20-hydroxyecdysone Research Articles

Comparative changes of ecdysteroid content in Bombyx mori eggs in diapausing and non-diapausing development

1. 1. Between oviposition and the late gastrula we found that the concentration of ecdysteroids is higher in diapausing eggs than in non-diapausing eggs. 2. 2. Enzymatic hydrolysis, with Helix pomatia juice, of the conjugated ecdysteroids increases the immunoreactivity of the extracts in greater proportion in diapausing eggs. 3. 3. The analysis of developmental variations of the free ecdysteroids in monovoltin strains shows the presence of a rise in ecdysone in relation to the onset of the diapause (at the gastrula stage). 4. 4. Acidic treatment with HCl which prevents the diapause is followed by an increase of free 20-hydroxy ecdysone. 5. 5. The early diapause is characterized by the loss of ecdysteroids in free-form (ecdysteroids are mainly in conjugated form), while late diapause shows an increase of free ecdysteroids in relation to the resumption of embryogenesis.

Phytoecdysteroids of plants of the genus Silene. XIV. Ecdysterone 20-O-benzoate from Silene tatarica

A new ecdysteroid, which has proved to be ecdysterone 20-O-benzoate, has been isolated from the whole plantSilene tatatica (L.) Pers.

Pharmacokinetics of ecdysterone in experiments

Pharmacokinetics of ecdysterone in experiments

The cell biology of Drosophila wing metamorphosis in vitro.

We have examined the metamorphosis of the wing imaginal disc of Drosophila during culture in vitro in the continuous presence of 20-hydroxy ecdysone (0.1 μg/ ml). We find that the sequence of cellular changes in the wing blade during culture closely match those occurring in situ, involving two periods at which the dorsal and ventral surfaces are joined only by cell processes containing trans-alar microtubule arrays. Good pupal and imaginal cuticle secretion is found in this system.

Phytoecdysteroids of plants of the genusSilene. VII. Sileneoside D — ecdysterone 3-O-α-D-galactopyranoside fromSilene brahuica

A phytoecdysteroid, sileneoside D, has been isolated from the roots ofSilene brahuica Boiss. and it has been shown to be ecdysterone 3-O-α-D-galactopyranoside.

The role of the peripodial membrane in the morphogenesis of the eye-antennal disc ofDrosophila melanogaster.

The early morphogenesis of the eye-antennal disc ofDrosophila in response to 20-hydroxy ecdysone involves the curling of the eye anlagen dorsally over the antenna. During this process, the area of the peripodial membrane is substantially reduced. The peripodial membrane is taut at this stage, and if it is cut the curling of the disc cannot continue, and the eye anlagen returns to its original position within one minute of the operation. In contrast, cutting the columnar epithelium between the eye and antennal anlagen does not disrupt curling, but actually facilitates it. During curling, the cells of the peripodial membrane appear healthy, and exhibit basal extensions. We suggest that the curling of the eye is mediated by the conversion of cuboidal peripodial membrane cells into pseudostratified columnar epithelium at the edges of the peripodial membrane. Subsequently, cells of the peripodial membrane secrete first a pupal cuticle, and then an imaginal cuticle.

Phytoecdysteroids of plants of the genusSilene. V. Sileneoside B — An ecdysterone digalactoside fromSilene brahuica

The phytoecdysteroid sileneoside B has been isolated from the roots ofSilene brahuica Boiss.; it is ecdysterone 3,22-di-O-α-D-galactopyranoside.

Metamorphic changes of wild type and mutant Drosophila tissues induced by 20-hydroxy ecdysone in vitro

Wild type (Oregon R) and non-pupariating as well as late-pupariating mutant larval tissues were cultured in vitro up to 5 weeks with and without 20-hydroxy ecdysone (1 μg/ml). The following responses were elicited by the hormone: in the case of wild type tissues detachment of the larval epidermis and muscles from the cuticle; puparial tanning and sclerotization of the larval cuticle; dissociation of the fat body into single cells; inhibition of the movement of the hind intestine. Most of these responses developed within 1 week of culturing. Of the 4 mutants tested, 3 behaved like the wild type. In cultures of ℓ(1) npr-1, however, puparial tanning, disc evagination, and inhibition of the movement of the hind intestine was abnormally weak and the dissociation of fat body was not observed at all. Detachment of the epidermis and muscles as well as formation of the pupal cuticle by disc tissue occurred normally. The results are discussed with respect to the ecdysteroid-induced metamorphosis of the tissues and the autonomy of mutant gene action.

Chemische und chemotaxonomische Untersuchungen von Filices. XXXII. Chemische Untersuchungen der Inhaltsstoffe von Plenasium banksiifolium (Pr.) Pr.

The fronds of Plenasium banksiifolium (PR.) PR. contain stigmastan-3β, 5α-diol-6-one and its C28-homolog, which are previously unreported in nature, together with the known compounds, ecdysone, ecdysterone, astragalin, stigmastan-3β, 5α, 6β-triol and its C28-homolog.

Phytoecdysones ofSerratula xeranthemoides

Ecdysterone and integristerone A have been isolated from the inflorescences ofSerratula xeranthemoides.

Concerning carbon‐13 n.m.r. spectroscopy of 5β‐hydroxylated phytoecdysones

AbstractThe 13C n.m.r. spectra of the 5β‐hydroxylated phytoecdysones polypodine B, muristerone A and kaladasterone are presented and briefly discussed together with the spectrum of makisterone A. Comparisons with previously reported spectra of ecdysone, ecdysterone and poststerone are made and allow a correction for the C‐20/C‐24 assignment for ecdysone.

Phytoecdysones from Plenasium banksiaefolium and Bolbitis subcordata.

From the Fortnosan ferns, Plenasium banksiaefolium and Bolbitis subcordata, the phytoecdysones ponasterone A and ecdysterone, respectively, have been isolated.

Side-chain hydroxylation in the biosynthesis of β-ecdysone (20-hydroxyecdysone) in the blowflyCalliphora stygia

Dans la mouche a viandeCalliphora stygia le 25-hydroxycholesterol et le (22R)-22-hydroxycholesterol sont incorpores a la β-ecdysone (20-hydroxy-ecdysone) beaucoup moins qu'au cholesterol et ne sont donc probablement pas des precurseurs de cette hormone.

20-hydroxyecdysone, what it can do.

20-hydroxyecdysone, what it can do.

Rubrosterone, a metabolite of insect metamorphosing substance from Achyranthes rubrofusca: Synthesis

Rubrosterone, a metabolite of ecdysterols isolated from Achyranthes spp. (Amaranthaceae), has been synthesized by two routes from ecdysterone.

Bioxynthesis of ecdysterone from cholesterol by a plant.

Bioxynthesis of ecdysterone from cholesterol by a plant.

The isolation of ecdysterone from the radix of Achyranthes obtusifolia lam

The isolation of ecdysterone from the radix of Achyranthes obtusifolia lam