Eastern Sites Research Articles

Holocene fire history reconstruction of a mid-elevation mixed-conifer forest in the Eastern Cascades, Washington, USA

Fire histories of mid-elevation mixed-conifer forests are uncommon in the eastern Cascades, limiting our understanding of long-term fire dynamics in these environments. The purpose of this study was to reconstruct the fire and vegetation history for a moist mid-elevation mixed-conifer site, and to determine whether Holocene fire activity in this watershed was intermediate to fire regimes observed at higher and lower elevations in the eastern Cascades. Fire activity and vegetation change was reconstructed using macroscopic charcoal and pollen analysis of sediment core from Long Lake. This site is located ~45 km west of Yakima, WA, and exists in a grand fir-dominated, mixed-conifer forest. Results show low fire activity from ca. 9870 to 6000 cal yr BP, after which time fire increased and remained frequent until ca. 500 cal yr BP. A woodland environment existed at the site in the early Holocene, with the modern coniferous forest establishing ca. 6000–5500 cal yr BP. A mixed-severity fire regime has existed at the site for the past ~6000 years, with both higher- and lower-severity fire episodes occurring on average every ~80–100 years. However, only one fire episode occurred in the Long Lake watershed during the past 500 years, and none within the past ~150 years. Based on a comparison with other eastern Cascade sites, Holocene fire regimes at Long Lake, particularly during the late Holocene, appear to be intermediate between those observed at higher- and lower elevation sites, both in terms of fire severity and frequency.

Environmental factors and occurrence of horseshoe crabs in the northcentral Gulf of Mexico.

This study provides regional-scale data on drivers of horseshoe crab (Limulus polyphemus) presence along the northcentral Gulf of Mexico coast and has implications for understanding habitat suitability for sparse horseshoe crab populations of conservation concern worldwide. To collect baseline data on the relationship between environmental factors and presence of horseshoe crabs, we surveyed four sites from the Fort Morgan peninsula of Mobile Bay, Alabama (AL) to Horn Island, Mississippi (MS). We documented number, size and sex of live animals, molts, and carcasses as metrics of horseshoe crab presence and demographics for two years. Data were compared to in situ and remotely sensed environmental attributes to assess environmental drivers of occurrence during the time of study. Overall, greater evidence of horseshoe crab presence was found at western sites (Petit Bois and Horn Islands) compared to eastern sites (Dauphin Island, Fort Morgan peninsula), mediated by a combination of distance from areas of high freshwater discharge and interannual variation in weather. Higher sex ratios also were found associated with higher occurrence, west of Mobile Bay. Land cover, particularly Bare Land and Estuarine Emergent Wetland classes that are common to western sites, was most predictive of live animal and to some extent carcass occurrence. Our findings suggest that small-scale variation in habitat quality can affect occurrence of horseshoe crabs in sparse populations where density is not a limiting factor. Data from molts and carcasses were informative to supplement live animal data and may be useful to enhance ecological assessment and support conservation and management in regions with sparse populations.

Mobility of the Suzdal Opolye Settlers in 900-1150 AD

The formation of Northeastern Rus in the 10th-11th centuries is usually regarded as a process triggered by intense multicultural interaction and the influx of new settlers from the Dnieper region, Northwestern Rus, and Scandinavia to the Volga-Oka watershed. The dense rural settlement network that existed in 1000-1300, which was recently documented in central Northeastern Rus, and the reconstructed medieval landscapes unambiguously suggest that the prosperity and stability of villages was an important factor in the rise of the region. The level of mobility of the population in Northeastern Rus in the 10th-12th centuries is highly relevant to this issue. This parameter can be assessed using paleodietary data on the isotopic composition of strontium in the dental enamel and bone collagen of individuals buried at medieval cemeteries. The analysis of such samples from a large, rural agglomeration dating to the 10th-early 13th centuries, Shekshovo-9, suggests that this was a culturally diverse and wealthy population, which was part of a trade network. The migration level in this agglomeration was estimated by the results of the mass spectrometric analysis of samples from 24 humans and three animals from the Shekshovo-2 and -9 cemeteries. The reconstructions indicate a high proportion of locals as compared to similar sites in Eastern Europe. No direct relationship was found between the presence of artifacts introduced from other cultures and the isotopic profile of first-generation immigrants. The resulting pattern, indicating a high proportion of native individuals, has no parallels among the 10th-11th century sites in Eastern and Northern Europe represented by comparable data on strontium isotopes.

A Survey of Sediment Fineness and Moisture Content in the Soyang Lake Floodplain Using GPS Data

Abstract Soyang Lake is the largest lake in Republic of Korea, bordering Chuncheon, Yanggu, and Inje in Gangwon Province. It is widely used as an environmental resource for hydropower, flood control, and water supply. Therefore, we conducted a survey of the floodplain of Soyang Lake to analyze the sediments in the area. We used global positioning system (GPS) data and aerial photography to monitor sediment deposits in the Soyang Lake floodplain. Data from three GPS units were compared to determine the accuracy of sampling location measurement. Sediment samples were collected at three sites: two in the eastern region of the floodplain and one in the western region. A total of eight samples were collected: Three samples were collected at 10 cm intervals to a depth of 30 cm from each site of the eastern sampling point, and two samples were collected at depths of 10 and 30 cm at the western sampling point. Samples were collected and analyzed for vertical and horizontal trends in particle size and moisture content. The sizes of the sediment samples ranged from coarse to very coarse sediments with a negative slope, which indicate eastward movement from the breach. The probability of a breach was indicated by the high water content at the eastern side of the floodplain, with the eastern sites showing a higher probability than the western sites. The results of this study indicate that analyses of grain fineness, moisture content, sediment deposits, and sediment removal rates can be used to understand and predict the direction of breach movement and sediment distribution in Soyang Lake.

Screening of possibly anthrax-contaminated burial sites in eastern and southern Ukraine

Aim. The aim of this study was to screen soil samples of 17 anthrax burial sites in Eastern and Southern Ukraine for the presence of B. anthracis. Methods. Soil samples were collected from anthrax grave sites located in Kharkiv, Sumy and Mykolaiv regions (diseased animals dated from 1946 to 2003). Isolation of B. anthracis from collected soil samples was performed with the GABRI method. From single colonies without hemolysis, that were inactivated with peracetic acid- containing 2 % Terralin PAA solution, DNA was extracted and analyzed by qPCR for the presence of chromosomal marker dhp61, as well as the markers pagA and capC located on virulence plasmids pXO1 and pXO2, respectively. Results. Eleven fi eld trips were conducted from July, 2016 to October, 2018 in which 369 soil samples from 17 burial sites in Kharkiv, Sumy and Mykolaiv oblasts were collected from different depths of presumed anthrax carcass sites. In most cases (12 out of 17 cases), the current status of these burial sites was deteriorated and not prop- erly accounted for. It was possible to obrain viable B. anthracis isolate was obtained from 50 cm depth at the grave site near Koviagy village, Valky district, Kharkiv region (49.92373°N, 35.48951°E). This isolate was named KhR/ VD/Kov2-2-05-3 and deposited in the Collection of Animal Infectious Pathogens of the National Scientifi c Center “Institute of Experimental and Clinical Veterinary Medicine”, Kharkiv, Ukraine. The contamination level of soil at the isolation site reached about 10 4 CFU per g as determined by plate counting. qPCR analysis of this isolate identi- fi ed both the dhp61 B. anthracis chromosomal and the pagA virulence plasmid marker. However, the plasmid pXO2 marker, required for capsule-formation could not be detected. Conclusions. The anthrax burial sites were created between the 1920s and 1960s, however, only approximate locations could be found and demarcated. In most cases the status of the sites was unsuitable for sampling. Nevertheless, isolation of B. anthracis in one case in the Valky district shows that old anthrax burial sites (13.500 exist in Ukraine) still pose a risk as potential source of the infection and therefore require more attention and surveillance, for which a surveillance plan will be developed.

Does a Trawl Ban Benefit Commercially Important Decapoda and Stomatopoda in Hong Kong?

Crustaceans were among the most valuable fishery resources in Hong Kong. However, the unrestricted and intensive use of different fishing gears, especially bottom trawling, has led to the depletion of commercially important crustaceans in Hong Kong since the 1980s. This study investigated whether commercial crustaceans recovered after the implementation of a permanent Hong Kong-wide trawl ban that began on December 31, 2012. Standardized field surveys were conducted using a commercial shrimp trawler at two sites in eastern and western waters of Hong Kong before (2004) and after the trawl ban (2013–2014 and 2015–2016) and two sites in southern waters after the trawl ban. Diversity, mean size, abundance, biomass and level of disturbance of commercial crustaceans from the three periods were investigated. The eastern waters exhibited an increased diversity of crustacean assemblages in Inner Tolo, and a higher abundance and biomass of crabs were detected in Outer Tolo after the trawl ban. Reduced disturbance, higher diversity in crustacean assemblages and greater abundance and biomass of predatory crabs were observed after the trawl ban in the outer estuary of western waters, and increased abundance and biomass of shrimp were detected in the inner estuary of western waters. No temporal or negative changes were detected in the southeast and southern waters of Lamma Island. The various responses of crustacean assemblages in Hong Kong waters revealed the critical role of complex interactions among multiple stresses, such as ongoing reclamation works, illegal trawling activities and increased fishing efforts using other (legal) fishing methods.

Microbial Niche Diversification in the Galápagos Archipelago and Its Response to El Niño.

The Galápagos Archipelago is located at the intersection of several major oceanographic features that produce diverse environmental conditions around the islands, and thus has the potential to serve as a natural laboratory for discerning the underlying environmental factors that structure marine microbial communities. Here we used quantitative metagenomics to characterize microbial communities in relation to archipelago marine habitats, and how those populations shift due to substantial environmental changes brought on by El Niño. Environmental conditions such as temperature, salinity, inorganic dissolved nutrients, and dissolved organic carbon (DOC) concentrations varied throughout the archipelago, revealing a diversity of potential microbial niches arising from upwelling, oligotrophic to eutrophic gradients, physical isolation, and potential island mass effects. The volumetric abundances of microbial community members shifted with these environmental changes and revealed several taxonomic indicators of different water masses. This included a transition from a Synechococcus dominated system in the west to an even mix of Synechococcus and Prochlorococcus in the east, mirroring the archipelago’s mesotrophic to oligotrophic and productivity gradients. Several flavobacteria groups displayed characteristic habitat distributions, including enrichment of Polaribacter and Tenacibaculum clades in the relatively nutrient rich western waters, Leeuwenhoekiella spp. that were enriched in the more nutrient-deplete central and eastern sites, and the streamlined MS024-2A group found to be abundant across all sites. During the 2015/16 El Niño event, both environmental conditions and microbial community composition were substantially altered, primarily on the western side of the archipelago due to the reduction of upwelling from the Equatorial Undercurrent. When the upwelling resumed, concentrations of inorganic nutrients and DOC at the western surface sites were more typical of mesopelagic depths. Correspondingly, Synechococcus abundances decreased by an order of magnitude, while groups associated with deeper water masses were enriched, including streamlined roseobacters HTCC2255 and HIMB11, Thioglobacaceae, methylotrophs (Methylophilaceae), archaea (Nitrosopumilaceae), and distinct subpopulations of Pelagibaceriales (SAR11 clade). These results provide a quantitative framework to connect community-wide microbial volumetric abundances to their environmental drivers, and thus incorporation into biogeochemical and ecological models.

Coupled analysis of seawater and sedimentary 231Pa/230Th in the tropical Atlantic

The 231Pa/230Th ratio of seawater and in ocean sediments is a potentially powerful tool in tracing oceanic processes such as the ocean circulation and marine primary productivity. However, 231Pa/230Th reflects the combined signal of multiple controls associated with scavenging and oceanic transport, limiting its use as a paleo-proxy. Given that links between seawater and sedimentary 231Pa and 230Th distributions are crucial for understanding the cycling and removal fluxes of these nuclides, we carry out analysis of 231Pa and 230Th in both seawater (dissolved phase) and modern sediment samples acquired from five depth transects across the northern tropical Atlantic open ocean. Overall, the sediment data support the dominance of 231Pa export in the deep Atlantic Ocean (>2.5 km) associated with the southward advection of North Atlantic Deep Water. However, the seawater data provide evidence for locally enhanced scavenging of 231Pa and 230Th near the western seamounts that is likely to be associated with the influence of hydrothermal activity or episodic formation of transient nepheloid layers. At shallower depths (0.5–1.5 km), paired sediment and seawater 231Pa/230Th data reveal enhanced burial of 231Pa at the eastern and mid-Atlantic sites that is likely related to elevated marine primary productivity and/or influence of boundary scavenging at the African margin, and a net input of 231Pa potentially maintained by the northward advection of intermediate water masses and an additional mechanism of upward diffusion. Our findings provide greater insights into the range of oceanic processes that play a significant role in the cycling of 231Pa and 230Th, with implications for the controls of 231Pa/230Th distribution in the Atlantic Ocean.

How does the stand structure of treeline-forming species shape the treeline ecotone in different regions of the Nepal Himalayas?

Stand structure dynamics are considered as major happenings in any forest as a response to environmental changes. However, this important topic is underrepresented in the treeline studies in the Nepal Himalayas. We aimed to investigate site- as well as species-specific changes in morphometric features (basal diameter, crown cover, density, and height) along the elevational gradient across treeline ecotones in response to recent environmental changes. The stand structure characteristics of Abies spectabilis, Pinus wallichiana, and Betula utilis across the treeline ecotone of three study sites in Eastern (Barun), Central (Manang), and Western (Dhorpatan) Nepal were analyzed to elucidate structural heterogeneities. Altogether, eight transects (20 m × (60–250 m)) across the treeline ecotone were established. Trees of all life forms, trees (> 2 m), saplings (0.5–2 m), and seedlings (< 0.5 m), within each transect were enumerated and sampled for the morphometric features and age. Site-specific and species-specific stand structure dynamics were found. The rate of basal area increment was higher in Barun, but the Manang treeline, despite profound regeneration in recent years, had a low annual basal area increment. Moreover, the altitudinal distribution of age and morphometry were not consistent among those ecotones. Furthermore, intra-specific competition was not significant. The site-specific stand structure dynamics explain why treelines do not respond uniformly to increasing temperature. It invokes, in further studies, the incorporation of the tree’s morphometric adaptation traits, phenotypic plasticity, and interactions between species genotype and the environment.

Population genetic structure of the great star coral, Montastraea cavernosa, across the Cuban archipelago with comparisons between microsatellite and SNP markers

Coral reef habitats surrounding Cuba include relatively healthy, well-developed shallow and mesophotic (30–150 m) scleractinian communities at the cross-currents of the Tropical Western Atlantic (TWA). However, Cuba’s coral communities are not immune to the declines observed throughout the TWA, and there is limited information available regarding genetic connectivity, diversity, and structure among these populations. This represents an immense gap in our understanding of coral ecology and population dynamics at both local and regional scales. To address this gap, we evaluated the population genetic structure of the coral Montastraea cavernosa across eight reef sites surrounding Cuba. Colonies were genotyped using nine microsatellite markers and > 9,000 single nucleotide polymorphism (SNP) markers generated using the 2bRAD approach to assess fine-scale genetic structure across these sites. Both the microsatellite and SNP analyses identified patterns of genetic differentiation among sample populations. While the microsatellite analyses did not identify significant genetic structure across the seven shallow M. cavernosa sampling sites, the SNP analyses revealed significant pairwise population differentiation, suggesting that differentiation is greater between eastern and western sites. This study provides insight into methodological differences between microsatellite and SNP markers including potential trade-offs between marker-specific biases, sample size, sequencing costs, and the ability to resolve subtle patterns of population genetic structure. Furthermore, this study suggests that locations in western Cuba may play important roles in this species’ regional metapopulation dynamics and therefore may merit incorporation into developing international management efforts in addition to the local management the sites receive.

Coastally-adapted: A developing model for coastal Paleoindian sites on the North American eastern continental shelf

Earth has experienced several phases of glacial and interglacial climates over the past 200,000 years. A majority of that time has been under colder than current conditions. During these periods, large amounts of water were frozen at the poles, which lowered sea-levels by as much as 130 m. The climate has been so weighted toward colder conditions, that 90% of human history has taken place during lower than modern sea-levels. The reintroduction of freshwater into the oceans after the last glacial maximum radically changed global sea-levels, inland water tables, and littoral landscapes. Over the last 20,000 years, approximately 20 million km2 of coastal landscape has been submerged worldwide, an area roughly the size of South America. The inundation of these landscapes has created gaps in our understanding of the history of humans directly interacting with the coasts. Here, I briefly review key global Middle and Upper Pleistocene era (162,000–11,500 years ago) archaeological sites and present a model for eastern coastally-adapted Paleoindian sites on the North American Atlantic and Gulf of Mexico (GOM) submerged continental shelf. It is clear from this review that coastally-adapted Paleoindian sites will not likely reflect the quintessential hallmarks of upland Paleoindian sites, with carefully-crafted bifacial projectile points constructed from exotic lithic material, megafaunal remains, ephemeral campsites signifying high mobility. Instead, coastal Paleoindian sites will likely contain micro-blade and utilized flakes technology constructed from locally sourced lithic material in place of projectile points, with few terrestrial fauna, replaced by shellfish middens at longer-term residential sites, within 10 to 12 km of the coast.

Direct evidence for the co-manufacturing of early iron and copper-alloy artifacts in the Caucasus

Models for iron innovation in Eurasia are predicated on understanding the relationship between the bronze and iron industries. In eastern Anatolia, the South Caucasus, and Iran, the absence of scientific analyses of metallurgical debris has obscured the relative chronology, spatial organization, and economic context of early iron and contemporary copper-alloy industries. Survey and excavation at Mtsvane Gora, a fortified hilltop site close to major polymetallic ore sources in the Lesser Caucasus range, recovered metallurgical debris dating to the 8th-6th centuries BC. Optical microscopy, scanning electron microscopy, and energy and wavelength dispersive spectrometry revealed evidence for both iron and copper-alloy metallurgy, including smithing and alloying. Metal particles trapped within clear iron smithing slags were contaminated with copper, arsenic, and tin, suggesting that iron and copper-alloy working took place in the same hearths. The discovery of a small fragment of unprocessed material consisting of pyrite and jarosite, minerals typical of major nearby polymetallic ore deposits, links the secondary smithing and alloying at Mtsvane Gora with nearby mining activities, though the nature of those connections remains unclear. While the earliest iron in the region probably predates the Mtsvane Gora assemblage, the remains date to a period when iron use was still expanding, and they are at present the earliest analytically confirmed, radiocarbon-dated iron metallurgical debris in the Caucasus. The remains are therefore significant for understanding the spread of iron innovation eastward from Anatolia and the Levant. When considered in light of evidence from other Near Eastern sites, the results support a model for innovation in which early iron manufacturing was at least partially integrated with the copper-alloy metallurgical economy.

Elevation and accretion dynamics at historical plots in the Biloxi Marshes, Mississippi Delta

The objectives of this study were to examine changes in accretion and elevation change over periods of up to 15 years for the Biloxi marsh complex (BMC) in southeastern Louisiana, part of the Mississippi Deltaic Plain, identify factors affecting accretionary dynamics, and put these findings in the context of ongoing restoration. We present elevation and accretion data from Surface Elevation Table (SET) and feldspar marker horizon sites first established in 2003. The sites were clustered in two areas (East and West) in the central BMC on the eastern edge of the Mississippi delta. Accretion markers were used in conjunction with elevation measurements to calculate shallow subsidence. These data were analyzed along with similar data from nearby Coastwide Reference Monitoring System (CRMS) sites located around the periphery of the BMC. Elevation decreased at the Western sites by −0.35 ± 0.13 cm/yr, and increased at the Eastern and CRMS sites by 0.40 ± 0.03 cm/yr and 0.72 ± 0.09 cm/yr, respectively. The rate of accretion was similar at the Western (0.49 ± 0.14 cm/yr) and Eastern (0.64 ± 0.07 cm/yr) sites, and over twice as much (1.30 ± 0.11 cm/yr) at the CRMS sites. Shallow subsidence, calculated as the difference between vertical accretion and surface elevation change, was 0.76 ± 0.49 cm/yr at the Western sites, 0.23 ± 0.06 cm/yr at the Eastern sites, and 0.58 ± 0.11 cm/yr at the CRMS sites. These trends are consistent with the observation that sediment is brought in from Chandeleur Sound to the east and is attenuated as deposition occurs across the landscape from east to west, and that levee flank depressions associated with Bayou La Loutre, an abandoned Mississippi River distributary ridge, are causing locally high subsidence in the Western region. Without intervention, these localized areas of the Western region will be submerged within the next several decades at current rates of elevation loss and eustatic sea-level rise, while the Eastern sites and the wetlands on the periphery of the BMC are likely to keep pace with sea level rise well into the second half of this century. These results demonstrate the importance of accurate knowledge of both subsidence and accretionary dynamics in determining coastal wetland sustainability and restoration approaches.

The hazard and unsureness of reducing habitat ranges in response to climate warming for 91 amphibian species in China

Quantifying the unsureness and recognize the hazard of habitats loss from changing climate conditions is important for adaptation of biodiversity to climate warming. The unsureness and hazard of losing habitat ranges for 91 amphibian species in China in response to climate warming were examined by applying climate scenarios of representative concentration pathways and categorization methods of the fuzzy set as well as the Monte Carlo techniques. For non-random scenarios of shifting climate conditions, the abundance of amphibians enhanced in certain sites in northeastern, western China, and declined in certain sites in southeastern, central, eastern, and northern China. For non-stochastic scenarios of changing climate factors, approximately 18–29 species narrowed not more than 20% or 20–40% of their present habitat ranges, and about 74–83 amphibian species occupied over 80% of their overall habitat ranges. Under stochastic scenarios of shifting climatic conditions, the count of species that dwindled different levels of the present or overall habitat ranges declined with improving the possibility; with the likeliness of over 0.6, the number of species that shrunk not more than 20%, 20–40%, 40–60%, 60–80%, and over 80% of the modern habitat areas was roughly 5-11, 4-8, 0-2, 1-3 and 16-24, respectively; the number of species that inhabited not more than 20%, 20–40%, 40–60%, 60–80%, and more than 80% of the overall habitat areas was more or less 1-2, 1-5, 1-6, 0-3 and 25-30, respectively. Approximately 37 amphibian species would be in danger of extinction in accordance with the shrinking habitat ranges due to altering climate conditions, and the measures would be necessary to assist these species to adapt to climate warming.

Colonization history affects heating rates of invasive cane toads

Amphibians in hot climates may be able to avoid high temperatures by controlling their rates of heating. In northern Australia, invasive cane toads (Rhinella marina) experience hot dry conditions in newly-colonized (western) sites but milder conditions in longer-occupied (eastern) sites. Under standardized conditions, toads from western sites heated less rapidly than did conspecifics from an eastern site. The availability of free water slowed heating rates of eastern but not western toads. Thus, the colonization of climatically extreme sites has been accompanied by a rapid shift in the toads’ ability to remain cool under hot conditions, even when free water is not available.

Who were the Hyksos? Challenging traditional narratives using strontium isotope (87Sr/86Sr) analysis of human remains from ancient Egypt

A foreign dynasty, known as the Hyksos, ruled parts of Egypt between c. 1638–1530 BCE. Their origins are thought to be rooted in the Near East, which is supported by architectural features and grave accoutrements of Tell el-Dabca. In this former Hyksos capital in the Eastern Nile Delta, burial culture is characterized by a blend of Egyptian and Near Eastern elements. However, investigations are still ongoing as to where the Hyksos came from and how they rose to power. The aim of this study is to elucidate the question of possible provenience. We present the results of strontium isotope (87Sr/86Sr) ratios of human tooth enamel (n = 75) from Tell el-Dabca, focusing on comparing pre- and during Hyksos rule and sex-based differences. An influx of non-locals can be observed in the pre-Hyksos period (12th and 13th Dynasties, c. 1991–1649 BCE) during the constitution of this important harbor town, while the number of individuals already born in the Delta is larger during the Hyksos period. This is consistent with the supposition that, while the ruling class had Near Eastern origins, the Hyksos’ rise to power was not the result of an invasion, as popularly theorized, but an internal dominance and takeover of foreign elite. There is a preponderance of non-local females suggesting patrilocal residence. We discuss our findings against the current evidence of material culture and historiography, but more investigation in Near Eastern comparative sites has to be conducted to narrow our future search for the actual origins of the Hyksos.

Early Pleistocene fauna of the Olteţ River Valley of Romania: Biochronological and biogeographic implications

The Early Pleistocene of Eurasia is marked by significant climatic, environmental, and faunal shifts and is the time during which Homo first appears in the Eurasian fossil record. To better characterize the environments that were available to these hominins, accurate data regarding the faunal composition of eastern European sites are necessary, as this is the region through which Homo is hypothesized to have dispersed into Europe. Here we present updated and revised taxonomy and biochronology for sites in the Olteţ River Valley (ORV) of central Romania. The site of Grăunceanu is one of the most fossiliferous deposits from this time. Excavations and descriptions of the ORV sites took place during the 1960–1980s, but since that time many taxonomic revisions have been published. Here, we present a reassessment and update of the fauna from the ORV sites. We report several new taxa (e.g., Pachystruthio, Smutsia) and expand the known biogeographic range for other taxa (e.g., Puma pardoides). Our biochronological assessment of this updated taxonomy is consistent with previous reports, though with some refinement. We suggest Grăunceanu is Late Villafranchian (~2.2–1.9 Ma) and compositionally similar to the sites of Saint-Vallier (France) and Vatera (Greece). Similarly, the La Pietriş assemblage was likely deposited >1.7 Ma. The younger site of Fântâna lui Mitilan was deposited after 1.8 Ma, and perhaps as recently as 1.1 Ma. Thus, the ORV faunal assemblages provide an excellent record of nearly the entire span of the Late Villafranchian for both central Romania and eastern Europe.

Foliar functional traits from imaging spectroscopy across biomes in eastern North America

Foliar functional traits are widely used to characterize leaf and canopy properties that drive ecosystem processes and to infer physiological processes in Earth system models. Imaging spectroscopy provides great potential to map foliar traits to characterize continuous functional variation and diversity, but few studies have demonstrated consistent methods for mapping multiple traits across biomes. With airborne imaging spectroscopy data and field data from 19 sites, we developed trait models using partial least squares regression, and mapped 26 foliar traits in seven NEON (National Ecological Observatory Network) ecoregions (domains) including temperate and subtropical forests and grasslands of eastern North America. Model validation accuracy varied among traits (normalized root mean squared error, 9.1-19.4%; coefficient of determination, 0.28-0.82), with phenolic concentration, leaf mass per area and equivalent water thickness performing best across domains. Across all trait maps, 90% of vegetated pixels had reasonable values for one trait, and 28-81% provided high confidence for multiple traits concurrently. Maps of 26 traits and their uncertainties for eastern US NEON sites are available for download, and are being expanded to the western United States and tundra/boreal zone. These data enable better understanding of trait variations and relationships over large areas, calibration of ecosystem models, and assessment of continental-scale functional diversity.

Stable-isotope based trophic metrics reveal early recovery of tropical crustacean assemblages following a trawl ban

Abstract Trawl bans are relatively uncommon, particularly at the level of an entire coastal jurisdiction, and have seldom been studied in the tropics. A permanent territory-wide trawl ban has been implemented in Hong Kong coastal waters since the beginning of 2013. We used isotope-based metrics, in addition to traditional community measurements, to determine whether benthic crustacean assemblages had shown any signs of recovery three years after the trawl ban. We expected increases in species richness, abundance and biomass as well as shifts in trophic structure, including longer food-chain length (FCL) and greater trophic diversity after the ban. We further anticipated that carnivorous crustaceans would have higher mean trophic positions (MTPoC) at the assemblage level, and broader trophic niches at the species level. Sampling was undertaken at six sites in three regions (eastern, western and southern waters) before (in 2012) and after (in 2015) the ban. In general, there were no changes in assemblage total abundance or species richness after the trawl ban, but a decrease in biomass was observed. Isotope-based metrics showed that trophic niches of the crustacean assemblages were, however, broader at all sites after the ban. The FCLs at four sites in the eastern and western waters were longer after the ban, and three of them also showed increased MTPoC. In contrast, declines in FCL and other trophic metrics were recorded at the two southern sites. Among the five most abundant carnivorous crustaceans, all species at one eastern site, three at one western site, and four at three other sites expanded their trophic niches after the trawl ban; no changes were evident at the other eastern site where carnivores were scarce. By using isotope-based trophic metrics, we detected early functional recovery of the crustacean assemblages that cannot be revealed by traditional community analyses, implying that the effectiveness of the trawl ban would be underestimated if the assessment was based on community measurements only. We, therefore, advocate the use of isotope-based trophic metrics in tandem with the community measurements for more comprehensive assessments of ecosystem responses to anthropogenic disturbances and management interventions designed to alleviate them.

Glass Beads from Medieval Gao (Mali): New Analytical Data on Chronology, Sources, and Trade

Abstract Excavations at several archaeological sites in and around Gao have resulted in the recovery of thousands of glass beads presumed to have been acquired from glass bead-producing centers through trade. The bead assemblages cover the period from the eighth to the fourteenth century CE. Here we report on the results of compositional analysis by LA-ICP-MS of 100 beads, permitting comparison with the growing corpus of chemical analyses for glass from African and Near Eastern sites. In this analysis, several compositional groupings are recognized. These include two types of plant-ash soda-lime-silica glass (v-Na-Ca), a mineral soda-lime-silica glass (m-Na-Ca), a high-lime high-alumina (HLHA) glass, a mineral soda-high alumina (m-Na-Al), glass, a plant ash soda-high alumina (v-Na-Al) glass and a high lead composition glass. The reconstruction and dating of depositional contexts suggests a shift in glass sources at the end of the tenth century CE. The issue of source identification is discussed and occurrences at other African sites are mapped, providing new data towards an understanding of trade and exchange networks.