Information on essential resource requirements and characteristics of livestock predation is crucial for formulating a better conservation strategy for leopards in a human-dominated landscape. We assessed food habits and characteristics of livestock predation in the human-dominated landscape of South Gujarat, India, using scat analysis (n = 350) and secondary information from forest department reports (n = 780). Leopards consumed 17 prey species, with pigs contributing the most (44%) to the leopards’ diet, while domestic horses contributed the least (1%). Leopards preyed upon four domestic species: goats (47%), cows (43%), buffaloes (6%), and horses (2%). Young individuals of livestock were most commonly targeted by leopards, and livestock species were predominantly preyed upon during the evening or night time. Livestock were most vulnerable to predation when tied in open areas and least vulnerable while grazing. Our results highlight the significant role of pigs as the main prey and their contribution to mitigating livestock predation. Additionally, improving cattle shed structures and implementing night guarding could help reduce livestock predation. This study also addresses the knowledge gap regarding leopard ecology in human-dominated landscapes.