Eastern USA Research Articles

Effect of bottled fluoridated water to prevent dental caries in primary teeth: study protocol for a phase 2 parallel-group 3.5-year randomized controlled clinical trial (waterBEST)

BackgroundFluoridation of public water systems is known as a safe and effective strategy for preventing dental caries based on evidence from non-randomized studies. Yet 110 million Americans do not have access to a fluoridated public water system and many others do not drink tap water. This article describes the study protocol for the first randomized controlled trial (RCT) of fluoridated water that assesses its potential dental caries preventive efficacy when delivered in bottles.MethodswaterBEST is a phase 2b proof-of-concept, randomized, quadruple-masked, placebo-controlled, parallel-group trial designed to estimate the potential efficacy of fluoridated versus non-fluoridated bottled water to prevent dental caries incidence in the first 4 years of life. Two hundred children living in eastern North Carolina, USA, and aged 2–6 months at screening are being allocated at random in a 1:1 ratio to receive fluoridated (0.7 mg/L F) or non-fluoridated bottled water sourced from two local public water systems. Throughout the 3.5-year intervention, study water is delivered monthly in 5-gallon bottles to each child’s home with instructions to use it whenever the child consumes water as a beverage or in food preparation. Parents are interviewed quarterly to monitor children’s water consumption and health. At annual visits, the presence of dental caries is evaluated with a dental screening examination. Clippings from fingernails and toenails are collected to quantify fluoride content as a biomarker of total fluoride intake. The primary endpoint is the number of primary tooth surfaces decayed, missing, or filled due to dental caries measured by the study dentist near the time of the child’s fourth birthday. Tooth decay is assessed at the threshold of macroscopic enamel loss. For the primary aim, a least-squares, generalized linear model will estimate efficacy and its one-tailed, upper 80% confidence limit.DiscussionwaterBEST is the first evaluation of a randomized intervention of fluoridated drinking water in bottles to prevent dental caries in the primary dentition. This innovative method of delivering fluoridated water has the potential to prevent early childhood caries in a large segment of the US population that currently does not benefit from fluoridated public water.Trial registration ClinicalTrials.gov NCT04893681. Registered on March 2022. Last update posted on 10 October 2023. https://clinicaltrials.gov/study/NCT04893681?cond=Dental%20Caries%20in%20Children&term=fluoride&locStr=North%20Carolina,%20USA&country=United%20States&state=North%20Carolina&distance=50&rank=1

Properties influencing flux and diatom uptake of mercury and methylmercury from estuarine sediments

Mercury (Hg) is a conspicuous and persistent global pollutant. Ionic Hg can be methylated into noxious methylmercury (CH3Hg), which biomagnifies in marine tropic webs and poses a health risk to humans and organisms. Sediment Hg methylation rates are variable, and the output flux of created CH3Hg are dependent on sediment characteristics and environmental factors. Thus, uncertainties remain about the formation and flux of CH3Hg from sediment, and how this could contribute to the bioaccumulative burden for coastal organisms in shallow ecosystems. Cores were collected from 3 estuarine locations along the Eastern USA to examine how sediments characteristics influence the introduction of Hg and CH3Hg into the base of the food chain. Stable isotopes of inorganic 200Hg and CH3199Hg were injected into sediments of individual cores, with cultured diatoms constrained to overlying waters. Five different treatments were done on duplicate cores, spiked with: (1) no Hg isotopes (control); (2) inorganic 200Hg; (3) CH3199Hg; (4) both 200Hg and CH3199Hg isotopes, (5) both 200Hg and CH3199Hg into overlying waters (not sediment). Experimental cores were incubated for 3 days under temperature and light controlled conditions. These results demonstrate that upper sediments characteristics lead to high variability in Hg cycling. Notably, sediments which contained abundant and peaty organic material (∼28 %LOI), had the highest pore water DOC (3206 μM) and displayed bands of sulfur reducing bacteria yielded the greatest methylation rate (1.97 % day−1) and subsequent diatom uptake of CH3200Hg (cell quota 0.18 amol/cell) in the overlying water.

Projected Increase in Compound Drought and Hot Days over Global Maize Areas under Global Warming

Compound drought and hot events can lead to detrimental impacts on crop yield with grave implications for global and regional food security. Hence, an understanding of how such events will change under unabated global warming is helpful to avoid associated negative impacts and better prepare for them. In this article, we comprehensively analyze the projected changes in compound drought and hot days (CDHDs) occurring within the maize-growing season of 2015–2100 over dynamic global maize areas using 10 downscaled Coupled Model Intercomparison Project Phase 6 (CMIP6) models and four socio-economic scenarios (SSP1-2.6, SSP2-4.5, SSP3-7.0 and SSP5-8.5). The results demonstrate a notable increase in the frequency and severity of CDHDs over global maize areas under all four SSPs, of which SSP5-8.5 has the fastest rise, followed by SSP3-7.0, SSP2-4.5 and SSP1-2.6. By the end of 21st century, the global average frequency and severity of CDHDs will reach 18~68 days and 1.0~2.6. Hotspot regions for CDHDs are mainly found in southern Africa, eastern South America, southern Europe and the eastern USA, where drought and heat show the most widespread increases. The increase in CDHDs will be faster than general hot days so that almost all increments of hot days will be accompanied by droughts in the future; therefore, compound dry and hot stresses will gradually become the predominant form of dry and heat stress on maize growth. The results can be applied to optimize adaptation strategies for mitigating risks from CDHDs on maize production worldwide.

LACK OF SIZE-ASSORTATIVE MATING IN A WIDESPREAD TREEFROG: PSEUDACRIS CRUCIFER IN IOWA

The Spring Peeper (Pseudacris crucifer) is a small treefrog native to much of the Central and Eastern USA. Here, we tested if Spring Peepers exhibit size-assortative mating—non-random mate pairings based on body size—from a population in eastern Iowa. We captured 75 amplecting pairs of Spring Peepers during the 2021 breeding season, measured their body sizes, and marked them for future recognition. We found that while sexual-size dimorphism exists between males and females, there was no evidence for size-assortative mating among amplectant pairs. Our study contributes to other recent work indicating that size-assortative mating is rare among most anurans, and that previous findings of this phenomenon in frogs should be interpreted with caution.

A new look into the impacts of dust radiative effects on the energetics of tropical easterly waves

Abstract. Saharan dust aerosols are often embedded in tropical easterly waves, also known as African easterly waves, and are transported thousands of kilometers across the tropical Atlantic Ocean, reaching the Caribbean Sea, Amazon Basin, and eastern USA. However, due to the complex climate dynamics of west Africa and the eastern tropical Atlantic Ocean, there is still a lack of understanding of how dust particles may influence the development of African easterly waves, which are coupled to deep convective systems over the tropical Atlantic Ocean and in some cases may seed the growth of tropical cyclones. Here we used 22 years of daily satellite observations and reanalysis data to explore the relationships between dust in the Saharan air layer and the development of African easterly waves. Our findings show that dust aerosols not merely are transported by the African easterly jet and the African easterly waves system across the tropical Atlantic Ocean, but also contribute to the changes in the eddy energetics of the African easterly waves. The efficiency of the dust radiative effect in the atmosphere is estimated to be a warming of approximately 20 W m−2 over the ocean and 35 W m−2 over land. This diabatic heating of dust aerosols in the Saharan air layer acts as an additional energy source to increase the growth of the waves. The enhanced diabatic heating of dust leads to an increase in meridional temperature gradients in the baroclinic zone, where eddies extract available potential energy from the mean flow and convert it to eddy kinetic energy. This suggests that diabatic heating of dust aerosols can increase the eddy kinetic energy of the African easterly waves and enhance the baroclinicity of the region. Our findings also show that dust outbreaks over the tropical Atlantic Ocean precede the development of baroclinic waves downstream of the African easterly jet, which suggests that the dust radiative effect has the capability to trigger the generation of the zonal and meridional transient eddies in the system comprising the African easterly jet and African easterly waves.

New geographic and stratigraphic occurrences of the enigmatic extinct lamniform shark, Megalolamna (Lamniformes: Otodontidae), from the eastern USA

ABSTRACT Megalolamna is an extinct lamniform shark genus that is known from Miocene marine deposits. In this study, three new specimens of Megalolamna collected from the eastern USA are newly described, and the diagnosis of the genus is emended to include a previously undocumented dental characteristic. The new specimens are represented by one tooth from the upper Oligocene Chandler Bridge Formation in South Carolina and two isolated teeth from the lower Miocene Calvert Formation in Maryland. The specimens from the Calvert Formation represent the northern-most occurrence of Megalolamna. Although the taxonomic identification of the specimen from the Chandler Bridge Formation is somewhat tentative, it is considered to represent geologically the oldest record of Megalolamna, suggesting the likelihood that the enigmatic genus has its origin in the Palaeogene no later than 23.5 Ma.

Platinum, shock-fractured quartz, microspherules, and meltglass widely distributed in Eastern USA at the Younger Dryas onset (12.8 ka)

Sediment sequences spanning the 12,800-year-old lower Younger Dryas boundary (YDB) were investigated at three widely separated sites in eastern North America (Parsons Island, Maryland, a Newtonville sandpit in southern New Jersey, and Flamingo Bay, South Carolina). All sequences examined exhibit peak abundances in platinum (Pt), microspherules, and meltglass representing the YDB cosmic impact layer resulting from the airbursts/impacts of a fragmented comet ∼12,800 years ago. The evidence is consistent with the Younger Dryas impact hypothesis (YDIH) recorded at ∼50 other sites across North and South America, Europe, Asia, and the Greenland ice sheet. These sequences were also examined for shock-fractured quartz, based on a recent study suggesting that low-shock metamorphism may result from low-altitude bolide airbursts similar to that observed during near-surface atomic detonations. Now, for the first time in a suite of well-separated sites in North America, we report in the YDB the presence of quartz grains exhibiting shock fractures containing amorphous silica. We also find in the YDB high-temperature melted chromferide, zircon, quartz, titanomagnetite, ulvöspinel, magnetite, native iron, and PGEs with equilibrium melting points (∼1,250° to 3,053°C) that rule out anthropogenic origins for YDB microspherules. The collective evidence meets the criteria for classification as an “impact spherule datum.”

Regional differences in deer hunter attitudes and opinions regarding quality deer management (QDM)

ABSTRACT Quality deer management (QDM) is the preeminent framework by which white-tailed deer are managed in the eastern USA. Historic differences surrounding the cultures, traditions, and regulations of deer hunting exist regionally between the Midwest and Southeast, yet no published study has investigated regional differences in attitudes and opinions of QDM among deer hunters. We surveyed 9,301 deer hunters across two Midwestern states (Missouri and Ohio) and two Southeastern states (Louisiana and South Carolina) and found that hunters across regions held similar motivations for deer hunting, as well as similar attitudes and opinions of strategies and expected outcomes of QDM. However, there existed several mismatches between the practices and ideology associated with QDM among Southeastern hunters. Southeastern hunters held greater support for greater antlered bag limits, protection of does, and supplemental feeding. We postulate that these mismatches are largely driven by regional differences in cultures, traditions, and regulations surrounding deer hunting.

Go west: Population genomics reveals unexpected population fluctuations and little gene flow in Western hemisphere populations of the predatory lady beetle, Hippodamia convergens.

Hippodamia convergens-the convergent lady beetle, has been used extensively in augmentative biological control of aphids, thrips, and whiteflies across its native range in North America, and was introduced into South America in the 1950s. Overwintering H. convergens populations from its native western range in the United States are commercially collected and released across its current range in the eastern USA, with little knowledge of the effectiveness of its augmentative biological control. Here we use a novel ddRADseq-based SNP/haplotype discovery approach to estimate its range-wide population diversity, differentiation, and recent evolutionary history. Our results indicate (1) significant population differentiation among eastern USA, western USA, and South American populations of H. convergens, with (2) little to no detectable recent admixture between them, despite repeated population augmentation, and (3) continued recent population size expansion across its range. These results contradict previous findings using microsatellite markers. In light of these new findings, the implications for the effectiveness of augmentative biological control using H. convergens are discussed. Additionally, because quantifying the non-target effects of augmentative biological control is a difficult problem in migratory beetles, our results could serve as a cornerstone in improving and predicting the efficacy of future releases of H. convergens across its range.

Winter Distribution of Golden Eagles in the Eastern USA

Winter Distribution of Golden Eagles in the Eastern USA

An expanded concept of Ceratocystis manginecans and five new species in the Latin American clade of Ceratocystis

ABSTRACT The genus Ceratocystis contains a number of emerging plant pathogens, mostly members of the Latin American Clade (LAC), in which there are several unresolved taxonomic controversies. Among the most important are Brazilian pathogens in the C. fimbriata complex, C. manginecans and C. eucalypticola. Representatives of C. manginecans and C. eucalypticola from India and China, respectively, were shown to be fully interfertile in laboratory matings, and hybrids between the putative species were identified on Punica in India. An Indian tester strain was sexually compatible with representatives of what has been considered C. fimbriata on numerous hosts across Brazil. In this revision of the LAC, the name C. fimbriata is restricted to the widely dispersed Ipomoea strain, and C. manginecans is recognized as a Brazilian species that is important on Mangifera, Eucalyptus, and many other crops. C. mangivora and C. mangicola are also considered synonyms of C. manginecans. Based on phylogenetics and mating studies, two other Brazilian species are recognized: C. atlantica, sp. nov., and C. alfenasii, sp. nov., each with wide host ranges. Three new Caribbean species are recognized based on phylogenetics and earlier inoculation studies: C. costaricensis, sp. nov., on Coffea, C. cubensis, sp. nov., on Spathodea, and C. xanthosomatis, sp. nov., on the vegetatively propagated aroids Xanthosoma and Syngonium. Some of the other Ceratocystis species were based primarily on unique internal transcribed spacer (ITS) rDNA sequences, but the unreliability of rDNA sequences was demonstrated when intraspecific crossing of isolates with differing ITS sequences generated single-ascospore progeny with intragenomic variation in ITS sequences and others with new ITS sequences. Species recognition in Ceratocystis should use phenotype, including intersterility tests, to help identify which lineages are species. Although some species remain under-studied, we recognize 16 species in the LAC, all believed to be native to Latin America, the Caribbean region, or eastern USA.

Long-term mortality burden trends attributed to black carbon and PM2·5 from wildfire emissions across the continental USA from 2000 to 2020: a deep learning modelling study

Long-term improvements in air quality and public health in the continental USA were disrupted over the past decade by increased fire emissions that potentially offset the decrease in anthropogenic emissions. This study aims to estimate trends in black carbon and PM2·5 concentrations and their attributable mortality burden across the USA. In this study, we derived daily concentrations of PM2·5 and its highly toxic black carbon component at a 1-km resolution in the USA from 2000 to 2020 via deep learning that integrated big data from satellites, models, and surface observations. We estimated the annual PM2·5-attributable and black carbon-attributable mortality burden at each 1-km2 grid using concentration-response functions collected from a national cohort study and a meta-analysis study, respectively. We investigated the spatiotemporal linear-regressed trends in PM2·5 and black carbon pollution and their associated premature deaths from 2000 to 2020, and the impact of wildfires on air quality and public health. Our results showed that PM2·5 and black carbon estimates are reliable, with sample-based cross-validated coefficients of determination of 0·82 and 0·80, respectively, for daily estimates (0·97 and 0·95 for monthly estimates). Both PM2·5 and black carbon in the USA showed significantly decreasing trends overall during 2000 to 2020 (22% decrease for PM2·5 and 11% decrease for black carbon), leading to a reduction of around 4200 premature deaths per year (95% CI 2960-5050). However, since 2010, the decreasing trends of fine particles and premature deaths have reversed to increase in the western USA (55% increase in PM2·5, 86% increase in black carbon, and increase of 670 premature deaths [460-810]), while remaining mostly unchanged in the eastern USA. The western USA showed large interannual fluctuations that were attributable to the increasing incidence of wildfires. Furthermore, the black carbon-to-PM2·5 mass ratio increased annually by 2·4% across the USA, mainly due to increasing wildfire emissions in the western USA and more rapid reductions of other components in the eastern USA, suggesting a potential increase in the relative toxicity of PM2·5. 100% of populated areas in the USA have experienced at least one day of PM2·5 pollution exceeding the daily air quality guideline level of 15 μg/m3 during 2000-2020, with 99% experiencing at least 7 days and 85% experiencing at least 30 days. The recent widespread wildfires have greatly increased the daily exposure risks in the western USA, and have also impacted the midwestern USA due to the long-range transport of smoke. Wildfires have become increasingly intensive and frequent in the western USA, resulting in a significant increase in smoke-related emissions in populated areas. This increase is likely to have contributed to a decline in air quality and an increase in attributable mortality. Reducing fire risk via effective policies besides mitigation of climate warming, such as wildfire prevention and management, forest restoration, and new revenue generation, could substantially improve air quality and public health in the coming decades. National Aeronautics and Space Administration (NASA) Applied Science programme, NASA MODIS maintenance programme, NASA MAIA satellite mission programme, NASA GMAO core fund, National Oceanic and Atmospheric Administration (NOAA) GEO-XO project, NOAA Atmospheric Chemistry, Carbon Cycle, and Climate (AC4) programme, and NOAA Educational Partnership Program with Minority Serving Institutions.

Revisiting a cryptic species complex: interspecific gene flow among woodland salamanders in the Blue Ridge Mountains of northern Georgia, USA

Abstract The complex topography of mountainous landscapes can create biogeographical barriers and promote allopatric speciation—even among morphologically cryptic species. However, these biogeographical barriers may not be permanent, and secondary contact between previously independently evolving populations can result in hybridization and introgression. In this study, we used genome-wide single nucleotide polymorphism data to re-examine a classic example of cryptic species in the Appalachian Mountains: the slimy salamander (Plethodon glutinosus) species complex. This group of morphologically similar terrestrial salamanders includes 14 species with parapatric distributions across the eastern USA. We focused on the Chattahoochee slimy salamander (P. chattahoochee) in northern Georgia and used a series of complementary phylogenomic and population genomic analyses to evaluate spatial genetic structure within this species and admixture with at least four other species of parapatric woodland salamanders. Our results highlight the utility of genomic data in clarifying species boundaries and underline the difficulty of species delimitation in organisms with complex evolutionary histories.

Mid-latitude ionospheric disturbances during the major Sudden Stratospheric Warming event of 2018 observed by sub-ionospheric VLF/LF signals

This study examines the impact of a major sudden stratospheric warming (SSW) event in February 2018 on the ionosphere of the mid-to–high latitudes in the Northern Hemisphere. The SSW event commenced on February 11 and persisted for about 18 days. The polar stratospheric temperature at 10 hPa increased by about 50 K during February 17 (daytime) and February 18 (nighttime). To detect lower ionospheric variations, VLF/LF signals from four transmitters received by three receivers in the European sector and one receiver in the eastern USA are analyzed along the five propagation paths. The analysis of the diurnal variations in the European sector indicates a significant fluctuation in signal amplitude exceeding ∼5 dB during nighttime on the most disturbed day (February 13). The 1-h averaged nighttime amplitude also showed a maximum deviation of more than ±2σ on February 13. Notably, this occurrence took place two days after the onset of the SSW event at the European receiver locations. No delay in the occurrence of the maximum anomaly was observed in the westward propagation path in the eastern USA. We also analyzed the critical frequency of the F2 layer (foF2) of the ionosphere during February 2018, recorded by Lowell digisonde stations located near the VLF/LF receivers. The foF2 data shows a significant maximum anomaly of ∼1 MHz during nighttime on February 13 at the European stations, coinciding with the SSW period. This anomaly is consistent with the observed VLF/LF anomaly and aligns with the observed temperature variations. However, the deviations in foF2 at the USA stations are not as prominent as those observed at the European stations.

Offshore wind power around the Iberian Peninsula: variability, complementarity and added value for the power system

The advances in floating offshore wind energy are opening deep sea areas, like the coastal waters of Iberian Peninsula (IP), for the installation of wind farms. The integration of this new energy source in a semi-closed power system with an already high share of variable renewable energies would be facilitated if the potential contribution of offshore wind energy shows reduced variability and limited seasonal variations, as the power demand in IP shows two maxima in winter and summer. The aims of this study are the analysis of temporal variability and spatial complementarity of the potential installation sites, and the identification of an optimal combination of installation areas that minimizes the temporal variability of the aggregated offshore contribution. In order to better capture the marked mesoscale features of winds around the IP, wind data from a very high resolution reanalysis (COSMO-REA6) are used. The analysis considers allowed areas for installation, delimited by the maritime spatial planning. Northern coast areas are characterized by high capacity factors (CFs) and high seasonality, while the lower CFs at the western and southern coasts are compensated by a limited seasonality. Pairwise correlation between the potential areas shows outstanding results, with several negative correlation values within a synoptic scale region, in contrast to other mid-latitude regions like the North Sea or the Eastern USA coast. An optimal aggregation of areas includes at least one area at each of the four main Iberian coasts. A strong reduction of hourly variability is obtained through the resulting combinations, and the seasonality of the aggregated CF is clearly below the values for other offshore areas. Therefore, offshore wind energy can indeed offer an added value for the Iberian power system beyond the high resource amount, reducing the need for storage or backup plants.

FRI147 A Direct-To-Patient Primary Aldosteronism Screening Program In The United States: Implementing Guidelines Nationwide

Abstract Disclosure: J.M. Brown: Consulting Fee; Self; Bayer, Inc. B. Bacare: None. Y.M. Niebuhr: None. L.C. Tsai: None. E.E. Abel: None. B. Honzel: None. A. Vaidya: Consulting Fee; Self; Corcept Therapeutics, HRA Pharmaceuticals, Mineralys. Context: Primary aldosteronism (PA) is a prevalent but grossly underrecognized contributor to the global burden of hypertension, excess cardiovascular and renal disease, and death. Though Endocrine Society guidelines recommend high risk populations who should be screened for PA, fewer than 2% of these at-risk patients are ever tested. Given the poor rates of routine screening within guideline-eligible populations, we designed and implemented a direct-to-patient virtual-based PA screening intervention. Methods: Participants with one or more guideline indications for PA screening were recruited using social media, online recruitment platforms, and online patient portals to participate in a video visit to review medical history and to arrange a local reference lab draw for plasma renin activity (PRA), aldosterone (via LC-MS/MS), and a basic metabolic panel, without adjustment of medications. Screening results were categorized as “Likely Confirmed PA”, “Possible PA”, or “Unlikely PA” based on established diagnostic frameworks. Test results, their interpretations, and possible next clinical steps (i.e., further testing, referral, empiric treatment) were communicated directly to patients and their primary care providers, with annual reminders and follow-up. Results: To date, 3220 participants from 12 states in the eastern USA had been contacted, with 2504 eligible. At the time of submission, 212 participants had completed laboratory assessment, with the remainder pending. 79 (37.3%) participants had resistant hypertension, 95 (44.8%) had hypertension with hypokalemia, 75 (35.4%) had hypertension with sleep apnea, 11 (5.2%) had hypertension with adrenal mass, and 90 (42.5%) had hypertension with a family history of early hypertension and/or stroke. Indicated PA screening had not previously been performed despite access to specialist care: 55% had an endocrinologist, cardiologist, and/or nephrologist. 27.4% of the participants had screening results consistent with either “likely confirmed PA” (7.1%) or “possible PA” (20.3%). Of those with both resistant hypertension and hypokalemia, 35.0% met criteria for “likely confirmed” or “possible” PA. A “likely confirmed” diagnosis of PA was associated with a median aldosterone-to-renin ratio of 33 ng/dL per ng/mL/h [IQR: 24, 76] and prevalent cardiometabolic comorbidities (33.3% had diabetes, 20.0% coronary artery disease, 13.3% heart failure). Conclusions: This ongoing, prospective, virtual-based, direct-to-patient PA screening program identified confirmed or possible PA in up to 27.4% of guideline-eligible participants. By reaching patients directly, rather than relying on routine clinical screening that is known to be dramatically underutilized, this study highlights the unrecognized prevalence of PA and demonstrates a novel approach to identifying this common, morbid, and treatable condition across the USA. Presentation: Friday, June 16, 2023

Trace Elements and Consequent Ecological Risks in Mining-Influenced Streams of Appalachia.

Appalachian (eastern USA) coal surface mines fracture geologic materials, causing release of both major ions and trace elements to water via accelerated weathering. When elevated above natural background, trace elements in streams may produce adverse effects on biota via direct exposure from water and sediment and via dietary exposure in food sources. Other studies have found elevated water concentrations of multiple trace elements in Appalachia's mining-influenced streams. Except for Se, trace-element concentrations in abiotic and biotic media of Appalachian mining-influenced streams are less well known. We analyzed environmental media of headwater streams receiving alkaline waters from Appalachian coal mines for eight trace elements (Al, As, Cd, Cu, Ni, Sr, V, and Zn) and assessed the potential consequent ecological risks. Streamwater, particulate media (sediment, biofilm, leaf detritus), and benthic macroinvertebrates (primary consumers, secondary consumers, crayfish) were sampled from six mining-influenced and three reference streams during low-flow conditions in two seasons. Dissolved Cu, Ni, and Sr were higher in mining-influenced streams than in reference streams; Ni, Sr, and Zn in fine sediments and Ni in macroinvertebrates were also elevated relative to reference-stream levels in samples from mining-influenced streams. Seasonal ratios of mining-influenced stream concentrations to maximum concentrations in reference streams also demonstrated mining-influenced increases for several elements in multiple media. In most media, concentrations of several elements including Ni were correlated positively. All water-column dissolved concentrations were below protective levels, but fine-sediment concentrations of Ni approached or exceeded threshold-effect concentrations in several streams. Further study is warranted for several elements (Cd, Ni, and Zn in biofilms, and V in macroinvertebrates) that approached or exceeded previously established dietary-risk levels. Environ Toxicol Chem 2023;42:2651-2665. © 2023 SETAC.

Whole genome assessment of a declining game bird reveals cryptic genetic structure and insights for population management.

Population genomics applied to game species conservation can help delineate management units, ensure appropriate harvest levels and identify populations needing genetic rescue to safeguard their adaptive potential. The ruffed grouse (Bonasa umbellus) is rapidly declining in much of the eastern USA due to a combination of forest maturation and habitat fragmentation. More recently, mortality from West Nile Virus may have affected connectivity of local populations; however, genetic approaches have never explicitly investigated this issue. In this study, we sequenced 54 individual low-coverage (~5X) grouse genomes to characterize population structure, assess migration rates across the landscape to detect potential barriers to gene flow and identify genomic regions with high differentiation. We identified two genomic clusters with no clear geographic correlation, with large blocks of genomic differentiation associated with chromosomes 4 and 20, likely due to chromosomal inversions. After excluding these putative inversions from the data set, we found weak but nonsignificant signals of population subdivision. Estimated gene flow revealed reduced rates of migration in areas with extensive habitat fragmentation and increased genetic connectivity in areas with less habitat fragmentation. Our findings provide a benchmark for wildlife managers to compare and scale the genetic diversity and structure of ruffed grouse populations in Pennsylvania and across the eastern USA, and we also reveal structural variation in the grouse genome that requires further study to understand its possible effects on individual fitness and population distribution.

Increasing Risks of Future Compound Climate Extremes With Warming Over Global Land Masses

AbstractCompound climate extremes (here referred to compound dry–hot events and compound pluvial–hot events) result in devastating disasters which threaten water‐food‐energy security. However, in a warming scenario, the risk of occurrence, the quantification of uncertainty, and associated drivers of compound climate extremes—particularly compound pluvial–hot events—have not been fully explored. By leveraging climate model large ensembles, it is revealed that the risk of compound climate extremes is projected to increase 2–3 times over most global land masses in future Representative Concentration Pathway (RCP) 8.5 forcing compared with historical forcing. Increased risks of compound climate extremes are mainly attributed to the changes in temperature and changes in dependence between precipitation and temperature, while the change in precipitation contributing to risk of these two compound climate extremes exhibits approximately spatial complementary. In the warming world, the hot spots of compound dry–hot extremes mainly lie in Europe, South Africa, and the Amazon, while those of compound pluvial–hot extremes mostly lie in the eastern USA, eastern and southern Asia, Australia, and central Africa. These findings help stakeholders and decision makers develop a package of climate change adaptation strategies to manage and mitigate the risk of compound climate extremes.

Viruses of Freshwater Mussels during Mass Mortality Events in Oregon and Washington, USA.

Freshwater mussels (Unionida) are globally imperiled, in part due to largely unexplained mass mortality events (MMEs). While recent studies have begun to investigate the possibility that mussel MMEs in the Eastern USA may be caused by infectious diseases, mussels in the Western USA have received relatively little attention in this regard. We conducted a two-year epidemiologic investigation of the role of viruses in ongoing MMEs of the Western pearlshell (Margaritifera falcata) and the Western ridged mussel (Gonidea angulata) in the Chehalis River and Columbia River watersheds in the Western USA. We characterized viromes of mussel hemolymph from 5 locations in 2018 and 2020 using metagenomic methods and identified 557 viruses based on assembled contiguous sequences, most of which are novel. We also characterized the distribution and diversity of a previously identified mussel Gammarhabdovirus related to pathogenic finfish viruses. Overall, we found few consistent associations between viruses and mussel health status. Variation in mussel viromes was most strongly driven by location, with little influence from date, species, or health status, though these variables together only explained ~1/3 of variation in virome composition. Our results demonstrate that Western freshwater mussels host remarkably diverse viromes, but no single virus or combination of viruses appears to be associated with morbidity or mortality during MMEs. Our findings have implications for the conservation of imperiled freshwater mussels, including efforts to enhance natural populations through captive propagation.