Purpose: The purpose of this research was to estimate Patellofemoral Joint Dysfunction in obese postmenopausal women. Methods: Depending on selection criteria, 100 obese postmenopausal women were selected by simple random sampling method. Written consent was taken and a detailed outcome assessment was done by using a Numerical Pain Rating Scale for pain assessment, Range of motion of hip, knee, ankle joint, Waldron Test, Eccentric Step-down Test, and Quadriceps angle. Statistical analysis was done and results were obtained. Findings: Among the 100 participants, 63% of women experienced pain during their daily activities. The mean pain intensity at rest was 2.25 ± 1.91, and during activity, it increased to 3.28 ± 6.60 (p value < 0.0001). The range of motion in the hip, knee, and ankle joints was reduced compared to that of individuals in the same age group without pain. Specifically, Hip extension-adduction, knee extension, and ankle plantarflexion-inversion-eversion had a p value of <0.0001. For hip flexion-abduction-medial and lateral rotation, knee flexion, and ankle dorsiflexion, the p values were 0.0003, 0.1952, 0.0001, 0.003, 0.0392, and 0.0333. Additionally, 35% of women had quadriceps angles greater than 180 degrees, while the remaining 65% had angles less than 180 degrees. 63% of women exhibited crepitus during activities like stair climbing and walking. 52% of women tested positive on the Waldron Test. 60% of women showed positive findings on the Eccentric Step-down Test. Conclusion: It concluded that there is significant Patellofemoral Joint Dysfunction in obese postmenopausal women.