Let G be a finite group. The non-commuting graph of G is a simple graph Γ(G) whose vertices are elements of G∖Z(G), where Z(G) is the center of G, and two distinct vertices a and b are joint by an edge if ab≠ba. In this paper, we study the non-commuting graph of the group U6n. The independent number, clique and chromatic numbers of the non-commuting graph of the group U6n, Γ(U6n), are determined. Additionally, the resolving polynomial, total eccentricity and independent polynomials of Γ(U6n) are computed. Finally, the detour and eccentric connectivity indices of Γ(U6n) are found.