Ecological water replenishment is the main means of ecological restoration in urbanized rivers. The control targets of utilizing water resources, allocating water and restoring the water quality have become the research hotspot. In this study, the Chengnan River Basin in Jiangbei New District of Nanjing was taken as the object, and the ecological water demand threshold in the Chengnan River Basin was evaluated by using the calculation result of the ecological water demand obtained by the improved Tennant method. According to the ecological water replenishment configuration plan, a water quality and quantity coupling model was constructed to simulate and analyze the water quality improvement effects at the control section of the Chengnan River Basin under different guarantee conditions. The results show that the monthly water demand allocation scheme based on the improved Tennant method can be used as the basis for water replenishment of river ecological restoration, and it can effectively improve the water quality of the Chengnan River. The use of 40,000 t / d water replenishment for 11 days can make the Longwangmiao section meet the water quality target control requirements.