distributed in a few sites in the Aosta Valley (NW Italy). Similarly to other alpine butterflies, the survival of the species is closely linked to the conservation of semi-natural grasslands.2.Despite the species being known since 1976, this is the first comprehensive data collection and analysis on this species, firstly to understand its distribution. At Pont d'Ael, the species core site, we applied the mark-release-recapture (MRR) method to estimate population size and density. To understand the species ecology, we collected data on preimaginal stages (eggs and larvae), host plants, and vegetation composition.3.The new data on species distribution (with 5 new sites and 2 subpopulations already extinct) permitted to update the extinction risk through IUCN assessment to Critically Endangered (CR). The estimated size (881 individuals) and density (73 N/ha) of the studied population are higher than those of other endemic Polyommatus species. Data suggest the species is monophagous on Onobrychis arenaria and prefers to lay eggs where the host plant is abundant, in ecotonal areas with some shrub cover (average 8 %), high herbaceous cover (average 68 %) and on plants in better physiological conditions.4.For species such as P. humedasae, which are threaten by woody encroachment, climate change, fires and collection, we propose the application of a management plan that includes: 1) mechanical cut of wood species; 2) small herds on a rotational basis to reduce woody encroachment and maintain shrub patches; 3) dissemination events to highlight the population importance.

A New Rupicolous Species from West of Türkiye: Stachys cuhacioglui (Lamiaceae)

Stachys is one of the most diverse genera of the family Lamiaceae and currently consists of 360 species. Here, we describe Stachys cuhacioglui (Lamiaceae) as a new species based on morphological and micromorphological data. The new species was discovered in İzmir province in west of Türkiye. Stachys cuhacioglui differs from S. annua complex by its perennial, chasmophyte habitus, cordate basal leaves, shorter campanulate calyx with recurved teeth, and shorter corolla. The description, etymology, phenology, geographical distribution, IUCN threat category and ecology of the new species are given.

Archaeological evidence of resource utilisation of the great whales over the past two millennia: A systematic review protocol

Archaeological faunal remains provide key insights into human societies in the past, alongside information on previous resource utilisation and exploitation of wildlife populations. The great whales (Mysticete and sperm whales) were hunted unsustainably throughout the 16th - 20th centuries (herein defined as the modern period) leading to large population declines and variable recovery patterns among species. Humans have utilised whales as a resource through carcass scavenging for millennia; however, increasing local and regional ethnographic and archaeological evidence suggests that, prior to the modern period, hunting of the great whales was more common than previously thought; impacts of earlier hunting pressures on the population ecology of many whale species remains relatively unknown. Hunting guided by traditional ecological knowledge may have been sustainable and likely originated in societies that also incorporated opportunistic use of stranded individuals. The collation of georeferenced zooarchaeological data of the great whales between the 1st - 20th centuries CE worldwide will provide insight into the timescale and distribution of resource utilisation of the great whales and how this varied within and between societies, and may have changed over time. By comparing regions of known resource utilisation and breeding and feeding grounds of current-day whale populations, this information will subsequently be used to infer regions where whale populations were possibly lost or extirpated prior to detailed historical records. This systematic review protocol also provides a template for archaeologists, ecologists, and historians interested in using faunal remains to infer historical ecology and resource use of wild animal populations. The transparency of our data collection approach provides opportunities for reproducibility and comparability with future datasets.

Leaf and root traits are partially coordinated but they show contrasting multi-trait-based community trait dispersion patterns in a subtropical forest

Abstract The ecology of plant species relies on the synchronous functioning of leaves and roots, but few studies have simultaneously examined the community trait dispersion (CTD) patterns of both organs. We measured 16 analogous leaf and root traits on 44 co-occurring woody species in a subtropical forest in southern China, aiming to examine whether leaf and root traits were coordinated, organized into parallel trait axes, exhibited similar CTD, and displayed consistent responses in CTD and community-weighted means of (CWM) traits over environmental gradients. While the first axes of leaf and root trait variation similarly exhibited a fast–slow continuum, leaf traits covered a secondary “carbon economics” axis, contrasting to root traits depicting a collaboration axis reflecting species’ mycorrhizal dependency. Analogous leaf and root chemical traits were generally coordinated but less so for morphological traits. At the community level, changes in the CWM of the first axes were generally consistent among organs with more conservative traits found as increasing elevation but not for the second axis. While root traits became thinner and more conservative as soil phosphorus concentration decreased, leaf traits rarely varied. When different trait axes were combined, leaf traits were overdispersed but tended to converge with increased elevation and soil potassium and phosphorus levels, whereas root traits were clustered but tended to diverge along the same gradients. Our study highlights fine filtering of different suites of traits above- and belowground, which in turn might reduce overall niche overlap among species and promote coexistence with diverse functional designs.

Comparing Transcriptomic Responses to Chemicals Across Six Species Using the EcoToxChip RNASeq Database.

The EcoToxChip project includes RNA-sequencing data from experiments involving model (Japanese quail, fathead minnow, African clawed frog) and ecological (double-crested cormorant, rainbow trout, northern leopard frog) species at multiple life stages (whole embryo and adult) exposed to eight chemicals of environmental concern known to perturb a wide range of biological systems (ethinyl estradiol, hexabromocyclododecane, lead, selenomethionine, 17β trenbolone, chlorpyrifos, fluoxetine, and benzo[a]pyrene). The objectives of this short communication were to (1) present and make available this RNA-sequencing database (i.e., 724 samples from 49 experiments) under the FAIR principles (FAIR data are data which meet principles of findability, accessibility, interoperability, and reusability), while also summarizing key meta-data attributes and (2) use ExpressAnalyst (including the Seq2Fun algorithm and EcoOmicsDB) to perform a comparative transcriptomics analysis of this database focusing on baseline and differential transcriptomic changes across species-life stage-chemical combinations. The database is available in NCBI GEO under accession number GSE239776. Across all species, the number of raw reads per sample ranged between 13 and 58 million, with 30% to 79% of clean reads mapped to the "vertebrate" subgroup database in EcoOmicsDB. Principal component analyses of the reads illustrated separation across the three taxonomic groups as well as some between tissue types. The most common differentially expressed gene was CYP1A1 followed by CTSE, FAM20CL, MYC, ST1S3, RIPK4, VTG1, and VIT2. The most common enriched pathways were metabolic pathways, biosynthesis of cofactors and biosynthesis of secondary metabolites, and chemical carcinogenesis, drug metabolism, and metabolism of xenobiotics by cytochrome P450. The RNA-sequencing database in the present study may be used by the research community for multiple purposes, including, for example, cross-species investigations, in-depth analyses of a particular test compound, and transcriptomic meta-analyses. Environ Toxicol Chem 2024;00:1-6. © 2023 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.